Cell Surface最新文献_第9页

Characterization of Neurospora crassa GH16, GH17, and GH72 gene families of cell wall crosslinking enzymes 粗神经孢子虫细胞壁交联酶GH16、GH17和GH72基因家族的研究

Cell Surface Pub Date : 2022-12-01 DOI: 10.1016/j.tcsw.2022.100073

Pavan Patel, Stephen J. Free

{"title":"Characterization of Neurospora crassa GH16, GH17, and GH72 gene families of cell wall crosslinking enzymes","authors":"Pavan Patel, Stephen J. Free","doi":"10.1016/j.tcsw.2022.100073","DOIUrl":"10.1016/j.tcsw.2022.100073","url":null,"abstract":"<div>GH16 chitin transferases, GH17 β-1,3-glucan transferases, and GH72 β-1,3-glucan/lichenin transferases are important fungal cell wall crosslinking enzymes. The Neurospora crassa genome encodes three genes from the GH17 gene family and five members in the GH16 subfamily 18 and 19 fungal chitin transferases. We created deletion mutants lacking all three GH17 genes and determined that they had wild type morphology and are more sensitive to cell wall perturbation reagents than the wild type. We also created deletion mutants lacking all five GH16 subfamily 18 and 19 genes and found that they had wild type morphology and are more sensitive to cell wall perturbation reagents than the wild type. We conclude that GH16 and GH17 enzymes play roles in cell wall biogenesis. In N. crassa, GH72 enzymes have been reported to be lichenin transferases, while in other fungi they have been shown to be the β-1,3-glucan transferases. Neurospora triple GH72 deletions give rise to a tight colonial morphology, sensitivity to cell wall perturbation reagents, and release of cell wall proteins into the medium. To ask if GH72 and GH17 enzymes might be redundant in N. crassa, we created sextuple mutants lacking the three GH72 genes and the three GH17 genes and found that they were indistinguishable from the GH72 triple mutant. We also found that a recombinant GH72 enzyme is able to form a lichenin-enzyme intermediate demonstrating that GH72 enzymes are lichenin transferases. The N. crassa GH72 enzymes are lichenin transferases and are not redundant with the GH17 β-1,3-glucan transferases.</div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"8 ","pages":"Article 100073"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8777122/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39860109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Outer membrane-anchoring enables LpoB to regulate peptidoglycan synthesis rate 外膜锚定使LpoB能够调节肽聚糖的合成速率

Cell Surface Pub Date : 2022-12-01 DOI: 10.1016/j.tcsw.2022.100086

Ali A. Kermani , Jacob Biboy , Daniela Vollmer, Waldemar Vollmer

{"title":"Outer membrane-anchoring enables LpoB to regulate peptidoglycan synthesis rate","authors":"Ali A. Kermani , Jacob Biboy , Daniela Vollmer, Waldemar Vollmer","doi":"10.1016/j.tcsw.2022.100086","DOIUrl":"10.1016/j.tcsw.2022.100086","url":null,"abstract":"<div>Peptidoglycan (PG) is an essential component of the cell envelope in most bacteria, responsible for maintaining the shape of the cell and protecting the cell from environmental stresses. The growth of the PG layer during cell elongation and division is facilitated by the coordinated activities of PG synthases and hydrolases. PG synthases are regulated from inside the cell by components of the elongasome and divisome complexes driven by the cytoskeletal proteins MreB and FtsZ. In Escherichia coli the PG synthases PBP1A and PBP1B require the activation by outer membrane (OM)-anchored lipoproteins LpoA and LpoB, respectively. These have an elongated structure and are capable to span the periplasm to reach their cognate, cytoplasmic membrane (CM)-anchored PG synthase through the PG layer. Presumably, the Lpo proteins activate the PBPs at sites where the PG mesh is stretched or defective, resulting in coupling of PG synthase activation with cell growth or PG repair. Here we investigated the importance of OM-anchoring on the function of Lpo proteins in regulating PG synthesis in response to environmental stresses. We investigated the effects of an artificially CM-tethered LpoB on cell morphology and PG synthesis. Our results indicate that mis-localization of LpoB affects the growth and morphology of cells in high osmolarity growth medium, and PG synthesis rate upon an osmotic upshift.</div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"8 ","pages":"Article 100086"},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9593243/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40651847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Malectin/Malectin-like domain-containing proteins: A repertoire of cell surface molecules with broad functional potential Malectin/Malectin-like domains containing protein:一类具有广泛功能潜力的细胞表面分子

Cell Surface Pub Date : 2021-12-01 DOI: 10.1016/j.tcsw.2021.100056

He Yang , Dong Wang , Li Guo , Huairong Pan , Robert Yvon , Scott Garman , Hen-Ming Wu , Alice Y. Cheung

