Frontiers in Sustainable Food Systems最新文献

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Considering the food environment can help to promote the consumption of aquatic foods for healthy diets 考虑食物环境有助于促进食用水产食品,促进健康饮食
IF 4.7 2区 农林科学
Frontiers in Sustainable Food Systems Pub Date : 2023-12-29 DOI: 10.3389/fsufs.2023.1241548
Gina Kennedy, Molly B. Ahern, Lora L. Iannotti, Sydney Vie, Lisa Sherburne, S. Thilsted
{"title":"Considering the food environment can help to promote the consumption of aquatic foods for healthy diets","authors":"Gina Kennedy, Molly B. Ahern, Lora L. Iannotti, Sydney Vie, Lisa Sherburne, S. Thilsted","doi":"10.3389/fsufs.2023.1241548","DOIUrl":"https://doi.org/10.3389/fsufs.2023.1241548","url":null,"abstract":"Aquatic foods ensure food and nutrition security for billions of consumers around the world. As part of food systems, aquatic foods provide nutritious, affordable, convenient options for healthy diets, and can also foster sustainable food production. Within the food system framework, the food environment is the space that connects food procurement to consumption. The food environment influences consumer decisions on which foods to acquire. To date there has been relatively little focus on creating an enabling food environment that supports consumers in decisions to obtain aquatic foods. To fill this gap, we conducted a narrative review of literature from 2000–2020 to document the availability, affordability, convenience, promotion, quality and sustainability of aquatic foods within diverse food environments. Our review highlighted several opportunities that can support development and promotion of convenient, high quality aquatic foods. We also noted several research gaps. For example, some consumers, especially those in high income countries, respond well to labels related to sustainability and also to messaging to consume diverse types of fish, especially lower tropic species like anchovy. However, less is documented on how promotion influences consumers from LMIC. The paper also notes a gap in assessment of the price and affordability of aquatic foods. Most price and affordability assessments do not provide details on which aquatic foods were considered in the costing assessment. In addition, wild or home-harvested aquatic foods are often not accounted for in price and affordability assessments. Using case studies, we demonstrate how considering the food environment in research and implementation strategies can add value to program design. For example, processing tuna frames and underutilized small fish species into powder is one innovation that reduces food waste and also creates a convenient, quality product. These results provide the foundation for deepening our understanding of how key elements of the food environment influence consumers’ decision-making and how these elements can be considered in future research, programming and policy efforts.","PeriodicalId":36666,"journal":{"name":"Frontiers in Sustainable Food Systems","volume":"223 4","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139145303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Effect of period of receptivity and air temperature on parthenocarpic phenomenon of ‘Assiane’ date palm cultivar (Phoenix dactylifera L.) 受精期和气温对'Assiane'枣椰栽培品种(Phoenix dactylifera L.)孤雌生殖现象的影响
IF 4.7 2区 农林科学
Frontiers in Sustainable Food Systems Pub Date : 2023-12-22 DOI: 10.3389/fsufs.2023.1326141
Imane Zahiri, F. Alla, M. N. AlZain, Abdulsalam Alhalmi, Omar Noman, Mohammed Khalid Parvez, M. A. Elhoumaizi
