Protistology最新文献_第3页

Niche partitioning within an insect host: trypanosomatids Wallacemonas raviniae and Trypanosoma (Megatrypanum) sp. in the horsefly Hybomitra solstitialis 昆虫寄主内的生态位划分:沙锥虫和大锥虫在冬至马蝇中的分布

Protistology Pub Date : 2022-01-01 DOI: 10.21685/1680-0826-2022-16-2-3

M. N. Malysheva, A. Kostygov, A. Frolov

{"title":"Niche partitioning within an insect host: trypanosomatids Wallacemonas raviniae and Trypanosoma (Megatrypanum) sp. in the horsefly Hybomitra solstitialis","authors":"M. N. Malysheva, A. Kostygov, A. Frolov","doi":"10.21685/1680-0826-2022-16-2-3","DOIUrl":"https://doi.org/10.21685/1680-0826-2022-16-2-3","url":null,"abstract":"Tabanids (horseflies and deerflies) represent the main vectors of Trypanosoma theileri species complex and are frequently infected by them. In these insects, the trypanosomes are transiently present in the midgut and develop predominantly in the ileum. During a survey of infections in tabanids, we encountered a horsefly, in which trypanosomatids were present not only in the ileum but also in the rectum. The analysis of 18S rRNA sequences of the parasites in both locations demonstrated that they represented a T. theileri -like trypanosome and the monoxenous species Wallacemonas raviniae previously described from a flesh fly, respectively. The investigation using light and transmission electron microscopy showed that the two parasites differed not only in their affinity to distinct hindgut sections, but also in the patterns of attachment to the cuticular lining, interaction between individual cells, and development of extracellular structures. Unlike most monoxenous trypanosomatids inhabiting the rectum, W. raviniae was not observed on rectal glands or in the close proximity to them indicating either a peculiarity of the parasite species or specific conditions in the insect host. Our results demonstrated that the two trypanosomatid species partitioned their niches within the horsefly host due to strikingly dissimilar life strategies.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67892187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Morphology and molecular phylogeny of Euplotes japonicum sp. n. (Ciliophora, Euplotidae) from the Peter the Great Gulf, Sea of Japan 日本海彼得大湾的日本纵蝗属(纤毛虫科，纵蝗科)的形态和分子系统发育

Protistology Pub Date : 2022-01-01 DOI: 10.21685/1680-0826-2022-16-4-2

M. Tribun

引用次数: 0

Amoebozoan barcoding marker cytochrome c oxidase (Cox1), RNA editing and issues in creating a public reference sequence database 阿米巴原虫条形码标记细胞色素c氧化酶(Cox1)， RNA编辑和建立公共参考序列数据库的问题

Protistology Pub Date : 2022-01-01 DOI: 10.21685/1680-0826-2022-16-3-8

A. Kudryavtsev

{"title":"Amoebozoan barcoding marker cytochrome c oxidase (Cox1), RNA editing and issues in creating a public reference sequence database","authors":"A. Kudryavtsev","doi":"10.21685/1680-0826-2022-16-3-8","DOIUrl":"https://doi.org/10.21685/1680-0826-2022-16-3-8","url":null,"abstract":"Summary DNA barcoding using a fragment of the mitochondrial cytochrome c oxidase subunit 1 (Cox1) gene is a promising tool not only in animals but also for many groups of protists, including Amoebozoa. To use this tool, we need a reference database for the comparison and assignment of newly obtained sequences. As NCBI/GeneBank® is the most complete molecular sequence database to date, it is logical to use it as a reference database. In fact, it is used as such, when the newly obtained sequences are checked against this database using BLAST. Yet, a quarter of all available barcoding Cox1 sequences of Amoebozoa would not be seen in the BLAST results, as they are deposited with the status ‘UNVERIFIED’. Some of these sequences show reading frame shifts due to multiple single nucleotide deletions. These deletions, seen at the genomic level, may indicate presence of insertional RNA editing in this gene. This phenomenon was experimentally proven only in myxomycetes and Arcellinida among Amoebozoa. Interestingly, many sequences marked as UNVERIFIED do not show frame shifts or other signs of RNA editing, while some of the sequences that are not assigned this status, do. For the sequence database to be fully searchable, new sequences have to be properly accessioned. A recent communication with NCBI confirms that when a sequence has putative editing sites, the submitter should provide a note on this feature and references to appropriate papers. In this case, the sequence can be accessioned normally.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67894556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re-investigation of Vannella ebro Smirnov, 2001 and description of V. navicula sp. nov. (Amoebozoa, Vannellida): further insights into diversity and distribution of marine vannellids Vannella ebro Smirnov, 2001的再调查和V. navicula sp. nov.的描述:对海洋vannellia多样性和分布的进一步认识

Protistology Pub Date : 2022-01-01 DOI: 10.21685/1680-0826-2022-16-4-4

A. Kudryavtsev, E. Volkova, A. Smirnov

引用次数: 0

Description of a new Neoparamoeba aestuarina (Amoebozoa, Dactylopodida) strain from benthic biotopes of the White Sea (Northwestern Russia) 白海(俄罗斯西北部)底栖生物群落中一新种新拟阿米巴(阿米巴目，趾足目)的描述

