Yaoxue Xuebao Pub Date : 2016-12-01

Zhong-qi Cai, Wan-peng Li, Xi Chen, Chao Zhu, Xiao-ting Xie, Li Liu, Ying Zhao, Yan Huang, Yang Li

{"title":"[The reverse effects of allitridum on sodium current decrease caused by SCN5A-F1473S mutation].","authors":"Zhong-qi Cai, Wan-peng Li, Xi Chen, Chao Zhu, Xiao-ting Xie, Li Liu, Ying Zhao, Yan Huang, Yang Li","doi":"","DOIUrl":"","url":null,"abstract":"This study was designed to test the allitridum (All) activity in correction of sodium current decrease caused by SCN5A-F1473S mutation in HEK293 cells. The result may provide a theoretical basis for screening of new drugs in the treatment of Brugada syndrome. We transferred SCN5A-F1473S channel plasmids into HEK293 cells in a transient transfection. All was administrated acutely and chronically using extracellular irrigation flow and co-culture model. The concentration of All was 30 μmol·L(-1). We used whole cell patch clamp technique in voltage clamp mode to record current and gating kinetics. In order to explore the rescue function of All on decreased sodium peak current, we used confocal microscopy and Western blot to detect the expression of channel proteins in the cell membrane. We found a significant increase in sodium peak current of the 30 μmol·L(-1) All HEK293 cells (269.8 ± 16.6 pA/pF, P < 0.01), almost closed to the current density of the control group（298.2 ± 17.5 p A/p F, P < 0.01）. All allowed the steady-state inactivation of the channel to move toward a more positive direction (V(1/2, inact) returns to -79.5 ± 2.4 mV, P < 0.01). It also slowed the intermediate state inactivation of the channel (inactivation prolongated to 598.1 ± 22.6 ms, P < 0.01). Meanwhile, All increased distribution and expression of the channel protein in the cell membrane (compared to F1473S, P < 0.01). All caused an increase of current in SCN5A-F1473S mutation cells. We consider that the main mechanism may be related to the reduced channel inactivation by the drug with an improvement of the migration barrier of the mutational channel.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"51 12","pages":"1852-7"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36228463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Effect of endophytic fungus on expression of key enzyme genes in pathway of salidroside biosynthesis in Rhodiola crenulata]. [内生真菌对红景天红景天苷生物合成途径关键酶基因表达的影响]。

Yaoxue Xuebao Pub Date : 2016-12-01

Meng-liang Wang, Ya-nan Wang, Jin-long Cui, Jun-hong Wang

{"title":"[Effect of endophytic fungus on expression of key enzyme genes in pathway of salidroside biosynthesis in Rhodiola crenulata].","authors":"Meng-liang Wang, Ya-nan Wang, Jin-long Cui, Jun-hong Wang","doi":"","DOIUrl":"","url":null,"abstract":"One strain of endophytic fungus ZPRa-R-1 was obtained for the capacity of promoting production of salidroside in Rhodiola crenulata. To explain the mechanism of salidroside biosynthesis in host plant, eight housekeeping genes were evaluated, and the evaluation method was created for the expression activities of four key enzyme genes PAL (phenylalanine ammonia-lyase), TyDC (tyrosine decarboxylase), TAT (tyrosine transaminase), UDPGT (UDP-glucosyltransferase) referenced double reference genes in biosynthesis pathway of salidroside in R. crenulata. Stabilities of housekeeping genes were confirmed by real-time fluorescent quantitative PCR technology and three softwares including geNorm, NormFinder and BestKeeper, then relative expressions of key enzyme genes were analysized by the 2-ΔΔCt method. The results showed that the most stable gene was GAPDH, followed by PCS, and the most appropriate reference of internal genes were combination with two genes in R. crenulata inoculated with endophytic fungus ZPRa-R-1. Under symbiosis conditions, regularity changes of key enzyme genes affected by endophytic fungus ZPRa-R-1 were as follows: the relative expression activity of PAL attached to peak value, which was 4.9 times as that of control group when inoculated ten days. The relative expression of TyDC reached the maximum value, which was 2.8 times of that control after inoculating 12 days. The relative expression of UDPGT actually reach 17.1 times than that of control after inoculating 8 days. However, the relative expression of TAT was not affected by this fungus. The changes of four key enzyme genes are positively correlated with the changes of salidroside content in R. crenulata.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"51 12","pages":"1920-5"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36241362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Research progress of antipsychotics]. [抗精神病药物研究进展]

