Applied In Vitro Toxicology最新文献

{"title":"Effects of Subcytotoxic Exposure of Silver Nanoparticles on Osteogenic Differentiation of Human Bone Marrow Stem Cells","authors":"K NguyenAlexander, PatelReema, M NobleJade, ZhengJiwen, J NarayanRoger, KumarGirish, L GoeringPeter","doi":"10.1089/AIVT.2019.0001","DOIUrl":"https://doi.org/10.1089/AIVT.2019.0001","url":null,"abstract":"Abstract Introduction: In vitro toxicology evaluations utilizing human stem cell models represent attractive alternatives to conventional animal models, which are not always predictive of human res...","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/AIVT.2019.0001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46259252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspective on Current Alternatives in Nanotoxicology Research","authors":"Todd A Stueckle, Jenny R. Roberts","doi":"10.1089/aivt.2019.29020.jrr","DOIUrl":"https://doi.org/10.1089/aivt.2019.29020.jrr","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/aivt.2019.29020.jrr","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44078098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Integrative Proteomic/Lipidomic Analysis of the Gold Nanoparticle Biocorona in Healthy and Obese Conditions.","authors":"Lisa M Kobos,&nbsp;Saeed Alqatani,&nbsp;Christina R Ferreira,&nbsp;Uma K Aryal,&nbsp;Victoria Hedrick,&nbsp;Tiago J P Sobreira,&nbsp;Jonathan H Shannahan","doi":"10.1089/aivt.2019.0005","DOIUrl":"https://doi.org/10.1089/aivt.2019.0005","url":null,"abstract":"<p><p><i><b>Introduction:</b></i> When nanoparticles (NPs) enter a physiological environment, a coating of biomolecules or biocorona (BC) forms on the surface. Formation of the NP-BC is dependent on NP properties, the physiological environment, and time. The BC influences NP properties and biological interactions such as cellular internalization, immune responses, biodistribution, and others, leading to pharmacological and toxicological consequences. To date, examination of the NP-BC has focused primarily on protein components and healthy conditions. Therefore, we evaluated the protein and lipid content of BCs that formed on physicochemically distinct gold nanoparticles (AuNPs) under healthy and obese conditions. A comprehensive understanding of the NP-BC is necessary for the translation of <i>in vitro</i> toxicity assessments to clinical applications. <i><b>Materials and Methods:</b></i> AuNPs with two coatings (poly-N-vinylpyrrolidone [PVP] or citrate) and diameters (20 or 100 nm) were incubated in pooled human serum, and an integrated proteomic/lipidomic approach was used to evaluate BC composition. Macrophages were utilized to evaluate differential immune responses due to variations in the AuNP-BC. <i><b>Results:</b></i> AuNPs form distinct BCs based on physicochemical properties and the surrounding environment, with the obese BC containing more proteins and fewer lipids than the healthy BC. Differential macrophage inflammatory responses were observed based on AuNP properties and BC composition. <i><b>Discussion and Conclusion:</b></i> Overall, these findings demonstrate that AuNP size and coating, as well as physiological environment, influence the protein and lipid composition of the BC, which impacts cellular responses following exposure. These findings demonstrate that incorporation of BCs representing distinct physiological conditions may enhance the translatability of nanosafety <i>in vitro</i> studies.</p>","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"5 3","pages":"150-166"},"PeriodicalIF":0.0,"publicationDate":"2019-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/aivt.2019.0005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37836151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of a NovelIn SituHybridization Technology on 3D Organotypic Cell Cultures","authors":"L. Neau, C. Lorin, S. Frentzel, J. Hoeng, A. Iskandar, P. Leroy, Keyur C Trivedi","doi":"10.1089/AIVT.2018.0021","DOIUrl":"https://doi.org/10.1089/AIVT.2018.0021","url":null,"abstract":"Abstract Introduction: Developed by Advanced Cell Diagnostics, RNAscope® in situ hybridization technology enables detection of a target RNA in a cell-specific manner on formalin-fixed paraffin-embe...","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/AIVT.2018.0021","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42995660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Building In Silico Models to Trigger Retesting: A Strategy on How to Use Predictive Models to Identify Potentially Incorrect In Vitro Intrinsic Clearance Results","authors":"SteinmetzFabian Pitter, PeterssonCarl, ZanelliUgo, CzodrowskiPaul","doi":"10.1089/AIVT.2018.0018","DOIUrl":"https://doi.org/10.1089/AIVT.2018.0018","url":null,"abstract":"Abstract Introduction: The results from biological assays, such as microsomal intrinsic clearance, are often associated with moderate to high variability. Nevertheless, it is crucial to disciplines...","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/AIVT.2018.0018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42066978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neurovascular Organotypic Culture Models Using Induced Pluripotent Stem Cells to Assess Adverse Chemical Exposure Outcomes.","authors":"Eric H Nguyen,&nbsp;Micah J Dombroe,&nbsp;Debra L Fisk,&nbsp;William T Daly,&nbsp;Christine M Sorenson,&nbsp;William L Murphy,&nbsp;Nader Sheibani","doi":"10.1089/aivt.2018.0025","DOIUrl":"https://doi.org/10.1089/aivt.2018.0025","url":null,"abstract":"<p><p><b><i>Introduction:</i></b> Human-induced pluripotent stem cells (iPSCs) represent