Journal of Inorganic Biochemistry最新文献

{"title":"Formulation of naphthol-derived 1,2,3-triazole: A ‘turn-on’ chemosensor for Zr(IV) with anti-cancer and anti-bacterial activities","authors":"Gurjaspreet Singh ,&nbsp;Komal ,&nbsp;Anu Radha ,&nbsp;Harshbir Kaur ,&nbsp;Bhavana Rani ,&nbsp;Baljinder Singh Gill ,&nbsp;Deepanjali Baliyan ,&nbsp;Amarjit Kaur ,&nbsp;Brij Mohan","doi":"10.1016/j.jinorgbio.2025.112944","DOIUrl":"10.1016/j.jinorgbio.2025.112944","url":null,"abstract":"<div><div>On prolonged exposure, transition metals can have detrimental effects on the environment as well as humans. However, this led to the idea for the synthesis of a chemosensor for Zr(IV) detection which is a well-known hard transition metal. In this current article, we have synthesized Naphthol-derived 1,2,3-triazole (NPTZ) via click chemistry which was characterized with the help of (NMR, FT-IR) spectroscopy, TGA studies, and mass spectral analysis. The photophysical studies had shown a great chemosensitivity towards Zr(IV) with non-interference of other metal cations. In addition, NPTZ has efficient LOD values of 26 nM (absorption analysis) and 478.6 nM (emission analysis). The Stable coordination complex of NPTZ-Zr(IV) was synthesized with a stoichiometry of 1:1 by utilizing Job's plot approach. Moreover, the NPTZ also demonstrates notable biological significance, including substantial anti-cancer and anti-microbial properties. In order to this, Molecular docking was performed which showed a considerable docking score of binding energies −8.84 kcal/mol (PDB ID-2R3I) and − 7.73 kcal/mol (PDB ID–<span><span>4OZ5</span><svg><path></path></svg></span>) with high values of inhibition constants.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"270 ","pages":"Article 112944"},"PeriodicalIF":3.8,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144068774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, characterization and glucose-lowering studies of air-stable, mixed-ligand vanadium(III) acetylacetonates and oxidovanadium(IV) complexes containing pyridine-based ligands.","authors":"Savaridasson Jose Kavitha ,&nbsp;Krishnaswamy Panchanatheswaran ,&nbsp;Mark R.J. Elsegood ,&nbsp;Sophie H. Dale ,&nbsp;Violet G. Yuen ,&nbsp;John H. McNeill","doi":"10.1016/j.jinorgbio.2025.112929","DOIUrl":"10.1016/j.jinorgbio.2025.112929","url":null,"abstract":"<div><div>The glucose-lowering properties of five acetylacetonate complexes of vanadium(III) containing picolinate (pyridine-2-carboxylate)/1,10-phenanthroline/2,2′-bipyridyl as co-ligands and three oxidovanadium(IV) complexes have been examined by <em>in vivo</em> experiments on the streptozotozin (STZ)-diabetic rat model and compared against the benchmark compound, bis(maltolato)oxidovanadium(IV). The bipyridyl and the picolinate complexes exhibit more significant activities than the phenanthroline complexes. The single crystal X-ray structures of three new complexes (acetylacetonato)bis(pyridine-2-carboxylato)vanadium(III), bis(methanol)(pyridine-2,6-dicarboxylato)oxidovanadium(IV) and (acetylacetonato)(1,10-phenanthroline)(thiocyanato)oxidovanadium(IV) are established in this work. The syntheses, spectral and electrochemical properties of all the complexes are discussed.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"270 ","pages":"Article 112929"},"PeriodicalIF":3.8,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of O2 and NO in flavodiiron proteins - Tuning the energy landscape by second sphere ligation variations","authors":"Margareta R.A. Blomberg ,&nbsp;Pia Ädelroth","doi":"10.1016/j.jinorgbio.2025.112943","DOIUrl":"10.1016/j.jinorgbio.2025.112943","url":null,"abstract":"<div><div>Flavodiiron proteins (FDPs) constitute a large family of non-heme iron enzymes present in all domains of life. They play important roles as scavengers and detoxifiers by efficiently reducing both O<span><math><msub><mrow></mrow><mn>2</mn></msub></math></span> and NO. The