Yaoxue Xuebao Pub Date : 2016-12-01

Fei-pu Yang, Yang He, Zhen Wang, Yu Wang, Xiang-rui Jiang, Jing-shan Shen

引用次数: 0

[Operational models of allosteric modulation of G protein-coupled receptors]. G蛋白偶联受体变构调节的操作模型。

Yaoxue Xuebao Pub Date : 2016-12-01 DOI: 10.16438/J.0513-4870.2016-0775

Hao Gong, Shuo Zhang, R. Ye

引用次数: 0

[Molecular identification of wild populations of Dendrobium officinale based on target region amplification polymorphism]. 基于靶区扩增多态性的铁皮石斛野生居群分子鉴定

Yaoxue Xuebao Pub Date : 2016-12-01

Ling Liu, Rui Wu, Zhi-tao Niu, Qing-yun Xue, Wei Liu, Xiao-yu Ding

{"title":"[Molecular identification of wild populations of Dendrobium officinale based on target region amplification polymorphism].","authors":"Ling Liu, Rui Wu, Zhi-tao Niu, Qing-yun Xue, Wei Liu, Xiao-yu Ding","doi":"","DOIUrl":"","url":null,"abstract":"In this study, TRAP molecular markers were used in identification of wild populations and hybrids of Dendrobium officinale, based on the sequences of genes encoding phosphoenolpyruvate carboxylase (PEPC), UDP-glucose pyrophosphorylase (UGP) and phenylalanine ammonia-lyase (PAL). Seven polymorphic target region amplification polymorphism (TRAP) primers were selected from 54 primer combinations and used in the identification of wild populations. Moreover, hybrids had female polymorphic bands, male polymorphic bands and heterozygous bands, which suggest that seven TRAP markers are able to identify the hybrids from their parents. Furthermore, the UPGMA dendrogram revealed that when sample from Guangnan in Yunnan province was used as one parent, reciprocal hybrids grouped with them in first, and then grouped with the other parent. The results indicated that the hybrids were closer to D. officinale from Guangnan population. This study identified the wild populations and hybrids of D. officinale by TRAP molecular markers, which is useful in selection of good varieties for artificial cultivation and early identification of hybrids. The study provides a method in the control of stability of germplasm and quality of D. officinale.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"51 12","pages":"1926-33"},"PeriodicalIF":0.0,"publicationDate":"2016-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36241363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Drug glucuronidation and disposition in brain]. [药物葡萄糖醛酸化与脑内处置]。

Yaoxue Xuebao Pub Date : 2016-11-01

Zi-qian Zhang, Li Sheng, Yan Li

引用次数: 0

[Rapid characterization of constituents absorbed into blood after oral administration of steroidal saponins from Radix Ophiopogonis using UHPLC-LTQ-Orbitrap MS coupled with higher energy collision induced dissociation]. [使用UHPLC-LTQ-Orbitrap MS结合高能碰撞诱导解离快速表征口服麦冬甾体皂苷后被血液吸收的成分]。

Yaoxue Xuebao Pub Date : 2016-11-01

Ying Liu, Wei Cai, Ning Li, Si-yi Liu, Zi-jian Wang, Zhen-qing Liu, Jia-yu Zhang, Bao-sheng Zhao

引用次数: 0

[Inhibitory effect of oleanolic acid on inflammatory response in IL-1β-stimulated human synovial sarcoma SW982 cells]. 齐墩果酸对il -1β刺激的人滑膜肉瘤SW982细胞炎症反应的抑制作用。

Yaoxue Xuebao Pub Date : 2016-11-01

Jun-jiang Lian, Bin-feng Cheng, Yao-xin Gao, Ya-ping Zhang, Shuang-ping Ma, Bin-bin Zhang, Dan-dan Guo, Han Xue, Zhi-wei Feng

