Applied Environmental Biotechnology最新文献

{"title":"Micro- and nano-plastics in hydroponic environment are critical for plants: A meta-analysis","authors":"Shi-peng Li, Yu-Xi Feng, Xiao-Zhang Yu","doi":"10.26789/aeb.2023.01.002","DOIUrl":"https://doi.org/10.26789/aeb.2023.01.002","url":null,"abstract":"The presence of microplastics (MPs) and nanoplastics (NPs) in the environment is ubiquitous, and as such, the toxicity of these plastics, exposure scenarios, and mechanisms of plant response is to be determined. Hereby, a meta-analysis is performed to investigate the effects of MPs and/or NPs on different plant species under hydroponics and soil conditions to assess the current scenario. We examined the response level of root system, photosynthetic parameters, and antioxidant system of plants against MPs/NPs. Root response level in soil condition against various concentrations and types of MPs was higher than in hydroponics however, this response was opposite for the types of MPs. Photosynthetic parameters, including chlorophyll a and b, carotenoids, total chlorophyll, and maximum quantum efficiency of PSII were higher in soil conditions than in hydroponics. Antioxidant parameters, such as malondialdehyde (MDA) and H2O2 contents were higher in hydroponics plants, while, superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) showed a mix trend of response level. In addition, proline content was significantly higher in soil and ascorbic acid (ASA) in hydroponic cultured plants under different types, sizes, and concentrations of MPs. These three systems i.e., root, photosynthesis, and antioxidant parameters were also compared between different species, however, the results are generally consistent with the above mentions one. Overall, these analyses suggest that plants grown in hydroponics are more sensitive to the plastic pollution than in the soil environment.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69127227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of Spirulina (Arthrospira platensis) as a microbial diatory feed supplement for juvenile of Nile Tilapia (Oreochromis niloticus)","authors":"Hafidh Al Mahrouqi, Sergey Dobretsov, Roberto Tefilo Abdala-Daz","doi":"10.26789/aeb.2023.01.001","DOIUrl":"https://doi.org/10.26789/aeb.2023.01.001","url":null,"abstract":"Tilapia is the second most farmed fish in tropical and sub-tropical countries worldwide. This study investigated the effect of replacing fish meal by Spirulina platensis (groups fed with 5%, 10% and 15% Spirulina) on the growth of Nile tilapia O. niloticus juveniles. Intestinal microbiota of tilapia has been studied by MiSeq Illumina sequencing. No significant differences (P > 0.05) were observed for the protein, carbohydrate and lipids of fish fed with control food and different concentrations of spirulina. However, the Margalef species richness of the intestinal bacteria of tilapia fed with spirulina was significantly higher (P < 0.05) compared to the control samples. Evenness was significantly higher (P < 0.05) for tilapia fed with food substituted with the highest concentration of spirulina. Also, few potentially probiotic genera, like Bacillus and Actinomycetes were detected in the gut of the experimented Tilapia. On the other hand, the highest percent of potentially pathogenic genera was recorded for the genera Pseudomonas and Corynebacterium. In most cases, the highest abundances of potentially pathogenic species were found in control samples. As manipulation of the feed was shown to improve Tilapia microbiota, which could lead to further improvements in aquaculture production.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69127218","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Popularization or Population of Science and the Future","authors":"Ji-Dong Gu","doi":"10.26789/aeb.2022.01.001","DOIUrl":"https://doi.org/10.26789/aeb.2022.01.001","url":null,"abstract":"Science is experiencing a different model for a wider dissemination with the widely accessibility of internet today at the information age and high connectivity. This technological development has changed the daily life of peoples globally and has been mainly appraised with positive effects, but very little, if any, negative ones are being discussed, specifically on fundamental matters. In this article I would like to present the emerging negativities and potential detrimental effects on science and its advances to be made. Popularization and population are two words chosen and used here to describe the way in which dissemination of available and accessible scientific information is being made. The new Open Access model of publication and its apparent dominance in the academic publishing market by commercial companies for publication of scientific papers are passing the threshold point and eroding the quality of science in my view. Scientific societies as the most important groups to the establishment of academic standards and to keep new science discoveries to be checked are increasingly forced to cope with the commercial publication practices in terms of speeding up the reviewing and publishing more and quickly, competing for the publishing