Bovine Mastitis in Egypt: Bacterial Etiology and Evaluation of Diagnostic Biomarkers

Q2 Veterinary

International Journal of Veterinary Science Pub Date : 2022-09-01 DOI:10.47278/journal.ijvs/2022.161

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引用次数: 4

Abstract

In current study a total of 153 tuberculous lesion samples collected from cattle after PM examination (69 from El-Menufia, 47 from El-Sharkia and 37 from El-Qaliobia governorates) were subjected to bacteriological examination. Eighty-three samples showed the AF bacilli in direct smears stained by ZN stain at a percentage of 54.2% while by the culture method, M. bovis was isolated from 100 (65.4%) samples. Sixty-five isolates, confirmed to be M. bovis by conventional PCR targeting Mpb70, were tested for the sensitivity to anti-tuberculous drugs and 25 isolates showed resistance to at least one tested drug. The overall resistance to EMB was 3.1% and resistance to STR, INH and RIF were 20, 30.8 and 32.3% respectively. MDR was detected in 14 (21.5%) isolates. For DNA sequencing, PCR targeting rpoB gene, conferring rifampicin resistance, was performed on selected 6 isolates and identified three mutations designated as H526Y (4/6), S531L (1/6) and D516V (1/6) and the sequences were submitted to the NCBI GenBank with the accession numbers (OM448574, OM448575, OM448576, OM448577, OM448578 and OM448579). The obtained results are epidemiologically significant and augment the necessity of further investigation on ante-mortem identification of M. bovis infection in cattle to minimize public risk.

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埃及牛乳腺炎:细菌病因学和诊断生物标志物的评估

在本研究中，对PM检查后从牛身上采集的153份结核病变样本（69份来自El Menufia，47份来自El Sharkia，37份来自El Qaliobia省）进行了细菌检查。ZN染色的直接涂片中有83个样本显示AF杆菌，阳性率为54.2%，而培养法从100个样本中分离出牛分枝杆菌（65.4%）。通过靶向Mpb70的常规PCR确认为牛分枝杆菌的65个分离株对抗结核药物的敏感性进行了测试，25个分离株显示出对至少一种测试药物的耐药性。对EMB的总体抗性为3.1%，对STR、INH和RIF的抗性分别为20%、30.8%和32.3%。在14株（21.5%）分离株中检测到MDR。对于DNA测序，对选定的6个分离株进行针对rpoB基因的PCR，赋予利福平耐药性，并鉴定出三个突变，分别命名为H526Y（4/6）、S531L（1/6）和D516V（1/6。所获得的结果具有流行病学意义，并增加了对牛感染牛分枝杆菌的尸检鉴定进行进一步调查的必要性，以将公共风险降至最低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Veterinary Science Veterinary-Veterinary (all)

CiteScore

2.40

自引率

0.00%

发文量

181

期刊介绍： “International Journal of Veterinary Science” (IJVS) is an online international peer reviewed open access journal which publishes original research papers. Its scope is broad and international, covering a wide range of research areas including: anatomy, biochemistry, biotechnology, clinical medicine and surgery, Theriogenology, physiology, pharmacology, pathology, microbiology and immunology, parasitology, epidemiology, breeding and genetic, feed and nutrition, wild life, animal products and their processing. Original research papers, review articles, extension articles, clinical articles and short communications on the production and diseases of animals, including studies in comparative medicine is published in this journal. Only those manuscripts are considered for publication, the contents of which have not been published and are not being considered for publication in any other journal.