primary ligands of the diiron active site in all FDPs are highly conserved, indicating that the basic reaction mechanisms for O<span><math><msub><mrow></mrow><mn>2</mn></msub></math></span> and NO reduction, respectively, are the same. However, the reduction activity varies significantly between different FDPs. By comparing FDPs from two different species, <em>Thermotoga maritima</em> and <em>Desulfovibrio gigas</em>, we investigate to what extent variations in the second sphere ligation can explain differences in reduction activities. Comparisons are also made between wildtype and two variants of <em>Thermotoga maritima</em> FDP. We use Density functional theory (DFT) calculations on a number of FDP active site models to study the reaction mechanisms for both O<span><math><msub><mrow></mrow><mn>2</mn></msub></math></span> and NO reduction. For reduction of O<span><math><msub><mrow></mrow><mn>2</mn></msub></math></span> we conclude that differences in activity cannot be explained by differences in the first or second active site coordination spheres, which is mainly due to a low barrier for O<img>O bond cleavage after one proton-coupled reduction step. For NO reduction however, the rate-limiting barrier for N<span><math><msub><mrow></mrow><mn>2</mn></msub></math></span>O formation, a hyponitrite rotation, is high enough to be involved in the overall rate limitation. We show that second sphere residues, such as Tyr26 in <em>Desulfovibrio gigas</em> FDP, that can form hydrogen bonds to the rotating hyponitrite, decrease the barrier. Differences in NO reduction rate among different FDPs are most likely determined by the variation in such second sphere residues.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"270 ","pages":"Article 112943"},"PeriodicalIF":3.8,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ruthenium(II) arene complexes bearing 1,10-phenanthroline substituted with an imidazolium salt: Synthesis, and cytotoxic activities","authors":"Sinem Çakır ,&nbsp;Harika Atmaca ,&nbsp;Süleyman Ilhan ,&nbsp;Hayati Türkmen","doi":"10.1016/j.jinorgbio.2025.112937","DOIUrl":"10.1016/j.jinorgbio.2025.112937","url":null,"abstract":"<div><div>Researchers are increasingly focusing on developing target-specific, highly cytoselective, lipophilic, water-soluble Ru(II) arene complexes to mitigate the side effects of commercially available platinum-based anticancer drugs. In this context, we present novel Ru(II) arene complexes, (<strong>Ru1</strong> and <strong>Ru1a-f</strong>), which are based on a 1,10-phenanthroline-substituted imidazolium core derivatized with alkyl (butyl(<strong>a</strong>), octyl(<strong>b</strong>), dodecyl(<strong>c</strong>)) or benzyl ((benzyl(<strong>d</strong>), 2,4,6-trimethylbenzyl(<strong>e</strong>), pentamethylbenzyl(<strong>f</strong>)) groups. The structures of these complexes were characterized using ¹H, ¹³C, ¹⁹F and, ³¹P nuclear magnetic resonance (NMR) spectroscopy, Fourier transform infrared spectroscopy, mass spectrometry and elemental analysis. The cytotoxic activities of <strong>Ru1</strong> and <strong>Ru1a-f</strong> complexes were tested against the cancer cell lines MCF-7 and MDA-MB-231 and normal cell lines, such as MCF-10 A. The cell cycle distribution in the MCF-7 and MDA-MB-231 breast cancer cell lines after 72 h of incubation with IC₅₀ concentration of the complex <strong>Ru1c</strong> can validly inhibit cell growth in the G2/M phase. Flow cytometry analysis showed that the complex <strong>Ru1c</strong> induced apoptosis in MCF-7 and MDA-MB-231 breast cancer cells. Additionally, the binding mode of the complex <strong>Ru1c</strong> with Fish-Salmon DNA was examined using ultraviolet-visible spectroscopy. Interaction of <strong>Ru1c</strong> complex with bovine serum albumin was analyzed by absorption study. The stability of <strong>all complexes</strong> in the solvent was assessed using ¹H NMR spectroscopy. Additionally, quantitative determination of the total ruthenium level within the cells was performed by inductively coupled plasma mass spectrometry (ICP-MS). Molecular docking was performed to evaluate the interaction residues and docking scores of <strong>Ru1c</strong> and the reference drug cis‑platinum against CDK1, cyclin B1, Bcl-xL and Bcl-2 proteins.