Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, China

Q4 Environmental Science
Qiushui Wang, Xin‐wei Zhou, Yue Liu, Qi Ding, Zan Wu, Jie Deng, Jia Zuo, Liyan Yuan, Peng Shao, Bo Cheng, Lijuan Gao
{"title":"Bacterial Community and Antibiotic Resistance Gene Profiles of Fish Gut Contents and Their Aquaculture Environment in Tianjin, China","authors":"Qiushui Wang, Xin‐wei Zhou, Yue Liu, Qi Ding, Zan Wu, Jie Deng, Jia Zuo, Liyan Yuan, Peng Shao, Bo Cheng, Lijuan Gao","doi":"10.3390/aquacj2040016","DOIUrl":null,"url":null,"abstract":"The continuous expansion of freshwater fish culture is confronted with environmental problems owing to their high antibiotic inputs and antibiotic-resistant bacteria. This study explored the antibiotic resistance gene (ARG) profiles of water and fish gut contents from three fishponds in Tianjin using a metagenomics approach. Proteobacteria, Bacteroidetes, and Actinobacteria were the most abundant phyla in all water samples. However, the microbial composition of the fish guts was distinctly different among the three aquaculture farms. Cetobacterium, Bacillus, Weissella, and Fusobacterium were the dominant genera in the gut contents of all fish. More than 20 unique ARGs with relatively high abundances were detected in both water and fish gut content samples. The dominant genes and pathways of antibiotic resistance mechanisms detected in all samples were antibiotic efflux, antibiotic inactivation, antibiotic target alteration, antibiotic target protection, antibiotic target replacement, and reduced permeability to antibiotics. In addition, our results indicate that antibiotics, such as florfenicol, and heavy metals, such as Zn and Cu, could have a significant correlation with some common ARGs, indicating that antibiotic-resistant bacteria could co-occur with heavy metals. Our study provides a research basis for the development of a strategy for practical antibiotic application and heavy metal monitoring in aquaculture.","PeriodicalId":36566,"journal":{"name":"Indonesian Aquaculture Journal","volume":"18 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indonesian Aquaculture Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3390/aquacj2040016","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Environmental Science","Score":null,"Total":0}
引用次数: 1

Abstract

The continuous expansion of freshwater fish culture is confronted with environmental problems owing to their high antibiotic inputs and antibiotic-resistant bacteria. This study explored the antibiotic resistance gene (ARG) profiles of water and fish gut contents from three fishponds in Tianjin using a metagenomics approach. Proteobacteria, Bacteroidetes, and Actinobacteria were the most abundant phyla in all water samples. However, the microbial composition of the fish guts was distinctly different among the three aquaculture farms. Cetobacterium, Bacillus, Weissella, and Fusobacterium were the dominant genera in the gut contents of all fish. More than 20 unique ARGs with relatively high abundances were detected in both water and fish gut content samples. The dominant genes and pathways of antibiotic resistance mechanisms detected in all samples were antibiotic efflux, antibiotic inactivation, antibiotic target alteration, antibiotic target protection, antibiotic target replacement, and reduced permeability to antibiotics. In addition, our results indicate that antibiotics, such as florfenicol, and heavy metals, such as Zn and Cu, could have a significant correlation with some common ARGs, indicating that antibiotic-resistant bacteria could co-occur with heavy metals. Our study provides a research basis for the development of a strategy for practical antibiotic application and heavy metal monitoring in aquaculture.
天津市鱼类肠道内容物细菌群落、耐药基因及养殖环境分析
随着淡水鱼养殖规模的不断扩大,由于淡水鱼的抗生素投入和耐药菌的出现,面临着环境问题。本研究采用宏基因组学方法对天津市3个鱼塘的水和鱼肠道内容物的抗生素耐药基因(ARG)谱进行了研究。变形菌门、拟杆菌门和放线菌门是所有水样中含量最多的门。然而,三个养殖场的鱼类肠道微生物组成有明显差异。所有鱼类肠道内容物中的优势菌属为鲸杆菌、芽孢杆菌、威塞尔菌和梭菌。在水和鱼肠道样品中均检测到20多种丰度相对较高的独特ARGs。在所有样品中检测到的抗生素耐药机制的优势基因和途径为抗生素外排、抗生素失活、抗生素靶点改变、抗生素靶点保护、抗生素靶点替代和抗生素通透性降低。此外,我们的研究结果表明,抗生素(如氟苯尼考)和重金属(如锌、铜)与一些常见ARGs可能存在显著相关性,表明抗生素耐药菌可能与重金属共存。本研究为制定水产养殖中抗生素的实际应用和重金属监测策略提供了研究依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Indonesian Aquaculture Journal
Indonesian Aquaculture Journal Environmental Science-Water Science and Technology
CiteScore
0.50
自引率
0.00%
发文量
9
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信