{"title":"[Inhibitory effect of oleanolic acid on inflammatory response in IL-1β-stimulated human synovial sarcoma SW982 cells].","authors":"Jun-jiang Lian, Bin-feng Cheng, Yao-xin Gao, Ya-ping Zhang, Shuang-ping Ma, Bin-bin Zhang, Dan-dan Guo, Han Xue, Zhi-wei Feng","doi":"","DOIUrl":"","url":null,"abstract":"To study the role of oleanolic acid on interleukin (IL)-1β-stimulated expression of inflammatory cytokines, and to explore its anti-inflammatory mechanism in SW982 cells, the toxicity of oleanolic acid on SW982 cells was detected by MTT; effects of different concentrations of oleanolic acid(5, 10, 20 μmol·L(-1)) on the expression of inflammatory factors IL-6, IL-8 and matrix metalloproteinase-1 (MMP-1) was tested at protein and m RNA levels. The study was performed in IL-1β-stimulated SW982 cells together with enzyme-linked immunosorbent assay (ELISA) and real-time fluorescence quantitative PCR (real-time PCR) methods; the influence of oleanolic acid on the phosphorylation of mitogen-activated protein kinase (MAPK), phosphatidyl inositol-3-kinase/Akt (PI3K/Akt) and nuclear transcription factor-κB (NF-κB) signaling pathways related protein was analyzed by Western blot. Results showed that different concentrations of oleanolic acid(≤40 μmol·L(-1)) were almost non-toxicity to SW982 cells; oleanolic acid significantly inhibited the expression of inflammatory factors in a dose-dependent manner; oleanolic acid restrained extracellular signal-related kinase (ERK), p38, c-jun N-terminal kinase (JNK) and Akt protein phosphorylation and IκB-α protein degradation obviously. The inhibition effect of oleanolic acid on inflammatory factors stimulated by IL-1β may be worked through MAPK, PI3K/Akt and NF-κB signaling pathways.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"51 11","pages":"1711-6"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36228012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Network pharmacology-based study on mechanisms of warming channel and relieving stagnation effects of Cinnamomum cassia Presl]. [基于网络药理学的桂树温经解郁作用机理研究]。

Yaoxue Xuebao Pub Date : 2016-11-01

Hai-dong Mu, Peng-fei Tu, Yong Jiang

{"title":"[Network pharmacology-based study on mechanisms of warming channel and relieving stagnation effects of Cinnamomum cassia Presl].","authors":"Hai-dong Mu, Peng-fei Tu, Yong Jiang","doi":"","DOIUrl":"","url":null,"abstract":"The network pharmacology gradually expands its applications to the drug development and mechanism research. The present work aims to predict the targets and the mechanisms of \"warming channel and relieving stagnation\" effects of Cinnamomum cassia Presl, especially the effect of promoting blood circulation, by means of reverse pharmacophore mapping, database mining, and some other methods. The results suggest that the main efficient components of C. cassia were procyanidins, diterpenoids, lignans, and so on, and its potential targets include fibrinogen, coagulation factor X, etc. After bioinformatics analysis, the potential influenced biological pathways were disclosed to be VEGF pathway, PDGF pathway, etc. Based on the targeted proteins and pathway forecasting workflow, a cinnamon-centered biological information network was established and the subnetwork relating to cardiovascular activity, especially antithrombotic activity, was extracted. Through this study, we establish a network pharmacology analytical guideline consisting of \"potential active components screening-target prediction-metabolic pathway analysis\" for traditional Chinese medicine, which provides a new idea and method to clarify the mechanisms of \"warming channel and relieving stagnation\" effects of C. cassia, and gives the possible directions for the following related activity and mechanism researches. Meanwhile, the method also provide a reference to others in the study of traditional Chinese medicines.","PeriodicalId":35924,"journal":{"name":"Yaoxue Xuebao","volume":"51 11","pages":"1724-33"},"PeriodicalIF":0.0,"publicationDate":"2016-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36228014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[The swelling behavior of choline fenofibrate hydrogel matrix tablets using dynamic image analysis]. 非诺贝特胆碱水凝胶基质片溶胀行为的动态图像分析

Yaoxue Xuebao Pub Date : 2016-11-01

Zhen-yun Liu, Wan-zeng Liu, He-ran Li, Zhen Li, Han Wang, Fang-zheng Lu, San-ming Li

引用次数: 0

药学学报最新文献