{"title":"Malectin/Malectin-like domain-containing proteins: A repertoire of cell surface molecules with broad functional potential","authors":"He Yang , Dong Wang , Li Guo , Huairong Pan , Robert Yvon , Scott Garman , Hen-Ming Wu , Alice Y. Cheung","doi":"10.1016/j.tcsw.2021.100056","DOIUrl":"10.1016/j.tcsw.2021.100056","url":null,"abstract":"<div>Cell walls are at the front line of interactions between walled-organisms and their environment. They support cell expansion, ensure cell integrity and, for multicellular organisms such as plants, they provide cell adherence, support cell shape morphogenesis and mediate cell–cell communication. Wall-sensing, detecting perturbations in the wall and signaling the cell to respond accordingly, is crucial for growth and survival. In recent years, plant signaling research has suggested that a large family of receptor-like kinases (RLKs) could function as wall sensors partly because their extracellular domains show homology with malectin, a diglucose binding protein from the endoplasmic reticulum of animal cells. Studies of several malectin/malectin-like (M/ML) domain-containing RLKs (M/MLD-RLKs) from the model plant Arabidopsis thaliana have revealed an impressive array of biological roles, controlling growth, reproduction and stress responses, processes that in various ways rely on or affect the cell wall. Malectin homologous sequences are widespread across biological kingdoms, but plants have uniquely evolved a highly expanded family of proteins with ML domains embedded within various protein contexts. Here, we present an overview on proteins with malectin homologous sequences in different kingdoms, discuss the chromosomal organization of Arabidopsis M/MLD-RLKs and the phylogenetic relationship between these proteins from several model and crop species. We also discuss briefly the molecular networks that enable the diverse biological roles served by M/MLD-RLKs studied thus far.</div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"7 ","pages":"Article 100056"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.tcsw.2021.100056","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39221429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

In vitro efficacy of relebactam versus avibactam against Mycobacterium abscessus complex 瑞巴坦与阿维巴坦抗脓肿分枝杆菌复合体的体外疗效比较

Cell Surface Pub Date : 2021-12-01 DOI: 10.1016/j.tcsw.2021.100064

James Harrison , John A. Weaver , Maya Desai , Jonathan A.G. Cox

{"title":"In vitro efficacy of relebactam versus avibactam against Mycobacterium abscessus complex","authors":"James Harrison , John A. Weaver , Maya Desai , Jonathan A.G. Cox","doi":"10.1016/j.tcsw.2021.100064","DOIUrl":"10.1016/j.tcsw.2021.100064","url":null,"abstract":"<div>Infections resulting from Mycobacterium abscessus are increasing in prevalence worldwide, with the greatest risk posed to patients with underlying respiratory conditions. Treatment for infections is difficult due to wide ranging intrinsic antimicrobial resistance, which is compounded by the existence of a range of subspecies within the M. abscessus complex, each with varying additional antimicrobial resistance profiles. Previously, the use of β-lactam/β-lactamase inhibitors within a combination therapy has been proposed as an effective treatment option for pulmonary M. abscessus infections. Here, we assess the in vitro efficacy of two non-β-lactam based inhibitors, relebactam and avibactam, as agents against M. abscessus with their respective partner drugs imipenem and ceftazidime, as well as in triplicate combinations with additional β-lactam antibiotics against the M. abscessus complex. We have shown that the commercially available ratio of imipenem to relebactam is the appropriate ratio for bactericidal activity against M. abscessus, whereas the ratio between ceftazidime and avibactam is redundant, due to inactivity of ceftazidime to inhibit the bacteria. We have identified that the use of imipenem and meropenem alongside either relebactam or avibactam yield low minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) for each M. abscessus subspecies, which are within the therapeutically achievable concentration ranges within the epithelial lining fluid of the lungs. We propose the implementation of imipenem with relebactam in place of stand-alone imipenem into the current treatment regime, alongside meropenem, as a future front-line treatment option for M. abscessus complex infections.</div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"7 ","pages":"Article 100064"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8521170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39564392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The role of pectin phase separation in plant cell wall assembly and growth 果胶相分离在植物细胞壁组装和生长中的作用

