International Journal of Veterinary Science最新文献_第10页

Role of Programmed Cell Death Receptor-1 and Cytotoxic T Lymphocyte-Associated Antigen 4 in Bovine Leukemia Virus Infection 程序性细胞死亡受体1和细胞毒性T淋巴细胞相关抗原4在牛白血病病毒感染中的作用

International Journal of Veterinary Science Pub Date : 2023-01-01 DOI: 10.47278/journal.ijvs/2023.108

引用次数: 0

Canine Osteoarthritic Pain and Joint Inflammation: The Role of Poly Herbal Formulations 犬骨关节炎疼痛和关节炎症:中药复方的作用

International Journal of Veterinary Science Pub Date : 2022-12-31 DOI: 10.14445/24550868/ijvs-v8i3p101

Rohit K J, Bhagwat V G, S. V

引用次数: 0

Bacteriological and Molecular studies on Mycobacterium Bovis in Cattle, with Special Reference to its Antimicrobial Resistance 牛分枝杆菌的细菌学和分子学研究，特别是其抗菌素耐药性研究

International Journal of Veterinary Science Pub Date : 2022-09-01 DOI: 10.47278/journal.ijvs/2022.155a

{"title":"Bacteriological and Molecular studies on Mycobacterium Bovis in Cattle, with Special Reference to its Antimicrobial Resistance","authors":"","doi":"10.47278/journal.ijvs/2022.155a","DOIUrl":"https://doi.org/10.47278/journal.ijvs/2022.155a","url":null,"abstract":"In current study a total of 153 tuberculous lesion samples collected from cattle after PM examination (69 from El-Menufia, 47 from El-Sharkia and 37 from El-Qaliobia governorates) were subjected to bacteriological examination. Eighty-three samples showed the AF bacilli in direct smears stained by ZN stain at a percentage of 54.2% while by the culture method, M. bovis was isolated from 100 (65.4%) samples. Sixty-five isolates, confirmed to be M. bovis by conventional PCR targeting Mpb70, were tested for the sensitivity to anti-tuberculous drugs and 25 isolates showed resistance to at least one tested drug. The overall resistance to EMB was 3.1% and resistance to STR, INH and RIF were 20, 30.8 and 32.3% respectively. MDR was detected in 14 (21.5%) isolates. For DNA sequencing, PCR targeting rpoB gene, conferring rifampicin resistance, was performed on selected 6 isolates and identified three mutations designated as H526Y (4/6), S531L (1/6) and D516V (1/6) and the sequences were submitted to the NCBI GenBank with the accession numbers (OM448574, OM448575, OM448576, OM448577, OM448578 and OM448579). The obtained results are epidemiologically significant and augment the necessity of further investigation on ante-mortem identification of M. bovis infection in cattle to minimize public risk.","PeriodicalId":37665,"journal":{"name":"International Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48346460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical Observations, Hematological Profile, Serological Testing, and Molecular Detection of Erlichia Canis in Veterinary Clinics in Bali, Indonesia 印度尼西亚巴厘岛兽医诊所犬埃利希菌的临床观察、血液学特征、血清学检测和分子检测

International Journal of Veterinary Science Pub Date : 2022-09-01 DOI: 10.47278/journal.ijvs/2022.162a

