外膜锚定使LpoB能够调节肽聚糖的合成速率

Q1 Immunology and Microbiology
Ali A. Kermani , Jacob Biboy , Daniela Vollmer, Waldemar Vollmer
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引用次数: 1

摘要

肽聚糖(PG)是大多数细菌细胞包膜的重要组成部分,负责维持细胞的形状并保护细胞免受环境胁迫。在细胞伸长和分裂过程中,PG合酶和水解酶的协同作用促进了PG层的生长。PG合成酶在细胞内由细胞骨架蛋白MreB和FtsZ驱动的细长体和分裂体复合物的组分调节。在大肠杆菌中,PG合成酶PBP1A和PBP1B分别需要外膜锚定脂蛋白LpoA和LpoB激活。它们具有细长的结构,能够跨越周质,通过PG层到达它们的同源细胞质膜(CM)锚定的PG合成酶。据推测,Lpo蛋白在PG网拉伸或有缺陷的部位激活PBPs,导致PG合成酶激活与细胞生长或PG修复的偶联。在这里,我们研究了om锚定对Lpo蛋白在环境胁迫下调节PG合成功能的重要性。我们研究了人工cm拴住的LpoB对细胞形态和PG合成的影响。我们的研究结果表明,LpoB的错误定位会影响高渗透压生长介质中细胞的生长和形态,并在渗透上升时影响PG的合成速度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Outer membrane-anchoring enables LpoB to regulate peptidoglycan synthesis rate

Outer membrane-anchoring enables LpoB to regulate peptidoglycan synthesis rate

Outer membrane-anchoring enables LpoB to regulate peptidoglycan synthesis rate

Outer membrane-anchoring enables LpoB to regulate peptidoglycan synthesis rate

Peptidoglycan (PG) is an essential component of the cell envelope in most bacteria, responsible for maintaining the shape of the cell and protecting the cell from environmental stresses. The growth of the PG layer during cell elongation and division is facilitated by the coordinated activities of PG synthases and hydrolases. PG synthases are regulated from inside the cell by components of the elongasome and divisome complexes driven by the cytoskeletal proteins MreB and FtsZ. In Escherichia coli the PG synthases PBP1A and PBP1B require the activation by outer membrane (OM)-anchored lipoproteins LpoA and LpoB, respectively. These have an elongated structure and are capable to span the periplasm to reach their cognate, cytoplasmic membrane (CM)-anchored PG synthase through the PG layer. Presumably, the Lpo proteins activate the PBPs at sites where the PG mesh is stretched or defective, resulting in coupling of PG synthase activation with cell growth or PG repair. Here we investigated the importance of OM-anchoring on the function of Lpo proteins in regulating PG synthesis in response to environmental stresses. We investigated the effects of an artificially CM-tethered LpoB on cell morphology and PG synthesis. Our results indicate that mis-localization of LpoB affects the growth and morphology of cells in high osmolarity growth medium, and PG synthesis rate upon an osmotic upshift.

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来源期刊
Cell Surface
Cell Surface Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
6.10
自引率
0.00%
发文量
18
审稿时长
49 days
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