Obstetrics, Gynecology and Reproduction最新文献

{"title":"Thrombogenesis-related characteristics of platelets and platelet indices in pregnant COVID-19 women","authors":"N. V. Spiridonova, T. A. Gritsenko, E. F. Khurtova","doi":"10.17749/2313-7347/ob.gyn.rep.2023.429","DOIUrl":"https://doi.org/10.17749/2313-7347/ob.gyn.rep.2023.429","url":null,"abstract":"Introduction. Thrombosis emerging in COVID-19 represents one of its most common complications. The period of pregnancy is accompanied by elevated susceptibility to infectious diseases and increased risk of thrombosis.Aim: to assess time-dependent change in platelet count and platelet indices in general clinical blood test in pregnant COVID-19 women related to illness onset and developed thrombosis.Materials and Methods. A retrospective analysis of 230 case histories from pregnant COVID-19 women at the third trimester was carried out. Of these, 99 women were selected who met the inclusion criteria: COVID-19 infection laboratory-verified by polymerase chain reaction (PCR); third trimester of pregnancy; presence/absence of thrombotic complications. All patients were divided into 2 groups: the control group consisted of 94 pregnant women aged 31.5 [28.0; 35.0] years without thrombotic complications and the average gestational age at illness onset was 34.1 [30.2; 37.6] weeks; the main group included 5 patients aged 33.0 [28.5; 37.5] years and the average gestational age at illness onset was 33.2 [30.2; 37.8] weeks, with thrombotic complications emerged during the disease course: thrombosis of upper and lower extremity veins; massive pulmonary embolism; thrombosis of the splenic vein at the spleen hilum; left atrial thrombus. The following parameters were evaluated dynamically (on day 6–8, 9–11, 12–15, 16–23 and 24–72 of the disease): total platelet count, thrombocrit, mean platelet volume, platelet distribution width (PDW), large platelet count. A comparative analysis of the study data and a correlation analysis with the time from disease onset and detected thrombosis were carried out.Results. Over the entire observation period, there were noted increased RDW (16.30 [15.80; 16.50]%), average platelet volume (10.70 [9.70; 11.80] fl), and large platelet count (73.00 [59.00; 96.00]×109/L) along with platelet count (182.50 [155.00; 184.50]×109/L) tended to elevate in main vs. control group. A correlation was established between the above parameters and illness onset as well as thrombogenesis time point (p < 0.05). The beginning of developing thrombosis in main group was mainly recorded on day 12–15 after the onset. РDW increased in both groups by day 16–23 of illness, but more pronounced changes were found in main group reaching 16.50 [16.40; 16.60]% compared to control group (16.10 [15.80; 16.40]%). By day 16–23, thrombocrit in both groups positively correlated with illness onset. In addition, large platelet count tended to rise in main group remaining, however, below the normal limit: 0.13 [0.11; 0.20]% compared to control group (0.26 [0.21; 0.31]%). Large platelet count was quite high and increased daily in both groups; the average large platelet count throughout entire observation period was lower in the main (73.00 [59.00; 96.00]×109/L) vs. control group (81.00 [66.00; 102.00]×109/L), so that in the former it sharply decreased down to 55.00 [42.00; 7","PeriodicalId":36521,"journal":{"name":"Obstetrics, Gynecology and Reproduction","volume":"19 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of key miRNAs as regulatory biomarkers of gonadotropins leading to infertility in males","authors":"Noor A. Oohayyed, Mais M. Mohammed, A. Al-Rahim, R. AlChalabi, Semaa A. Shaban, Ahmed A.J. Suleiman, Н.А. Охайед, М.М. Мохаммед, А.М. Аль-Рахим, Р.Н. АльЧалаби, С.А. Шабан, А.А.Дж, Сулейман","doi":"10.17749/2313-7347/ob.gyn.rep.2023.398","DOIUrl":"https://doi.org/10.17749/2313-7347/ob.gyn.rep.2023.398","url":null,"abstract":"Introduction. Infertility is a highly fatal reproductive system disorder that affects the ability of a couple to reproduce. Over the past decades, a drastic uplift has been recorded in infertility cases among males ranging from 20 to 70 % indicating spermatogenesis impairment.Aim: to identify key microRNAs (miRNAs) as regulatory biomarkers of gonadotropins involved in dysregulation of fertility-related genes to propose potential therapeutic strategies that would combat the action of oncogenic miRNAs (oncomiRs).Materials and Methods. Interaction analysis was performed between miRNAs and fertility-related genes namely luteinizing hormone choriogonadotropin receptor (LHCGR), gonadotropin-releasing hormone receptor (GnRHR), follicle-stimulating hormone