{"title":"Effect of period of receptivity and air temperature on parthenocarpic phenomenon of ‘Assiane’ date palm cultivar (Phoenix dactylifera L.)","authors":"Imane Zahiri, F. Alla, M. N. AlZain, Abdulsalam Alhalmi, Omar Noman, Mohammed Khalid Parvez, M. A. Elhoumaizi","doi":"10.3389/fsufs.2023.1326141","DOIUrl":"https://doi.org/10.3389/fsufs.2023.1326141","url":null,"abstract":"In hot arid regions, the productivity of certain date palm cultivars faces a significant challenge wherein non-fertilized flowers can give rise to parthenocarpic singular or triplet fruit.The aims of this study, we aimed to investigate the impact of delayed pollination on date palm yield and fruit quality, while also examining the influence of temperature on these processes.We conducted this research using 10 15-year-old ‘Assiane’ palm trees, all situated within the Figuig Oasis environment. Pollination was performed on the same day of spathe opening, as well as up to 16 days after the initial opening. Additionally, we utilized 30 palm trees to analyze the effect of daily average temperatures.Our findings reveal that the optimal period for achieving maximum yield and fruit quality falls between the sixth and eighth day following the cracking of the female spathe. Furthermore, we observed that relatively lower temperatures, ranging from 5 to 20°C, promote the formation of parthenocarpic fruits while inhibiting the development of normal fruits. Moreover, the insights gained from investigating these issues could have broader implications for date palm cultivation in arid regions and offer valuable lessons for the preservation of oasis ecosystems worldwide.","PeriodicalId":36666,"journal":{"name":"Frontiers in Sustainable Food Systems","volume":"8 38","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138994172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatio-temporal variability of climatic variables and its impacts on rice yield in Bangladesh 孟加拉国气候变量的时空变异性及其对水稻产量的影响
IF 4.7 2区 农林科学
Frontiers in Sustainable Food Systems Pub Date : 2023-12-22 DOI: 10.3389/fsufs.2023.1290055
Md Abdullah Al Mamun, S. Nihad, Md Abdur Rouf Sarkar, M. Sarker, Jitka Skalicka, Milan Skalicky
{"title":"Spatio-temporal variability of climatic variables and its impacts on rice yield in Bangladesh","authors":"Md Abdullah Al Mamun, S. Nihad, Md Abdur Rouf Sarkar, M. Sarker, Jitka Skalicka, Milan Skalicky","doi":"10.3389/fsufs.2023.1290055","DOIUrl":"https://doi.org/10.3389/fsufs.2023.1290055","url":null,"abstract":"Bangladesh is a significant contributor to the global food basket but is also one of the most vulnerable countries to climate change. Understanding regional climatic variability helps reduce climate risks and ensure food security. This research examined monthly, seasonal, and yearly temperature, rainfall, and relative humidity in Bangladesh using data from 35 meteorological stations from 1970–2020. The study utilized the Mann–Kendall method to evaluate trends and employed Sen’s slope to quantify their magnitude. Additionally, quantile regression was applied to analyze the impact of climatic variables on rice yield. The findings revealed that maximum (Tmax) and minimum (Tmin) temperatures were steadily increasing, with the southwest experiencing a more rapid rise compared to other regions in Bangladesh. Seasonal Tmax and Tmin rose in most parts of Bangladesh, particularly during the monsoon. In most areas, there was a significant (p < 0.05) increase in Tmax from June to September and Tmin in December. The variability of minimum temperature was considerably affected by warming throughout the country. The eastern area had the most significant (p < 0.05) annual rainfall increase rate, while the northern region had the lowest. There was evidence of inter-seasonal rainfall shipment, with post-monsoon rainfall rising compared to monsoon season. The quantile regression showed that the rice yield of Aus (summer) and Aman (wet) seasons were influenced by maximum temperature and relative humidity, whereas the rice yield of the Boro (dry) season was affected by maximum temperature, rainfall, and relative humidity. Across geographical segmentation, the regression analysis indicated a high level of variability in the northern climate. Overall, the intensity of mean temperature increased throughout the country. So, there is a need to develop heat or drought-resistant rice varieties and modernized irrigation facilities to mitigate these climate risks. However, farmers should employ automated weather-based advisory services for sustaining rice productivity and food security.","PeriodicalId":36666,"journal":{"name":"Frontiers in Sustainable Food Systems","volume":"33 38","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138946704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Understanding the motives behind the consumption of organic products in North Portugal: a focus group study 了解葡萄牙北部消费有机产品的动机:焦点小组研究
