[使用UHPLC-LTQ-Orbitrap MS结合高能碰撞诱导解离快速表征口服麦冬甾体皂苷后被血液吸收的成分]。

药学学报 Pub Date : 2016-11-01

Ying Liu, Wei Cai, Ning Li, Si-yi Liu, Zi-jian Wang, Zhen-qing Liu, Jia-yu Zhang, Bao-sheng Zhao

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引用次数: 0

摘要

建立了UHPLC-LTQ-Orbitrap质谱联用高能碰撞解离(high - energy collision dissociation, HCD)快速分析口服麦冬甾体皂苷后血液吸收成分的方法。基于精确的质量测量、破碎模式、色谱保留时间和诊断产物离子，对31种成分进行了表征，其中包括13种糠甾醇甾体皂苷和18种螺甾醇甾体皂苷。其中8个化合物与相应的标准品相比较得到了明确的鉴定。研究结果为麦冬活性物质基础的阐明提供了全面的见解和指导。

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本刊更多论文

[Rapid characterization of constituents absorbed into blood after oral administration of steroidal saponins from Radix Ophiopogonis using UHPLC-LTQ-Orbitrap MS coupled with higher energy collision induced dissociation].

The method of UHPLC-LTQ-Orbitrap mass spectrometry coupled with higher energy collision dissociation (HCD) was established to rapidly analyze the constituents absorbed into blood after oral administration of steroidal saponins from Radix Ophiopogonis. A total of 31 constituents, including 13 furostanol steroidal saponins and 18 spirostanol steroidal saponins, were characterized based on the accurate mass measurements, fragmentation patterns, chromatographic retention times, and diagnostic product ions. Among them, 8 compounds were unambiguously identified by comparison with their corresponding standards. The results provide comprehensive insights and guidance for elucidation of material basis of Radix Ophiopogonis activity.

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来源期刊

药学学报 Pharmacology, Toxicology and Pharmaceutics-Pharmacology, Toxicology and Pharmaceutics (all)

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Acta Pharmaceutica Sinica B (APSB) is a bimonthly English peer-reviewed online journal in ScienceDirect, which publishes significant original research articles, communications and high quality reviews of recent advances. APSB encourages submissions from all areas of pharmaceutical sciences, including pharmacology, pharmaceutics, medicinal chemistry, natural products, pharmacognosy, pharmaceutical analysis and pharmacokinetics. APSB is a part of the series Acta Pharmaceutica Sinica, which was founded in 1953. The journal is co-published by Elsevier B.V., in association with the Institute of MateriaMedica, Chinese Academy of Medical Sciences and Chinese Pharmaceutical Association.