market and profit to be made, and most importantly, compromising the academic standards. When profit is the major driving force for the publishers, including scientific societies, quality of science in academic papers is yielded to the financial interest and profit, and the purposes of publishing are not for science and its development as the goal. The role of scientific publication in our society must be checked by relevant administration of the governments and national academies together with the scientific societies so that the quality of academic publication of scientific results in science can be maintained at high confident level to serve the society in a positive manner into the future. Commercial publication is another avenue to allow more scientific results to be published in additional to journals by societies, but the relationship between them must be a quality-based judgement and decision, not a pure business competition for success to rule this market.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43857658","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The removal of antibiotic resistance genes by different biological treatment processes of four wastewater treatment plants in various seasons","authors":"Zimu Wang, Guolan Wang, Jieting Qu, Wentao Hong, L. Luo","doi":"10.26789/aeb.2022.01.005","DOIUrl":"https://doi.org/10.26789/aeb.2022.01.005","url":null,"abstract":"The widespread antibiotic resistance genes (ARGs) severely jeopardizing human health have been widely concerned. Wastewater treatment plants (WWTPs) are commonly considered the important sources and sinks of ARGs due to their roles in depositing ARGs and provoking ARGs' proliferation. Current WWTPs often use biological treatment to remove pollutants, whereas the purification efficacy of ARGs is unclear. Thus, this study investigated the seasonal changes of ARGs (tetA, tetC, tetM, tetO, tetQ, tetW, and tetX, as representative) and a class of integron (intI1) in the sludge of different treatment units of four WWTPs to understand the role of WWPTs in ARGs spread. It is found that ARGs abundance varied between summer and winter depending on the types of ARGs and treatment units. The cumulative abundance of ARGs in the same treatment unit showed no obvious difference between seasons. By comparison, ARGs abundance was generally lower in the anoxic unit than in the oxic unit. Moreover, the WWTPs with Cyclic Activated Sludge System (CASS) process reduced the amount of ARGs in sludge, while the WWTPs with Anaerobic-Anoxic-Oxic (A2O) increased ARGs abundance. In conclusion, this study demonstrated that the excess sludge still posed a risk for ARG spread after biological treatment.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69126501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sequencing or not sequencing","authors":"J. Gu","doi":"10.26789/aeb.2022.02.001","DOIUrl":"https://doi.org/10.26789/aeb.2022.02.001","url":null,"abstract":"Technology has been playing a very important role in the advancement of science, and big breakthroughs in science are dependent upon technologies available from time to time. Between science and technology, a good understanding is required so that available technologies can be used most efficiently and effectively to solving the targeted questions in scientific research. Genome nucleotide sequencing as an available technology after several generations of the technical improvement is capable of extract the full nucleotide sequences of any single organism or a mixed population of organisms in a given sample. This technology has a major important role in medicine and health science for diagnosis of the microorganisms and early detection of genetic diseases. In microbial ecology and applied microbiology, it is now fashionable to sequence the microbial community, the microbiome, so that the community composition and diversity are described from air, soil, water, sludge to skin and gut of animals, including humans. This routine practice, too frequently used by far too many, neither provide any significant insights of knowledge nor unravel the fundamental science in any way, but it serves as a cosmetic decoration for publication mostly. Because of this, not sequencing or sequencing less is the view expressed here to alert researchers for a better focus on the research questions, and the selection and implementation of the necessary physical science methods to obtain the solid research data that can advance science in a significant way. Publication driven experiments to produce more papers are eroding the essence of science, especially the pure spirit of science.