柑橘、贾巴利蜂蜜与麦卢卡蜂蜜抑菌活性的比较研究

Q2 Agricultural and Biological Sciences
Mohammad A. Alkafaween, Hidaeyh Kafaween, Rania M. Al-Groom
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引用次数: 3

摘要

摘要:蜂蜜是一种天然产物,因其治疗作用而被广泛使用。蜂蜜对病原菌具有抑制浮游抗生素敏感菌和耐药菌的作用。柑橘蜂蜜(CH)和贾巴里蜂蜜(JH)与麦卢卡蜂蜜(MH)的抑菌活性尚未进行比较研究。因此,本研究的目的就是开展这一研究,比较CH和JH与MH对铜绿假单胞菌的抑菌活性。采用琼脂孔扩散、最低抑菌浓度(MIC)、最低杀菌浓度(MBC)、时间杀伤曲线、微菌落破坏、微滴板和逆转录定量实时聚合酶链反应(RT-qPCR)等方法研究了CH、JH和MH对铜绿假单胞菌的抑菌活性、抗菌膜活性和抗毒活性。琼脂抑制实验表明,20%浓度的CH、JH和MH对铜绿假单胞菌的抑菌作用范围分别为14.1±0.1 mm、12.2±0.2 mm和10.9±0.1 mm。结果表明,CH和JH对铜绿假单胞菌的mic值比MH(12.5%)高25%;CH和JH对铜绿假单胞菌的MBCs值比MH(25%)高50%。此外,与MH相比,CH和JH的MIC50和MIC90分别为25%和50% (MIC50;12.5%, MIC90;25%)抗铜绿假单胞菌。所有测试的蜂蜜都被发现破坏铜绿假单胞菌的微菌落形成。在时间杀伤曲线上,MH、CH和JH的2×MIC(最低抑制浓度)作用铜绿假单胞菌12小时后,菌落形成单位(CFU/ml)降低。最低浓度为20%的MH、CH和JH对生物膜的形成有抑制和根除作用。RT-qPCR分析显示,暴露于所有蜂蜜后,铜绿假单胞菌中所有基因(oprB、oprC、fleN、fleQ、fleR、lasR和lasI)的表达均下调。在所有蜂蜜中,MH的总抗菌活性、抗菌膜活性和抗毒活性最高。本研究表明,与MH相比,CH和JH具有抗菌和抗生物膜活性,这是由于与铜绿假单胞菌相关的必需基因表达减少所致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Comparative Study of Antibacterial Activity of Citrus and Jabali Honeys with Manuka Honey
Abstract: Honey is a natural product that has been widely used for its therapeutic effects. Honey is effective against pathogenic bacteria in inhibiting planktonic antibiotic sensitive strains and antimicrobial resistant organisms. To date, there is no comparative study of the antibacterial activity of Citrus honey (CH) and Jabali honey (JH) with that of Manuka honey (MH). Therefore, the purpose of this study was to conduct such study and to compare the antibacterial activity of CH and JH with that of MH against Pseudomonas aeruginosa. The antibacterial, antibiofilm and antivirulence activities of CH, JH and MH against P. aeruginosa were investigated by agar well diffusion, Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), time-kill curve, disruption of microcolony, microtiter plate and reverse transcription-quantitative Quantitative Real-Time Polymerase Chain Reaction (RT-qPCR). Agar inhibition assay showed that CH, JH and MH at 20% has antibacterial activity against P. aeruginosa with an inhibition zone of 14.1±0.1 mm, 12.2±0.2 mm and 10.9±0.1 mm respectively. The results showed that the MICs value of CH and JH was 25% compared to MH (12.5%) and the MBCs value of CH and JH was 50% compared to MH (25%) against P. aeruginosa. In addition, the MIC50 and MIC90 for CH and JH were 25% and 50% respectively compared to MH (MIC50; 12.5% and MIC90; 25%) against P. aeruginosa. All tested honeys were found to disrupt the microcolony formation in P. aeruginosa. In time-kill curve, treatment of P. aeruginosa with 2×MIC (Minimum inhibitory concentration) of MH, CH and JH for 12-hours resulted in reduction in colony-forming unit (CFU/ml). The lowest concentration of 20% of MH, CH and JH was found to inhibit and eradicate biofilm formation. RT-qPCR analysis revealed that the expression of all genes (oprB, oprC, fleN, fleQ, fleR, lasR and lasI) in P. aeruginosa were downregulated after exposure to all tested honeys. Among the all-tested honeys, MH showed the highest total antibacterial, antibiofilm and antivirulence activities. This study indicates that CH and JH has antibacterial and antibiofilm activities compared with MH due to a decrease in expression of essential genes associated with P. aeruginosa.
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来源期刊
Applied Environmental Biotechnology
Applied Environmental Biotechnology Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
3.70
自引率
0.00%
发文量
2
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