Nanotheranostics最新文献_第7页

Combination of precipitation and size exclusion chromatography as an effective method for exosome like extracellular vesicle isolation from pericardial fluids. 沉淀法和粒径排除色谱法联合分离心包液外泌体样细胞外泡的有效方法。

Nanotheranostics Pub Date : 2023-01-01 DOI: 10.7150/ntno.82939

Dhananjie Chandrasekera, Rishi Shah, Isabelle van Hout, Willow De Jonge, Richard Bunton, Dominic Parry, Philip Davis, Rajesh Katare

{"title":"Combination of precipitation and size exclusion chromatography as an effective method for exosome like extracellular vesicle isolation from pericardial fluids.","authors":"Dhananjie Chandrasekera, Rishi Shah, Isabelle van Hout, Willow De Jonge, Richard Bunton, Dominic Parry, Philip Davis, Rajesh Katare","doi":"10.7150/ntno.82939","DOIUrl":"https://doi.org/10.7150/ntno.82939","url":null,"abstract":"Extracellular vesicles (EVs), such as exosomes, are nanovesicles that have received significant attention due to their ability to contain various molecular cargos. EVs found in biological fluids have been demonstrated to have therapeutic potential, including as biomarkers. Despite being extensively studied, a significant downfall in EV research is the lack of standardised protocol for its isolation from human biological fluids, where EVs usually exist at low densities. In this study, we tested two well-established EV isolation protocols, precipitation, and size exclusion chromatography (SEC), to determine their efficiency in isolating EVs from the pericardial fluid. Precipitation alone resulted in high yields of low-purity exosomes as tested by DLS analysis, transmission electron microscopy, immunogold labelling and western blotting for the exosomal surface proteins. While EVs isolated by SEC were pure, the concentration was low. Interestingly, the combination of precipitation followed by SEC resulted in high EV yields with good purity. Our results suggest that the combination method can be adapted to isolate EVs from body fluids which have low densities of EV.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"7 4","pages":"345-352"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10161387/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10139909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a Personalised Device for Systemic Magnetic Drug Targeting to Brain Tumours. 开发用于脑肿瘤系统磁性药物靶向的个性化设备。

Nanotheranostics Pub Date : 2023-01-01 DOI: 10.7150/ntno.76559

Priya Patel, Areej Alghamdi, Gary Shaw, Christopher Legge, Maggie Glover, Danielle Freeman, Harry Hodgetts, Erica Wilson, Faith Howard, Sarah Staniland, Aneurin J Kennerley, Duncan Wood, Robert Moorehead, Charlotte Hadfield, Ola Rominiyi, Jon Griffin, Spencer J Collis, Sam Hyde, Marcus Crossley, Martyn Paley, Munitta Muthana

{"title":"Development of a Personalised Device for Systemic Magnetic Drug Targeting to Brain Tumours.","authors":"Priya Patel, Areej Alghamdi, Gary Shaw, Christopher Legge, Maggie Glover, Danielle Freeman, Harry Hodgetts, Erica Wilson, Faith Howard, Sarah Staniland, Aneurin J Kennerley, Duncan Wood, Robert Moorehead, Charlotte Hadfield, Ola Rominiyi, Jon Griffin, Spencer J Collis, Sam Hyde, Marcus Crossley, Martyn Paley, Munitta Muthana","doi":"10.7150/ntno.76559","DOIUrl":"10.7150/ntno.76559","url":null,"abstract":"Delivering therapies to deeply seated brain tumours (BT) is a major clinical challenge. Magnetic drug targeting (MDT) could overcome this by rapidly transporting magnetised drugs directly into BT. We have developed a magnetic device for application in murine BT models using an array of neodymium magnets with a combined strength of 0.7T. In a closed fluidic system, the magnetic device trapped magnetic nanoparticles (MNP) up to distances of 0.8cm. In mice, the magnetic device guided intravenously administered MNP (<50nm) from the circulation into the brain where they localised within mouse BT. Furthermore, MDT of magnetised Temozolomide (TMZmag+) significantly reduced tumour growth and extended mouse survival to 48 days compared to the other treatment groups. Using the same principles, we built a proof of principle scalable magnetic device for human use with a strength of 1.1T. This magnetic device demonstrated trapping of MNP undergoing flow at distances up to 5cm. MDT using our magnetic device provides an opportunity for targeted delivery of magnetised drugs to human BT.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"7 1","pages":"102-116"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760367/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9195277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hybrid Inorganic Complexes as Cancer Therapeutic Agents: In-vitro Validation. 杂化无机配合物作为癌症治疗剂:体外验证。