a promising cell source for the construction of organotypic culture models for chemical toxicity screening and characterization. <b><i>Materials and Methods:</i></b> To characterize the effects of chemical exposure on the human neurovasculature, we constructed neurovascular unit (NVU) models consisting of endothelial cells (ECs) and astrocytes (ACs) derived from human-iPSCs, as well as human brain-derived pericytes (PCs). The cells were cocultured on synthetic poly(ethylene glycol) (PEG) hydrogels that guided the self-assembly of capillary-like vascular networks. High-content epifluorescence microscopy evaluated dose-dependent changes to multiple aspects of NVU morphology. <b><i>Results:</i></b> Cultured vascular networks underwent quantifiable morphological changes when incubated with vascular disrupting chemicals. The activity of predicted vascular disrupting chemicals from a panel of 38 compounds (U.S. Environmental Protection Agency) was ranked based on morphological features detected in the NVU model. In addition, unique morphological neurovascular disruption signatures were detected per chemical. A comparison of PEG-based NVU and Matrigel^™-based NVU models found greater sensitivity and consistency in chemical detection by the PEG-based NVU models. <b><i>Discussion:</i></b> We suspect that specific morphological changes may be used for discerning adverse outcome pathways initiated by chemical exposure and rapid mechanistic characterization of chemical exposure to neurovascular function. <b><i>Conclusion:</i></b> The use of human stem cell-derived vascular tissue and PEG hydrogels in the construction of NVU models leads to rapid detection of adverse chemical effects on neurovascular stability. The use of multiple cell types in coculture elucidates potential mechanisms of action by chemicals applied to the model.</p>","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":"5 2","pages":"92-110"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/aivt.2018.0025","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37836150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profibrotic Activity of Multiwalled Carbon Nanotubes Upon Prolonged Exposures in Different Human Lung Cell Types","authors":"S. Chortarea, Fikad Zerimariam, H. Barosova, D. Septiadi, M. J. Clift, A. Petri‐Fink, B. Rothen‐Rutishauser","doi":"10.1089/AIVT.2017.0033","DOIUrl":"https://doi.org/10.1089/AIVT.2017.0033","url":null,"abstract":"Abstract Introduction: The expanding use of multiwalled carbon nanotubes (MWCNTs) in various consumer and industrial products has raised concerns regarding the potential health risks following the ...","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/AIVT.2017.0033","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45382506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Evaluation of Potential Inhibition of CYP3A4/5 and CYP2C9 Enzymatic Activity by Boswellia serrata Extract","authors":"L RoeAmy, WilcoxRachel, M PriceJason, LiLijuan, DaiHong, M FreemanKimberly, W FrileyWeslyn, G HermanAshley, B BlackChristopher, R BrouwerKenneth, P JacksonJonathan","doi":"10.1089/AIVT.2018.0023","DOIUrl":"https://doi.org/10.1089/AIVT.2018.0023","url":null,"abstract":"Abstract Introduction: As the use of dietary supplements increases, botanical–drug interaction (BDI) potentials should be evaluated. Boswellia serrata extract (BSE) is traditionally used as an anti...","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/AIVT.2018.0023","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48150096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenone, Hydroxybenzophenone, and Branched Phenone ER Binding and Vitellogenin Agonism in Rainbow Trout <i>In Vitro</i> Models.","authors":"Mark A Tapper, Jeffrey S Denny, Jose A Serrano, Richard C Kolanczyk, Barbara R Sheedy, Grace M Overend, Michael W Hornung, Patricia K Schmieder","doi":"10.1089/aivt.2018.0008","DOIUrl":"10.1089/aivt.2018.0008","url":null,"abstract":"<p><p>Phenones and hydroxy benzophenones are widely used as UV radiation filters, and in the manufacturing of insecticides and pharmaceuticals. Understanding the estrogenic potential these chemicals is of interest to the US Environmental Protection Agency and other international environmental organizations. The current study sequentially combined complementary <i>in vitro</i> rainbow trout estrogen receptor (rtER) binding and liver slice vitellogenin (Vtg) mRNA induction assays in the context of a defined ER-mediated adverse outcome pathway (AOP). Cyclic phenones, branched phenones, and hydroxybenzophenones bound to rtER with relative potency ranging from no affinity to high binding affinity of 0.11%, and many induced Vtg gene expression in rt liver slices. In addition, cyclohexylphenylketone which did not bind rtER binding in cytosol was biotransformed within liver tissue to a chemical that induced Vtg expression.</p>","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":"62-74"},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11370886/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45904048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspective on the Use of Alternative Methods for Assessing the Safety of Botanicals","authors":"A. Roe","doi":"10.1089/AIVT.2019.29019.ALR","DOIUrl":"https://doi.org/10.1089/AIVT.2019.29019.ALR","url":null,"abstract":"","PeriodicalId":37448,"journal":{"name":"Applied In Vitro Toxicology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1089/AIVT.2019.29019.ALR","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42969112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}