Vy Nguyen Hoang Thuy, T. Thanh, Binh Nguyen Quoc, H. Kieu, Duong Van, Thao Luu Thi Thach, Vu Thanh Nguyen
{"title":"Generation of Transgenic Medaka Oryzias curvinotus (Nichols & Pope, 1927) Carrying a Cyan Fluorescent Protein Gene Driven by Alpha Actin Promoter","authors":"Vy Nguyen Hoang Thuy, T. Thanh, Binh Nguyen Quoc, H. Kieu, Duong Van, Thao Luu Thi Thach, Vu Thanh Nguyen","doi":"10.33997/J.AFS.2021.34.1.006","DOIUrl":"https://doi.org/10.33997/J.AFS.2021.34.1.006","url":null,"abstract":"The study aimed to produce fluorescent protein transgenic medaka Oryzias curvinotus (Nichols & Pope, 1927) as a novel strain of ornamental fish. These fish were produced by transferring a plasmid consisting of a fluorescent reporter gene and a strong promoter into one-cell stage embryos. For this purpose, myosin light chain 2, but not other promoters, was mainly used. The study also evaluated the stability of the transgenic medaka germline acquiring vivid fluorescent phenotypes via the transgenesis of the cyan fluorescent protein (CFP) gene under the control of O. curvinotus skeletal alpha-actin (OCacta) promoter. The pOCacta-CFP plasmid, containing a OCacta promoter and CFP reporter gene, was transferred into the one-cell stage of O. curvinotus embryos by a microinjection technique. As a result, 36 of 1386 microinjected O. curvinotus embryos exhibited CFP signals in their trunks. The expressed CFP signals in O. curvinotus embryos and adults were detected under a microscope using a green fluorescent protein (GFP) filter (450–490 nm wavelength), and blue LED light (400–450 nm wavelength). Five O. curvinotus founders showing clear CFP signals were selected and crossed with non-transgenic counterparts to produce subsequent generations. Among strains, the frequency of germline transmission from founder to F1 was highly variable. Only two of the five founders successfully pass the transgene to the F1 generation. At present, the progeny of subsequent generations is being produced and tested for the expression of CFP signals, and therefore, stable lines are ongoing.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47629961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Siti Norita Mohamad, W. N. Noordin, Noor Faizah Ismail, A. Hamzah
{"title":"Red Hybrid Tilapia (Oreochromis spp.) Broodstock Development Programme in Malaysia: Status, Challenges and Prospects for Future Development","authors":"Siti Norita Mohamad, W. N. Noordin, Noor Faizah Ismail, A. Hamzah","doi":"10.33997/J.AFS.2021.34.1.008","DOIUrl":"https://doi.org/10.33997/J.AFS.2021.34.1.008","url":null,"abstract":"The red hybrid tilapia (Oreochromis spp.) and the black tilapia, including the genetically improved farmed tilapia (GIFT) (Oreochromis niloticus (Linnaeus, 1758)) collectively contributed to about 30.7 % of the total freshwater aquaculture production in Malaysia in 2018. The red hybrid tilapia is the most important, accounting for 94 % of total tilapia production. Among the major problems encountered in tilapia farming is the inconsistent supply of quality and adequate seeds, which could be solved through systematic breeding programmes. Among the major problems encountered in tilapia species farming is the inconsistent supply of quality and adequate seeds, which could be solved through systematic breeding programmes. This paper discusses the status of the red hybrid tilapia breeding programme in Malaysia, including issues and future perspectives. In brief, the first systematic breeding programme was initiated by the Fisheries Research Institute (FRI) in 2008. Selected founder stocks from Malaysia, Taiwan and Thailand were used to establish a base population for the programme. In this programme, the combined selection was practised which produced six generations of selection and successfully improved 12.5 % of harvest body weight per generation. The 6th generation was used as one of the founder stocks, apart from FRI Glami Lemi, Negeri Sembilan and Pahang populations to improve resistance to Streptococcus agalactiae. In 2017, the scope of the programme was expanded to include molecular tool in identifying markers for growth. Ten SNP markers associated with high growth performance traits were discovered. The provision of better breeding stocks for the aquaculture industry and the development of safe and productive operations are expected to result in more stable fish production and an improved income for farmers.