Nanotheranostics Pub Date : 2023-01-01 DOI: 10.7150/ntno.81557

Murlidhar A Betallu, Shaileshkumar R Bhalara, Kailash B Sapnar, Vijay B Tadke, Keerti Meena, Ananya Srivastava, Gopal C Kundu, Mahadeo Gorain

{"title":"Hybrid Inorganic Complexes as Cancer Therapeutic Agents: In-vitro Validation.","authors":"Murlidhar A Betallu, Shaileshkumar R Bhalara, Kailash B Sapnar, Vijay B Tadke, Keerti Meena, Ananya Srivastava, Gopal C Kundu, Mahadeo Gorain","doi":"10.7150/ntno.81557","DOIUrl":"https://doi.org/10.7150/ntno.81557","url":null,"abstract":"A series of novel mixed transition metal-Magnesium tartarate complexes of general formulation [MMg(C4H4O6)2 .xH2O] (where M = Mn, Fe, Co, Ni, Cu and Zn) is prepared with bidentate tartarate ligand. The synthesized complexes (C1 to C6) are characterized by various analytical techniques such as Elemental analysis, Thermo gravimetric analysis, FT-IR Spectroscopy, X-ray Diffraction, Magnetic susceptibility study etc. All complexes exhibit the composition MMgL2 where M = Mn(II), Fe(II), Co(II), Ni(II), Cu(II) and Zn(II) and L = bidentate tartarate ligand. Analytical data reveals all complexes possesses 1:1 (metal: ligand) ratio. FT-IR spectral study shows that bidentate tartarate ligand coordinate with metal ion in a bidentate manner through two oxygen atoms. Thermo gravimetric analysis of all complexes shows that degradation curves of complexes agrees with recommended formulae of the complexes. X-ray diffraction technique suggests that all complexes (C1 to C6) are polycrystalline in nature. All newly synthesized metal tartarate complexes and ligand were screened in vitro for their anticancer activity against human breast cancer (MDA-MB-231) cell line. The bioassays of all these complexes showed C3 (Co) and C5 (Cu) Mg-tartarate complexes contains maximum antiproliferative activity at 200 µg/ml concentration on MDA-MB-231 cells as compared to other complexes. MDA-MB-231 cells treated with C3 (Co) and C5 (Cu) Mg-tartarate complexes also showed inhibition in cell migration.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"7 3","pages":"270-280"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10093419/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9386899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Ultra-small NIR-Responsive Nanotheranostic Agent for Targeted Photothermal Ablation Induced Damage-Associated Molecular Patterns (DAMPs) from Post-PTT of Tumor Cells Activate Immunogenic Cell Death. 靶向光热消融诱导肿瘤细胞ptt后损伤相关分子模式(DAMPs)激活免疫原性细胞死亡的超小nir反应纳米治疗剂

Nanotheranostics Pub Date : 2023-01-01 DOI: 10.7150/ntno.76720

Shankar Sobhana, Namratha Partha Sarathy, Laxmanan Karthikeyan, Krishnamurthy Shanthi, Raju Vivek