IF 4.7 2区 农林科学
Frontiers in Sustainable Food Systems Pub Date : 2023-12-22 DOI: 10.3389/fsufs.2023.1316634
Lucía Penalba-Sánchez, Elisa Di-Gregorio, Raquel Claro, Mafalda Pinto, Elisabete Pinto, Patrícia Oliveira-Silva
{"title":"Understanding the motives behind the consumption of organic products in North Portugal: a focus group study","authors":"Lucía Penalba-Sánchez, Elisa Di-Gregorio, Raquel Claro, Mafalda Pinto, Elisabete Pinto, Patrícia Oliveira-Silva","doi":"10.3389/fsufs.2023.1316634","DOIUrl":"https://doi.org/10.3389/fsufs.2023.1316634","url":null,"abstract":"The soil is a non-renewable and essential resource for life on our planet. Considering the widespread fast pace of soil degradation and erosion, it is urgent to protect it by enacting pro-environmental behaviors. Consumers’ massive purchase and consumption of organic products is a powerful way to encourage farmers to apply sustainable soil practices. Unfortunately, there is a lack of studies in Portugal explaining the increased interest but low consumption of organic products.Here, the relationship between (a) intrinsic motivations, extrinsic motivations, knowledge, and self-perception of control regarding pro-environmental behaviors, and (b) consumption of organic products and other related pro-environmental behaviors was explored. To do so, two semi-structured interview focus groups (n = 15 participants) were conducted.Organic product purchase and consumption seem to be driven by intrinsic motivations such as health or environmental concerns. External aspects such as labels and price as well, as personal, and psychological elements like knowledge and self-control, may be attenuating or strengthening the behavior. These findings should be validated in quantitative studies.","PeriodicalId":36666,"journal":{"name":"Frontiers in Sustainable Food Systems","volume":"20 6","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138947196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Syntrophy between bacteria and archaea enhances methane production in an EGSB bioreactor fed by cheese whey wastewater 细菌和古细菌之间的协同作用提高了以奶酪乳清废水为原料的 EGSB 生物反应器中的甲烷产量
IF 4.7 2区 农林科学
Frontiers in Sustainable Food Systems Pub Date : 2023-12-22 DOI: 10.3389/fsufs.2023.1244691
María Emperatriz Domínguez-Espinosa, Abumalé Cruz-Salomón, J. A. Ramírez De León, Jesús Mauricio Ernesto Hernández-Méndez, M. G. Santiago-Martínez
{"title":"Syntrophy between bacteria and archaea enhances methane production in an EGSB bioreactor fed by cheese whey wastewater","authors":"María Emperatriz Domínguez-Espinosa, Abumalé Cruz-Salomón, J. A. Ramírez De León, Jesús Mauricio Ernesto Hernández-Méndez, M. G. Santiago-Martínez","doi":"10.3389/fsufs.2023.1244691","DOIUrl":"https://doi.org/10.3389/fsufs.2023.1244691","url":null,"abstract":"The cheese-making process generates large amounts of cheese whey wastewater (CWW), which is abundant in nutrients but difficult to dispose of, contributing to the eutrophication of natural environments due to inadequate waste management. Here we show the anaerobic digestion of CCW by syntrophy between bacteria and archaea in an expanded granular sludge bed (EGSB) bioreactor as a low-cost alternative for bioremediation and biofuel production. The performance of the EGSB bioreactor and the composition of the natural microbial community were evaluated. During the operation of the EGSB bioreactor, physicochemical parameters such as alkalinity ratio (0.25), pH (7.5), and temperature (26°C) were attained and maintained, as well as light- and oxygen-free conditions, which favored the metabolism of oxygen-sensitive bacteria and methane-producing archaea (methanogens). Under these conditions, the chemical oxygen demand (COD) removal rate was highly efficient (> 89%). Methane (CH4) was produced from organic matter degradation by a few methanogens, mainly from Methanosaeta spp., and was