Journal of Food and Drug Analysis最新文献

{"title":"Corrigendum to \"Yi-Qi-Xuan-Fei formula ameliorate chronic obstructive pulmonary disease by remodeling lung and intestinal florase in rat models\" [J Food Drug Anal 33(3) (2025) 252-276].","authors":"","doi":"10.38212/2224-6614.3581","DOIUrl":"10.38212/2224-6614.3581","url":null,"abstract":"","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"33 4","pages":"i"},"PeriodicalIF":3.0,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12822365/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of an untargeted metabolomics analytical protocol for fecal samples by liquid chromatography-mass spectrometry.","authors":"Tsai-Wei Ting, Chih-Ning Cheng, Chieh-Chang Chen, Ching-Hua Kuo","doi":"10.38212/2224-6614.3571","DOIUrl":"10.38212/2224-6614.3571","url":null,"abstract":"<p><p>Gut microbiota has recently gained attention for its role in regulating multiple host pathways and contributing to disease developments. Fecal metabolomics using liquid chromatography-mass spectrometry (LC-MS) offers a promising approach to study gut microbial metabolites; however, it remains technically challenging due to the complex, heterogeneous nature of fecal samples and the lack of standardized protocols. This study aimed to establish a robust and reproducible untargeted fecal metabolomics workflow. We systematically evaluated sample preparation parameters-including sample amount, extraction solvent, numbers of extraction, and sample-to-solvent ratio-and assessed method reproducibility. Additionally, we compared three LC-MS data acquisition workflows using 10 samples from inflammatory bowel disease (IBD) patients and healthy controls (HC) to improve the identification of biologically relevant metabolites. In sample preparation, our results showed that 50 mg of lyophilized feces was sufficient to capture inter-individual metabolic variation. Additionally, methanol outperformed acetonitrile and showed comparable results to three binary solvent mixtures. A single extraction with methanol was sufficient, and a 1:20 (w/v) sample-to-solvent ratio maximized feature detection. Among the acquisition methods, data-dependent acquisition (DDA) with simultaneous MS1 and MS2 scans provided the highest metabolite coverage with acceptable annotation reliability. In summary, we recommend a single extraction of 50 mg lyophilized feces with 1 mL methanol and the use of DDA for sample acquisition to ensure comprehensive and reproducible untargeted analysis. This optimized protocol improves metabolite detection in human feces and offers a practical strategy to support future studies exploring gut microbial contributions to human health and disease.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"33 4","pages":"460-470"},"PeriodicalIF":3.3,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795338/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fish oil rich in ω-3 fatty acid alleviates long-term high fructose consumption-induced fatty liver and kidney dysfunction in rats.","authors":"Shing-Hwa Liu, Ruoh-Wenn Hung, Ting-Yu Chang, Meng-Tsan Chiang","doi":"10.38212/2224-6614.3573","DOIUrl":"10.38212/2224-6614.3573","url":null,"abstract":"<p><p>Excessive fructose intake leads to fatty liver and kidney dysfunction, with associated inflammation and metabolic disturbances. This study evaluated the effects of fish oil rich in ω-3 fatty acid on these conditions in rats fed a high-fructose diet. Male Sprague Dawley rats were divided into three groups: normal diet, high-fructose (HF) diet, and high-fructose diet with 5% fish oil supplementation. The experiment lasted for 21 weeks. Fish oil supplementation significantly reduced blood levels of total cholesterol, triglycerides, and advanced glycation end products (AGEs) in HF-fed rat. It also improved liver function markers and kidney function indicators. Mechanistically, fish oil suppressed fructokinase expression, activated phosphorylated AMP-activated protein kinase (p-AMPK), and decreased peroxisome proliferator-activated receptor (PPAR)-γ expression, reducing triglyceride synthesis-related proteins in the liver. In the kidneys, fish oil supplementation increased p-AMPK and PPARγ, while decreasing inflammatory and oxidative stress markers and fibrosis-related protein α-smooth muscle actin. Histological analysis confirmed that fish oil alleviated liver degeneration and kidney fibrosis. These findings suggest that fish oil not only helps regulate blood lipid levels but also alleviates fructose-induced liver and kidney damage, suggesting its potential as a dietary intervention for metabolic disorders caused by excessive fructose consumption.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"33 4","pages":"391-404"},"PeriodicalIF":3.3,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Economic evaluation of AI-assisted technologies in healthcare: A systematic review.","authors":"Wen-Tung Wu, Yi-Wen Chao, Tsung-Kun Lin, Ching-Kuo Huang, Ping-Hsuan Hsieh","doi":"10.38212/2224-6614.3570","DOIUrl":"10.38212/2224-6614.3570","url":null,"abstract":"<p><p>Artificial intelligence (AI) technologies are increasingly integrated into healthcare, yet their economic value remains uncertain. Traditional economic evaluation methods may not adequately capture the unique features of AI, including dynamic model evolution, scalability, and broader societal impacts. This systematic review synthesized existing evidence on the cost-effectiveness of AI-based healthcare interventions and assessed the methodological rigor of published studies. A comprehensive search identified health economic evaluations of AI applications published between September 2019 and March 2025, following PRISMA and SWiM guidelines and registered in PROSPERO (CRD42025641230). Eligible studies were full economic evaluations comparing AI-based interventions with non-AI alternatives, and