Indonesian Aquaculture Journal最新文献

{"title":"Nitrogen and Carbon Removal Capacity by Farmed Kelp Alaria marginata and Saccharina latissima Varies by Species","authors":"Schery Umanzor, Tiffany A. Stephens","doi":"10.3390/aquacj3010001","DOIUrl":"https://doi.org/10.3390/aquacj3010001","url":null,"abstract":"An increasing body of evidence shows that seaweeds, including kelp, can be used as a tool to neutralize or remove excess nutrients and metals from the water column. Here we report on a preliminary field assessment showing potential nutrient and carbon removal differences in sugar kelp and ribbon kelp grown in common gardens. Seawater and tissue samples were collected systematically from two farms in Alaska. Results show differences between the %N and %C content between Alaria marginata and Saccharina latissima. Results also show that tissue nitrogen in ribbon kelp varies sharply due to nitrogen availability in the water column. In contrast, the percentage of tissue N in sugar kelp remains comparatively stable. Our outcomes provide insight into potential differences in nutrient removal and harvest timing for different kelp species.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73980078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of an Enzyme Complex-Treated Rice Protein Concentrate on Growth Performance and Feed Utilization of Rainbow Trout Oncorhynchus mykiss Juveniles","authors":"Gunes Yaman Yoldas, S. Ueda, T. Takino, Tae-Su Seong, N. Kabeya, S. Satoh, Y. Haga","doi":"10.3390/aquacj2040019","DOIUrl":"https://doi.org/10.3390/aquacj2040019","url":null,"abstract":"This study investigated the effect of the inclusion of an enzyme complex-treated rice protein concentrate (RPC) in an extruded diet of juvenile rainbow trout (Oncorhynchus mykiss). A mixture of RPC, corn gluten and soybean meal was pretreated with an enzyme complex before extrusion processing of the diets. An enzyme complex-pretreated RPC (5, 10 and 15%) was formulated with 20% fish meal. A diet without RPC was used as a control. A total of 240 rainbow trouts with an average body weight of 6.04 g were placed in 12 rectangular glass aquaria and fed one of the experimental diets at apparent satiation for 12 weeks. There were no significant differences in growth, feed intake and survival among the groups. Slightly inferior weight gain and specific growth rate were observed in the RPC15E group compared to those in the other groups. The protein and ash contents of the whole body of the final fish were not significantly different among all groups, but the lipid content was significantly lower than that of the control when the dietary RPC level was >10%. Protein digestibility was negatively affected by increased RPC levels in diet. Significantly lower phosphorus digestibility was observed in fish fed the diet containing 5% enzyme-treated RPC than the control. Although there was no significant difference in lipid digestibility in all groups, significantly lower lipid retention was observed in fish fed a diet formulated with more than 10% RPC. These results suggest that the inclusion of RPC in the diet affects lipid retention and the content of rainbow trout. It also decreased protein digestibility. In conclusion, rainbow trout can be fed an extruded diet formulated with 20% fishmeal and 10% enzyme-treated RPC without negative effects on fish growth.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88911924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrical Stunning Has the Potential to Delay Fillet Degradation Post-Harvest in Red Seabream (Pagrus major)","authors":"Rafael Angelakopoulos, A. Dimitroglou, L. Papaharisis, K. Moutou","doi":"10.3390/aquacj2040018","DOIUrl":"https://doi.org/10.3390/aquacj2040018","url":null,"abstract":"Ensuring the humane harvest of farmed fish without compromising the quality of the fresh product is paramount to the welfare of fish and in meeting consumer demands. Electrical stunning is a quick and effective way to render fish unconscious and it has emerged as the suggested harvest method by EFSA and OIE. The present study evaluated the effects of electrical stunning on the biochemical processes that lead to fillet degradation postmortem, in the red seabream (Pagrus major). Two distinct electrical stunning conditions (low and high) were compared along with the conventional harvest method (an ice slurry). The activity patterns of calpain, collagenase, and cathepsin B and L were assessed and compared to stereological changes in white muscles at different time points up to 13 days post-harvest. Histological examinations, independent of the harvest technique, revealed a progressively declining trend in fiber volume density and increasing interfibrillar spaces over time, indicative of degradation activity within and between the muscle fibers. Strong correlations between the stereological measures and the individual protease activities were recorded. The higher current condition (electric field 1.8 V/cm and velocity 1.6 m/s) consistently exhibited the lowest protease activity levels and the slowest pace of stereological changes, making it the suggested method of all harvest methods explored.