enhanced by the metabolic interaction between bacteria and archaea. The biochemical methane potential (BMP) was >335 mL CH4/gCOD, indicating that the syntrophic microbial community is very efficient in removing organic matter and CH4 produced from CWW. Our results suggest that CWW could be treated in EGSB bioreactors and used as a sustainable alternative to CH4 production and also provide insights for the design of synthetic microbial communities (SynComs) for bioremediation, biogas production, and other biotechnological processes.","PeriodicalId":36666,"journal":{"name":"Frontiers in Sustainable Food Systems","volume":"53 31","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138946175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The future of African wild fruits – a drive towards responsible production and consumption of the marula fruit 非洲野生水果的未来--推动负责任地生产和消费马鲁拉果
IF 4.7 2区 农林科学
Frontiers in Sustainable Food Systems Pub Date : 2023-12-22 DOI: 10.3389/fsufs.2023.1294437
Edwin Hlangwani, P. H. Hal, Kgabo L. M. Moganedi, B. Dlamini
{"title":"The future of African wild fruits – a drive towards responsible production and consumption of the marula fruit","authors":"Edwin Hlangwani, P. H. Hal, Kgabo L. M. Moganedi, B. Dlamini","doi":"10.3389/fsufs.2023.1294437","DOIUrl":"https://doi.org/10.3389/fsufs.2023.1294437","url":null,"abstract":"The marula fruit has played a central role in the dietary intake, socioeconomic standing, and cultural practices of communities in southern Africa for over 10,000 years. The marula fruit is rich in nutritional compounds such as vitamins, minerals, organic acids, and amino acids which contribute to the health properties of the final products. These products can be sold informally by locals or developed into commercially viable, export products. The recent domestic and international demand for marula fruit-based products has raised sustainability concerns such as over-harvesting, habitat destruction, and unsustainable production practices. Given these challenges, a responsible and inclusive approach to marula production and consumption is required to meet the nutritional needs and sustainably contribute to the socioeconomic development of these communities. As a result, a scoping review approach was used to map out the literature on the production and consumption of the marula fruit. All reviewed literature explicitly focused on the marula fruit, the responsible use of the marula fruit, and sustainability practices in the production and consumption of marula fruit-based products. Furthermore, this review examined the potential benefits and challenges of expanding the production and consumption of marula products and identified strategies for promoting sustainable practices and equitable distribution of benefits. Despite the challenges in pre-and-postharvest processing, the marula fruit has remained a valuable resource. Thus, the sustainable production and consumption of the marula fruit require a holistic approach that addresses these challenges and promotes sustainable practices and equitable distribution of benefits. Central to this approach is the application of various technologies to establish robust value chains so the marula fruit industry can thrive.","PeriodicalId":36666,"journal":{"name":"Frontiers in Sustainable Food Systems","volume":"38 7","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138946844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mechanism of enhanced freshness formulation in optimizing antioxidant retention of gold kiwifruit (Actinidia chinensis) harvested at two maturity stages 在两个成熟阶段采收的黄金猕猴桃(Actinidia chinensis)中采用增强保鲜配方优化抗氧化剂保留的机制
IF 4.7 2区 农林科学
Frontiers in Sustainable Food Systems Pub Date : 2023-12-21 DOI: 10.3389/fsufs.2023.1286677
Sisanda S. L. Mthembu, L. S. Magwaza, S. Tesfay, A. Mditshwa
