Aquaculture and Fisheries最新文献

{"title":"Altered bacterial-fungal balance in the intestine of bullfrogs (Rana catesbeiana) with enteritis","authors":"Dongdong Wei ,&nbsp;Ke Ke ,&nbsp;Shuangyan Xiao ,&nbsp;Qing Yu ,&nbsp;Jingu Shi ,&nbsp;Shuyu Han ,&nbsp;Xiaohua Lu ,&nbsp;Zhongbao Guo ,&nbsp;Xu Xia ,&nbsp;Ming Chen ,&nbsp;Jianming Li ,&nbsp;Pengfei Li","doi":"10.1016/j.aaf.2025.01.006","DOIUrl":"10.1016/j.aaf.2025.01.006","url":null,"abstract":"<div><div>The present study aimed to elucidate the relationship between gut microbiota composition and enteritis in bullfrogs, which is a species of significant economic value in aquaculture. By comparing the gut microbiota of healthy bullfrogs (HG), bullfrogs with enteritis (DG), and bullfrogs in the wild (WG), we sought to identify the microbial community changes associated with enteritis and understand the dynamics of bacterial and fungal interactions in different environments. Utilizing high-throughput sequencing of 16S rRNA gene and ITS amplicons, we analyzed the gut microbiota of the three groups. Our findings showed that the Shannon index of bacterial communities in diseased bullfrogs was lower than that in healthy ones. Dominant bacterial phyla such as Proteobacteria, Bacteroidetes, and Firmicutes had varying abundances among the groups. Opportunistic pathogens like Aeromonas and Vibrio were enriched in DG, while beneficial bacteria were more abundant in HG. The bacterial network complexity is the highest in the WG group, the fungal network complexity is the highest in the HG group, and the complexity of bacteria-fungus network interaction is the highest in the DG group. Our research demonstrates significant alterations in the gut microbiota composition and interactions in bullfrogs with enteritis. The findings highlight the importance of maintaining a balanced microbial community in the gut to prevent enteritis. This study provides foundational insights into the microbial ecology of bullfrog intestines and offers potential strategies for managing and preventing enteritis in bullfrog aquaculture. This study reveals the microbial shifts in bullfrog intestines associated with enteritis, showing a disrupted bacterial and fungal balance that exacerbates disease. By identifying the enriched pathogens and the reduced beneficial bacteria in diseased bullfrogs, our research underscores the critical role of gut microbiota in health and disease. These insights are essential for developing targeted interventions to restore the microbial balance and prevent enteritis in bullfrog aquaculture, thereby enhancing economic sustainability.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 93-103"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutamate induces larval settlement of the pacific oyster Crassostrea gigas","authors":"Xueshu Zhang ,&nbsp;Yiran Si ,&nbsp;Linfang Zhang ,&nbsp;Deliang Li ,&nbsp;Chuanyan Yang ,&nbsp;Ning Kong ,&nbsp;Lingling Wang ,&nbsp;Linsheng Song","doi":"10.1016/j.aaf.2024.12.003","DOIUrl":"10.1016/j.aaf.2024.12.003","url":null,"abstract":"<div><div>The settlement of bivalve larvae is a complex process influenced by a combination of environmental, biological, and chemical factors, which is crucial for the development of the bivalve seedling industry. This study investigates the potential of glutamate (Glu) at various concentrations to induce settlement in Pacific oyster (<em>Crassostrea gigas</em>) mid-stage umbo larvae. The findings reveal that treating mid-stage umbo larvae with 10⁻⁴ M Glu for 6 h enhances growth and survival rates, significantly promotes the formation of eye spots, and accelerates settlement. Glu can effectively induce larval settlement, leading to the development of neural structures such as eye spots. This research confirms the efficacy of Glu as a larval settlement inducer in oysters, offering novel strategies for oyster seed production.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 40-44"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Profit or Loss? Delving into the cost-benefit dynamics of characteristic freshwater fish aquaculture in China","authors":"Baoting Peng,&nbsp;Xin Shen,&nbsp;Qijun Jiang","doi":"10.1016/j.aaf.2024.11.004","DOIUrl":"10.1016/j.aaf.2024.11.004","url":null,"abstract":"<div><div>With the rapid development of China's economy, the living quality and consumption levels of people have continuously improved. As a result, freshwater fish aquaculture has emerged as an important means of meeting the growing demand for high-quality aquatic products. In this context, this paper utilizes monitoring data from the sample demonstration areas of the national characteristic freshwater fish aquaculture technology system to conduct a comparative analysis of the cost and income situation of freshwater fish aquaculture from 2021 to 2022. The results are as follows: (1) In 2022, the total loss of the characteristic freshwater fish aquaculture increased by 68.04%, primarily attributed to disease losses and