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72513313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of pH, N, P, N: P Ratio, and Dissolved Inorganic Carbon on Ulva ohnoi Growth and Biomass Quality: Potential Implications in IMTA-RAS","authors":"A. Alamrousi, E. Casais, Érika García-Cardesín, Ingrid Masaló, J. Pintado, J. Cremades","doi":"10.3390/aquacj2040017","DOIUrl":"https://doi.org/10.3390/aquacj2040017","url":null,"abstract":"Ulva ohnoi has a big potential in IMTA-RAS fish–seaweed systems. In order to design the best production strategy in these systems, the effect of the main environmental factors, such as pH, nutrient concentration (N, P, and N: P ratios) and dissolved inorganic carbon (DIC), on the productivity, bio filtration capacity, and quality of the biomass obtained was studied. It is concluded that in closed systems, strong pH variations (7.9–10.1) do not influence the growth of U. ohnoi and growth is slowed down due to the depletion of DIC. This fact would not be a problem in IMTA-RAS fish–macroalgae systems, due to the physiological activity of the fish contributing CO2 to the medium and replenishing it. The results obtained in the wide range of N: P ratios tested (2–410), allow us to conclude that this ratio should not be a limiting factor for the cultivation of Ulva ohnoi in IMTA-RAS systems. Based on those results, the best strategy to follow in an IMTA-RAS sole–sea lettuce would be to maintain the algae with highest level of nitrogen. This procedure implies a high rate of water renewal, which would also guarantee the maintenance of an adequate DIC and the best commercial quality of seaweed.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83981375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, China","authors":"Qiushui Wang, Xin‐wei Zhou, Yue Liu, Qi Ding, Zan Wu, Jie Deng, Jia Zuo, Liyan Yuan, Peng Shao, Bo Cheng, Lijuan Gao","doi":"10.3390/aquacj2040016","DOIUrl":"https://doi.org/10.3390/aquacj2040016","url":null,"abstract":"The continuous expansion of freshwater fish culture is confronted with environmental problems owing to their high antibiotic inputs and antibiotic-resistant bacteria. This study explored the antibiotic resistance gene (ARG) profiles of water and fish gut contents from three fishponds in Tianjin using a metagenomics approach. Proteobacteria, Bacteroidetes, and Actinobacteria were the most abundant phyla in all water samples. However, the microbial composition of the fish guts was distinctly different among the three aquaculture farms. Cetobacterium, Bacillus, Weissella, and Fusobacterium were the dominant genera in the gut contents of all fish. More than 20 unique ARGs with relatively high abundances were detected in both water and fish gut content samples. The dominant genes and pathways of antibiotic resistance mechanisms detected in all samples were antibiotic efflux, antibiotic inactivation, antibiotic target alteration, antibiotic target protection, antibiotic target replacement, and reduced permeability to antibiotics. In addition, our results indicate that antibiotics, such as florfenicol, and heavy metals, such as Zn and Cu, could have a significant correlation with some common ARGs, indicating that antibiotic-resistant bacteria could co-occur with heavy metals. Our study provides a research basis for the development of a strategy for practical antibiotic application and heavy metal monitoring in aquaculture.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90764739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effects of Origanum vulgare L. Essential Oils on Anaesthesia and Haemato-Biochemical Parameters in Mozambique Tilapia (Oreochromis mossambicus) Post-Juveniles","authors":"N. N. Gabriel, Gadaffi M. Liswaniso, Wilhelm Haihambo, K. Abasubong","doi":"10.3390/aquacj2040015","DOIUrl":"https://doi.org/10.3390/aquacj2040015","url":null,"abstract":"This study investigated the effects of oregano (Origanum vulgare) essential oil (OEO) anaesthesia and stress-related physiological parameters in tilapia mossambicus (Oreochromis mossambicus) post-juveniles. Fish were subjected to different concentrations of OEO (25, 50, 100, and 150 μL L−1) to assess the effects of the anaesthesia and recovery time. A second experiment subjected fish to other handling treatments, including a control (no OEO exposure), ethanol solution, 25 μL L−1 (low effective anaesthesia concentration of OEO), and 100 μL L−1 high effective anaesthesia concentration of OEO) for 10 min to assess the haemato-biochemical indices and survival rate at 0 h and after 24 h. Moreover, all the tested OEO concentrations induced anaesthesia in the studied fish, where the anaesthesia induction time decreased with increased OEO concentrations. Meanwhile, the inverse was reported for recovery time. Based on the ideal anaesthetic criteria, 50 μL L−1 and 100 μL L−1 were the suitable concentrations that could be recommended for quick anaesthesia. However, according to the haemato-biochemical parameters and survival results, 25 μL L−1 was safe to anaesthetise tilapia mossambicus for 10 min and could be recommended for time-consuming fish-handling procedures. Future studies should investigate multiple factors that influence anaesthesia in fish for better optimisation of OEO in tilapia mossambicus.