{"title":"Clinical Observations, Hematological Profile, Serological Testing, and Molecular Detection of Erlichia Canis in Veterinary Clinics in Bali, Indonesia","authors":"","doi":"10.47278/journal.ijvs/2022.162a","DOIUrl":"https://doi.org/10.47278/journal.ijvs/2022.162a","url":null,"abstract":"Ticks of Rhipicephalus species can serve as a vector for transmitting the Zoonotic Ehrlichiosis disease from dogs to humans. In Indonesia, epidemiological data on the prevalence of Ehrlichia canis are very limited. The incidence of Ehrlichiosis is usually reported based on clinical symptoms and serological results. In contrast, accurate diagnosis mainly relies on microscopic examination of a stained blood smear and polymerase chain reaction (PCR). The present study was conducted to evaluate the correlations among clinical observations, hematological profile, serological testing, and molecular detection of E. canis in dogs examined at different animal clinics in Bali, Indonesia. We collected 109 samples from Ehrlichiosis suspected dogs. The disease in these dogs was confirmed through a hematological profile, serologically, and PCR test. The hematological examination was performed with ICUBIO iCell-800Vet. We did a serological examination with Rapid Test BioNote© E. canis. DNA extraction was carried out with DNeasy Blood and Tissue Kit product Qiagen. PCR amplification was performed with Personal Thermal Cycler MJ Mini BIO-RAD. The results revealed that clinical signs such as epistaxis, fever, and pale mucous membranes were strongly associated with serological detection of E. canis. In contrast, none of these signs was significantly correlated with PCR detection of E. canis. Total erythrocytes counts were significantly associated with serologic detection. The total erythrocytes, thrombocytes, and hemoglobin levels were significantly associated with PCR detection. The 16S-rDNA, collected from PCR and amplified, showed the E. canis gene, indicating that E. canis organisms were found in Bali. E canis Bali is one cluster with clusters from South America, Europe, and Asian countries like Thailand, Taiwan, Japan, and Israel. In conclusion, E. canis infection was confirmed through clinical, serological, and molecular approaches in dogs in Bali, Indonesia.","PeriodicalId":37665,"journal":{"name":"International Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48403345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bovine Mastitis in Egypt: Bacterial Etiology and Evaluation of Diagnostic Biomarkers 埃及牛乳腺炎:细菌病因学和诊断生物标志物的评估

International Journal of Veterinary Science Pub Date : 2022-09-01 DOI: 10.47278/journal.ijvs/2022.161

{"title":"Bovine Mastitis in Egypt: Bacterial Etiology and Evaluation of Diagnostic Biomarkers","authors":"","doi":"10.47278/journal.ijvs/2022.161","DOIUrl":"https://doi.org/10.47278/journal.ijvs/2022.161","url":null,"abstract":"In current study a total of 153 tuberculous lesion samples collected from cattle after PM examination (69 from El-Menufia, 47 from El-Sharkia and 37 from El-Qaliobia governorates) were subjected to bacteriological examination. Eighty-three samples showed the AF bacilli in direct smears stained by ZN stain at a percentage of 54.2% while by the culture method, M. bovis was isolated from 100 (65.4%) samples. Sixty-five isolates, confirmed to be M. bovis by conventional PCR targeting Mpb70, were tested for the sensitivity to anti-tuberculous drugs and 25 isolates showed resistance to at least one tested drug. The overall resistance to EMB was 3.1% and resistance to STR, INH and RIF were 20, 30.8 and 32.3% respectively. MDR was detected in 14 (21.5%) isolates. For DNA sequencing, PCR targeting rpoB gene, conferring rifampicin resistance, was performed on selected 6 isolates and identified three mutations designated as H526Y (4/6), S531L (1/6) and D516V (1/6) and the sequences were submitted to the NCBI GenBank with the accession numbers (OM448574, OM448575, OM448576, OM448577, OM448578 and OM448579). The obtained results are epidemiologically significant and augment the necessity of further investigation on ante-mortem identification of M. bovis infection in cattle to minimize public risk.","PeriodicalId":37665,"journal":{"name":"International Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48595357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Phytogenic Substances as Safe Growth Promoters in Poultry Nutrition 植物性物质在家禽营养中的安全生长促进剂作用

International Journal of Veterinary Science Pub Date : 2022-09-01 DOI: 10.47278/journal.ijvs/2022.134

M. Mohamed, H. Hassan

引用次数: 1

Evaluation of Olive Leaves and Pomace Extracts in Growing Rabbit Diets on Productive Performance, Nutrient Digestibility, Carcass Characteristics, Antioxidant Status and Economic Efficiency 生长兔日粮中橄榄叶和果脯提取物对生产性能、养分消化率、胴体特性、抗氧化状况和经济效益的评价

International Journal of Veterinary Science Pub Date : 2022-09-01 DOI: 10.47278/journal.ijvs/2022.155