receptor (FSHR) and cystic fibrosis transmembrane conductance regulator (CFTR) to identify key miRNAs as regulatory biomarkers of gonadotropins leading to infertility in males.Results. A total of 10, 13, 31 and 18 strong and potential binding sites were predicted for miRNAs-LHCGR, miRNAs-GnRHR, miRNAs-FSHR, and miRNAs-CFTR respectively employing miRWalk (comprehensive genetic database including miRNA targets) followed by identification of 6, 18, 55 and 17 significant interactions through RNA22. Subsequently shortlisted miRNAs and messenger RNA (mRNA) regions were subjected to Vfold-Pipeline and RNAComposer individually for 3D structure prediction. Additionally molecular docking was carried out between miRNAs and mRNAs models that discovered potential and stable interactions elucidating miR-6880-FSHR(R2) as a highly stable complex with least binding affinity (-566.3) and high confidence score (0.999).Conclusion. Hence this study proposes key oncomiRs as a diagnostic biomarker and therapeutic target to bring about a promising treatment strategy against male factor infertility. However wet lab investigations are required for further validations of proposed study.","PeriodicalId":36521,"journal":{"name":"Obstetrics, Gynecology and Reproduction","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Treatment and rehabilitation for cervical cancer: the experience of patient journey management","authors":"A. A. Akavova, A. Solopova, D. Blinov, E. Achkasov, V. Galkin, D. Korabelnikov, A. Mnatsakanyan, D. Petrenko, G. K. Bykovshchenko, P. L. Khazan","doi":"10.17749/2313-7347/ob.gyn.rep.2023.461","DOIUrl":"https://doi.org/10.17749/2313-7347/ob.gyn.rep.2023.461","url":null,"abstract":"Radical surgical intervention, chemoradiotherapy and immune therapy implicated for patients with cervical cancer (CC) can result in significant morphofunctional, psychological, sexual disorders and social maladjustment. To optimize treatment and rehabilitation for CC patients, a routing approach has been established. This approach involves the patient's journey during diagnosis and treatment with well-defined timed stages. Additionally, a rehabilitation program with lifelong follow-up has been implemented. Prior to commencing therapy, undertaking rehabilitation after oncogynecological treatment during the stage spanning from diagnosis to therapy initiation contributes to enhancing the quality of life (QoL) for CC patients.","PeriodicalId":36521,"journal":{"name":"Obstetrics, Gynecology and Reproduction","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Epigenetic mechanisms of intrauterine fetal senescence: little-known effects of hyperhomocysteinemia during pregnancy","authors":"O. Bespalova, O. V. Pachuliia, A. P. Sazonova, Yu. P. Milyutina, E. A. Kornyushina, A. V. Korenevskiy","doi":"10.17749/2313-7347/ob.gyn.rep.2023.427","DOIUrl":"https://doi.org/10.17749/2313-7347/ob.gyn.rep.2023.427","url":null,"abstract":"Today, a whole body of evidence base has been formed showing the negative effect of hyperhomocysteinemia (HHC) during pregnancy on the intrauterine fetal development: risk factors associated with development of congenital neural tube defects, congenital heart defects and non-syndromic oral facial clefts. In addition, numerous studies have shown that HHC is a risk factor for serious pregnancy complications such as repeated pregnancy loss, preterm birth, preeclampsia, placental abruption, intrauterine growth restriction, gestational diabetes mellitus. At the same time, despite multiple clinical and experimental studies, the significance of HHC effects continues to support the interest to this issue. In recent years, new data were revealed about littleknown phenomena pregnancy-associated maternal HHC such as \"epigenetic fetal aging\" and \"fetal atherogenesis\". Epigenetic aging is an individual indicator of aging that captures interindividual differences at disease onset throughout life occurring during intrauterine development being reveaked as DNA hypomethylation. Moderate HHC during pregnancy causes fetal DNA hypomethylation, which, according to recent research, may represent the main mechanism underlying health in childhood and adulthood. \"Fetal atherogenesis\" is another understudied HHC effect emphasizing that the formation of atherosclerotic plaques begins not in adulthood, but in utero. A body of studies has shown that the formation of thickening and fatty streaks in vascular intima involved in the pathogenesis of atherosclerosis begins antenatally. The discovery of new aspects of negative intrauterine HHC impact on fetal development necessitates a detailed study of HHC causes as non-modifiable (genetic