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"12 6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82611626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Black Soldier Fly Larvae (Hermetia illucens) as Dietary Replacement of Extruded Feed for Tambaqui (Colossoma macropomum) Juveniles","authors":"B. Ordoñez, T. Santana, Davison Carneiro, Driely dos Santos, G. Parra, Luis Moreno, N. Teixeira Filho, F. Aguilar, F. Yamamoto, L. Gonçalves","doi":"10.3390/aquacj2040014","DOIUrl":"https://doi.org/10.3390/aquacj2040014","url":null,"abstract":"The black soldier fly (BSF, Hermetia illucens) is a prominent insect species and a novel alternative ingredient for aquafeeds. This study aimed to evaluate the replacement of commercial feed with fresh whole black soldier fly larvae (BSFL) for Colossoma macropomum. Juvenile tambaqui (115.2 ± 0.9 g/fish) were stocked in 800 L tanks (11 fish/tank) operating as a recirculating system. The dietary treatments consisted of whole larvae only (BSFL), 50:50 BSFL: commercial feed (BSFL: CF), or only commercial feed (CF), and the fish were fed to apparent satiation for 120 days. At the end of the feeding trial, fish were group weighed, and blood and muscle were collected for hematological and sensorial evaluations. Fish fed BSFL:CF presented with similar growth performance and carcass yield to tambaqui that were fed only CF. The high fat content of the larvae contributed to the highest values for the viscerosomatic index (7.01%; 6.56%), plasmatic triglycerides (312.1 mg/dL; 295.1 mg/dL), and cholesterol (120.6 mg/dL; 119.1 mg/dL) in the fish provided with BSFL or BSFL:CF, respectively. However, a better acceptance in the fillet sensory analysis was observed for these fillets than for the fillets from the CF group. Feeding tambaqui with BSFL:CF did not compromise the production performance and may have improved the acceptance of their fillets.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76321122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth and Welfare of African Catfish (Clarias gariepinus Burchell, 1822) under Dietary Supplementation with Mixed-Layer Clay Mineral Montmorillonite-Illite/Muscovite in Commercial Aquaculture","authors":"H. Palm, Erwin Berchtold, Benedikt Gille, U. Knaus, L. Wenzel, Björn Baßmann","doi":"10.3390/aquacj2030013","DOIUrl":"https://doi.org/10.3390/aquacj2030013","url":null,"abstract":"Juvenile African catfish (Clarias gariepinus Burchell, 1822) were reared within two experiments (a research facility and a local catfish farm, E1 and E2, respectively) for 102 d each under commercial recirculating aquaculture conditions. The mixed-layer clay mineral montmorillonite–illite/muscovite (1g557) was applied as a feed additive at concentrations of 0.5% and 2.0%, which were compared with an unsupplemented control (0.0%) over 70 d. For E1, feeding was automatic at night, while E2 was fed manually during the day. The growth and physiological welfare parameters of the fish were monitored, including the mortality, skin lesions, stress responses after confinement (plasma cortisol and glucose), and additional blood parameters. Tendentially, the most efficient growth in both the experiments was observed in the 0.5% groups, which performed slightly better than the controls (E1: 0.8% and E2: 3.2%) despite a lower nutrient content (p > 0.05). In E1, the negative skewness of the leptokurtic distribution also revealed the highest number of larger-sized fish per batch. Mortality was low in all the treatment groups (E1 control/0.5%/2.0%: 3.6%/4.9%/2.9%; E2 control/0.5%: 2.6%/5.5%). After only 29 d in E1, the number of skin lesions per fish decreased significantly (p < 0.05 between each of the 0.5% and 2.0% groups, compared to the control (E1 control/0.5%/2.0%: 1.2/0.8/0.8). In both E1 and E2, the number of lesions per fish decreased even further after 70 d, significantly between the treatment groups and the control (E1 control/0.5%/2.0%: 0.9/0.4/0.5 and E2 control/0.5%: 0.6/0.3). In E1, the cortisol and glucose concentrations increased strongly in all the groups due to the induced stress, whereas this was not evident in E2 based on the different sampling procedure. The additional blood parameters (aspartate aminotransferase, glutamate dehydrogenase, urea, calcium, phosphate, total protein, leucocytes, erythrocytes, hematocrit, cholesterol, triglycerides, sodium, potassium, and chloride) revealed no significant difference between the treatment groups in either experiment, indicating no negative effects of 1g557 on the organs or metabolism of the fish. Supplementation with 0.5% 1g557 in the common commercial feeds for African catfish increases growth performance (p > 0.05), reduces size variance, and supports fish welfare under different commercial aquaculture conditions in the present study.