Decellularized Extracellular Matrix最新文献

CHAPTER 10. Use of Small Intestinal Submucosa dECM in Tissue Engineering and Regenerative Medicine 第十章。小肠黏膜下层dECM在组织工程和再生医学中的应用

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00179

J. Hodde

引用次数: 1

CHAPTER 9. Mechanical Property Tunable dECM and Their Regenerative Applications 第9章。机械性能可调dECM及其再生应用

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00163

M. Hwang, Kwideok Park

{"title":"CHAPTER 9. Mechanical Property Tunable dECM and Their Regenerative Applications","authors":"M. Hwang, Kwideok Park","doi":"10.1039/9781788015998-00163","DOIUrl":"https://doi.org/10.1039/9781788015998-00163","url":null,"abstract":"The extracellular matrix (ECM) provides both structural support and biochemical and biophysical cues through a complex and highly-organized conglomerate of macromolecules. Such roles have led to interest in harnessing ECM for various regenerative applications. To capture the full potential of ECM, the various biophysical and biochemical cues of ECM must be fully defined and decoded. Frequently used platforms employ coatings of a single ECM protein on synthetic substrates. While such platforms have undeniably led to advancements in the field it is important to recognize that they are based on a reductionist approach in that the natural microenvironment is resolved into a single variable (e.g. stiffness, roughness, topography, etc.). In this chapter, organ, tissue, and cell-derived ECM – collectively called decellularized ECM (dECM) –are taken comprehensively, in which an entire spectrum of biophysical variables are integrated into one platform. Given that stiffness plays a large role in determining biological responses, we assess different chemical, biological, and physical methods to modulate the stiffness, and subsequently the global biophysical profile, of dECM. We also examine the use of such stiffness-tuned dECM platforms for various regenerative applications. Ultimately, the use of stiffness-tuned dECM should provide valuable information parallel to that obtained through traditional means such as synthetic substrates.","PeriodicalId":370951,"journal":{"name":"Decellularized Extracellular Matrix","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131986564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHAPTER 4. Bared Basement Membrane Substrata: Design, Cellular Assembly, Decellularization and Application to Tissue Regeneration and Stem Cell Differentiation 第四章。裸基膜基质:设计、细胞组装、脱细胞及其在组织再生和干细胞分化中的应用

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00051

K. Mochitate, Reiko Nagano, Yukiko Toya-Nakajima

{"title":"CHAPTER 4. Bared Basement Membrane Substrata: Design, Cellular Assembly, Decellularization and Application to Tissue Regeneration and Stem Cell Differentiation","authors":"K. Mochitate, Reiko Nagano, Yukiko Toya-Nakajima","doi":"10.1039/9781788015998-00051","DOIUrl":"https://doi.org/10.1039/9781788015998-00051","url":null,"abstract":"Basement membrane (BM) is a compact and continuous sheet of 50–350 nm thickness and located underneath epithelium, endothelium, etc. It helps tissue repair and morphogenesis, and stem cells' differentiation to mature phenotypes. We have devised several culture models for cellular assembly of BM in vitro and developed a thorough removal of the covering BM-forming cells in order to utilize the bared BM as a culture substratum. Thus de novo synthesized BM (sBM) substrata of LN-511 isoform normally guided tissue progenitors or embryonic stem cells to terminal differentiation and morphologically and functionally mature phenotypes, for example, airway ciliated cells, hepatocytes and pancreatic β cells. Therefore sBM has been proven to be a matrix, that is, solid-phase cradle. BM formation, which we introduce in this chapter, has the advantages of it not being necessary to prepare each BM component, but also being free from clearing irregular aggregates that come from malassembly among them. We simply culture epithelial and endothelial cells etc. on the fibrillar collagen substratum that has been coated beforehand with N-acetylglucosamine ligands-branching or -sprouting hydrophobic polymer, and wait for automatic assembly of BM structure by these cells. rLN-10 cells, genetically modified cells, are advantageous for forming an optimized sBM.","PeriodicalId":370951,"journal":{"name":"Decellularized Extracellular Matrix","volume":"138 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114452910","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHAPTER 12. Decellularized Matrix for Corneal Tissue Engineering: Recent Advances in Development and Clinical Potential 第十二章。角膜组织工程脱细胞基质研究进展及临床应用前景