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41341796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Relationship Between Size at Maturity and Maximum Size in Cichlid Populations Corroborates the Gill-Oxygen Limitation Theory (GOLT)","authors":"Upali Sarath Amarasinghe, D. Pauly","doi":"10.33997/J.AFS.2021.34.1.002","DOIUrl":"https://doi.org/10.33997/J.AFS.2021.34.1.002","url":null,"abstract":"Fish generally mature at a smaller fraction of their maximum sizes than birds and mammals. The farmed tilapia (Family Cichlidae) can tolerate adverse conditions that result in stunting and which also cause the fish to spawn at small size. Such spawning at small size (or ‘early spawning’) is usually perceived as a unique feature of tilapia. The mechanism that explains how stressful environmental conditions tend to reduce the maximum size that fish can reach is very general and should apply to all fish. However, not all fish species are equally hardy, and most fish do not survive in the stunted or dwarf form under stressful environmental conditions. Tilapia, and other cichlids, on the other hand, can handle stressful conditions, if by remaining stunted. The present study shows that tilapia and other cichlids do not spawn ‘earlier’ than other teleosts. Rather, they are exceptionally tolerant of stressful environmental conditions, but with elevated metabolism. By reducing their growth and the maximum size they can reach ‘stunting’, they also reduce the sizes at which their maturity is initiated (‘early spawning’). This corroborates the gill-oxygen limitation theory (GOLT), which identifies spawning as an event rather than a determinant of fish growth.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46611046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Beyond Bycatch: The Species Diversity of Tonguesole (Pleuronectiformes: Cynoglossidae) in Coastal Fisheries of the Tanintharyi Region, Southern Myanmar","authors":"I. Segura-García, Sabai Soe, N. Tun, S. Box","doi":"10.33997/J.AFS.2021.34.1.003","DOIUrl":"https://doi.org/10.33997/J.AFS.2021.34.1.003","url":null,"abstract":"Flatfishes in the family Cynoglossidae are an important coastal fishery in Myanmar. Due to the overlapping morphologies of multiple tonguesole species, caught both as bycatch from trawl fisheries and targeted specifically by small scale fishers, they are all marketed under a single local name, “khwayshar”. This presents a management challenge given the potential differences in the species-specific life-histories, population dynamics, fishing vulnerability and harvest rates. This study investigated the species diversity of tonguesole landings from coastal communities of the Tanintharyi Region of southern Myanmar. DNA barcoding was used to distinguish potentially 10 different species, of which five were identified to species level and five at the genus level. Unconfirmed genetic identifications were based on external morphology. The poor efficacy of DNA barcoding for tonguesole species identification resulted from the limited DNA barcode reference sequences available for the family Cynoglossidae in public databases. An asymmetric occurrence and relative abundance of the identified species in landing sites where samples were collected suggested that the most common species was Cynoglossus oligolepis (Bleeker, 1855), a new species record for Myanmar, followed by Cynoglossus lingua Hamilton, 1822. The results of the present study provide new information to characterise the tonguesole fishery as a first step in the development of management plans for the coastal fishery in Myanmar.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42611734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
T. Khuu, Thi Ngoc Hoa Nguyen, Nguyen Hai Tai Tran, Yoko Saito, T. Matsuishi
{"title":"How Much Do Farmers Expect to Implement for Traceability? Evidence From a Double-Bound Choices Experiment of Vietnamese Shrimp Aquaculture","authors":"T. Khuu, Thi Ngoc Hoa Nguyen, Nguyen Hai Tai Tran, Yoko Saito, T. Matsuishi","doi":"10.33997/J.AFS.2021.34.1.009","DOIUrl":"https://doi.org/10.33997/J.AFS.2021.34.1.009","url":null,"abstract":"Traceability is considered the most important requirement for shrimp products exported to global markets. However, implementing traceability in shrimp-exporting countries is challenging because of limited production at the local supply chain and lack of financial welfare awareness. This study aims to investigate the expected farm-gate price for traceability implementation using a double-bound dichotomous choice experiment. The censored regression model is used to estimate the factors influencing the anticipated farm-gate price of shrimp farmers. The survey was conducted in Ca Mau Province, Vietnam, by interviewing 71 Penaeus monodon Fabricius, 1798, and 43 Penaeus vannamei Boone, 1931, farmers. To implement traceability, P. monodon farmers estimated the farm-gate price at 10.17 USD.kg-1 , while P. vannamei farmers expected 6.18 USD.kg-1 . Application of international quality assurance certifications, willingness to implement traceability, land used, culture methods, shrimp species, current farm-gate price, and variable costs affected the expected farm-gate price. The attractive anticipated farm-gate price compensated for the negative influence of applying international quality assurance certifications, indicating that the farmers were willing to implement traceability. This suggests that the application of certifications increased the ability to implement traceability in the shrimp supply chain. The attractive farm-gate price for certified shrimp products would enhance their willingness to implement the traceability of shrimp products.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48772144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Baliga, Puneeth Thadooru Goolappa, M. Shekar, S. K. Girisha, K. S. Ramesh, Vilasini Udyavara, M. N. Venugopal