natural disasters; (2) In 2022, the overall cost of the characteristic freshwater fish aquaculture increased by 2.02%, with a 4.80% increase in unit costs, and the change of the aquaculture input cost mainly depended on the increase of feed costs; (3) In 2022, the overall cost margin, sales margin and marginal contribution rate of characteristic freshwater fish all decreased, and the revenue also decreased; (4) In 2022, the net profit of the characteristic freshwater fish, as well as each fish species, was highly sensitive to the price, feed cost and fry cost. Based on the research findings mentioned above, the characteristic freshwater fish industry faces problems such as high farming risks and persistently high feed costs, and this paper summarizes farming risks as disease risk, market risk and environmental risk. Therefore, to improve aquaculture efficiency and profitability, it is necessary to establish a sound fishery insurance system to enhance risk resilience, strengthen the development of the feed industry to reduce aquaculture costs and form a brand effect to play a leading role.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 209-219"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sinking performance of new biplane depressor for constant-depth ichthyoplankton sampler at horizonal sampling","authors":"Xin Zhuang ,&nbsp;Gaobo Guo ,&nbsp;Xinxing You ,&nbsp;Fuxiang Hu","doi":"10.1016/j.aaf.2024.11.005","DOIUrl":"10.1016/j.aaf.2024.11.005","url":null,"abstract":"<div><div>A constant-depth ichthyoplankton sampler is vital equipment for monitoring fish recruitment and assessing fishery stocks. Its depressor, a critical component, maintains towing stability during sampling by balancing the sinking force of the depressor and the drag force of the sampler. Conventional depressors often fail to maintain this equilibrium due to their limited sinking force. This study proposes a biplane depressor, inspired by the aerodynamics of biplane hyper-lift trawl doors. In the hydrodynamic experiment, the maximum sinking force coefficient for the proposed biplane depressor with a stagger angle of 30° reached 2.02, which was significantly higher than that for conventional depressors (less than 1.60). Moreover, substituting metal with canvas for 40% of the wing area reduced the weight without compromising sinking performance (maximum sinking force coefficient: 1.86). A towing experiment demonstrated a consistent sinking force and towing stability for the depressor with and without canvas. Operating angles of attack of 27° and 23° are recommended for the depressor made of all metal and a combination of metal and canvas, respectively. The results indicate the potential of the proposed biplane depressor to replace conventional depressors to enhance the constant-depth performance of the sampler and ensure reliable sample collection for ichthyoplankton surveys.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 170-179"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of the histology and Micro-CT scan of the goldfish hood","authors":"Jinghang Zhang ,&nbsp;Jingtong Zhou ,&nbsp;Yiling Pan ,&nbsp;Wei He ,&nbsp;Chenhong Li","doi":"10.1016/j.aaf.2024.10.002","DOIUrl":"10.1016/j.aaf.2024.10.002","url":null,"abstract":"<div><div>The hood (also known as head growth or wen) is a distinctive feature of goldfish characterized by skin tissue proliferation. The classification of goldfish hood types and the study of the underlying skeletal structure still have substantial gaps in research. This study aimed to investigate the structural differences in goldfish hood tissues using histological and Micro-CT comparisons, based on traditional Chinese classifications. Histological observations revealed two distinct types: the one-piece type (Crown Pearlscale), characterized by a uniformly thickened epidermis, and the fused type (Oranda, Red Cap Oranda, and Ranchu), which showed irregular epidermal extensions into the dermis. Micro-CT scans further demonstrated that hooded goldfish exhibited skull protrusions, bone spurs, and widening of the cranial region. These findings indicate that fibroblast activity, tissue edema, and bone structural changes may be key drivers of hood formation in these goldfish varieties.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 152-159"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GABAA and GABAB receptor agonists differentially regulate the reproductive axis in the black molly Poecilia sphenops","authors":"Achyutham Hotha,&nbsp;C.B. Ganesh","doi":"10.1016/j.aaf.2025.06.004","DOIUrl":"10.1016/j.aaf.2025.06.004","url":null,"abstract":"<div><div>Gamma-aminobutyric acid (GABA) regulates several physiological, neuroendocrine, and behavioural functions, yet the specific role of its receptors in the hypothalamic-pituitary-testis (HPT) axis remains unclear in teleosts. We investigated the effects of muscimol (MUSC, a GABA_A receptor agonist) and baclofen (BAC, a GABA_B receptor agonist) on the HPT axis in the male black molly <em>Poecilia sphenops.