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69126555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and selection of salt-tolerant bacterial strains capable of solubilizing phosphorus and synthesizing phosphatase enzyme from rice-shrimp soil in Mekong River Delta, Vietnam","authors":"Nguyen Khoi Nghia, Nguyen Thi Kieu Oanh, Nguyen Hoai Thanh, Le Thi Xa, Duong Minh Vien, Chau Thi Anh Thy","doi":"10.26789/aeb.2022.02.002","DOIUrl":"https://doi.org/10.26789/aeb.2022.02.002","url":null,"abstract":"The aim of this study was to isolate and select salt tolerant bacteria having both functions in phosphorus solubilization and phosphatase synthesis from rice-shrimp farming soil in saline areas in Mekong River Delta of Vietnam. Phosphorus solubilizing bacteria were isolated on National Botanical Research Institute's Phosphate (NBRIP) agar medium containing 1% NaCl and the activity of phosphatase enzyme was determined by disodium p-nitrophenyl phosphate reagent method at a wavelength of 420 nm. The result showed that from 15 saline soil samples, a total of 95 strains of phosphorus solubilizing bacteria were isolated and 19 of them showed their good phosphorus solubilization. The results about phosphatase activities of these 19 strains illustrated that TBT5-3 bacterial strain was the highest phosphatase producing strain with an amount of 0.377 U/mL after 10 days of incubation. This strain showed its best phosphatase producing capacity when cultured in the liquid culture medium containing pH 5, 1% NaCl, glucose and urea under the shaking speed of 120 rpm. Based on 16S rRNA gene sequence analysis this phosphatase synthesizing bacterial strain was genetically identified as species of Bacillus sp. TBT5-3 since 100% of this train sequence is affiliated with Bacillus megaterium.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69126642","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recycling of biogas wastewater to organic fertilizers and influence of organic fertilizers on maize, mung bean growth and yield under the field conditions","authors":"Nguyen Khoi Nghia, Chau Thi Anh Thy, Le Thi Xa, Do Thanh Luan","doi":"10.26789/aeb.2022.01.003","DOIUrl":"https://doi.org/10.26789/aeb.2022.01.003","url":null,"abstract":"The objective of the study was to utilize the biogas effluents to produce solid and liquid organic fertilizers, as well as evaluate their effect on the growth and yield of corn and mung beans under field conditions. For liquid organic fertilizer, biogas effluents were mixed with fish emulsion and beneficial bacteria while biogas effluents-absorbing coal slag was mixed with sugarcane filter, fishmeal and beneficial bacteria to create solid organic fertilizer. Liquid organic fertilizer was irrigated with a dose of 5 L/1000 m2 while solid organic fertilizer was applied with a dose of 1 ton/ha with 75% recommended NPK formula for maize and mung bean. The results showed that applying solid or liquid organic fertilizer formulated from biogas effluents with other amendments helped to reduce the amount of recommended NPK fertilizer by 25%, but still maintained growth and yield of maize and mungbean equivalent to the control treatment fertilized with 100% recommended NPK. In conclusion, the amount of organic matter and N, P, K in biogas effluents from biogas digesters can be utilized to produce organic fertilizers which not only help to reduce chemical fertilizers, but also solve the environmental problems and create new friendly value-added products for practicing sustainable agricultural production.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69126398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soil fungi, Aspergillus niger NAAC, as environmental pollution clean-up agent against Progesterone: Remediation strategy and preparation of Bioformulation","authors":"A. Chatterjee, Nida Afreen","doi":"10.26789/aeb.2022.02.003","DOIUrl":"https://doi.org/10.26789/aeb.2022.02.003","url":null,"abstract":"The presence of hormones, drugs and chemicals in the environment are disrupting the ecosystem. The existence of chemicals in the environment is a threat to the ecosystem as they have dangerous effects on animals, plants and microbes. Progesterones are steroid hormones used for human contraceptives and therapeutic purposes as well as promoting animal growth. The cases of consumption of progesterone for medical purposes are much higher than estrogens, however, much studies related to estrogens have been conducted thereby neglecting the effects of progesterone. This invention focuses on removal of progesterone using fungal strain. Aspergillus niger NAAC efficiently degraded the progesterone content and transformed it into non-toxic end product. The uniqueness of this study involves preparation of a formulated product which would store the fungal strain and maintain its viability. The bioformulation was prepared using used vegetable cooking oil mixed with water as carrier. The bioformulation would reduce the efforts required to isolate the microorganism for regular usage and can be commercialized for large scale applications. To determine the storage conditions of bioformulation, various parameters were analysed which showed that storing the formulation in air tight container at room temperature would result in maximum longevity of the efficient strain.