{"title":"Room temperature Impregnation with Cold Temperature Biodur® Silicone: A Study of Viscosity.","authors":"M. Șora","doi":"10.56507/qida3140","DOIUrl":"https://doi.org/10.56507/qida3140","url":null,"abstract":"Centre for Anatomy and Molecular Medicine, Sigmund Freud Private University, Vienna, Austria. ABSTRACT: There are two common methods for carrying out plastination with silicone polymer: coldtemperature impregnation (at -15 to -25°C) or impregnation at room temperature. The standard Biodur technique is the cold impregnation method, although some plastinators prefer to impregnate at room temperature. The aim of our study was to determine the viscosity of standard Biodur® silicone mixtures under different temperature conditions, in order to determine the optimum impregnation time. Two standard silicone mixtures were prepared: S10/S3, and S15/S3. Each silicone mixture was then divided into 3 equal parts in order to determine the viscosity at -25°C, room temperature (+20°C), and +40°C. Measurements of viscosity were carried weekly, for four weeks, using a rotary viscometer. The initial viscosity of S10/S3 at -25°C was 4 000 mPa.s, and it remained almost the same for the next 4 weeks. At room temperature, the viscosity reached 4 000 mPa.s after 4 weeks. At +40°C this value was reached after 10 days. The viscosity of the S15 mixture at -25°C increased to 455 mPa.s after one day and remained almost constant through the next 4 weeks. At room temperature the viscosity reached 400 mPa.s after 3 weeks, and at +40°C it increased to 400 mPa.s after 8 days Impregnation at room temperature is possible with both S10 and S15. For the S10 method, an impregnation period of 2 weeks would be recommended, With S15, impregnation at room temperature could be carried out over a period of 1 week, which would be possible for advanced plastinators. However, it is not recommended to plastinate nervous tissue at room temperature.","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44599248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"3-D Reconstruction of the Retrobulbar Orbital Septa Using Biodur E12®","authors":"","doi":"10.56507/cdac7411","DOIUrl":"https://doi.org/10.56507/cdac7411","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48122713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Biodur® S10/S3 and S15/S3 at “Room Temperature”: a viscosity study","authors":"","doi":"10.56507/rnbu9088","DOIUrl":"https://doi.org/10.56507/rnbu9088","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46504746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Farewell Letter from the President Carlos Baptista","authors":"","doi":"10.56507/qnhn1098","DOIUrl":"https://doi.org/10.56507/qnhn1098","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The 18th International Conference on Plastination Toledo, Ohio - USA June 27-July 1, 2016","authors":"","doi":"10.56507/dmdh6190","DOIUrl":"https://doi.org/10.56507/dmdh6190","url":null,"abstract":"","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70818875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hoffen P45: A Modified Polyester Plastination Technique for Both Brain and Body Slices","authors":"Hong-Jin Sui, R. Henry","doi":"10.56507/onmi1596","DOIUrl":"https://doi.org/10.56507/onmi1596","url":null,"abstract":"2 College of Veterinary Medicine, Lincoln Memorial University, 6965 Cumberland Gap Parkway, Harrogate, TN 37752,USA. ABSTRACT: Plastination is the premier methodology for preservation of biological specimens, and is applicable to many allied areas: anatomy, biology, pathology, embryology, and clinical medicine, as well as art. This polymer technique produces 2-3 mm semi-transparent to translucent slices which display anatomy within its normal relationships and anatomical environs. Polyester slices are an excellent modality for understanding modern diagnostic images: computed tomography, magnetic resonance and ultrasound. Polyester plastination was developed for the preservation and study of brain tissue. In recent years, polyester has also been used for presentation of numerous tissues. The Hoffen P45 technique was developed near the turn of the century for both brain tissue and body slices. Both the resin and the curing method are different from classic polyester techniques. The Hoffen P45 technique uses a water bath for curing of the polymer rather than UVA light.","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70818980","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Non-Perishable Museum Specimens: Redefined Plastination Technique","authors":"","doi":"10.56507/bret3411","DOIUrl":"https://doi.org/10.56507/bret3411","url":null,"abstract":"Department of Oral Pathology & Microbiology, Kothiwal Dental College and Research Center, Mora Mustaqueem, Kanth Road, Moradabad, U.P.-244001, India Objective: The study was conducted to overcome specific limitations of formalin-preserved specimens at negligible cost. Materials and Methods: The study was conducted on museum specimens collected from the Department of General Anatomy, Kothiwal Dental College. All the collected specimens were plastinated employing previously utilized laboratory consumables, such as disposable culture plates, and xylene, mixed together in a fixed proportion to form a homogeneous paste. After complete dehydration in alcohol, clearing was done in acetone followed by impregnation with reactive polymer under vacuum which was created with a modified suction apparatus. Curing of the specimen was done under direct sunlight. Results: This technique has proved its advantages over formalin-preserved specimens as it has produced dry, life-like specimens. The only limitation of our technique was that it has showed marked shrinkage after curing. Conclusion: We have devised a new and cost-effective method of plastination that involves no specialized equipment and prepares plastinated specimens that retain much of their natural features in a life-like manner.","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70818697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Coloring Muscles and Vessels of Plastinated Limbs with Colored Silicone to Supplement