</em> Administration of 0.05 μg MUSC on alternate days for 21 days upregulated gonadotropin-releasing hormone-I (GnRH-I) mRNA levels in the preoptic-hypothalamus, whereas they were downregulated in 0.5 or 5 μg MUSC-treated groups. The GnRH-I mRNA levels were unaltered following treatment with 5 or 10 μg BAC, but they were upregulated in 50 μg BAC-treated fish. The luteinizing hormone-β (LH-β) mRNA levels were unaltered following MUSC or BAC treatment across all doses. The numbers of all germ cell types were significantly decreased in 5 μg MUSC-treated fish concomitant with significantly lower levels of testicular 11-ketotestosterone (11-KT). On the other hand, treatment with 50 μg BAC caused a significant increase in the majority of the germ cells and testicular levels of 11-KT compared to controls. Furthermore, <em>in vitro</em> 11-KT levels in the testis were significantly increased in MUSC + human chorionic gonadotropin (hCG)-treated fish compared to those of the MUSC-alone group; however, a similar treatment of BAC + hCG did not attenuate the inhibitory effect of BAC-alone treatment. These findings suggest that GABA_A and GABA_B receptor agonists produce differential effects on the HPT axis of the black molly. In particular, MUSC suppresses the GnRH-I mRNA expression and spermatogenesis process at higher doses, whereas BAC promotes these processes at high concentration.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 71-83"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized protocol for nuclei isolation from aquatic fish brain tissue for single-cell Genomic assays","authors":"He Jiao ,&nbsp;Jingting Qi ,&nbsp;Yuan Xu ,&nbsp;Chenyu Yan ,&nbsp;Zhichao Wu ,&nbsp;Liangbiao Chen ,&nbsp;Songqian Huang ,&nbsp;Peng Hu","doi":"10.1016/j.aaf.2024.12.001","DOIUrl":"10.1016/j.aaf.2024.12.001","url":null,"abstract":"<div><div>Single-cell sequencing technology has become increasingly prominent in aquaculture for investigating complex biological processes, such as development, reproduction, and disease resistance in aquatic species like Nile tilapia, an economically important fish. This technology reveals cellular heterogeneity across various biological functions. However, traditional enzymatic dissociation methods often fail to generate high-quality, intact single-cell suspensions from complex brain tissue, limiting single-cell research in this area. This study aims to optimize the isolation of single-cell nuclei from tilapia (<em>Oreochromis niloticus</em>) brain tissue. We systematically fine-tuned the parameters of homogenization buffer, washing buffer, and centrifugation conditions, identifying the optimal method for nuclei isolation from tilapia brain. We developed a streamlined, rapid, and efficient protocol that eliminates the need for costly equipment, such as flow cytometry and ultracentrifugation. As a proof of principle, we successfully identified the cellular heterogeneity of tilapia brain tissue using single-nucleus RNA-seq analysis. The method we developed yields intact nuclei with minimal background contamination and a broad representation of cell types, making it a reliable tool for single-cell genomic assays in aquatic fish species.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 33-39"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The linkage between innate and adaptive immunity: Recent advances in antigen recognition, processing, and presentation in fish","authors":"Liting Wu ,&nbsp;Jianmin Ye ,&nbsp;Jun Li","doi":"10.1016/j.aaf.2025.07.002","DOIUrl":"10.1016/j.aaf.2025.07.002","url":null,"abstract":"<div><div>Innate immunity in fish is critically important for preventing the entry of pathogenic microorganisms by adeptly recognizing pathogen-associated molecular patterns (PAMPs) through pattern recognition receptors (PRRs). Concurrently, the adaptive immune response equips the vertebrate immune system to identify and retain memory of specific pathogens, thereby facilitating enhanced secondary responses upon re-exposure. Antigen-presenting cells (APCs) are integral to this process, as they recognize antigens via mechanisms including PRRs, internalize them, and process these antigens for presentation to T cells. This interaction triggers the activation of both T cells and B cells, initiating a robust priming of the adaptive immune system and establishing a functional bridge between innate and adaptive immunity. Antigen presentation serves as a pivotal mechanism for T cell activation and B cell differentiation, thereby leading to the establishment of effective antimicrobial protection. Vaccination of fish is of paramount importance for preventing specific infectious diseases and is economically and environmentally essential for the development of a sustainable fish aquaculture industry. The design of efficacious vaccines necessitates the establishment of long-term protection against specific antigenic challenges, with APCs