Decellularized Extracellular Matrix最新文献_第2页

CHAPTER 16. Fibroblastic Cell-derived Extracellular Matrices: A Cell Culturing System to Model Key Aspects of the Tumor Microenvironment 第十六章。纤维母细胞衍生的细胞外基质:一个模拟肿瘤微环境关键方面的细胞培养系统

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00305

J. Gardiner, K. Raghavan, J. Alexander, J. Franco-Barraza, E. Cukierman

引用次数: 4

CHAPTER 14. Decellularized Cell-Secreted Matrices: Novel Materials for Superior Stem Cell Expansion and Tissue Engineering 第14章。脱细胞细胞分泌基质:用于高级干细胞扩增和组织工程的新材料

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00259

Michael C. Yang, B. Kalionis, D. Heath

引用次数: 1

CHAPTER 13. Engineering an Endocrine Neo-Pancreas 第13章。工程内分泌新胰腺

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00237

Eriselda Keshi, I. Sauer, K. Hillebrandt

{"title":"CHAPTER 13. Engineering an Endocrine Neo-Pancreas","authors":"Eriselda Keshi, I. Sauer, K. Hillebrandt","doi":"10.1039/9781788015998-00237","DOIUrl":"https://doi.org/10.1039/9781788015998-00237","url":null,"abstract":"The severe short- and long-term unwanted effects and the huge economic burden of diabetes mellitus have led to immense research efforts regarding possible therapeutic modalities. Subcutaneous insulin injection and external insulin pumps, the so-called open-loop methods, albeit already established in clinical practice, are unable to maintain long-term normoglycemia. The most novel therapeutic options, summarized under the term closed-loop models, comprise pancreas transplantation, islet cell transplantation and artificial endocrine pancreas devices. Even though very promising, various obstacles hinder the translation of these concepts to the bedside. The fact that the disease only affects the endocrine pancreas and the already assembled insights into extracellular matrix and the effects its components exert on cell growth, proliferation and functionality encouraged abundant research on bioengineering an endocrine extracellular matrix-based implantable organ. Indeed, whole-organ decellularization and the subsequent perfusion recellularization of the scaffold with endothelial and islet cells has generated a new treatment alternative capable of eliminating many downsides of the exiting options. Nevertheless, further research is needed to widen our understanding of the extracellular matrix, its immunogenicity and the fundamental components on which cellular livelihood and proliferation depends, in order to achieve long-term in vitro and in vivo results.","PeriodicalId":370951,"journal":{"name":"Decellularized Extracellular Matrix","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127360959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHAPTER 8. Decellularized Extracellular Matrix as Bioink for 3D-Bioprinting 第八章。脱细胞细胞外基质作为3d生物打印的生物链接

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00139

Sanskrita Das, A. Mukhtar, Jinah Jang, J. Shim

{"title":"CHAPTER 8. Decellularized Extracellular Matrix as Bioink for 3D-Bioprinting","authors":"Sanskrita Das, A. Mukhtar, Jinah Jang, J. Shim","doi":"10.1039/9781788015998-00139","DOIUrl":"https://doi.org/10.1039/9781788015998-00139","url":null,"abstract":"Decellularized extracellular matrix (dECM) encompasses a complex milieu of proteins, glycosaminoglycans and other essential growth factors providing the native microenvironment to the cells. Together with the superiority of dECM to comprehensively mimic the native tissue microenvironment, 3D bioprinting offers a promising and alternative platform to fabricate engineered constructs in recreating functional tissue models. Tissue specific 3D bioprinted constructs emerge as the most biomimetic module as they provide unique biochemical cues and biophysical signals to regulate cell–cell and cell–ECM interaction. In this chapter, we introduce the 3D bioprinting techniques and the necessary requirements for printing using an extrusion-based 3D bioprinter. In addition, we also discuss the advantage of using tissue specific bioink over homogenous polymer and briefly summarize the different decellularization methods involved in obtaining dECM bioink while preserving the ECM components. Finally, we highlight the studies based on 3D bioprinting of tissue-specific dECM bioink and its flexibility to fabricate cell-laden tissue constructs for wide range of applications, such as tissue regeneration and drug screening. The chapter ends with concluding remarks as well as the future directions of using dECM bioink with regard to animal choice, improvement in its mechanical properties and also the commercialization of the 3D printed tissue-engineered constructs.","PeriodicalId":370951,"journal":{"name":"Decellularized Extracellular Matrix","volume":"410 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125758435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHAPTER 6. Immune Responses to Decellularized Matrices 第六章。对脱细胞基质的免疫反应

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00095

Alexandra H. Scanameo, N. Ziats

引用次数: 1

CHAPTER 2. Preparation Methods for Tissue/Organ-derived dECMs – Effects on Cell Removal and ECM Changes 第二章。组织/器官来源的decm制备方法-对细胞去除和ECM变化的影响

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00015

T. Yamaoka

引用次数: 1

CHAPTER 7. Decellularized Extracellular Matrix Hydrogels: Fabrication, Properties, Characterization, and Current Applications 第七章。脱细胞细胞外基质水凝胶:制备、性质、表征和当前应用

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00116

Pamela Duran, M. Alperin, K. Christman

引用次数: 1