Protistology Pub Date : 2022-01-01 DOI: 10.21685/1680-0826-2022-16-3-6

E. Volkova, F. Voytinsky

引用次数: 0

Morphology, morphometry and infraciliature of four new species of Clevelandellida de Puytorac and Grain, 1976 (Ciliophora: Armophorea) from the digestive tube of hydromorphic earthworms in the Cameroonian coastal zone 喀麦隆海岸带水形蚯蚓消化管中四新种的形态、形态计量学和基础结构(纤毛虫目:腋窝目)

Protistology Pub Date : 2022-01-01 DOI: 10.21685/1680-0826-2022-16-3-4

Paul Alain Nana, Nectaire Lié Nyamsi Tchatcho, Fils Mamert Onana, Z. Fokam, C. S. Metsopkeng, P. Ngassam, T. Ewoukem, E. Masseret, M. Nola, T. Sime-Ngando

引用次数: 0

Irma Viktorovna Issi (1930-2022) Irma Viktorovna Issi（1930-2022）

Protistology Pub Date : 2022-01-01 DOI: 10.21685/1680-0826-2022-16-4-6

Y. Sokolova, E. Nassonova, Yuri S. Tokarev

引用次数: 0

Vannella salarenaria sp. nov. (Amoebozoa, Vannellida) and its implications for the distribution of amoebae salarenaria sp11 .(变形虫目，变形虫门)及其对变形虫分布的启示

Protistology Pub Date : 2022-01-01 DOI: 10.21685/1680-0826-2022-16-4-3

A. Kudryavtsev, E. Volkova, A. Smirnov

引用次数: 0

A puzzling finding: the Brazilian tomato parasite Phytomonas serpens in the western conifer seed bug Leptoglossus occidentalis in Crimea 一个令人困惑的发现:在克里米亚西部针叶树种子虫Leptoglossus occidentalis的巴西番茄寄生虫Phytomonas serpens

Protistology Pub Date : 2022-01-01 DOI: 10.21685/1680-0826-2022-16-1-4

A. I. Ganyukova, M. N. Malysheva, L. Chistyakova, A. Frolov

引用次数: 0

Molecular detection of microsporidia Vairimorpha ceranae and Nosema bombycis growth in the lepidopteran Sf9 cell line 鳞翅目Sf9细胞中变异小孢子虫和家蚕小孢子虫生长的分子检测

Protistology Pub Date : 2022-01-01 DOI: 10.21685/1680-0826-2022-16-1-3

V. Dolgikh, I. Senderskiy, V. Zhuravlyov, Anastasija N. Ignatieva, S. Timofeev, Diloram A. Ismatullaeva, B. A. Mirzakhodjaev

{"title":"Molecular detection of microsporidia Vairimorpha ceranae and Nosema bombycis growth in the lepidopteran Sf9 cell line","authors":"V. Dolgikh, I. Senderskiy, V. Zhuravlyov, Anastasija N. Ignatieva, S. Timofeev, Diloram A. Ismatullaeva, B. A. Mirzakhodjaev","doi":"10.21685/1680-0826-2022-16-1-3","DOIUrl":"https://doi.org/10.21685/1680-0826-2022-16-1-3","url":null,"abstract":"The search for new therapeutics and strategies to suppress microsporidiosis of domesticated insects requires cultivation of honeybee and silkworm parasites in available insect cell cultures as well as reliable methods of their detection. In this study, we infected the commonly used lepidopteran Sf9 cell line with the Vairimorpha ceranae and Nosema bombycis spores to evaluate molecular methods for microsporidia growth assay. The silkworm parasite N. bombycis effectively develops in lepidopteran cells and, according to literature data, its growth can be detected by qPCR analysis of β -tubulin gene copies in infected Sf9 cultures. Here, we used Western blotting with antibodies against N. bombycis β -tubulin to analyze Sf9 cultures infected with the parasite spores and demonstrated the prospects of immunochemical methods to assay its intracellular growth. Analysis of five genes of N. bombycis spore wall and polar tube proteins in infected cultures by reverse transcription (RT) PCR showed that expression of the polar tube protein PTP2 may serve as a specific marker of the parasite growth because only its transcripts were not detected in freshly inoculated Sf9 cells. The honeybee parasite V. ceranae infects Sf9 cells less efficiently. To find a sensitive and specific marker of the growth of this parasite, we analyzed the transcripts of its 13 genes in infected cultures using the same RT-PCR method. The spore wall protein SWP32 gene demonstrated the highest expression at the 4 th -day post infection with V. ceranae spores, alongside with specificity of PCR-amplification, and the absence of transcripts in freshly inoculated cultures. Thus, quantitative PCR analysis of its expression may help to assay the V. ceranae intracellular growth in the Sf9 cell line.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67892573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3