{"title":"Mechanism of enhanced freshness formulation in optimizing antioxidant retention of gold kiwifruit (Actinidia chinensis) harvested at two maturity stages","authors":"Sisanda S. L. Mthembu, L. S. Magwaza, S. Tesfay, A. Mditshwa","doi":"10.3389/fsufs.2023.1286677","DOIUrl":"https://doi.org/10.3389/fsufs.2023.1286677","url":null,"abstract":"Kiwifruit exhibits a climacteric ripening pattern and has as an extremely perishable nature. Considering that high perishability leads to a loss in antioxidants and overall nutritional quality. This study aimed to examine the efficacy of enhanced freshness formulation (EFF), a hexanal-based formulation containing antioxidants such as geraniol, α-tocopherol and ascorbic acid, on maintaining the bioactive compounds of gold kiwifruit (cv. ‘Y368’) harvested at two maturity stages. Kiwifruits were treated with three treatments, namely, control (untreated fruit), 0.01 and 0.02% (v/v) EFF. Fruits were treated with 8 weeks of cold storage at 0°C and 90% relative humidity, then transferred to 20°C for 8 days. Three bioactive compounds (ascorbic acid, total phenolics and flavonoids), antioxidant capacities using DPPH and FRAP assays, polyphenol oxidase, ascorbate oxidase, phenylalanine and tyrosine ammonia lyase enzyme activities were evaluated. The results showed that EFF significantly (p < 0.05) influenced bioactive compounds, antioxidant capacities and the activity of enzymes involved in the synthesis and oxidation of bioactive compounds. The maturity stage significantly influenced the content of bioactive compounds. Later harvested kiwifruit had greater content of bioactive compounds, compared to earlier harvested kiwifruit. The total phenolic content was 0.77, 1.09 and 1.22 mg GAE g−1 FW for control, 0.01 and 0.02% EFF, respectively. The FRAP antioxidant concentration was 0.76, 0.91 and 0.96 μmol Fe (II) g−1 FW for control, 0.01 and 0.02% EFF. The findings illustrate the capacity of EFF to optimize bioactive compounds and storability of kiwifruit during postharvest storage.","PeriodicalId":36666,"journal":{"name":"Frontiers in Sustainable Food Systems","volume":"57 23","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138950966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genome editing to re-domesticate and accelerate use of barley crop wild relatives 通过基因组编辑实现大麦作物野生近缘种的再驯化和加速利用
IF 4.7 2区 农林科学
Frontiers in Sustainable Food Systems Pub Date : 2023-12-21 DOI: 10.3389/fsufs.2023.1331577
Tobias Hanak, Jovana Janjić, Fiona R. Hay, Henrik Brinch-Pedersen
{"title":"Genome editing to re-domesticate and accelerate use of barley crop wild relatives","authors":"Tobias Hanak, Jovana Janjić, Fiona R. Hay, Henrik Brinch-Pedersen","doi":"10.3389/fsufs.2023.1331577","DOIUrl":"https://doi.org/10.3389/fsufs.2023.1331577","url":null,"abstract":"Climate change threatens global food security, but the biggest impact will be in arid, low social-economic regions. To improve food security, new breeding technologies (NBTs) could be implemented for re-domestication of crop wild relatives (CWR). CWR harbor many beneficial traits, but it is difficult to incorporate these traits into conventional breeding programs. Thus, although genebanks hold significant collections of CWR, their potential has yet to be reached. Using barley as an example, we describe how using genebank collections, digital sequence information and NBTs, re-domesticated barley can be produced with improved characteristics, while retaining the resilience and adaptation of the original material. Lastly, we highlight some obstacles that need to be overcome for re-domesticates to be adopted.","PeriodicalId":36666,"journal":{"name":"Frontiers in Sustainable Food Systems","volume":"64 1","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138952214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impacts of palm oil trade on ecosystem services: Cameroon as a case study 棕榈油贸易对生态系统服务的影响:喀麦隆案例研究
IF 4.7 2区 农林科学
Frontiers in Sustainable Food Systems Pub Date : 2023-12-21 DOI: 10.3389/fsufs.2023.1289431
A. N. Acobta, L. Ayompe, B. N. Egoh
