BioImpacts : BI最新文献_第5页

Electrosprayed polymeric nanobeads and nanofibers of modafinil: preparation, characterization, and drug release studies 莫达非尼的电喷涂聚合物纳米球和纳米纤维:制备、表征和药物释放研究

BioImpacts : BI Pub Date : 2019-04-15 DOI: 10.15171/bi.2019.22

K. Adibkia, Sevil Selselehjonban, S. Emami, K. Osouli-Bostanabad, M. Barzegar-Jalali

{"title":"Electrosprayed polymeric nanobeads and nanofibers of modafinil: preparation, characterization, and drug release studies","authors":"K. Adibkia, Sevil Selselehjonban, S. Emami, K. Osouli-Bostanabad, M. Barzegar-Jalali","doi":"10.15171/bi.2019.22","DOIUrl":"https://doi.org/10.15171/bi.2019.22","url":null,"abstract":"Introduction: Modafinil (MDF) is used orally for the treatment of attention-deficit/hyperactivity disorder and narcolepsy. It holds low solubility and high permeability; therefore, improving its dissolution properties by preparing nanoformulations can be a promising approach to enhance its oral absorption. Our aims were to prepare and characterize MDF-Eudragit® RS100 (MDF-ERS) nanoparticles by electrospray technique. Methods: Electrosprayed nanoparticles were fabricated by varying MDF to ERS ratios and concentrations. The formulations were characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), powder X-ray diffraction (PXRD), and Fourier-transform infrared spectroscopy (FTIR). Release studies were performed on nanoparticles, physical mixtures, and raw MDF. The release data were fitted to different models to understand the mechanism of the drug release. Results: Electrospraying of MDF and ERS solution resulted in the preparation of nonobeads or nanofibers, and the particulate characteristics of the obtained products were largely controlled by the polymer amount in the solution. PXRD and thermal analyses showed that MDF was an amorphous phase in the structures of nanoparticles. Using FTIR, no interaction was observed between MDF and ERS in nanoparticles. Nanoparticles showed biphasic release profiles and the order of dissolution rates was: nanofibers>MDF>nanobeads. The well-fitted model was Weibull model, indicating a Fickian diffusion as the main mechanism of release. Conclusion: The results suggest that by optimization of variables such as solution concentration of MDF-ERS nanofibers and nanobeads with higher dissolution rates can be made by electrospray. Electrospray deposition as a simple, continuous, and surfactant free method is an excellent choice for preparation of drug loaded polymeric nanoparticles.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123187226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

The effects of natural nano-sized clinoptilolite and Nigella sativa supplementation on serum bone markers in diabetic rats 天然纳米斜发沸石和黑草对糖尿病大鼠血清骨标志物的影响

BioImpacts : BI Pub Date : 2019-04-15 DOI: 10.15171/bi.2019.21

Yalda Salari Lak, S. Khorram, Mehran Mesgari Abbasi, M. Asghari-Jafarabadi, A. Tarighat-Esfanjani, Elahe Bazri, H. Omidi

{"title":"The effects of natural nano-sized clinoptilolite and Nigella sativa supplementation on serum bone markers in diabetic rats","authors":"Yalda Salari Lak, S. Khorram, Mehran Mesgari Abbasi, M. Asghari-Jafarabadi, A. Tarighat-Esfanjani, Elahe Bazri, H. Omidi","doi":"10.15171/bi.2019.21","DOIUrl":"https://doi.org/10.15171/bi.2019.21","url":null,"abstract":"Introduction: Many studies confirm that diabetes mellitus is associated with higher risks of bone fracture. The beneficial effects of Nigella sativa (NS) and clinoptilolite in preventing/reducing some diabetes-related disorders have been shown. This study was conducted to examine the effects of separate and concurrent supplementation of natural nano-sized clinoptilolite (NCLN) and NS on serum bone markers in rats with type 2 diabetes. Methods: A total of 42 (case=36 and control=6) adult male Wistar rats were divided into 2 groups: diabetic and non-diabetic. An oral glucose tolerance test and a homeostatic model assessment of insulin resistance (HOMA-IR) test were conducted to confirm diabetes. Then, the diabetic group was divided into 4 subgroups: [1] control (n=9), [2] NS 1%/food (n=9), [3] NCLN 2%/food (n=9), [4] NS 1%/food + NCLN 2%/food (n=9). After 7 weeks, serum levels of bone markers were determined using ELISA kits. Results: Analysis showed that serum levels of alkaline phosphatase (ALP) in the NCLN group (1318.6 ± 217.5 U/L) was significantly (P<0.05) higher than other intervented groups. On the other hand, serum levels of calcium in NCLN+NS group (10.8 ± 2.6 mg/dL) were higher (P=0.027) compared to all other study groups. However, rats in the NS group had higher (535.8 ± 49.3 pg/mL) PTH (P<0.0001) compared to other supplementation groups. There were no significant differences in vitamin D and osteoprotegerin. Conclusion: The results of the current study suggest that bone mineralization may be affected by concurrent use of NS and NCLN through influencing calcium circulation. Moreover, dietary NS administration is strongly related to an augmented level of PTH.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124014906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Manifestation of hemispheric laterality in chewing side preference and handedness 咀嚼侧边偏好和惯用手的半球偏侧表现