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00219

Y. Hashimoto, S. Funamoto, S. Sasaki, T. Kimura, Hisatoshi Kobayashi, A. Kishida

{"title":"CHAPTER 12. Decellularized Matrix for Corneal Tissue Engineering: Recent Advances in Development and Clinical Potential","authors":"Y. Hashimoto, S. Funamoto, S. Sasaki, T. Kimura, Hisatoshi Kobayashi, A. Kishida","doi":"10.1039/9781788015998-00219","DOIUrl":"https://doi.org/10.1039/9781788015998-00219","url":null,"abstract":"The worldwide shortage of donated human corneas for transplantation is a serious problem. Induced pluripotent stem (iPS) cells have yielded new possibilities for regenerating the corneal epithelium and endothelium, which consist only of cells. However, regeneration of the corneal stroma, which accounts for approximately 90% of the entire corneal thickness, has not yet achieved fully satisfactory results despite much effort. This might be because the corneal stroma possesses a highly ordered, stacked hierarchical structure, which contributes to the maintenance of corneal transparency as well as its mechanical properties. Currently, the decellularized cornea has received considerable attention for use as an artificial corneal stroma. The decellularized cornea is an extracellular matrix (ECM) from which cellular components and other immunogens have been removed, but which maintains the structural and mechanical integrity of the corneal stroma. This chapter summarizes decellularization techniques for the preparation of decellularized corneal matrices, the clinical potential of xenotransplantation with grafts of decellularized corneal matrices, and the recent advances in and limitations of commercially available decellularized corneal matrices. Finally, we summarize research on the decellularized cornea and discuss future perspectives to realize fully functional bio-engineered corneas to replace donated human corneas.","PeriodicalId":370951,"journal":{"name":"Decellularized Extracellular Matrix","volume":"52 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123648393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CHAPTER 3. Preparation of Cultured Cell-derived Decellularized Matrix (dECM) – Factors Influencing dECM Formation and Its Ability 第三章。细胞源脱细胞基质的制备——影响dECM形成的因素及其能力

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00029

T. Hoshiba, N. Kawazoe, G. Chen

引用次数: 0

CHAPTER 11. Small-diameter Acellular Vascular Grafts: From Basic Research to Clinical Application 第十一章。小直径脱细胞血管移植:从基础研究到临床应用

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00199

A. Mahara, T. Yamaoka

引用次数: 0

CHAPTER 1. Extracellular Matrix Scaffolds for Tissue Engineering and Biological Research 第1章。细胞外基质支架用于组织工程和生物学研究

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00001

T. Hoshiba, T. Yamaoka

引用次数: 7

CHAPTER 17. Extracellular Matrix in Peripheral Nerve Regeneration 第十七章。细胞外基质在周围神经再生中的应用

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00328

Sheng Yi, Lingchi Xu, Yumin Yang, X. Gu

引用次数: 0

CHAPTER 15. Decellularized Extracellular Matrix for the Regulation of Stem Cell Differentiation 第15章。脱细胞细胞外基质对干细胞分化的调控作用

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00286

T. Hoshiba

引用次数: 0

CHAPTER 5. A Novel Treatment for Giant Congenital Melanocytic Nevi Combining Inactivated Nevus Tissue by Pressurization and Cultured Epidermal Autograft 第五章。灭活痣组织加压与自体培养表皮移植联合治疗先天性巨大黑素细胞痣的新方法

Decellularized Extracellular Matrix Pub Date : 2019-12-05 DOI: 10.1039/9781788015998-00077

N. Morimoto, A. Mahara, T. Yamaoka

{"title":"CHAPTER 5. A Novel Treatment for Giant Congenital Melanocytic Nevi Combining Inactivated Nevus Tissue by Pressurization and Cultured Epidermal Autograft","authors":"N. Morimoto, A. Mahara, T. Yamaoka","doi":"10.1039/9781788015998-00077","DOIUrl":"https://doi.org/10.1039/9781788015998-00077","url":null,"abstract":"Giant congenital melanocytic nevi (GCMN) are intractable skin tumors associated with a risk of melanoma. Reconstructing such large, full-thickness skin defects after removing nevi using skin grafting is difficult. To overcome these issues, cultured epidermal autograft (CEA) was approved for the treatment of GCMN patients in 2016 in Japan. However, the take rate of CEA to wound beds lacking dermis is unsatisfactory. We started reconstructing the autologous dermis from the removed GCMN itself. Using high hydrostatic pressure (HHP), we prepared the dermal layer using the removed inactivated nevus tissue. Human skin and human nevus could be inactivated by HHP at 200 MPa without any detectable damage to the dermal structure or epidermal basement membrane. Cultured epidermis (CE) took to skin and nevus inactivated by HHP at 200 MPa without removing cellular remnants. One problem is the inflammation caused by the remaining cellar remnants, so we are evaluating the inflammation in an ongoing clinical trial. If the efficacy and safety of this strategy for retransplanting autologous tumor tissue as the autologous native matrix can be validated in a clinical trial, it could be widely applied to other tissues, such as bone, cartilage, nerve or vessels, that are usually discarded after tumor removal.","PeriodicalId":370951,"journal":{"name":"Decellularized Extracellular Matrix","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132580703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0