{"title":"Ultra-small NIR-Responsive Nanotheranostic Agent for Targeted Photothermal Ablation Induced Damage-Associated Molecular Patterns (DAMPs) from Post-PTT of Tumor Cells Activate Immunogenic Cell Death.","authors":"Shankar Sobhana, Namratha Partha Sarathy, Laxmanan Karthikeyan, Krishnamurthy Shanthi, Raju Vivek","doi":"10.7150/ntno.76720","DOIUrl":"https://doi.org/10.7150/ntno.76720","url":null,"abstract":"Theranostic nanoparticles (TNPs) is an efficient avenue that culminates both diagnosis and therapy into cancer treatment. Herein, we have formulated a theranostic nanocomposite (NC) with CuS being the ultra-small core component. To ensure stability to the NC, PEI was added which is a vital anchoring group polymer, especially on sulfide surfaces, and adds quality by being a better stabilizer and reducing agent. Additionally, to add stability, specificity, and added photothermal efficiency to the fabricated NC. In addition, encapsulation of indocyanine green (ICG), an efficient NIR absorber, and Folic acid (FA) were conjugated systematically, characterized, and analyzed for photo-stability. The photothermal conversion efficiency of the novel NC (CuS-PEI-ICG-FA) was analyzed at 808 nm, where the NC efficiently converted light energy to heat energy. The NC was also tested for hemocompatibility to clarify and also determined biocompatibility. Surprisingly, damage-associated molecular patterns (DAMPs) from post-PTT of tumor cells activate immunogenic cell death (ICD) for tumor-specific immune responses. The deserving photothermal performance and photo-stability makes the NC an ideal platform for photoacoustic imaging (PAI). A superior contrast was observed for PAI in a concentration-dependent manner enhancing the level of penetration into tissues, thereby better imaging. On account of this study, the newly formulated NC could be utilized as a \"nanotheranostic\" designed for therapeutic and image diagnostic agent of cancer biomedical applications.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"7 1","pages":"41-60"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10701875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Nanofiber scaffolds based on extracellular matrix for articular cartilage engineering: A perspective. 基于细胞外基质的纳米纤维支架在关节软骨工程中的应用前景。

Nanotheranostics Pub Date : 2023-01-01 DOI: 10.7150/ntno.78611

Elham Ahmadian, Aziz Eftekhari, Dawid Janas, Parviz Vahedi

{"title":"Nanofiber scaffolds based on extracellular matrix for articular cartilage engineering: A perspective.","authors":"Elham Ahmadian, Aziz Eftekhari, Dawid Janas, Parviz Vahedi","doi":"10.7150/ntno.78611","DOIUrl":"https://doi.org/10.7150/ntno.78611","url":null,"abstract":"Articular cartilage has a low self-repair capacity due to the lack of vessels and nerves. In recent times, nanofiber scaffolds have been widely used for this purpose. The optimum nanofiber scaffold should stimulate new tissue's growth and mimic the articular cartilage nature. Furthermore, the characteristics of the scaffold should match those of the cellular matrix components of the native tissue to best merge with the target tissue. Therefore, selective modification of prefabricated scaffolds based on the structure of the repaired tissues is commonly conducted to promote restoring the tissue. A thorough analysis is required to find out the architectural features of scaffolds that are essential to make the treatment successful. The current review aims to target this challenge. The article highlights different optimization approaches of nanofibrous scaffolds for improved cartilage tissue engineering. In this context, the influence of the architecture of nanoscaffolds on performance is discussed in detail. Finally, based on the gathered information, a future outlook is provided to catalyze development in this promising field.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"7 1","pages":"61-69"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10701874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 22

A systemic review on development of mesoporous nanoparticles as a vehicle for transdermal drug delivery. 介孔纳米颗粒作为透皮给药载体的开发系统综述。

Nanotheranostics Pub Date : 2023-01-01 DOI: 10.7150/ntno.77395

Praveen Kolimi, Sagar Narala, Ahmed Adel Ali Youssef, Dinesh Nyavanandi, Narendar Dudhipala