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69127143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular characterization of a polycyclic aromatic hydrocarbons (PAHs) degrader, Burkholderia contaminans strain P14, isolated from aged oil-contaminated soil in Kuwait","authors":"A. Akbar, Rita Rahmeh, Mohamad Kishk, Batool Akbar, Mustafa Al-Shamali, D. Al-Baijan","doi":"10.26789/aeb.2022.02.005","DOIUrl":"https://doi.org/10.26789/aeb.2022.02.005","url":null,"abstract":"Soil contamination by polycyclic aromatic hydrocarbons (PAHs) and heavy metals is a major concern affecting soil quality. Bioremediation is an efficient approach to decontaminate these pollutants while posing the lowest risk to the environment. This technique is based on identifying microorganisms with the metabolic potential to degrade the pollutants. In this study, the physiochemical properties of oil-contaminated soils in Kuwait were investigated. The soil had alkaline pH (8.36) with a salinity of 0.08% and PAHs compounds were detected at high concentrations (pyrene, 1212 mg/kg), (phenanthrene, 710 mg/kg) and (fluorene 326 mg/kg). Heavy metals such as iron, aluminum, sodium, magnesium, vanadium, copper, etc., were also detected in the soil samples. Then, selective screening for PAH degraders was performed and assessed by colony forming units (CFU) and 16S rDNA gene sequences to determine their growth profiles and taxonomical identification. In total, 21 strains were selected for their resistance to PAHs, which include Pseudomonas (9), Burkholderia (6), Bacillus (2), Bordetella (1), Microbacterium (1), Micrococcus (1), and Kocuria (1). Among all, the growth by Burkholderia sp. P14, in the presence of phenanthrene and fluorene, has maintained a stationary phase from day 5 to 8 with a 6 log CFU/ml bacterial count. The draft genome of the Burkholderia contaminans P14 strain comprised 68 contigs with 8,584,157bp, 66% GC content, 4 rRNA and 75 tRNA. A total of 80 genes were involved in the degradation of the benzoate, naphthalene, and PAH . Genes that encode the PAH degradation were clustered into four distinct groups, including pcaHG, pcaB, pcaIJ, and pcaKFR and were found to be in contigs 5, 11, 8 and 13 of B. contaminans P14, respectively. KEGG analysis suggested that PAHs were degraded in P14 via the protocatechuate and catechol branches of the β-ketoadipate protocatechuate degradation pathway. The genomic island regions in P14 differed from those in the reference genome of B. contaminans M14, indicating the novelty and genomic recombination of the strain. Genomic information on P14 has helped clarify the molecular mechanisms involved in PAH catabolism. Burkholderia contaminans P14 strain will enhance the bioremediation of oil-contaminated soils.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69127162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study of Antibacterial Activity of Citrus and Jabali Honeys with Manuka Honey","authors":"Mohammad A. Alkafaween, Hidaeyh Kafaween, Rania M. Al-Groom","doi":"10.26789/aeb.2022.01.004","DOIUrl":"https://doi.org/10.26789/aeb.2022.01.004","url":null,"abstract":"Abstract: Honey is a natural product that has been widely used for its therapeutic effects. Honey is effective against pathogenic bacteria in inhibiting planktonic antibiotic sensitive strains and antimicrobial resistant organisms. To date, there is no comparative study of the antibacterial activity of Citrus honey (CH) and Jabali honey (JH) with that of Manuka honey (MH). Therefore, the purpose of this study was to conduct such study and to compare the antibacterial activity of CH and JH with that of MH against Pseudomonas aeruginosa. The antibacterial, antibiofilm and antivirulence activities of CH, JH and MH against P. aeruginosa were investigated by agar well diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), time-kill curve, disruption of microcolony, microtiter plate and reverse transcription-quantitative Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR). Agar inhibition assay showed that CH, JH and MH at 20% has antibacterial activity against P. aeruginosa with an inhibition zone of 14.1±0.1 mm, 12.2±0.2 mm and 10.9±0.1 mm respectively. The results showed that the MICs value of CH and JH was 25% compared to MH (12.5%) and the MBCs value of CH and JH was 50% compared to MH (25%) against P. aeruginosa. In addition, the MIC50 and MIC90 for CH and JH were 25% and 50% respectively compared to MH (MIC50; 12.5% and MIC90; 25%) against P. aeruginosa. All tested honeys were found to disrupt the microcolony formation in P. aeruginosa. In time-kill curve, treatment of P. aeruginosa with 2×MIC (Minimum inhibitory concentration) of MH, CH and JH for 12-hours resulted in reduction in colony-forming unit (CFU/ml). The lowest concentration of 20% of MH, CH and JH was found to inhibit and eradicate biofilm formation. RT-qPCR analysis revealed that the expression of all genes (oprB, oprC, fleN, fleQ, fleR, lasR and lasI) in P. aeruginosa were downregulated after exposure to all tested honeys. Among the all-tested honeys, MH showed the highest total antibacterial, antibiofilm and antivirulence activities. This study indicates that CH and JH has antibacterial and antibiofilm activities compared with MH due to a decrease in expression of essential genes associated with P. aeruginosa.","PeriodicalId":36987,"journal":{"name":"Applied Environmental Biotechnology","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69126476","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}