Teaching","authors":"J. Kang, S. Iliff, R. Henry, D. Hermey","doi":"10.56507/ltnc5138","DOIUrl":"https://doi.org/10.56507/ltnc5138","url":null,"abstract":"1 Department of Anatomy, Lincoln Memorial UniversityDeBusk College of Osteopathic Medicine, Harrogate, TN 37752 USA. 2 College of Veterinary Medicine, Lincoln Memorial University, Harrogate, TN USA ABSTRACT: Plastinated specimens, which have been painted or stained to highlight anatomical structures, can be helpful materials for medical students as well as others interested in studying anatomy. However, one problem with these specimens is that the paint often chips off due to being handled by many students and teachers. It may also wear off naturally if the specimen is kept for a long time. In this experiment, specimens were colored prior to the curing stage in an attempt to improve durability and better adherence of the color to the specimen. The results were then compared to techniques used by McCreary (2013). Colored specimens were challenged using methods which might be similar to routine handling during study. It was expected that the new coloring technique of applying the solution prior to the cross-linking stage instead of after the cross-linking stage would provide a stronger adhesion to the specimen. Although the color on the specimen applied prior to the cross-linking step demonstrated a more polished appearance compared to the color applied after the cross-linking stage, various durability tests confirmed no better efficacy or durability in adhesiveness of the silicone color to the specimen. Rather, some of the color came off when rubbed with fingers or latex-gloved hands. .","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70818902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Comparison of Cold and Room Temperature Silicone Plastination Techniques Using Tissue Core Samples and a Variety of Plastinates","authors":"D. Starchik, R. Henry","doi":"10.56507/ntqj7764","DOIUrl":"https://doi.org/10.56507/ntqj7764","url":null,"abstract":"2 College of Veterinary Medicine, Lincoln Memorial University, Harrogate, TN, USA ABSTRACT: A variety of organs, body regions and whole body specimens were plastinated using standard procedures for both cold and room temperature silicone plastination techniques. From these plastinates, advantages and shortcomings of both methods were evaluated. Criteria used for evaluation of plastinates included: duration of impregnation and curing, quality of plastinated specimens, need for extra equipment and its maintenance, as well as other cost considerations. To efficiently evaluate shrinkage and plastination duration, 3 cm pieces (core samples) of parenchymatous organs and 7 cm lengths from intestinal segments were collected, dehydrated and plastinated using standard procedures for both cold and room temperature silicone plastination techniques. Core sample volume was evaluated at the end of each stage of the process by fluid displacement. Shrinkage of samples was calculated after each stage of plastination. Evaluation of this information showed that the room temperature plastination technique takes about 35% less time for impregnation and curing, causes an average 8% less specimen shrinkage, produces life-like hair, fur or feathered specimens and it is more costefficient. The cold temperature plastination technique produces more flexible and elastic specimens and is preferable for whole body plastination.","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70818967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Mathematically Quantifying Learning Experience: Correlating Magnetic Resonance Imaging (MRI) and Plastinated Brain Sections Using Utility Analysis","authors":"Vijitashwa Pandey, V. Shukla, C. Baptista","doi":"10.56507/vbyi5508","DOIUrl":"https://doi.org/10.56507/vbyi5508","url":null,"abstract":"1 Department of Industrial and Systems Engineering Oakland University Rochester, MI 48309 USA 2,3 Department of Neurosciences College of Medicine, University of Toledo, Ohio, USA ABSTRACT: Objectives: Many researchers have shown that when used in conjunction, multiple pedagogic approaches increase student learning. Diagnostic imaging is used extensively to complement cadaveric dissection in courses such as neuroanatomy. This article provides a general framework to analyze and quantify the learning utility from combining multiple teaching methods for a richer learning experience. We present an example from neuroanatomy that combines the use of Magnetic Resonance Imaging and plastinated specimens. Materials and Methods: Two brains, from female cadavers aged between 70-90 years of age, were removed from the body, fixed in 10% formalin (mixture of 10 pbv of 37% formalin with 90 pbv water) and stored for at least 6 months before use. After six months, each brain was washed in tap-water overnight and sectioned coronally using a deli slicer. Slices measuring 10 mm in thickness were produced which were then plastinated using the standard S10/S3 silicone method. The plastinated brain slices were then used in conjunction with MRI images to analyze students’ preferences in neuroanatomy teaching. Results: Our method first aims to understand the tradeoff preferences of the educators and the students between multiple teaching methods. These preferences and tradeoff information can be incorporated into a learning utility function that brings a wealth of tools from decision analysis to analyze the proper allocation of teaching time between different methods. The synergistic effect of using multiple teaching tools in anatomy classes is, therefore, formally quantified. Conclusions: Using the example of MRI and plastinated specimens in neuroanatomy, we showed how one can analyze tradeoff between two modalities. In other words, one can determine how many hours of one modality can be traded off for another to have the same learning utility. One can also deduce the best allocation of a fixed total number of hours to maximize learning utility.","PeriodicalId":36740,"journal":{"name":"Journal of Plastination","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2015-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70819333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}