defects in enzyme systems), and more importantly, conditionally modifiable causes (lack of cofactors, excessive methionine consumption, medications) for their timely leveling. It is also important to study approaches to HHC correction at the preconception stage of a female reproductive function in order to correctly \"program\" fetus and newborn development.","PeriodicalId":36521,"journal":{"name":"Obstetrics, Gynecology and Reproduction","volume":"101 9","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The many faces of thrombosis in post-cardiac surgery newborn","authors":"A. A. Kupryashov, K. A. Tokmakova, D. Samuilova, D. N. Shilkin","doi":"10.17749/2313-7347/ob.gyn.rep.2023.444","DOIUrl":"https://doi.org/10.17749/2313-7347/ob.gyn.rep.2023.444","url":null,"abstract":"We present a clinical case showing the development of two episodes of postoperative thrombosis of varying mechanisms in one patient. The first episode of thrombosis in the systemic-pulmonary anastomosis was due to shear stress-induced secretion and transformation of the von Willebrand factor (vWF) in the performed anastomosis. The altered vWF/ADAMTS-13 (a disintegrin and metalloprotease with thrombospondin type 1 motif, member 13) ratio in favor of the former was accompanied by platelet adhesion and activation in the anastomotic area that resulted in its thrombosis. The second episode of thrombosis within the superior and inferior vena cava was developed in parallel with escalated infectious process and characterized not only by thrombocytopenia, but also by decreased fibrinogen concentration. In this clinical situation, resolution of the infectious process as well as the use of proper antithrombotic therapy led to patient recovery.","PeriodicalId":36521,"journal":{"name":"Obstetrics, Gynecology and Reproduction","volume":"26 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mayer–Rokitansky–Küster–Hauser syndrome","authors":"R. Adamyan, A. K. Blbulyan, R. A. Abrahamyan, L. R. Abrahamyan, G. R. Abrahamyan, G. K. Ghardyan, D. A. Blbulyan","doi":"10.17749/2313-7347/ob.gyn.rep.2023.419","DOIUrl":"https://doi.org/10.17749/2313-7347/ob.gyn.rep.2023.419","url":null,"abstract":"The Mayer–Rokitansky–Küster–Hauser (MRKH) syndrome is an instructive story not only from a historical but also a gnoseological perspective. The mechanisms of embryogenesis as well as multiple aspects for correction of this syndrome remain enigmatic and unsolved. Here, we attempted to organize and detail striking issues related to MRKH syndrome.","PeriodicalId":36521,"journal":{"name":"Obstetrics, Gynecology and Reproduction","volume":"90 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139279864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of gene polymorphism associations linked with sex hormone binding globulin level and breast cancer of various molecular biological subtypes","authors":"K. N. Pasenov, I. V. Ponomarenko, M. I. Churnosov","doi":"10.17749/2313-7347/ob.gyn.rep.2023.455","DOIUrl":"https://doi.org/10.17749/2313-7347/ob.gyn.rep.2023.455","url":null,"abstract":"Aim : to identify specific associations between genes polymorphism associated with sex hormone-binding globulin (SHBG) level and breast cancer (BC) of various molecular biological subtypes. Materials and Methods . The retrospective comparative study was conducted using specimens collected from 261 patients with BC of two molecular biological subtypes – luminal A/B (n = 153) and triple negative (n = 108) as well as 1140 women in control group. All study participants (n = 1401) underwent a molecular genetic study of four single nucleotide polymorphism (SNP) loci, which showed a relationship with circulating SHBG level in previously conducted genome-wide association study (GWAS): rs12150660 SHBG , rs10454142 PPP1R21 , rs780093 GCKR , rs17496332 PRMT6 . Results . The analysis revealed an association between SHBG SNP candidate genes and a BC risk in patients with luminal A/B subtypes and lacked significant associations between the loci assessed and triple negative BC subtype. CC female genotype of rs10454142 PPP1R21 increased a risk of luminal A/B subtypes BC by more than 2-fold (recessive model [CC vs. TC+TT]; odds ratio = 2.07; 95 % confidence interval = 1.14–3.77; p = 0.017; p perm = 0.018). This SNP is localized in functionally \"significant\" regions of the genome (enhancers/active enhancers, promoters/active promoters) and affects methylation level in several hepatocyte DNA sites [cg15846641 (chr2:48541264)].","PeriodicalId":36521,"journal":{"name":"Obstetrics, Gynecology and Reproduction","volume":"152 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135930006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of