{"title":"Impacts of palm oil trade on ecosystem services: Cameroon as a case study","authors":"A. N. Acobta, L. Ayompe, B. N. Egoh","doi":"10.3389/fsufs.2023.1289431","DOIUrl":"https://doi.org/10.3389/fsufs.2023.1289431","url":null,"abstract":"Palm oil (PO) producing countries are expanding oil palm cultivated areas to meet growing demands at the expense of tropical forests and the ecosystem services (ES) they provide. Current responses to the growing call for sustainable PO trade are based on environmental impacts such as deforestation, partly because most social impacts have not been studied. These responses are based on information from Asia and South America since little has been done in Africa. This study fills these gaps by synthesizing the impacts of PO trade on ES from peer-reviewed and gray literature. Our case study is Cameroon, which harbors part of the Congo basin forest and experiences fast deforestation rates. Fifty-three sources of literature were used for this study (53% peer-reviewed and 47% gray literature). We found that oil palm cultivation was reported to negatively affect 15 ecosystem services in 147 instances (85%) and positively affect seven ecosystem services in 25 instances (15%). The majority of negative impacts were on carbon sequestration and climate regulation (20%), habitat quality (13%) and genetic diversity (13%). The most positive impact was on food provision (8%). These results highlight the trade-offs between food provision and other ES. While current policy responses have focused on environmental impacts, many negative social impacts are associated with PO trade that should be addressed within new policy tools.","PeriodicalId":36666,"journal":{"name":"Frontiers in Sustainable Food Systems","volume":"36 24","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138952645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A simple and sensitive fluoroimmunoassay based on the nanobody-alkaline phosphatase fusion protein for the rapid detection of fenitrothion 基于纳米体-碱性磷酸酶融合蛋白的简单灵敏的荧光免疫测定,用于快速检测杀螟松
IF 4.7 2区 农林科学
Frontiers in Sustainable Food Systems Pub Date : 2023-12-21 DOI: 10.3389/fsufs.2023.1320931
Zijian Chen, Ai-Jun Huang, Xiu-Xiu Dong, Yi-Feng Zhang, Lin Zhu, Lin Luo, Zhen-Lin Xu, Hongwu Wang
{"title":"A simple and sensitive fluoroimmunoassay based on the nanobody-alkaline phosphatase fusion protein for the rapid detection of fenitrothion","authors":"Zijian Chen, Ai-Jun Huang, Xiu-Xiu Dong, Yi-Feng Zhang, Lin Zhu, Lin Luo, Zhen-Lin Xu, Hongwu Wang","doi":"10.3389/fsufs.2023.1320931","DOIUrl":"https://doi.org/10.3389/fsufs.2023.1320931","url":null,"abstract":"Immunoassay is a powerful tool for the rapid detection of small harmful organic molecules. In this study, a simple and sensitive fluoroimmunoassay (FIA) based on a nanobody-alkaline phosphatase fusion protein (VHHjd8-ALP) and blue-emissive carbon dots (bCDs) was developed for the rapid detection of fenitrothion. The bCDs were synthesized using the one-step hydrothermal method. Citric acid and urea were used as carbon and nitrogen sources, respectively. The synthesized bCDs were characterized by fluorescence spectrum, high-resolution transmission electron microscopy, x-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy. After one step of competitive immunoassay, the VHHjd8-ALP bound to the microplate and catalyzed the substrate p-nitrophenylphosphate (pNPP) into p-nitrophenol (pNP); the latter can quench the blue of bCDs due to an inner-filter effect. After condition optimization, an FIA calibration curve was finally created, which showed an IC50 value of 16.25 ng/mL and a limit of detection (LOD) of 0.19 ng/mL. Compared with the pNPP-based one-step conventional indirect competitive enzyme-linked immunoassay (icELISA), the developed FIA showed an 11-fold sensitivity improvement. Furthermore, the analysis period of FIA only takes approximately 55 min, which was obviously faster than that of the conventional icELISA. The recovery test showed recoveries from 81.8 to 119% with fruits and vegetable samples, which verified the practicability and accuracy of the developed FIA.","PeriodicalId":36666,"journal":{"name":"Frontiers in Sustainable Food Systems","volume":"135 45","pages":""},"PeriodicalIF":4.7,"publicationDate":"2023-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138953370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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