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"270 ","pages":"Article 112937"},"PeriodicalIF":3.8,"publicationDate":"2025-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143911599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, characterization, and microbiological evaluation of new triazolopyrimidine-based ferrocenes as potent antimicrobial prospects","authors":"Sayali Kamble ,&nbsp;Deep Rohan Chatterjee ,&nbsp;Kakad Shivani Arjun ,&nbsp;Saumya Kapoor ,&nbsp;Madhav Jadhav ,&nbsp;Shivam Gupta ,&nbsp;Moumita Ghosh Chowdhury ,&nbsp;Rudradip Das ,&nbsp;Pranav Ravindra Kombe ,&nbsp;Sapan Borah ,&nbsp;Amit Shard","doi":"10.1016/j.jinorgbio.2025.112942","DOIUrl":"10.1016/j.jinorgbio.2025.112942","url":null,"abstract":"<div><div>Antimicrobial metallodrugs have gained considerable attention for their potent inhibitory activity and clinical success, driving the development of novel metallodrug candidates. These efforts have uncovered new bioactive scaffolds and mechanisms of action. However, the global challenge of antimicrobial resistance (AMR), fueled by the genetic adaptability of microbes and resistance to nearly all antibiotic classes, highlights the urgent need for innovative antibiotics. In this study, we expand the repertoire of metallodrugs by designing, synthesizing, and biologically evaluating <strong>triazolopyrimidine-based ferrocenes</strong> as antimicrobial agents. These compounds demonstrated broad-spectrum activity against both bacterial and fungal pathogens. Advanced characterization techniques, including NMR, HRMS, FE-SEM, and SCXRD, confirmed their structural integrity and properties. Notably, the ferrocenes exhibited potent antifungal activity against <em>Candida</em> species, comparable to fluconazole, and were effective against <em>Escherichia coli</em> and <em>Staphylococcus aureus</em>. Our findings reveal a new class of metallodrugs with significant antimicrobial potential, offering promising avenues to combat AMR in future.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"270 ","pages":"Article 112942"},"PeriodicalIF":3.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Positively charged residues play a significant role in enhancing the antibacterial activity of calcitermin” [Journal of Inorganic Biochemistry, 262 (2025), 112761]","authors":"Silvia Leveraro ,&nbsp;Maria D'Accolti ,&nbsp;Erika Marzola ,&nbsp;Elisabetta Caselli ,&nbsp;Remo Guerrini ,&nbsp;Magdalena Rowinska-Zyrek ,&nbsp;Maurizio Remelli ,&nbsp;Denise Bellotti","doi":"10.1016/j.jinorgbio.2025.112940","DOIUrl":"10.1016/j.jinorgbio.2025.112940","url":null,"abstract":"","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"269 ","pages":"Article 112940"},"PeriodicalIF":3.8,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959820","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel organoruthenium complexes containing β-Diketonates: Synthesis, characterization, DNA/HSA interactions, and the impact of biocompatible ionic liquids on biological activities","authors":"Nenad Janković ,&nbsp;Goran A. Bogdanović ,&nbsp;Nevenka Gligorijević ,&nbsp;Lazar Milović ,&nbsp;Milica Međedović ,&nbsp;Jovana Matić ,&nbsp;Marijana Kosanić ,&nbsp;Milan Vraneš ,&nbsp;Ana Rilak Simović","doi":"10.1016/j.jinorgbio.2025.112941","DOIUrl":"10.1016/j.jinorgbio.2025.112941","url":null,"abstract":"<div><div>In order to discover new dual-active agents, novel ruthenium (η⁶-<em>p-</em>cymene) complexes of the general formula [(η⁶-<em>p</em>-cym)Ru(<em>O<img>O</em>)Cl] with <em>O,O</em>-diketo ester ligands ethyl 2-hydroxy-4-aryl-4-oxobut-2-enoate (<strong>1–3</strong>), were synthesized. The