{"title":"Evaluation of Olive Leaves and Pomace Extracts in Growing Rabbit Diets on Productive Performance, Nutrient Digestibility, Carcass Characteristics, Antioxidant Status and Economic Efficiency","authors":"","doi":"10.47278/journal.ijvs/2022.155","DOIUrl":"https://doi.org/10.47278/journal.ijvs/2022.155","url":null,"abstract":"This study aimed to detect how supplementing olive leaves extract (OLE) and olive pomace extract (OPE) to growing rabbit diets has affected productive performance, nutrient digestibility, carcass characteristics, antioxidant status, and economic efficiency. At 42 days of age, 75 APRI growing rabbits were separated into five groups, 15 rabbits each. Control (without additives), OLE1 (200ppm of OLE), OLE2 (400ppm of OLE), OPE1 (200ppm of OPE), and OPE2 (400ppm of OPE) were the experimental diets. Throughout the trial, we computed feed consumption and increase in body weight on a weekly basis. Compared to the control group, the body weight, weight gain, and feed conversion ratio of rabbits fed OLE or OPE were significantly (P<0.05). The digestibility coefficient of crude protein-enhanced significantly (P<0.05) in rabbits fed OLE or OPE than in control rabbits. In addition to using OLE or OPE, the percentage of the carcass was improved dramatically (P<0.05). Total antioxidant capacity, Superoxide dismutase, and catalase levels were significantly (P<0.05) raised with OLE or OPE, while malondialdehyde levels were lowered. In conclusion, supplementing rabbit diets with 200 or 400ppm of OLE or OPE enhanced productive performance and antioxidant status without any negative effect on their health.","PeriodicalId":37665,"journal":{"name":"International Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41651545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Field Trials to Evaluate Five Fasciolicides against Natural Liver Fluke Infection in Cattle and Sheep in Egypt 5种杀片形虫剂对埃及牛羊天然肝吸虫感染的田间试验评价

International Journal of Veterinary Science Pub Date : 2022-09-01 DOI: 10.47278/journal.ijvs/2022.160

{"title":"Field Trials to Evaluate Five Fasciolicides against Natural Liver Fluke Infection in Cattle and Sheep in Egypt","authors":"","doi":"10.47278/journal.ijvs/2022.160","DOIUrl":"https://doi.org/10.47278/journal.ijvs/2022.160","url":null,"abstract":"Fasciola hepatica, a parasitic trematode, affects cattle and many mammals, including humans. The present study was carried out in Assuit governate, Egypt, over one year from 2018 to 2019, to assess the prevalence of fascioliasis in cattle and sheep. We clinically examined 835 animals (303 cattle and 532 sheep) from different private farms. We performed the fecal examination through a direct smear and did a sedimentation technique. The results demonstrated that fascioliasis was present in 20.8% of cattle and 17.1% of sheep, and the overall prevalence was 18.4%. After assessing associated risk factors, there was a significant association only between sex and infection rate (P<0.05). Other assessed risk factors (species, water, and feeding source) did not affect the infection level (P>0.05). By calculating the odds ratio, the sex was considered as a risk factor as odds ratio (OR) =5.879, 95% confidence interval (CI) 3.699-9.449. Categorized the animals into six groups. We treated each group with either albendazole, triclabendazole, superzole, clorsulon, or rafoxanide. NC group received no treatment. Animals were subjected to clinical and laboratory examination after the second dose's third and sixth week. The recovery percentage in animals treated with albendazole, triclabendazole, and Superzole was 84%, while 84% and 96% in animals treated with clorsulon and rafoxanide, respectively. All the drugs were effective (P<0.05); nonetheless, rafoxanide demonstrated the best recovery percentage (area under curve =0.605 in cattle and =0.615 in sheep).","PeriodicalId":37665,"journal":{"name":"International Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46175260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Water Organic Load and Total Ammonia Nitrogen on Broilers’ Humoral Immune Response Against Newcastle Disease Virus Vaccination in Egypt 水有机负荷和总氨氮对埃及肉仔鸡新城疫病毒免疫应答的影响

