Cell Surface最新文献_第3页

Top five unanswered questions in plant cell surface research 植物细胞表面研究的五大未解之谜

Cell Surface Pub Date : 2024-02-13 DOI: 10.1016/j.tcsw.2024.100121

Wout Boerjan , Vincent Burlat , Daniel J. Cosgrove , Christophe Dunand , Paul Dupree , Kalina T. Haas , Gwyneth Ingram , Elisabeth Jamet , Debra Mohnen , Steven Moussu , Alexis Peaucelle , Staffan Persson , Cătălin Voiniciuc , Herman Höfte

引用次数: 0

Ultrastructural and morphological studies on variables affecting Escherichia coli with selected commercial antibiotics 关于影响大肠埃希氏菌与选定商业抗生素的变量的超微结构和形态学研究

Cell Surface Pub Date : 2024-01-14 DOI: 10.1016/j.tcsw.2024.100120

Nur Afrina Muhamad Hendri , Nur Asyura Nor Amdan , Shelly Olevia Dounis , Norzarila Sulaiman Najib , Santhana Raj Louis

{"title":"Ultrastructural and morphological studies on variables affecting Escherichia coli with selected commercial antibiotics","authors":"Nur Afrina Muhamad Hendri , Nur Asyura Nor Amdan , Shelly Olevia Dounis , Norzarila Sulaiman Najib , Santhana Raj Louis","doi":"10.1016/j.tcsw.2024.100120","DOIUrl":"https://doi.org/10.1016/j.tcsw.2024.100120","url":null,"abstract":"<div><h3>Background</h3><p>Many studies reported the effects of antibiotic exposure on <em>E. coli</em> bacterial growth and cell modification. However, scarce descriptive information on ultrastructural effects upon exposure of commercial antibiotics.</p></div><div><h3>Methods</h3><p>This study described the morphological and ultrastructural alterations caused by selected antibiotics (amoxicillin-clavulanate, ceftriaxone, polymyxin B, colistin, gentamicin, and amikacin) that targeted cell wall, plasma membrane, and cytoplasmic density, and also proteins synthesis. We determined extracellular morphological changes of exposure through scanning electron microscopy (FESEM) and intracellular activities through transmission electron microscopy (TEM) investigation.</p></div><div><h3>Results</h3><p>FESEM and TEM micrograph of <em>E. coli</em> exposed with selected antibiotics shows ultrastructural changes in beta-lactam class (amoxicillin-clavulanate, ceftriaxone) elongated the cells as the cell wall was altered as it inhibits bacterial cell wall synthesis, polymyxin class (polymyxin B, colistin) had plasmid and curli-fimbriae as it breaking down the plasma/cytoplasmic membrane, and aminoglycoside class (gentamicin, and amikacin) reduced ribosome concentration as it inhibits bacterial protein synthesis by binding to 30 s ribosomes.</p></div><div><h3>Conclusion</h3><p>Morphological and ultrastructural alterations of <em>E. coli</em>’s mechanism of actions were translated and depicted. This study could be reference for characterization studies for morphological and ultrastructural of <em>E. coli</em> upon exposure to antimicrobial agents.</p></div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"11 ","pages":"Article 100120"},"PeriodicalIF":0.0,"publicationDate":"2024-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468233024000021/pdfft?md5=e47390b00c583a3b62ca71468a7a1a5f&pid=1-s2.0-S2468233024000021-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139503891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Knockout of eight hydroxyproline-O-galactosyltransferases cause multiple vegetative and reproductive growth defects 敲除八种羟脯氨酸- o -半乳糖转移酶会导致多种营养和生殖发育缺陷

Cell Surface Pub Date : 2023-11-25 DOI: 10.1016/j.tcsw.2023.100117

Dasmeet Kaur , Michael A. Held , Yuan Zhang , Diana Moreira , Silvia Coimbra , Allan M. Showalter