complexes <strong>1–3</strong> were characterized by spectral techniques (UV–Vis, IR, ¹H and ¹³C NMR, and ESI-HRMS), elemental analysis, and X-ray crystallography. Based on <em>in vitro</em> DNA/HSA experiments, complex <strong>1</strong> exhibited the highest DNA/HSA-activity, suggesting that the presence of an alkene chain contributes to increased activity. The cytotoxic activity of <strong>1</strong>–<strong>3</strong> was evaluated in a panel of human cancer cell lines (A549, MDA-MB-231, LS-174, HeLa), and in one normal cell line (MRC-5), both in the absence and presence of biocompatible ionic liquids (BIO-ILs) such as cholinium glycinate (Cho-Gly), cholinium β-alaninate (Cho-Ala), and cholinium glutamate (Cho-Glu). Complex <strong>1</strong> exhibited the highest cytotoxicity and demonstrated selectivity toward HeLa cells. Additionally, its cytotoxicity was enhanced when combined with the BIO-ILs Cho-Gly and Cho-Ala. This study suggests that ionic liquids can influence the efficacy and selectivity of cancer treatments, highlighting the potential for enhancing therapeutic outcomes. However, it also emphasizes the need for a deeper understanding of BIO-IL interactions with cellular processes. Furthermore, compound <strong>1</strong> displayed strong antimicrobial activity against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> (MIC = 0.078 mg/mL). Among the assessed species, <em>Candida albicans</em> showed the highest sensitivity to antifungal activity. These results suggest that investigated compounds may have potential for further development as clinical candidates, pending additional studies.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"270 ","pages":"Article 112941"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918464","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis, structure, stability, lipophilicity and insulin-sensitizing activity of new heteroleptic oxidovanadium(V) complexes","authors":"Sazida Yasmin Sultana ,&nbsp;Mitu Sharma ,&nbsp;Hiya Talukdar ,&nbsp;Gangutri Saikia ,&nbsp;Tahshina Begum ,&nbsp;Archana Sinha ,&nbsp;Subrata Mishra ,&nbsp;Bipul Sarma ,&nbsp;Suman Dasgupta ,&nbsp;Nashreen S. Islam","doi":"10.1016/j.jinorgbio.2025.112939","DOIUrl":"10.1016/j.jinorgbio.2025.112939","url":null,"abstract":"<div><div>A series of mixed-ligand oxidovanadium(V) complexes of the type, [VO₂(L) (N-N)] (<strong>1</strong>–<strong>4</strong>) featuring hydroxypyrones (L: maltol or ethyl-maltol) and (N-N): diimine [2,2′-bipyridine (bpy) or 1,10-phenanthroline (phen)] ligands, are reported. The synthesized complexes were characterized by spectroscopic and analytical techniques (FTIR, UV–Vis, ⁵¹V NMR, HRMS, ICP-OES and TGA). Single crystal X-ray crystallography revealed the O₄N₂ ligand sphere to define a distorted octahedral coordination geometry in each case. Each of the complexes is stable in air in the solid state and have good solubility in water as well as in organic solvents. The partition co-efficient, log <em>P</em> (octanol-water) values for the complexes being in the range (0.72–1.12) indicated their lipophilic nature. The complexes <strong>1</strong>–<strong>4</strong>, along with two previously reported complexes [VO₂(deferiprone)(bpy)]·H₂O (<strong>5</strong>) and [VO₂(deferiprone)(phen)]·4H₂O (<strong>6</strong>) were examined for their <em>in vitro</em> insulin-sensitizing and insulin-like activities against insulin responsive L6 myoblast cells. The complex, [VO₂(Emal)(bpy)]₂·H₂O (<strong>3</strong>) exhibited the most pronounced insulin-sensitizing effect, which is comparable to that of the reference compound bis(maltolato)oxidovanadium(IV), BMOV. The <em>in vitro</em> cytotoxicity assay against the L6 myoblast cells showed that the compounds were less toxic compared to BMOV. The complexes <strong>1</strong>–<strong>6</strong> were screened for their <em>in vitro</em> inhibitory effect on the model enzyme wheat thylakoid acid phosphatase (ACP). The enzyme kinetic analysis revealed that, compounds induce their inhibitory effect <em>via</em> distinct pathways. The complexes <strong>1</strong>–<strong>4</strong> served as mixed type of inhibitor (<em>K</em>_ii &gt; <em>K</em>_i), whereas <strong>5</strong> and <strong>6</strong> served as classical non-competitive inhibitors of the enzyme (<em>K</em>_ii ≈ <em>K</em>_i).