same dose varying concentration poractant alfa on outcomes in preterm infants under 32 weeks of age","authors":"A. V. Mostovoi, A. L. Karpova, S. S. Mezhinsky, N. N. Volodin","doi":"10.17749/2313-7347/ob.gyn.rep.2023.448","DOIUrl":"https://doi.org/10.17749/2313-7347/ob.gyn.rep.2023.448","url":null,"abstract":"Introduction . We proposed a hypothesis that prognosis in preterm infants may be affected by concentration of the administered surfactant preparation able to determine its viscosity and, therefore, even distribution throughout the lungs. Aim : to assess an effect of poractant alfa (PA) administered at low (40 mg/mL) vs. standard (80 mg/mL) concentration without changing recommended dosage (200 mg/kg) on outcomes of preterm infants at gestational age (GA) under 32 weeks receiving various respiratory support. Materials and Methods . A prospective randomized controlled multicenter study was conducted. A total of 325 infants under 32 weeks of GA in five perinatal centers were randomized. The inclusion criteria were met by 264 patients: required respiratory therapy, had indications for surfactant administration at birth/within the first 30 minutes of life, and informed parental consent. Patients were excluded if they had no indications for surfactant preparations at the age of the first 30 minutes of life, had chromosomal and genetic abnormalities, congenital malformations, early neonatal sepsis, or gross deviations from the study protocol. Two groups were formed and compared: Low concentration (LC) group – PA concentration was 40 mg/mL (n = 111) and Standard concentration (SC) group (control) – PA concentration was 80 mg/mL (n = 153). Additionally, we compared two subgroups with surfactant preparation administered by minimally invasive methods in spontaneously breathing infants (using LISA – a less invasive method of introducing surfactant through a thin catheter or endotracheal tube): subgroup LC – PA concentration was 40 mg/mL (n = 27) and subgroup SC (control) – PA concentration was 80 mg/mL (n = 34). Results . It was found that development of pulmonary hemorrhages in LC and SC groups was significantly less common in infants who received PA at concentration of 40 mg/mL vs. 80 mg/mL: 3.6 (4/111) % vs. 13.1 (20/153) % (p = 0.008). While comparing subgroups with minimally invasive PA administration (LISA or endotracheal tube), we found that treatment with 40 mg/mL significantly decreased total respiratory therapy duration– 142 [70.0; 219.0] hours vs. 250 [141.0; 690.0] hours (p = 0.008), incidents of bronchopulmonary dysplasia – 4.0 (1/27) % vs. 29.0 (10/34) % (p = 0.009), length of stay in neonatal intensive care unit and hospital – 8.0 [7.5; 13.0] days vs. 14.0 [8.0; 33.75] days (p = 0.014) and 38.0 [26.5; 48.5] days vs. 50.5 [36.25; 62.5] days (p = 0.014), respectively. Conclusion . PA administered at concentration of 40 mg/mL without changing the recommended dose did not aggravate nursing of preterm infants at GA under 32 weeks. Minimally invasive PA administration at concentration of 40 mg/mL, lowered risk of bronchopulmonary dysplasia, and when used in infants on mechanical lung ventilation, it lowered a risk of pulmonary hemorrhage. All the discussed findings require to be further assessed in large prospective, multicenter, randomized studies ","PeriodicalId":36521,"journal":{"name":"Obstetrics, Gynecology and Reproduction","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135617249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemotranscriptomic analysis of 7-hydroxymatairesinol-related effects on MCF7 human breast tumor cells","authors":"I. Yu. Torshin, A. N. Rubashkina, O. A. Gromova","doi":"10.17749/2313-7347/ob.gyn.rep.2023.409","DOIUrl":"https://doi.org/10.17749/2313-7347/ob.gyn.rep.2023.409","url":null,"abstract":"Introduction . Establishing the effects of anticancer drugs on the transcriptome is an important procedure in postgenomic pharmacology necessary to comprehensively assess the desired and undesirable effects of candidate drugs. Aim: to assess the effects of lignan 7-hydroxymatairesinol (7HMR) on breast tumor cells. Materials and Methods . Chemotranscriptome profiling was carried out in MCF7 cells (breast cancer cell line) after 24-hour incubation with 7HMR. The GEO (Gene Expression Omnibus) database contains samples of data from transcriptomic studies allowing to model dose-dependent compound-related effects on gene expression based on the chemograph-derived combinatorial analysis algorithms. As a result, a panel of genes with altered expression is generated, which are analyzed by the functional linkage method using the international nomenclature of Gene Ontology (GO) linked to biological roles of genes/proteins. Results . Dose-dependent effects of 7HMR on gene transcription (change in transcription by 5 % or more per 1 μmol 7HMR) were established for 3,468 out of 12,700 genes studied. 