BioImpacts : BI Pub Date : 2019-04-15 DOI: 10.15171/bi.2019.23

S. Khamnei, Seyyed-Reza Sadat-Ebrahimi, S. Salarilak, Siavash Savadi Oskoee, Y. Houshyar, S. Shakouri, Y. Salekzamani, M. Zamanlu

引用次数: 5

A short-term plastic adherence incubation of the stromal vascular fraction leads to a predictable GMP-compliant cell-product 基质血管部分的短期塑料粘附培养可获得可预测的符合gmp的细胞产物

BioImpacts : BI Pub Date : 2019-03-25 DOI: 10.15171/bi.2019.20

Stephan Born, M. Dörfel, P. Hartjen, Seyed Ali Haschemi Yekani, Julia Luecke, J. Meutsch, J. Westphal, M. Birkelbach, R. Köhnke, R. Smeets, M. Krueger

{"title":"A short-term plastic adherence incubation of the stromal vascular fraction leads to a predictable GMP-compliant cell-product","authors":"Stephan Born, M. Dörfel, P. Hartjen, Seyed Ali Haschemi Yekani, Julia Luecke, J. Meutsch, J. Westphal, M. Birkelbach, R. Köhnke, R. Smeets, M. Krueger","doi":"10.15171/bi.2019.20","DOIUrl":"https://doi.org/10.15171/bi.2019.20","url":null,"abstract":"Introduction:Mesenchymal stromal/stem cells (MSCs) derived from fat tissue are an encouraging tool for regenerative medicine. They share properties similar to the bone marrow-derived MSCs, but the amount of MSCs per gram of fat tissue is 500x higher. The fat tissue can easily be digested by collagenase, releasing a heterogeneous cell fraction called stromal vascular fraction (SVF) which contains a variable amount of stromal/stem cells. In Europe, cell products like the SVF derived from fat tissue are considered advanced therapy medicinal product (ATMPs). As a consequence, the manufacturing process has to be approved via GMP-compliant process validation. The problem of the process validation for SVF is the heterogeneity of this fraction. Methods: Here, we modified existing purification strategies by adding an additional plastic adherence incubation of maximal 20 hours after SVF isolation. The resulting cell fraction was characterized and compared to SVF as well as cultivated adipose-derived stromal/stem cells (ASCs) with respect to viability and cell yield, the expression of surface markers, differentiation potential and cytokine expression. Results: Short-term incubation significantly reduced the heterogeneity of the resulting cell fraction compared to SVF. The cells were able to differentiate into adipocytes, chondrocytes, and osteoblasts. More importantly, they expressed trophic proteins which have been previously associated with the beneficial effects of MSCs. Furthermore, GMP compliance of the production process described herein was acknowledged by the national regulatory agencies (DE_BB_01_GMP_2017_1018). Conclusion: Addition of a short purification-step after the SVF isolation is a cheap and fast strategy to isolate a homogeneous uncultivated GMP-compliant cell fraction of ASCs.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"160 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134486605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Spectroscopic overview of quercetin and its Cu(II) complex interaction with serum albumins 槲皮素及其Cu(II)复合物与血清白蛋白相互作用的光谱综述

BioImpacts : BI Pub Date : 2019-03-25 DOI: 10.15171/bi.2019.15

P. Mondal, A. Bose

引用次数: 14

Functional expression and impact of testis-specific gene antigen 10 in breast cancer: a combined in vitro and in silico analysis 睾丸特异性基因抗原10在乳腺癌中的功能表达及影响:体外和计算机联合分析