{"title":"Knockout of eight hydroxyproline-O-galactosyltransferases cause multiple vegetative and reproductive growth defects","authors":"Dasmeet Kaur , Michael A. Held , Yuan Zhang , Diana Moreira , Silvia Coimbra , Allan M. Showalter","doi":"10.1016/j.tcsw.2023.100117","DOIUrl":"https://doi.org/10.1016/j.tcsw.2023.100117","url":null,"abstract":"<div><p>Arabinogalactan-proteins (AGPs) are a family of hyperglycosylated hydroxyproline-rich cell wall proteins found throughout the plant kingdom. To date, eight Hydroxyproline-galactosyltransferases (Hyp-GALTs), named GALT2-GALT9, are known to catalyze the addition of the first galactose sugar to Hyp residues in AGP protein cores. The generation and characterization of <em>galt23456789</em> octuple mutants using CRISPR-Cas9 gene editing technology, provided strong reverse genetic evidence that AG glycans are essential for normal vegetative and reproductive growth, as these mutants demonstrated stunted growth, greatly delayed flowering and significant defects in floral organ development and morphogenesis. Compared to the lower seed set of <em>galt25789</em> quintuple mutants being more so contributed by female gametophytic defects, dramatically low seed-set of octuple mutants was largely due to impaired male reproductive function, specifically due to shorter filaments, delayed anther dehiscence, and large decreases in pollen quantity and viability. Octuple mutant pollen had severely distorted reticulate exine, tectum patterning and intine thickness. Reduced amounts of galactose and arabinose in overall lower amounts of β-Yariv precipitated AGPs illustrated how biological functions of AGPs are affected by abnormal glycosylation.</p></div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"10 ","pages":"Article 100117"},"PeriodicalIF":0.0,"publicationDate":"2023-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468233023000245/pdfft?md5=97a2b425d59c79579fff34e40dcd748e&pid=1-s2.0-S2468233023000245-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138454011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of benzothiazinone and ethambutol on the integrity of the corynebacterial cell envelope 苯并噻嗪酮和乙胺丁醇对棒状细菌包膜完整性的影响

Cell Surface Pub Date : 2023-11-10 DOI: 10.1016/j.tcsw.2023.100116

Fabian M. Meyer , Urska Repnik , Ekaterina Karnaukhova , Karin Schubert , Marc Bramkamp

{"title":"Effects of benzothiazinone and ethambutol on the integrity of the corynebacterial cell envelope","authors":"Fabian M. Meyer , Urska Repnik , Ekaterina Karnaukhova , Karin Schubert , Marc Bramkamp","doi":"10.1016/j.tcsw.2023.100116","DOIUrl":"https://doi.org/10.1016/j.tcsw.2023.100116","url":null,"abstract":"<div><p>The mycomembrane (MM) is a mycolic acid layer covering the surface of Mycobacteria and related species. This group includes important pathogens such as <em>Mycobacterium tuberculosis</em>, Corynebacterium diphtheriae, but also the biotechnologically important strain <em>Corynebacterium glutamicum</em>. Biosynthesis of the MM is an attractive target for antibiotic intervention. The first line anti-tuberculosis drug ethambutol (EMB) and the new drug candidate, benzothiazinone 043 (BTZ) interfere with the synthesis of the arabinogalactan (AG), which is a structural scaffold for covalently attached mycolic acids that form the inner leaflet of the MM. We previously showed that <em>C. glutamicum</em> cells treated with a sublethal concentration of EMB lose the integrity of the MM. In this study we examined the effects of BTZ on the cell envelope. Our work shows that BTZ efficiently blocks the apical growth machinery, however effects in combinatorial treatment with β-lactam antibiotics are only additive, not synergistic. Transmission electron microscopy (TEM) analysis revealed a distinct middle layer in the septum of control cells considered to be the inner leaflet of the MM covalently attached to the AG. This layer was not detectable in the septa of BTZ or EMB treated cells. In addition, we observed that EMB treated cells have a thicker and less electron dense peptidoglycan (PG). While EMB and BTZ both effectively block elongation growth, BTZ also strongly reduces septal cell wall synthesis, slowing down growth effectively. This renders BTZ treated cells likely more tolerant to antibiotics that act on growing bacteria.</p></div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"10 ","pages":"Article 100116"},"PeriodicalIF":0.0,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468233023000233/pdfft?md5=7e88dd37fe3df702d4e7c1c72976e025&pid=1-s2.0-S2468233023000233-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134655670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The plasma membrane – cell wall nexus in plant cells: focus on the Hechtian structure 植物细胞的质膜-细胞壁关系:以禾田结构为中心