data were extracted on study characteristics, analytical methods, decision-analytic models, perspectives, outcomes, and AI-specific costs. Methodological quality was evaluated using the CHEERS checklist. A total of 52 studies from 15 countries were included, most published after 2020, focusing on diabetic retinopathy screening, cancer detection, and cardiovascular disease applications. Cost-utility analysis was the predominant method (79%), followed by cost-effectiveness analysis (15%). Nearly all studies (98%) concluded that AI-based strategies were cost-effective, cost-beneficial, or cost-saving. However, reporting of AI-specific costs was inconsistent, while over 90% of studies detailed expenses such as software licensing, per-test charges, or maintenance fees, some omitted cost information entirely, limiting comparability. Overall, AI-based healthcare interventions are generally reported as cost-effective, but methodological heterogeneity, incomplete cost reporting, and potential publication bias constrain the reliability and comparability of current evidence. Standardized economic evaluation frameworks that incorporate comprehensive cost structures and account for the evolving nature of AI are urgently needed.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"33 4","pages":"487-500"},"PeriodicalIF":3.3,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795340/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carotenoids identified from slipper-shaped oyster Magallana bilineata powder residue exhibit potent biological activities.","authors":"Rhoda Mae C Simora, Raymund B Parcon, Andrea Roxanne J Anas, Tatsufumi Okino","doi":"10.38212/2224-6614.3564","DOIUrl":"10.38212/2224-6614.3564","url":null,"abstract":"<p><p>Carotenoids are a diverse class of biologically active compounds that contribute significantly to human health, serving vital functions in nutrition and overall well-being. Magallana bilineata, a commercially important oyster species, yields a shelf-stable powder residue possessing bioactivities with unknown specific compounds. Carotenoids are key marine bioactive compounds, but their presence in oysters remains underexplored. The present study aimed to identify the bioactive compounds from oyster powder residue through mass spectrometry for optimum utilization and value creation as a biomedical resource. The study employed solvent extraction of oyster powder residue, followed by fractionation using octadecylsilyl (ODS) column chromatography, liquid chromatography-mass spectrometry (LC-MS)-guided profiling, and ultra-high-performance liquid chromatography-ultra-high-performance liquid chromatography-elevated energy mass spectrometry-elevated energy mass spectrometry (UHPLC-MS^E) analysis to identify carotenoids, with bioactivity assays conducted to assess the cytotoxic, antimicrobial, antioxidant, and anti-inflammatory properties. The study obtained two HPLC fractions and enabled the identification of carotenoid compounds based on retention times and UHPLC-MS^E, with elemental compositions inferred from the observed mass-to-charge ratios. The bioactivities of the two HPLC fractions, identified as zeaxanthin in fraction 1 and a zeaxanthin/lutein isomeric mixture in fraction 2, were assessed. The zeaxanthin/lutein isomeric mixture exhibited higher effectiveness in MCF-7 cancer cell inhibition (IC₅₀ = 93.29 ± 0.07 μg/mL) than cisplatin, but both HPLC fractions showed strong antibacterial activity against Klebsiella pneumoniae and Escherichia coli. Moreover, notable antioxidant activity was observed in both fractions for 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity assays, while zeaxanthin demonstrated anti-inflammatory activity (43.68 ± 0.11%) comparable to aspirin (43.49 ± 0.17%). These findings suggest that the observed biological activities of the HPLC fractions may be the consequences of the adaptive response and filter-feeding behaviors of oysters, which result in the accumulation of bioactive carotenoids. This study offers a promising perspective on applying mass spectrometry techniques for advanced compound extraction and identification, and on utilizing oyster powder residue as a sustainable approach to waste valorization and as a functional ingredient for biomedical applications.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"33 4","pages":"434-450"},"PeriodicalIF":3.3,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12795335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145958348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted nanosponge-based treatment for pulmonary hypertension: A novel approach.","authors":"Jashanpreet Kaur, Gursimran Kaur, Chamanpreet Kaur, Sandeep Kumar","doi":"10.38212/2224-6614.3550","DOIUrl":"10.38212/2224-6614.3550","url":null,"abstract":"<p><p>Pulmonary Hypertension (PH) is a progressive and potentially fatal condition marked by high pulmonary artery pressure, resulting in heart failure and reduced oxygenation. Despite advancements in treatments, therapeutic options for PH remain limited, particularly in cases resistant to conventional therapies. In biomedical research, nanotechnology has become a potential area of study, presenting novel approaches to drug delivery and tissue targeting. Nanosponges, a class of nanoparticles with porous structures, have gained attention for their ability to encapsulate therapeutic agents, enhance drug stability, and provide controlled release. Nanosponges can be engineered to deliver vasodilators, anti-inflammatory drugs, and gene therapies directly to the pulmonary vasculature, minimizing systemic side effects and improving drug efficacy. Additionally, their unique surface properties allow for targeted delivery to specific cells or tissues involved in PH, such as the pulmonary arteries' smooth muscle and endothelial cells. This review explores the potential role of nanosponges in pulmonary hypertension, highlighting recent advances in their design and functionalization. The integration of nanosponges into PH therapy could revolutionize the treatment landscape, offering more effective and individualized treatment plans.