{"title":"Bacterial Communities Associated With Penaeus vannamei Boone, 1931 Surface and Its Rearing Water in Biofloc Culture System","authors":"P. Baliga, Puneeth Thadooru Goolappa, M. Shekar, S. K. Girisha, K. S. Ramesh, Vilasini Udyavara, M. N. Venugopal","doi":"10.33997/J.AFS.2021.34.1.004","DOIUrl":"https://doi.org/10.33997/J.AFS.2021.34.1.004","url":null,"abstract":"The biofloc system is an ecologically sustainable shrimp culture system. The conglomerates of beneficial bacteria, algae and protozoa in pond water serve as a water quality management system and as a feed additive to the shrimps. This study aimed to characterise the microbial communities associated with the biofloc pond water and the surface of Penaeus vannamei Boone, 1931, reared in it using the Illumina Miseq sequencing technology. The multiple alpha diversity measures indicated the shrimp surface samples to be richer in diversity than the pond water samples. Analysis of the bacterial community revealed that Proteobacteria, Bacteroidetes, Planctomycetes and Cyanobacteria formed the principal phyla. There was a shift in the relative abundance of bacterial communities at each time point. The operational taxonomic units (OTU) analyses revealed that 18.38 % OTUs were shared by the pond water samples, the shrimp surface samples shared 29.35 % at the three different time points. PICRUST analysis revealed that the bacterial communities in the biofloc rearing water, and shrimp surface, were likely involved in intensive microbial metabolism and core housekeeping functions. The information generated will help understand the bacterial community composition associated with optimal water quality and shrimp health in a biofloc culture system.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45925778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Suresh, B. Nair, Arya Unni, Binumon Thankachan Mangalathettu
{"title":"Histopathology of Anilocra leptosoma Bleeker, 1857 (Isopoda, Cymothoidae) Infestation on Its New Host Nematalosa nasus (Bloch, 1795) From India","authors":"A. Suresh, B. Nair, Arya Unni, Binumon Thankachan Mangalathettu","doi":"10.33997/J.AFS.2021.34.1.010","DOIUrl":"https://doi.org/10.33997/J.AFS.2021.34.1.010","url":null,"abstract":"Cymothoid isopods are parasitic crustaceans that cause serious impact on marine fish and might lead to fish mortality and consequently, economic losses. Histopathological alterations caused by Anilocra spp. have not been studied well. This study aims to report the histopathological changes caused by Cymothoid, Anilocra leptosoma Bleeker, 1857 in the skin of Bloch's gizzard shad, Nematalosa nasus (Bloch, 1795). Histopathological examination of processed skin tissues showed changes caused by A. leptosoma, such as hyperplasia and erosions of the epidermis associated dermal oedema and muscle degeneration. The host response also included an aggregation of subepithelial dense sheets of hemosiderin-laden macrophages within the dense mixed inflammatory cells. The cymothoid, A. leptosoma are serious parasites of marine fish that can cause severe economic loss in the commercially important fish species. The present study represents the first record of the parasitic cymothoid, A. leptosoma on N. nasus from India.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43211686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Extent, Dynamics, and Potential Predictors of Technical Efficiency and Capacity Utilisation in Small-Scale Fisheries in Oman","authors":"M. Al-Siyabi, S. Bose, H. Al-Masroori","doi":"10.33997/J.AFS.2021.34.1.007","DOIUrl":"https://doi.org/10.33997/J.AFS.2021.34.1.007","url":null,"abstract":"This paper investigates the extent, dynamics, and factors influencing technical efficiency (TE) and capacity utilisation (CU) in small-scale fisheries (SSF) using a two-stage data envelopment analysis (DEA) approach covering the period 2010–2012. A considerable extent of boat-level technical inefficiency, capacity underutilisation and scale inefficiency were evident. On average, TE and CU levels under the constant returns to scale (CRS) and variable returns to scale (VRS) models declined over time. The TE and CU scores of 2010 remained unaltered with the addition of ‘fishing time’ as an input to the model. The proportion of boats with unitary scale efficiency (SE) decreased from 26 % in 2010 to 12 % in 2012. The underutilisation rates of the inputs ‘crew’ and ‘fishing time’ ranged from 15.5 % to 31.6 % and 15.8 % to 28.6 %, respectively. Among the species category, the extent of excess capacity was 70 % to 156 % and 47 % to 119 % under the CRS and VRS models, respectively. The second-stage DEA results indicated that the explanatory variables ‘fishing location’, ‘catch per unit of effort’ (CPUE), ‘fuel costs’ and ‘crew share’ significantly influenced CU under the CRS model. In contrast, the significant influence of subsidies and other operating costs were noted under the VRS model. For the TE case, ‘age’, ‘education’, ‘subsidy’ and ‘CPUE’ were found to be significant under the CRS and VRS models. Other significant variables were found in the study under CRS and VRS models. Finally, the results from the descriptive and empirical analysis under the two-stage DEA model are discussed together with policy implications.