BioImpacts : BI Pub Date : 2019-03-08 DOI: 10.15171/bi.2019.19

M. Asgharzadeh, M. M. Pourseif, J. Barar, M. Eskandani, Mojtaba Jafari Niya, M. Mashayekhi, Y. Omidi

{"title":"Functional expression and impact of testis-specific gene antigen 10 in breast cancer: a combined in vitro and in silico analysis","authors":"M. Asgharzadeh, M. M. Pourseif, J. Barar, M. Eskandani, Mojtaba Jafari Niya, M. Mashayekhi, Y. Omidi","doi":"10.15171/bi.2019.19","DOIUrl":"https://doi.org/10.15171/bi.2019.19","url":null,"abstract":"Introduction: Testis-specific gene antigen 10 (TSGA10) is a less-known gene, which is involved in the vague biological paths of different cancers. Here, we investigated the TSGA10 expression using different concentrations of glucose under hypoxia and also its interaction with the hypoxia-inducible factor 1 (HIF-1). Methods: The breast cancer MDA-MB-231 and MCF-7 cells were cultured with different concentrations of glucose (5.5, 11.0 and 25.0 mM) under normoxia/hypoxia for 24, 48, and 72 hours and examined for the HIF-1α expression and cell migration by Western blotting and scratch assays. The qPCR was employed to analyze the expression of TSGA10. Three-dimensional (3D) structure and the energy minimization of the interacting domain of TSGA10 were performed by MODELLER v9.17 and Swiss-PDB viewer v4.1.0/UCSF Chimera v1.11. The UCSF Chimera v1.13.1 and Hex 6.0 were used for the molecular docking simulation. The Cytoscape v3.7.1 and STRING v11.0 were used for protein-protein interaction (PPI) network analysis. The HIF-1a related hypoxia pathways were obtained from BioModels database and reconstructed in CellDesigner v4.4.2. Results: The increased expression of TSGA10 was found to be significantly associated with the reduced metastasis in the MDA-MB-231 cells, while an inverse relationship was seen between the TSGA10 mRNA level and cellular migration but not in the MCF-7 cells. The C-terminal domain of TSGA10 interacted with HIF-1α with high affinity, resulting in PPI network with 10 key nodes (HIF-1α, VEGFA, HSP90AA1, AKT1, ARNT, TP53, TSGA10, VHL, JUN, and EGFR). Conclusions: Collectively, TSGA10 functional expression alters under the hyper-/hypo-glycemia and hypoxia, which indicates its importance as a candidate bio-target for the cancer therapy.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124294198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Chondroitin sulfate degradation and eicosanoid metabolism pathways are impaired in focal segmental glomerulosclerosis: Experimental confirmation of an in silico prediction 硫酸软骨素降解和类二十烷代谢途径在局局节段性肾小球硬化中受损:计算机预测的实验证实

BioImpacts : BI Pub Date : 2019-03-08 DOI: 10.15171/bi.2019.12

S. Kalantari, M. Naji, M. Nafar, Hootan Yazdani-Kachooei, N. Borumandnia, M. Parvin

{"title":"Chondroitin sulfate degradation and eicosanoid metabolism pathways are impaired in focal segmental glomerulosclerosis: Experimental confirmation of an in silico prediction","authors":"S. Kalantari, M. Naji, M. Nafar, Hootan Yazdani-Kachooei, N. Borumandnia, M. Parvin","doi":"10.15171/bi.2019.12","DOIUrl":"https://doi.org/10.15171/bi.2019.12","url":null,"abstract":"Introduction: Focal segmental glomerulosclerosis (FSGS), the most common primary glomerular disease, is a diverse clinical entity that occurs after podocyte injury. Although numerous studies have suggested molecular pathways responsible for the development of FSGS, many still remain unknown about its pathogenic mechanisms. Two important pathways were predicted as candidates for the pathogenesis of FSGS in our previous in silico analysis, whom we aim to confirm experimentally in the present study. Methods: The expression levels of 4 enzyme genes that are representative of \"chondroitin sulfate degradation\" and \"eicosanoid metabolism\" pathways were investigated in the urinary sediments of biopsy-proven FSGS patients and healthy subjects using real-time polymerase chain reaction (RT-PCR). These target genes were arylsulfatase, hexosaminidase, cyclooxygenase-2 (COX-2), and prostaglandin I2 synthase. The patients were sub-divided into 2 groups based on the range of proteinuria and glomerular filtration rate and were compared for variation in the expression of target genes. Correlation of target genes with clinical and pathological characteristics of the disease was calculated and receiver operating characteristic (ROC) analysis was performed. Results: A combined panel of arylsulfatase, hexosaminidase, and COX-2 improved the diagnosis of FSGS by 76%. Hexosaminidase was correlated with the level of proteinuria, while COX-2 was correlated with interstitial inflammation and serum creatinine level in the disease group. Conclusion: Our data supported the implication of these target genes and pathways in the pathogenesis of FSGS. In addition, these genes can be considered as non-invasive biomarkers for FSGS.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129906766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Numerical investigation of hemodynamic performance of a stent in the main branch of a coronary artery bifurcation 冠状动脉主干支架血流动力学性能的数值研究