</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"270 ","pages":"Article 112939"},"PeriodicalIF":3.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Harnessing redox reactions for anticancer effects: A copper(II) Schiff base complex induces apoptosis in HepG2 liver cancer cells via ROS generation","authors":"Daniela Ganci ,&nbsp;Luisa D'Anna ,&nbsp;Giulia Abruscato ,&nbsp;Malo Le Chevalier ,&nbsp;Océane Quideau ,&nbsp;Salvatore Cataldo ,&nbsp;Alberto Pettignano ,&nbsp;Simona Rubino ,&nbsp;Roberto Chiarelli ,&nbsp;Giampaolo Barone ,&nbsp;Claudio Luparello ,&nbsp;Riccardo Bonsignore","doi":"10.1016/j.jinorgbio.2025.112938","DOIUrl":"10.1016/j.jinorgbio.2025.112938","url":null,"abstract":"<div><div>This study uncovers the potential of a copper(II) Schiff base complex, CuL²⁺, to access the Cu(I) oxidation state and generate reactive oxygen species (ROS), highlighting its significance in eventual therapeutic applications. UV–vis absorption spectroscopy was used to follow the redox stability of the metal complex, also in the presence of reducing agents, such as ascorbic acid and glutathione, and of the copper(I) chelator, bathocuproine disulfonate. Utilizing human tumor cell lines HepG2 (hepatocarcinoma cells), we assessed its efficacy in reducing cell viability, increasing the sub-G₀/G₁ cell fraction, and initiating apoptotic pathways. Cell viability assays demonstrated a dose-dependent cytotoxicity with pronounced effects at sub-micromolar concentrations. Flow cytometry revealed significant ROS production, followed by mitochondrial membrane potential dissipation, and caspase activation, underscoring CuL²⁺’s mechanism of action. These findings position CuL²⁺ as a promising candidate for cancer therapy, providing insights into copper complexes' therapeutic application through oxidative stress and apoptosis modulation.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"270 ","pages":"Article 112938"},"PeriodicalIF":3.8,"publicationDate":"2025-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143924920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"NRVS of FeS cluster proteins & models – A bestiary of nifty normal modes","authors":"Hongxin Wang ,&nbsp;Vladimir Pelmenschikov ,&nbsp;Yoshitaka Yoda ,&nbsp;Stephen P. Cramer","doi":"10.1016/j.jinorgbio.2025.112935","DOIUrl":"10.1016/j.jinorgbio.2025.112935","url":null,"abstract":"<div><div>Iron‑sulfur clusters are the primordial prosthetic groups for living systems, and they have even been proposed as partly responsible for the origin of life. They play a role in essential biological processes such as electron transfer, enzyme catalysis, DNA replication and repair, small molecule sensing, iron homeostasis, apoptosis, and human health and disease. They have frequently been studied by resonance Raman, electron paramagnetic resonance, and Mössbauer spectroscopies. Over the past two decades, we have used a synchrotron method called <strong>N</strong>uclear <strong>R</strong>esonance <strong>V</strong>ibrational <strong>S</strong>pectroscopy (NRVS) to examine the vibrational dynamics of a wide variety of Fe<img>S clusters in model systems and native proteins, ranging in complexity from single Fe sites in small rubredoxins to the [7Fe-9S-C-Mo-R-homocitrate] cluster in nitrogenases.</div></div>","PeriodicalId":364,"journal":{"name":"Journal of Inorganic Biochemistry","volume":"270 ","pages":"Article 112935"},"PeriodicalIF":3.8,"publicationDate":"2025-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144134608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}