occupying a central role in this endeavor. This review summarizes the most recent studies on fish antigen presentation pathways and elucidates the mechanisms involved in the recognition, processing, and presentation of antigens by APCs, triggering activation of T cells. Moreover, this review highlights recent findings concerning immune regulatory factors that activate adaptive immunity, including adjuvants and immunostimulants, providing the prospects for fish vaccine applications. A comprehensive understanding of how fish APCs detect and respond to antigens will have profound implications for the future development of tailored vaccination strategies and the rational design of interventions against infectious diseases impacting the commercial aquaculture sector.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 1-10"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphology and structure of scales at the lateral-line region of Hyporhamphus limbatus in the Vietnamese Mekong Delta","authors":"Vinh Quoc Nguyen,&nbsp;Vuong Van Ly,&nbsp;Phuc Le Hoang Nguyen,&nbsp;Ly Thi Cam Tran,&nbsp;Quyet Le Nguyen,&nbsp;Quang Minh Dinh","doi":"10.1016/j.aaf.2025.06.001","DOIUrl":"10.1016/j.aaf.2025.06.001","url":null,"abstract":"<div><div>This study examines the morphology and structure of scales at the lateral-line region of <em>Hyporhamphus limbatus</em>, specifically the lateral-line scales (<em>lls</em>), dorsal-to lateral-line scales (<em>dol</em>), and ventral-to lateral-line scales (<em>vel</em>), based on 100 specimens collected from Phuoc Long - Bac Lieu and Dam Doi - Ca Mau between July and September 2024. After identifying the scales, their dimensions and fish total length were measured to investigate the regression relationship between these variables. The scales were subsequently dyed to observe and analyze their morphology and structure in greater detail. The results showed that <em>Hyporhamphus limbatus</em> had 40 to 69 lls (51 ± 5 SE), with a mean length of 0.51 ± 0.04 SE mm and width of 0.30 ± 0.01 SE mm. The species exhibited 36 to 52 <em>dol</em> (46 ± 6 SE scales), with an average radius of 2.09 ± 0.06 SE mm, and 41 to 57 <em>vel</em> (49 ± 2 SE scales), measuring 1.83 ± 0.02 SE mm. A regression analysis between the length of the lateral-line scales and the fish total length showed a positive relationship (<em>R</em>^<em>2</em> &gt; 0.90). This research on the morphology and structure of scales in <em>Hyporhamphus limbatus</em> aims to enhance understanding of sex-based growth patterns and differentiation. The findings also contribute to characterizing the fish scale diversity, providing a valuable resource for future studies on this species’ biological traits, mainly through examining its scales.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 84-92"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Decoding feed efficiency: Liver and intestinal transcriptomics in Larimichthys crocea fed a fishmeal-free diet","authors":"Ranxin Fu ,&nbsp;Gongsi Wang ,&nbsp;Xiaotong Li ,&nbsp;Xingcheng Zhu ,&nbsp;Peng Ren ,&nbsp;Lining Zhang ,&nbsp;Qinghui Ai ,&nbsp;Zhiyong Wang","doi":"10.1016/j.aaf.2025.05.006","DOIUrl":"10.1016/j.aaf.2025.05.006","url":null,"abstract":"<div><div>Feed efficiency (FE) is a key indicator of an animal's ability to convert feed into growth, directly impacting production costs and sustainability in aquaculture. This study aimed to explore the liver and intestinal transcriptomic profiles of <em>Larimichthys crocea</em> individuals with high FE (H group) and low FE (L group) under a fishmeal-free diet. Differentially expressed genes (DEGs) and associated regulatory pathways were identified to shed light on potential genetic factors influencing FE traits. Compared to the L group, 53, 24, and 112 DEGs were detected in the liver, proximal intestine, and distal intestine tissues of the H group, respectively. Notably, 24 up- and 29 down-regulated genes were found in the liver, 9 up- and 15 down-regulated genes in the proximal intestine, and 41 up- and 71 down-regulated genes in the distal intestine. GO and KEGG pathway analyses highlighted 15 key genes related to metabolism (<em>acsl3</em>、<em>fads2</em>、<em>acaca</em>、<em>gpam</em>、<em>cel、slc10a2</em>、<em>pemt</em>、<em>agpat3、agpat4</em>、<em>acot1</em> and <em>pla2g15</em>) and growth (<em>irs2</em>、<em>fgf10、rnf15</em>, and <em>deptor</em>) as potential candidates for improving FE in <em>L. crocea</em>. Quantitative PCR confirmed the up- or down-regulation of these genes in line with RNA-seq results. This study offers valuable insights into the molecular mechanisms underlying feed efficiency in <em>L. crocea</em> and identifies candidate genes for molecular breeding aimed at enhancing FE.</div></div>","PeriodicalId":36894,"journal":{"name":"Aquaculture and Fisheries","volume":"11 1","pages":"Pages 59-70"},"PeriodicalIF":0.0,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145651869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}