7HMR significantly reduced the expression of genes involved in maintaining cell proliferation (401 genes including those involved in telomere maintenance), protein synthesis (194 genes) and proteasomal protein degradation (70 genes), energy metabolism in tumor cells (91 genes) and chronic inflammation (148 genes). A decline in the expression of such gene groups retards the processes of proliferation and vital activity along with protecting host body from excessive inflammation. 7HMR contributed to a predominant increase in the transcription of gene groups involved in antitumor activity (more than 100 genes), including the genes involved in maintaining antitumor immunity as well as those mediating the antitumor effects of vitamin D, retinoids, and vitamin C. Conclusion . The revealed changes in gene transcription enhance 7HMR-related effects on proteome proteins and suggest the prospects for using 7HMR for effective and safe prevention and treatment of nodular mastopathy and breast cancer.","PeriodicalId":36521,"journal":{"name":"Obstetrics, Gynecology and Reproduction","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NOD-like receptors in pathogenesis of missed and spontaneous abortions","authors":"O. P. Lebedeva, V. M. Ivannikova, I. O. Zhukova, O. N. Kozarenko, O. B. Altukhova, S. P. Pakhomov, M. I. Churnosov","doi":"10.17749/2313-7347/ob.gyn.rep.2023.435","DOIUrl":"https://doi.org/10.17749/2313-7347/ob.gyn.rep.2023.435","url":null,"abstract":"Nucleotide-binding oligomerization domain-like receptors (NOD-like receptors) are cytosolic signaling receptors of innate immune cells recognizing ligands derived from bacteria, viruses, fungi and protozoa. They can initiate apoptosis and pro-inflammatory cytokines production. Meanwhile, the role of decidual NOD-like receptors in pathogenesis of early miscarriages remains unknown. Aim : to study NOD-like receptor (NOD1, NOD2, NLRP1, NLRP3, NLRC4) messenger ribonucleic acid (mRNA) expression in decidual tissue from patients with missedand spontaneous abortions compared to progressive pregnancy. Materials and Methods . NOD1, NOD2, NLRP1, NLRP3, NLRC4 and pathway protein receptorinteracting-serine/threonine-protein kinase 2 (RIP-2) mRNA expression in decidua from 34 patients with missed abortions (group I), 34 patients with spontaneous abortions (group II) and 57 women with progressive pregnancy admitted for artificial abortion (group III, control group) were analyzed by reverse transcription quantitative polymerase chain reaction (PCR) at gestational age of 6–10 weeks. Exclusion criteria were as follows: endocrine disorders, severe extragenital diseases, antiphospholipid syndrome, inherited thrombophilia, uterine malformations and fetal chromosomal abnormalities. Samples were collected by uterine abrasion. Results . It was found that mRNA expression of NOD2 was significantly higher in decidua from patients with missed and spontaneous abortions, whereas for RIP-2 (related to relevant signaling pathway) – in women with missed abortions. A moderate positive correlation between gestational age and mRNA expression for NOD2 (R = 0.48; p = 0.01) and RIP-2 (R = 0.41; p = 0.007) was observed in subjects with progressive pregnancy. In contrast, women with missed abortions showed a moderate negative correlation between body weight and mRNA expression for NOD2 (R = –0.46; p = 0.03) and RIP-2 (R = –0.51; p = 0.02) whereas spontaneous abortions was associated with moderate negative correlation between RIP-2 mRNA expression and body weight (R= –0.47; p=0.04) as well as body mass index (R= –0.48; p = 0.04) along with moderate positive correlation with age of menarche (R = 0.46; p = 0.04). However, compared with progressive pregnancy no significant differences were found in expression level form NOD1, NLRP1, NLRP3 and NLRC4 mRNA in decidua from patients with missed and spontaneous abortions. Conclusion . Elevated NOD2 mRNA expression was observed in decidua from patients with missed and spontaneous abortions compared to progressive pregnancy paralleled with upregulated RIP-2 mRNA expression in missed abortions. Finally, it was found that NOD1, NLRP1, NLRP3 and NLRC4 were not involved in pathogenesis of miscarriages.","PeriodicalId":36521,"journal":{"name":"Obstetrics, Gynecology and Reproduction","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136152261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}