International Journal of Veterinary Science Pub Date : 2022-09-01 DOI: 10.47278/journal.ijvs/2022.164

{"title":"Effect of Water Organic Load and Total Ammonia Nitrogen on Broilers’ Humoral Immune Response Against Newcastle Disease Virus Vaccination in Egypt","authors":"","doi":"10.47278/journal.ijvs/2022.164","DOIUrl":"https://doi.org/10.47278/journal.ijvs/2022.164","url":null,"abstract":"Low-quality drinking water (DW) has a negative impact on poultry immunity raising the risk of emerging infections. The current trial used experimentally contaminated broilers’ DW to study its effect on humoral immunity compared to 2 control groups. A total of 450 unsexed 1-day-old Cobb chickens were randomly allocated into 6 groups (75 birds in 5 replicates per group). DW of (T1) had 24 ppm chemical oxygen demand (COD), (T2) 12 ppm COD, (T3) 15 ppm total ammonia nitrogen (TAN), (T4) 5 ppm TAN, (T5) normal tap water (TW), (T6) TW with immunostimulant (IMU FORT®) a day before and after vaccination. Birds were subjected to the NDV DW vaccination program, serum was collected weekly, antibody (Ab) titers were measured along 6 weeks, lymphoid organs somatic indices (OSI) were evaluated after carcass evisceration at the end of the trial (42nd day), and Postmortem lesions were examined. Groups showed differences in Ab titers however, the 3rd and 6th weeks showed significant differences (p≤0.05), at the 3rd week T3 titers were significantly higher than T1, T2 but lower than T4, T6, and at the 6th week T6 titers were significantly higher than T1, T3, T4 but T5 was significantly lower than T3. The lowest OSI was in T3 which records 0.475, 0.133, 0.101 for thymus, bursa, and spleen, respectively while T6 was the highest. Broilers’ DW which has a high organic load (COD) and TAN, significantly decreases Ab titers and OSI. Both good quality DW and immunostimulant supplementation have a positive effect on NDV vaccines’ immune response.","PeriodicalId":37665,"journal":{"name":"International Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45403823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Acute Phase Proteins in Dogs Suffering from Various Diseases 不同疾病犬急性期蛋白的评价

International Journal of Veterinary Science Pub Date : 2022-09-01 DOI: 10.47278/journal.ijvs/2022.167

{"title":"Evaluation of Acute Phase Proteins in Dogs Suffering from Various Diseases","authors":"","doi":"10.47278/journal.ijvs/2022.167","DOIUrl":"https://doi.org/10.47278/journal.ijvs/2022.167","url":null,"abstract":"The proteins of acute phase response are sensitive markers of ongoing inflammatory processes also in dogs with increased production in many infections and organ diseases. However, the magnitude and intensity of increase in their concentrations may differ according to the type of injury or the causative agent of the disease which is not completely understood. Therefore, this study was conducted to determine major acute phase proteins (APP) concentrations in dogs with selected diseases and to compare the values among the various groups of dogs. The study was conducted on serum samples from 89 client-owned dogs with the following diseases: dogs with parvoviral enteritis (n=14), pyometra (n=21), acute pancreatitis (n=14), and babesiosis (n=39). Clinically healthy dogs (n=23) without any abnormal clinical conditions were also included in the study. The concentrations of serum amyloid A (SAA), haptoglobin (Hp), and C-reactive protein (CRP) were analysed in blood serum. The concentrations of all the evaluated APPs differed significantly between the groups of dogs (P<0.001). Although the average concentrations of all parameters were higher in diseased dogs compared to healthy animals, the magnitude of increase differed among the disease groups. Canine parvoviral enteritis and pyometra induced the most marked inflammatory response with the highest mean concentrations of SAA, Hp, and CRP. On the other hand, canine babesiosis was associated with less marked alterations among the evaluated diseases of dogs. The obtained data indicated that the investigated canine disorders were associated with different inflammatory responses, characterized by marked and significant differences in the assessed acute phase reactants.","PeriodicalId":37665,"journal":{"name":"International Journal of Veterinary Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44128672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0