Cell Surface Pub Date : 2023-11-06 DOI: 10.1016/j.tcsw.2023.100115

Denise S. Arico , Johanna E.M. Dickmann , Olivier Hamant , Hervé Canut

{"title":"The plasma membrane – cell wall nexus in plant cells: focus on the Hechtian structure","authors":"Denise S. Arico , Johanna E.M. Dickmann , Olivier Hamant , Hervé Canut","doi":"10.1016/j.tcsw.2023.100115","DOIUrl":"https://doi.org/10.1016/j.tcsw.2023.100115","url":null,"abstract":"<div><p>Across all kingdoms of life, cells secrete an extracellular polymer mesh that in turn feeds back onto them. This entails physical connections between the plasma membrane and the polymer mesh. In plant cells, one connection stands out: the Hechtian strand which, during plasmolysis, reflects the existence of a physical link between the plasma membrane of the retracting protoplast and the cell wall. The Hechtian strand is part of a larger structure, which we call the Hechtian structure, that comprises the Hechtian strand, the Hechtian reticulum and the Hechtian attachment sites. Although it has been observed for more than 100 years, its molecular composition and biological functions remain ill-described. A comprehensive characterization of the Hechtian structure is a critical step towards understanding this plasma membrane-cell wall connection and its relevance in cell signaling. This short review intends to highlight the main features of the Hechtian structure, in order to provide a clear framework for future research in this under-explored and promising field.</p></div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"10 ","pages":"Article 100115"},"PeriodicalIF":0.0,"publicationDate":"2023-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468233023000221/pdfft?md5=c87b0319b8722038de40ccddf678a06e&pid=1-s2.0-S2468233023000221-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92148893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Top five unanswered questions in fungal cell surface research 真菌细胞表面研究中五大未解之谜

Cell Surface Pub Date : 2023-11-03 DOI: 10.1016/j.tcsw.2023.100114

Neil A.R. Gow , Arturo Casadevall , Wenxia Fang

引用次数: 0

Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration 对锥虫诱导的血管损伤的研究揭示了间日锥虫的隔离

Cell Surface Pub Date : 2023-10-27 DOI: 10.1016/j.tcsw.2023.100113

Sara Silva Pereira , Daniela Brás , Teresa Porqueddu , Ana M. Nascimento , Mariana De Niz

{"title":"Investigation of Trypanosoma-induced vascular damage sheds insights into Trypanosoma vivax sequestration","authors":"Sara Silva Pereira , Daniela Brás , Teresa Porqueddu , Ana M. Nascimento , Mariana De Niz","doi":"10.1016/j.tcsw.2023.100113","DOIUrl":"https://doi.org/10.1016/j.tcsw.2023.100113","url":null,"abstract":"<div><p>Multiple blood-borne pathogens infecting mammals establish close interactions with the host vascular endothelium as part of their life cycles. In this work, we investigate differences in the interactions of three <em>Trypanosoma</em> species: <em>T. brucei, T. congolense</em> and <em>T. vivax</em> with the blood vasculature. Infection with these species results in vastly different pathologies, including different effects on vascular homeostasis, such as changes in vascular permeability and microhemorrhages. While all three species are extracellular parasites, <em>T. congolense</em> is strictly intravascular, while <em>T. brucei</em> is capable of surviving both extra- and intravascularly. Our knowledge regarding <em>T. vivax</em> tropism and its capacity of migration across the vascular endothelium is unknown. In this work, we show for the first time that <em>T. vivax</em> parasites sequester to the vascular endothelium of most organs, and that, like <em>T. congolense, T. vivax</em> Y486 is largely incapable of extravasation. Infection with this parasite species results in a unique effect on vascular endothelium receptors including general downregulation of ICAM1 and ESAM, and upregulation of VCAM1, CD36 and E-selectin. Our findings on the differences between the two sequestering species (<em>T. congolense</em> and <em>T. vivax</em>) and the non-sequestering, but extravasating, <em>T. brucei</em> raise important questions on the relevance of sequestration to the parasite’s survival in the mammalian host, and the evolutionary relevance of both sequestration and extravasation.</p></div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"10 ","pages":"Article 100113"},"PeriodicalIF":0.0,"publicationDate":"2023-10-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2468233023000208/pdfft?md5=8c4ffd2ad9989855b58df363f58f3d37&pid=1-s2.0-S2468233023000208-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"92116858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Alternative mechanisms of action of metallic nanoparticles to mitigate the global spread of antibiotic-resistant bacteria 金属纳米颗粒减缓抗生素耐药性细菌全球传播的替代作用机制