{"title":"A systemic review on development of mesoporous nanoparticles as a vehicle for transdermal drug delivery.","authors":"Praveen Kolimi, Sagar Narala, Ahmed Adel Ali Youssef, Dinesh Nyavanandi, Narendar Dudhipala","doi":"10.7150/ntno.77395","DOIUrl":"10.7150/ntno.77395","url":null,"abstract":"Recent advances in drug delivery technologies utilizing a variety of carriers have resulted in a paradigm shift in the current approach to diagnosis and therapy. Mesoporous silica nanoparticles (MSNs) were developed in response to the need for materials with high thermal, chemical, and mechanical properties. The synthesis, ease of surface functionalization, tunable pore size, large surface area, and biocompatibility of MSNs make them useful in a variety of biomedical applications such as drug delivery, theranostics, and stem cell research. In addition, MSNs have a high capability of delivering actives ranging from small molecules such as drugs and amino acids to larger peptides, vaccines, and antibodies in general. Moreover, MSN-based transdermal delivery has sparked a lot of interest because of the increase in drug stability, permeation, and ease of functionalization. The functionalization of MSNs plays an important role in the efficient delivery of therapeutic agents in a highly controlled manner. This review introduced dermal and transdermal drug delivery systems, explained the anatomy of the skin, and summarized different barriers that affect the transdermal delivery of many therapeutic agents. In addition, the fundamentals of MSNs together with their physicochemical properties, synthesis approaches, raw materials used in their fabrication, and factors affecting their physicochemical properties will be covered. Moreover, the applications of MSNs in dermal and transdermal delivery, the biocompatibility of MSNs in terms of toxicity and safety, and biodistribution will be explained with the help of a detailed literature review. The review is covering the current and future perspectives of MSNs in the pharmaceutical field with therapeutic applications.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"7 1","pages":"70-89"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10009011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Rapid, Convergent Approach to the Identification of Exosome Inhibitors in Breast Cancer Models. 一种快速、收敛的方法来鉴定乳腺癌模型中的外泌体抑制剂。

Nanotheranostics Pub Date : 2023-01-01 DOI: 10.7150/ntno.73606

Zoraida Andreu, Esther Masiá, David Charbonnier, María J Vicent

{"title":"A Rapid, Convergent Approach to the Identification of Exosome Inhibitors in Breast Cancer Models.","authors":"Zoraida Andreu, Esther Masiá, David Charbonnier, María J Vicent","doi":"10.7150/ntno.73606","DOIUrl":"https://doi.org/10.7150/ntno.73606","url":null,"abstract":"Targeting cancer cell exosome release and biogenesis represents a potentially efficient means to treat tumors and prevent cancer recurrence/metastasis; however, the complexity and time-consuming nature of currently employed methods to purify and characterize exosomes represent obstacles to progression. Herein, we describe a rapid, convergent, and cost-efficient strategy to analyze candidate U.S. Food and Drug Administration (FDA)-approved drugs that inhibit exosome release and/or biogenesis using breast cancer cell line models in the hope of repurposing them for the clinical treatment of metastatic tumors. We combined the ExoScreen assay based on AlphaScreenTM technology with the antibody-mediated detection of an atypical lipid (lysobisphosphatidic acid - LBPA) present in the intra-luminal vesicle/exosomal fraction to achieve both extracellular and intracellular information on exosome modulation after treatment. As proof of concept for this strategy, we identified docetaxel, biscurcumin, primaquine, and doxorubicin as potential exosome release inhibitors in the Her-2 positive MDA-MB-453 and luminal A MCF7 cell lines. Dinaciclib also functioned as an exosome release inhibitor in MCF7 cells. Further, we explored the expression of proteins involved in exosome biogenesis (TSG101, CD9 tetraspanin, Alix, SMase2) and release (Rab11, Rab27) to decipher and validate the possible molecular mechanisms of action of the identified exosome inhibitors. We anticipate that our approach could help to create robust high-throughput screening methodologies to accelerate drug repurposing when using FDA-approved compound libraries and to develop rationally-designed single/combination therapies (including nanomedicines) that can target metastasis progression by modulating exosome biogenesis or release in various tumor types.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"7 1","pages":"1-21"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760366/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10691067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Pre-clinical Investigations of Therapeutic Markers Associated with Acute and Chronic Restraint Stress: A Nuclear Magnetic Resonance Based Contrast Metabolic Approach. 与急性和慢性约束应激相关的治疗标志物的临床前研究:基于核磁共振的对比代谢方法。

Nanotheranostics Pub Date : 2023-01-01 DOI: 10.7150/ntno.76294

Sanjay Singh, Sukanya Tripathy, Atul Rawat, Durgesh Dubey, Sarfraj Ahmad Siddiqui, Rajesh Ugale, Dinesh Kumar, Anand Prakash