BioImpacts : BI Pub Date : 2019-03-08 DOI: 10.15171/bi.2019.13

S. Razavi, V. Farhangmehr, Z. Babaie

{"title":"Numerical investigation of hemodynamic performance of a stent in the main branch of a coronary artery bifurcation","authors":"S. Razavi, V. Farhangmehr, Z. Babaie","doi":"10.15171/bi.2019.13","DOIUrl":"https://doi.org/10.15171/bi.2019.13","url":null,"abstract":"Introduction: The effect of a bare-metal stent on the hemodynamics in the main branch of a coronary artery bifurcation with a particular type of stenosis was numerically investigated by the computational fluid dynamics (CFD). Methods: Three-dimensional idealized geometry of bifurcation was constructed in Catia modelling commercial software package. The Newtonian blood flow was assumed to be incompressible and laminar. CFD was utilized to calculate the shear stress and blood pressure distributions on the wall of main branch. In order to do the numerical simulations, a commercial software package named as COMSOL Multiphysics 5.3 was employed. Two types of stent , namely, one-part stent and two-part stent were applied to prevent the build-up and progression of the atherosclerotic plaques in the main branch. Results: A particular type of stenosis in the main branch was considered in this research. It occurred before and after the side branch. Moreover, it was found that the main branch with an inserted one-part stent had the smallest region with the wall shear stress (WSS) below 0.5 Pa which was the minimum WSS in the main branch without the stenosis. Conclusion: The use of a one-part stent in the main branch of a coronary artery bifurcation for the aforementioned type of stenosis is recommended.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"69 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127275971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

A multi-method and structure-based in silico vaccine designing against Echinococcus granulosus through investigating enolase protein 基于烯醇化酶蛋白的颗粒棘球绦虫硅疫苗设计

BioImpacts : BI Pub Date : 2019-03-08 DOI: 10.15171/bi.2019.18

M. M. Pourseif, M. Yousefpour, M. Aminianfar, G. Moghaddam, A. Nematollahi

{"title":"A multi-method and structure-based in silico vaccine designing against Echinococcus granulosus through investigating enolase protein","authors":"M. M. Pourseif, M. Yousefpour, M. Aminianfar, G. Moghaddam, A. Nematollahi","doi":"10.15171/bi.2019.18","DOIUrl":"https://doi.org/10.15171/bi.2019.18","url":null,"abstract":"Introduction: Hydatid disease is a ubiquitous parasitic zoonotic disease, which causes different medical, economic and serious public health problems in some parts of the world. The causal organism is a multi-stage parasite named Echinococcus granulosus whose life cycle is dependent on two types of mammalian hosts viz definitive and intermediate hosts. Methods: In this study, enolase, as a key functional enzyme in the metabolism of E. granulosus (EgEnolase), was targeted through a comprehensive in silico modeling analysis and designing a host-specific multi-epitope vaccine. Three-dimensional (3D) structure of enolase was modeled using MODELLER v9.18 software. The B-cell epitopes (BEs) were predicted based on the multi-method approach and via some authentic online predictors. ClusPro v2.0 server was used for docking-based T-helper epitope prediction. The 3D structure of the vaccine was modeled using the RaptorX server. The designed vaccine was evaluated for its immunogenicity, physicochemical properties, and allergenicity. The codon optimization of the vaccine sequence was performed based on the codon usage table of E. coli K12. Finally, the energy minimization and molecular docking were implemented for simulating the vaccine binding affinity to the TLR-2 and TLR-4 and the complex stability. Results: The designed multi-epitope vaccine was found to induce anti-EgEnolase immunity which may have the potential to prevent the survival and proliferation of E. granulosus into the definitive host. Conclusion: Based on the results, this step-by-step immunoinformatics approach could be considered as a rational platform for designing vaccines against such multi-stage parasites. Furthermore, it is proposed that this multi-epitope vaccine is served as a promising preventive anti-echinococcosis agent.","PeriodicalId":375065,"journal":{"name":"BioImpacts : BI","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128287913","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 33

Recent advances in targeted therapy of colorectal cancer: impacts of monoclonal antibodies nanoconjugates 单克隆抗体纳米偶联物对结直肠癌靶向治疗的影响

BioImpacts : BI Pub Date : 2019-03-08 DOI: 10.15171/bi.2019.16

Mostafa Akbarzadeh Khiavi, Azam Safary, M. Somi

引用次数: 19