Cell Surface Pub Date : 2021-12-01 DOI: 10.1016/j.tcsw.2021.100054

Kalina T. Haas , Raymond Wightman , Alexis Peaucelle , Herman Höfte

{"title":"The role of pectin phase separation in plant cell wall assembly and growth","authors":"Kalina T. Haas , Raymond Wightman , Alexis Peaucelle , Herman Höfte","doi":"10.1016/j.tcsw.2021.100054","DOIUrl":"10.1016/j.tcsw.2021.100054","url":null,"abstract":"<div>A rapidly increasing body of literature suggests that many biological processes are driven by phase separation within polymer mixtures. Liquid-liquid phase separation can lead to the formation of membrane-less organelles, which are thought to play a wide variety of roles in cell metabolism, gene regulation or signaling. One of the characteristics of these systems is that they are poised at phase transition boundaries, which makes them perfectly suited to elicit robust cellular responses to often very small changes in the cell’s “environment”. Recent observations suggest that, also in the semi-solid environment of plant cell walls, phase separation not only plays a role in wall patterning, hydration and stress relaxation during growth, but also may provide a driving force for cell wall expansion. In this context, pectins, the major polyanionic polysaccharides in the walls of growing cells, appear to play a critical role. Here, we will discuss (i) our current understanding of the structure–function relationship of pectins, (ii) in vivo evidence that pectin modification can drive critical phase transitions in the cell wall, (iii) how such phase transitions may drive cell wall expansion in addition to turgor pressure and (iv) the periodic cellular processes that may control phase transitions underlying cell wall assembly and expansion.</div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"7 ","pages":"Article 100054"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.tcsw.2021.100054","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39244732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 39

High-resolution 3D mapping of rhizosphere glycan patterning using molecular probes in a transparent soil system 透明土壤系统中使用分子探针的根际聚糖图谱的高分辨率三维制图

Cell Surface Pub Date : 2021-12-01 DOI: 10.1016/j.tcsw.2021.100059

Catherine Y. Jones , Ilonka Engelhardt , Daniel Patko , Lionel Dupuy , Nicola Holden , William G.T. Willats

引用次数: 5

The biophysics of bacterial infections: Adhesion events in the light of force spectroscopy 细菌感染的生物物理学:力光谱下的粘附事件

Cell Surface Pub Date : 2021-12-01 DOI: 10.1016/j.tcsw.2021.100048

Paula Parreira , M. Cristina L. Martins

{"title":"The biophysics of bacterial infections: Adhesion events in the light of force spectroscopy","authors":"Paula Parreira , M. Cristina L. Martins","doi":"10.1016/j.tcsw.2021.100048","DOIUrl":"10.1016/j.tcsw.2021.100048","url":null,"abstract":"<div>Bacterial infections are the most eminent public health challenge of the 21st century. The primary step leading to infection is bacterial adhesion to the surface of host cells or medical devices, which is mediated by a multitude of molecular interactions. At the interface of life sciences and physics, last years advances in atomic force microscopy (AFM)-based force spectroscopy techniques have made possible to measure the forces driving bacteria-cell and bacteria-materials interactions on a single molecule/cell basis (single molecule/cell force spectroscopy).Among the bacteria-(bio)materials surface interactions, the life-threatening infections associated to medical devices involving Staphylococcus aureus and Escherichia coli are the most eminent. On the other hand, Pseudomonas aeruginosa binding to the pulmonary and urinary tract or the Helicobacter pylori binding to the gastric mucosa, are classical examples of bacteria-host cell interactions that end in serious infections.As we approach the end of the antibiotic era, acquisition of a deeper knowledge of the fundamental forces involved in bacteria – host cells/(bio)materials surface adhesion is crucial for the identification of new ligand-binding events and its assessment as novel targets for alternative anti-infective therapies.This article aims to highlight the potential of AFM-based force spectroscopy for new targeted therapies development against bacterial infections in which adhesion plays a pivotal role and does not aim to be an extensive overview on the AFM technical capabilities and theory of single molecule force spectroscopy.</div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"7 ","pages":"Article 100048"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.tcsw.2021.100048","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25433430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

CreA-mediated repression of gene expression occurs at low monosaccharide levels during fungal plant biomass conversion in a time and substrate dependent manner crea介导的基因表达抑制在真菌植物生物量转化过程中以低单糖水平发生，并以时间和底物依赖的方式发生

Cell Surface Pub Date : 2021-12-01 DOI: 10.1016/j.tcsw.2021.100050

Mao Peng , Claire Khosravi , Ronnie J.M. Lubbers , Roland S. Kun , Maria Victoria Aguilar Pontes , Evy Battaglia , Cindy Chen , Sacha Dalhuijsen , Paul Daly , Anna Lipzen , Vivian Ng , Juying Yan , Mei Wang , Jaap Visser , Igor V. Grigoriev , Miia R. Mäkelä , Ronald P. de Vries