{"title":"Pre-clinical Investigations of Therapeutic Markers Associated with Acute and Chronic Restraint Stress: A Nuclear Magnetic Resonance Based Contrast Metabolic Approach.","authors":"Sanjay Singh, Sukanya Tripathy, Atul Rawat, Durgesh Dubey, Sarfraj Ahmad Siddiqui, Rajesh Ugale, Dinesh Kumar, Anand Prakash","doi":"10.7150/ntno.76294","DOIUrl":"https://doi.org/10.7150/ntno.76294","url":null,"abstract":"Stress can be defined by two parameters, first the psychological sensing of pressure and second is the body's response. However, the exposure time to stress depicts the biological response produced against it. The effect of acute and chronic restraint stress on anxiety and the production of systemic metabolites were investigated in male Sprague-Dawley (SD) rats. Behavioural test was performed on elevated plus maze (EPM) in conjunction with the statistical analysis that exhibited the habituation during long term exposure to stress when compared with the short-term stress. These behaviour-based changes resulted in interpolated concentration of some serum metabolites like carbohydrates, amino acids and lipids as analysed by NMR. Metabolic analysis along with the multivariate analysis demonstrated that the expression of concentration of metabolites including glutamate, proline, succinate, citrate, and tyrosine is higher in the acute stress than the chronic stress, while glucose and lipids i.e., LDL and VLDL changed in the opposite trends. Thus, the aforesaid study provides an analytical strategy for the characterization of perturbed metabolites induced due to the behavioural modifications in an organism. It may further aid in developing potential therapeutic markers at the metabolic levels which may broaden the treatment options for stress and anxiety related disorders.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"7 1","pages":"91-101"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9760362/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10691068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Preclinical safety assessment of photoluminescent metal quantum clusters stabilized with autologous serum proteins for host specific theranostics. 自体血清蛋白稳定的光致发光金属量子团簇用于宿主特异性治疗的临床前安全性评估。

Nanotheranostics Pub Date : 2023-01-01 DOI: 10.7150/ntno.82978

Kritika Sood, Pranjali Yadav, Manu Jamwal, Reena Das, Asifkhan Shanavas

引用次数: 0

Peelable Microneedle Patches Deliver Fibroblast Growth Factors to Repair Skin Photoaging Damage. 可剥微针贴片提供成纤维细胞生长因子修复皮肤光老化损伤。

Nanotheranostics Pub Date : 2023-01-01 DOI: 10.7150/ntno.79187

Guojun Yang, Shiqi Hu, Haiyue Jiang, Ke Cheng

{"title":"Peelable Microneedle Patches Deliver Fibroblast Growth Factors to Repair Skin Photoaging Damage.","authors":"Guojun Yang, Shiqi Hu, Haiyue Jiang, Ke Cheng","doi":"10.7150/ntno.79187","DOIUrl":"https://doi.org/10.7150/ntno.79187","url":null,"abstract":"Rationale: UV light deeply penetrates the dermis, leading to inflammation and cell death with prolonged exposure. This is a major contributor to skin photoaging. In the pharmaceutical field, fibroblast growth factors (FGFs) have gained popularity for enhancing skin quality as they facilitate tissue remodeling and re-epithelization. Nonetheless, their effectiveness is significantly hindered by limited absorption. Methods: We have successfully created a dissolving microneedle (MN) patch that contains hyaluronic acid (HA) loaded with FGF-2 and FGF-21. This patch aims to improve the therapeutic efficiency of these growth factors while providing a simple administration method. We determined the performance of this patch in an animal model of skin photoaging. Results: The FGF-2/FGF-21-loaded MN (FGF-2/FGF-21 MN) patch demonstrated a consistent structure and suitable mechanical properties, allowing for easy insertion and penetration into mouse skin. Within 10 minutes of application, the patch released approximately 38.50 ± 13.38% of the loaded drug. Notably, the FGF-2/FGF-21 MNs exhibited significant improvements in UV-induced acute skin inflammation and reduced mouse skin wrinkles within a span of two weeks. Furthermore, the positive effects continued to enhance over a four-week treatment period. Conclusion: The proposed HA-based peelable MN patch provides an efficient approach for transdermal drug delivery, providing a promising method for improved therapeutic outcomes.","PeriodicalId":36934,"journal":{"name":"Nanotheranostics","volume":"7 4","pages":"380-392"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10327422/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9811198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0