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48458836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Price Premium and Resource Rents from Nile Tilapia Oreochromis niloticus (Linnaeus, 1758) Farming in Lake Sebu, Philippines","authors":"Rosalina Palanca-Tan","doi":"10.33997/j.afs.2020.33.4.006","DOIUrl":"https://doi.org/10.33997/j.afs.2020.33.4.006","url":null,"abstract":"With deep lakes and cool weather, high-quality tilapia Oreochromis niloticus (Linnaeus, 1758) is produced in the Municipality of Lake Sebu in the southern Philippines, fetching a price premium that results in Ricardian resource rent for fish cage operators. This extra rent has induced unsustainable aquaculture practices such as overcrowding fish cages, overstocking and overfeeding, which in turn, have resulted in water pollution and fish kills that partially wipe out the rent. This paper aims to estimate sustainable tilapia production and potential resource rent from tilapia farming in Lake Sebu using primary data gathered from key informant interviews, focus group discussions and a survey of tilapia cage owners. Results indicate that potential annual resource rent ranges from PHP49–61 million (USD0.95–1.18 million). Expressed on a per square meter of fish cage area, rent is calculated to be PHP12.22–15.21 or USD0.24–0.29, 8–10 times more than the annual fish cage permit fee of PHP1.5 (USD0.03). This Ricardian rent may be collected to fund the necessary lake water quality rehabilitation programs and aquaculture monitoring and regulation enforcement activities to prevent rent-dissipating fish kills and to ensure the preservation of the natural fisheries capital. Rent collection may be in the form of an additional permit fee that follows a progressive rate structure to address poverty and equity issues. The study illustrates the multi-faceted role of resource rent in fisheries exploitation and management, and adds to the still scarce literature on resource rent estimation in fisheries.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45026765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Species Composition, Abundance and Conservation Status of Some Economically Important Macrobenthic Invertebrates In Pag-asa Island, Kalayaan, Palawan, Philippines","authors":"R. A. Balisco","doi":"10.33997/j.afs.2020.33.4.007","DOIUrl":"https://doi.org/10.33997/j.afs.2020.33.4.007","url":null,"abstract":"Pag-asa Island is the largest and the only civilian inhabited island in the Kalayaan Island Group (KIG) in the West Philippine Sea. The waters around these islands are important fishing grounds; however, little information is available on the status of macrobenthic invertebrates within the vicinity of the island. In this study, we assessed the species composition, abundance and conservation status of some commercially important macrobenthic invertebrates in shallow reefs and seagrass beds surrounding the island. The sampling was conducted during day time of dry season for 6 days (May 9-14, 2018) through wading, snorkelling and scuba diving which recorded a total of seven species, mostly bivalves and echinoderms. Bivalve species included the giant clams Tridacna maxima (Röding, 1798) and Tridacna crocea Lamarck, 1819; echinoderms included Bohadschia argus Jaeger, 1833 , Bohadschia vitiensis (Semper, 1868) , Holothuria atra Jaeger, 1833 , Holothuria leucospilota (Brandt, 1835) and Tripneustes gratilla (Linnaeus, 1758). These species occurred more abundantly in seagrass beds than in coral reefs, ranging from 46.7–1,346.7 ind.ha -1 . Most of the species were of “Least Concern” status according to International Union for Conservation of Nature (IUCN) Red List. The absence of previously reported high valued species in the surveyed sites, such as topshell Rochia nilotica (Linnaeus, 1767) and lobsters Panulirus versicolor (Latreille, 1804) could be an effect of unregulated harvesting. Effective resource management in this part of KIG is needed to allow the recovery of reduced populations and ensure food security for the inhabitants of the island.","PeriodicalId":37296,"journal":{"name":"Asian Fisheries Science","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46238376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}