{"title":"CreA-mediated repression of gene expression occurs at low monosaccharide levels during fungal plant biomass conversion in a time and substrate dependent manner","authors":"Mao Peng , Claire Khosravi , Ronnie J.M. Lubbers , Roland S. Kun , Maria Victoria Aguilar Pontes , Evy Battaglia , Cindy Chen , Sacha Dalhuijsen , Paul Daly , Anna Lipzen , Vivian Ng , Juying Yan , Mei Wang , Jaap Visser , Igor V. Grigoriev , Miia R. Mäkelä , Ronald P. de Vries","doi":"10.1016/j.tcsw.2021.100050","DOIUrl":"10.1016/j.tcsw.2021.100050","url":null,"abstract":"<div>Carbon catabolite repression enables fungi to utilize the most favourable carbon source in the environment, and is mediated by a key regulator, CreA, in most fungi. CreA-mediated regulation has mainly been studied at high monosaccharide concentrations, an uncommon situation in most natural biotopes. In nature, many fungi rely on plant biomass as their major carbon source by producing enzymes to degrade plant cell wall polysaccharides into metabolizable sugars. To determine the role of CreA when fungi grow in more natural conditions and in particular with respect to degradation and conversion of plant cell walls, we compared transcriptomes of a creA deletion and reference strain of the ascomycete Aspergillus niger during growth on sugar beet pulp and wheat bran. Transcriptomics, extracellular sugar concentrations and growth profiling of A. niger on a variety of carbon sources, revealed that also under conditions with low concentrations of free monosaccharides, CreA has a major effect on gene expression in a strong time and substrate composition dependent manner. In addition, we compared the CreA regulon from five fungi during their growth on crude plant biomass or cellulose. It showed that CreA commonly regulated genes related to carbon metabolism, sugar transport and plant cell wall degrading enzymes across different species. We therefore conclude that CreA has a crucial role for fungi also in adapting to low sugar concentrations as occurring in their natural biotopes, which is supported by the presence of CreA orthologs in nearly all fungi.</div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"7 ","pages":"Article 100050"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.tcsw.2021.100050","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25526966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Fungal cell wall components modulate our immune system 真菌细胞壁成分调节我们的免疫系统

Cell Surface Pub Date : 2021-12-01 DOI: 10.1016/j.tcsw.2021.100067

Benoit Briard , Thierry Fontaine , Thirumala-Devi Kanneganti , Neil A.R. Gow , Nicolas Papon

引用次数: 8

The multi-target aspect of an MmpL3 inhibitor: The BM212 series of compounds bind EthR2, a transcriptional regulator of ethionamide activation MmpL3抑制剂的多靶点方面:BM212系列化合物结合EthR2，乙硫酰胺激活的转录调节因子

Cell Surface Pub Date : 2021-12-01 DOI: 10.1016/j.tcsw.2021.100068

Alice R. Moorey , Alejandro Cabanillas , Sarah M. Batt , Sonja Ghidelli-Disse , Beatriz Urones , Olalla Sanz , Joel Lelievre , Marcus Bantscheff , Liam R. Cox , Gurdyal S. Besra

{"title":"The multi-target aspect of an MmpL3 inhibitor: The BM212 series of compounds bind EthR2, a transcriptional regulator of ethionamide activation","authors":"Alice R. Moorey , Alejandro Cabanillas , Sarah M. Batt , Sonja Ghidelli-Disse , Beatriz Urones , Olalla Sanz , Joel Lelievre , Marcus Bantscheff , Liam R. Cox , Gurdyal S. Besra","doi":"10.1016/j.tcsw.2021.100068","DOIUrl":"10.1016/j.tcsw.2021.100068","url":null,"abstract":"<div>The emergence of drug-resistant strains of Mycobacterium tuberculosis (Mtb) ensures that drug discovery efforts remain at the forefront of TB research. There are multiple different experimental approaches that can be employed in the discovery of anti-TB agents. Notably, inhibitors of MmpL3 are numerous and structurally diverse in Mtb and have been discovered through the generation of spontaneous resistant mutants and subsequent whole genome sequencing studies. However, this approach is not always reliable and can lead to incorrect target assignment and requires orthogonal confirmatory approaches. In fact, many of these inhibitors have also been shown to act as multi-target agents, with secondary targets in Mtb, as well as in other non-MmpL3-containing pathogens. Herein, we have investigated further the cellular targets of the MmpL3-inhibitor BM212 and a number of BM212 analogues. To determine the alternative targets of BM212, which may have been masked by MmpL3 mutations, we have applied a combination of chemo-proteomic profiling using bead-immobilised BM212 derivatives and protein extracts, along with whole-cell and biochemical assays. The study identified EthR2 (Rv0078) as a protein that binds BM212 analogues. We further demonstrated binding of BM212 to EthR2 through an in vitro tryptophan fluorescence assay, which showed significant quenching of tryptophan fluorescence upon addition of BM212. Our studies have demonstrated the value of revisiting drugs with ambiguous targets, such as MmpL3, in an attempt to find alternative targets and the study of off-target effects to understand more precisely target engagement of new hits emerging from drug screening campaigns.</div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"7 ","pages":"Article 100068"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8634040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39712048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4