Cell Surface Pub Date : 2023-10-20 DOI: 10.1016/j.tcsw.2023.100112

Abayeneh Girma

{"title":"Alternative mechanisms of action of metallic nanoparticles to mitigate the global spread of antibiotic-resistant bacteria","authors":"Abayeneh Girma","doi":"10.1016/j.tcsw.2023.100112","DOIUrl":"https://doi.org/10.1016/j.tcsw.2023.100112","url":null,"abstract":"<div><p>One of the biggest issues for medical professionals and a serious global concern is the emergence of multi-drug-resistant bacteria, which is the result of the overuse or misuse of antimicrobial agents. To combat this urgent problem, new drugs with alternative mechanisms of action are continuously replacing conventional antimicrobials. Nanotechnology-fueled innovations provide patients and medical professionals with hope for overcoming drug resistance. The aim of the present work was to document the antimicrobial potential and mechanisms of action of metallic nanoparticles against bacterial pathogens. Cell wall interaction and membrane penetration, reactive oxygen species (ROS) production, DNA damage, and protein synthesis inhibition were some of the generalised mechanisms recognised in the current study. In vitro and in vivo studies demonstrated that toxicity concerns and the development of bacterial resistance against nanoparticles (NPs) harden the use of metallic NP products for the treatment of drug-resistant bacterial pathogens. Therefore, researchers across the globe should actively engage in solving the above-mentioned issues.</p></div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"10 ","pages":"Article 100112"},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49788974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of the Candida biofilm matrix in drug and immune protection 念珠菌生物膜基质在药物和免疫保护中的作用

Cell Surface Pub Date : 2023-10-09 DOI: 10.1016/j.tcsw.2023.100111

Sumita Roy, Neil A.R. Gow

引用次数: 0

Strain and temperature dependent aggregation of Candida auris is attenuated by inhibition of surface amyloid proteins 耳念珠菌的菌株和温度依赖性聚集通过抑制表面淀粉样蛋白而减弱

Cell Surface Pub Date : 2023-07-24 DOI: 10.1016/j.tcsw.2023.100110

Dhara Malavia-Jones , Rhys A. Farrer , Mark H.T. Stappers , Matt B. Edmondson , Andrew M. Borman , Elizabeth M. Johnson , Peter N. Lipke , Neil A.R. Gow

{"title":"Strain and temperature dependent aggregation of Candida auris is attenuated by inhibition of surface amyloid proteins","authors":"Dhara Malavia-Jones , Rhys A. Farrer , Mark H.T. Stappers , Matt B. Edmondson , Andrew M. Borman , Elizabeth M. Johnson , Peter N. Lipke , Neil A.R. Gow","doi":"10.1016/j.tcsw.2023.100110","DOIUrl":"https://doi.org/10.1016/j.tcsw.2023.100110","url":null,"abstract":"<div><p><em>Candida auris</em> is a multi-drug resistant human fungal pathogen that has become a global threat to human health due to its drug resistant phenotype, persistence in the hospital environment and propensity for patient to patient spread. Isolates display variable aggregation that may affect the relative virulence of strains. Therefore, dissection of this phenotype has gained substantial interest in recent years. We studied eight clinical isolates from four different clades (I-IV); four of which had a strongly aggregating phenotype and four of which did not. Genome analysis identified polymorphisms associated with loss of cell surface proteins were enriched in weakly-aggregating strains. Additionally, we identified down-regulation of chitin synthase genes involved in the synthesis of the chitinous septum. Characterisation of the cells revealed no ultrastructural defects in cytokinesis or cell separation in aggregating isolates. Strongly and weakly aggregating strains did not differ in net surface charge or in cell surface hydrophobicity. The capacity for aggregation and for adhesion to polystyrene microspheres were also not correlated. However, aggregation and extracellular matrix formation were all increased at higher growth temperatures, and treatment with the amyloid protein inhibitor Thioflavin-T markedly attenuated aggregation. Genome analysis further indicated strain specific differences in the genome content of GPI-anchored proteins including those encoding genes with the potential to form amyloid proteins. Collectively our data suggests that aggregation is a complex strain and temperature dependent phenomenon that may be linked in part to the ability to form extracellular matrix and cell surface amyloids.</p></div>","PeriodicalId":36539,"journal":{"name":"Cell Surface","volume":"10 ","pages":"Article 100110"},"PeriodicalIF":0.0,"publicationDate":"2023-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49788971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0