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76750959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Poultry Corn By-Product Meal on the Growth Performance of Channel Catfish, Ictalurus punctatus","authors":"S. Nazeer, Ashley Fredricks, O. E. Afe, B. J. Bench, Zach Thompson, D. Davis","doi":"10.3390/aquacj2030012","DOIUrl":"https://doi.org/10.3390/aquacj2030012","url":null,"abstract":"In this study, three growth trials were conducted to check the efficacy of poultry corn by-product meal, which was a combination of wet poultry processing waste and corn that was co-dried. It was relatively a new product, and its first growth trial was conducted in a laboratory (aquarium study) to evaluate the substitution of whole corn meal with poultry plus corn by-product meal (PCBM) in practical diets for channel catfish. In this trial (84 days culture period), 7 levels of PCBM (0, 5, 10, 15, 20, 25 and 30%) were evaluated in a practical diet containing 32% protein and 6% lipid. The results indicated that fish fed PCBM20 had the highest FW, WG and WG% among all the treatments, and these values were significantly higher (p < 0.05) than at baseline or with PCBM5 treatment. The second trial (fingerlings to sub-market) was conducted in 12 raceways with 4 levels of PCBM (0, 10, 20 and 30%) and 600 fish (mean initial weight 42.3 ± 5.06 g) in each raceway. After 143 days of culture, the results demonstrated that there were no significant differences (p > 0.05) between the FW, WG and WG% and the survival of the fish. To validate our results again, the third trial (sub-market to market) was conducted in 6 raceways with 2 levels of PCBM (0 and 30%). A total of 600 fish (mean initial weight 136.8 ± 6.3 g) were stocked in each raceway. The results revealed that there were no significant differences (p > 0.05) between the FW, WG and WG% and fish survival after the culture period of 133 days. In all three trials, upon termination, the hepatosomatic index (HIS), the intraperitoneal fat (IPF), and the dress-out (headed and gutted) percentages were measured for trials 2 and 3. The results showed that there were no significant differences (p > 0.05) between all these parameters except for HIS in trial 1 and IPF in trial 2 (p < 0.05). In conclusion, PCBM can be used up to 30% in the diets of channel catfish.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"95 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85857858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of Nile Tilapia (Oreochromis niloticus) Farming Intensities in Liberia","authors":"Mandela Klon-Yan Hinneh, Mbeva D. Liti, G. Matolla","doi":"10.3390/aquacj2030011","DOIUrl":"https://doi.org/10.3390/aquacj2030011","url":null,"abstract":"Fish farming is a rapidly growing food sector in developing nations. Liberia is an example of a least developed country with a large population facing high poverty levels. This has led to the adoption of aquaculture as one of the most important strategies for solving malnutrition and food security problems. However, since the introduction of fish farming, fish yields have been persistently low. To address the shortcomings in fish yields in Liberia, a study was conducted to provide information on fish farming intensities, types, and quality of feeds used by farmers in the culture of O. niloticus in Bong, Lofa, Nimba, and Grande Gedeh counties. Using stratified purposive sampling, 120 farmers were interviewed, and their fish feeds were sampled for proximate nutrient analyses. The results demonstrated that fish farming of O. niloticus in Liberia is mostly semi-extensive (81.6%), mainly practiced in paddy, barrage, and earthen ponds. On average, farmers produce 165.7 kg ha−1 of O. niloticus annually, translating to USD 414.25. Farmers use mixed feeding regimes, comprising farmer-made, kitchen waste, and blended commercial feeds. Farmers, on average, spend 43% of their operation cost on feeds, which makes it unsustainable to maintain semi-intensive systems. The main feed ingredients used by Liberian fish farmers are rice bran, wheat bran, corn, palm kernel, and fishmeal. Crude protein levels in feed ingredients are as follows: rice bran (3.7 ± 1.3%), wheat bran (16.4 ± 1.5%), corn (6.3 ± 1.1%), palm kernel cake (14.8 ± 1.4%), and fishmeal (63.8 ± 1.3%). Crude proteins were low in formulated feeds, ranging from 8–15% CP. From this study, poor yields and the slow growth of O. niloticus can be attributed to low-protein diets, rendering farming ventures unprofitable and unsustainable for resource-poor farmers in Liberia.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"298 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79672710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}