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"33 3","pages":"213-223"},"PeriodicalIF":3.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510689/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precision nanomedicine for anxiety: challenges, opportunities, and future directions in targeted drug delivery.","authors":"Sehrabpreet Singh, Amanpreet Singh, Shruti Chittu, Shailesh Sharma","doi":"10.38212/2224-6614.3556","DOIUrl":"10.38212/2224-6614.3556","url":null,"abstract":"<p><p>Nanoparticle-based drug delivery system represents one of the challenging strategies suggested to improve anxiety disorder therapeutic approaches, clinic challenges of delayed action, side effect-free designing and poor patient compliance. Traditional pharmacological agents can increase drug bioavailability and target specific brain regions, whereas nanoparticle-mediated controlled release offers enhanced precision and sustained action. The others in this review concentrate on several kinds of nanoparticle, including lipid-based, polymeric-metallic, and responsive nanoparticles, their use in anxiety medication. In addition, emphasis is placed on precision medicine that pertain the delivery of treatment based on an individual's genetic, environmental and lifestyle aspects. Also, it is looked into how artificial intelligence is being integrated into personalized nanoparticle formulations. Toxicity, regulatory hurdles and scalability are briefly discussed and future directions on smart and biodegradable nanoparticles are underlined. The present review highlights advantages of nanoparticle treatments and outlines a future direction of precision nanomedicine for anxiety.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"33 3","pages":"241-251"},"PeriodicalIF":3.3,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510706/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145312071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of an analytical method for determination of bongkrekic acid in biofluids for toxin monitoring.","authors":"Ying-Tzu Shieh, Te-I Weng, Ju-Yu Chen, I-Ting Wang, Kevin Shu-Leung Lai, Chia-Mo Lin, Chu-Yun Teng, Guan-Yuan Chen","doi":"10.38212/2224-6614.3549","DOIUrl":"10.38212/2224-6614.3549","url":null,"abstract":"<p><p>Bongkrekic acid (BKA) poisoning is a severe foodborne illness with a high mortality rate. This study aimed to identify BKA in postmortem tissues from poisoning victims in Taiwan and to develop a reliable analytical method for detecting BKA in biofluids to aid clinical diagnosis and treatment. BKA was identified in postmortem samples using high-resolution mass spectrometry (HRMS) and confirmed with an independent ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) method. To meet clinical needs, a UHPLC-MS/MS method was developed and validated for BKA detection in plasma and urine. Method optimization included adjustments to ion source conditions for multiple reaction monitoring (MRM) transitions and avoidance of glass vials due to BKA adsorption onto free silanol groups. Chromatographic separation was achieved using a 50-mm Hypersil Gold C18 column within a 6-min run time. The validated UHPLC-MS/MS method successfully detected BKA in biofluids, enabling its application in identifying victims of foodborne poisoning. The method demonstrated high accuracy and efficiency, facilitating timely diagnosis and aiding in treatment strategies for critically ill patients. The developed UHPLC-MS/MS method provides a reliable approach for detecting BKA in clinical and forensic settings. Its implementation enhances diagnostic capabilities, improves patient outcomes, and supports monitoring of toxin elimination in cases of BKA poisoning.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"33 3","pages":"339-347"},"PeriodicalIF":3.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510690/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The rhythm of decline: Circadian disruption in neurodegeneration.","authors":"Jeewanjot Singh, Devinder Kumar, Jasleen Kaur, Amanpreet Singh","doi":"10.38212/2224-6614.3553","DOIUrl":"10.38212/2224-6614.3553","url":null,"abstract":"<p><p>Alzheimer's disease (AD) is a progressive neurodegenerative disorder with a multifactorial etiology involving genetic, environmental, and metabolic factors. Among these, circadian rhythm disruption has emerged as a crucial but under-explored contributor to disease progression. The circadian system, regulated by the suprachiasmatic nucleus (SCN), controls essential physiological functions such as the sleep-wake cycle, metabolism, and neuroendocrine signaling. Disruption of this system has been increasingly linked to key pathological features of AD, including amyloid-beta accumulation, tau hyperphosphorylation, and neuroinflammation. This review critically examines the mechanistic role of circadian misalignment in AD by analyzing studies on sleep disturbances, SCN degeneration, metabolic dysregulation, clock gene polymorphisms (BMAL1, CLOCK, PER, CRY), and gut-brain axis interactions. Evidence indicates that circadian abnormalities manifest as reduced melatonin secretion, impaired glymphatic clearance, and altered SCN signaling, all of which contribute to neuronal dysfunction and cognitive decline. Additionally, sleep deprivation has been shown to exacerbate amyloid-beta accumulation, while tau pathology can further disrupt circadian control, creating a vicious cycle. Dysregulated gut microbiota rhythms and associated metabolic changes further enhance neuroinflammatory responses, increasing AD risk. Diagnostic advances such as actigraphy, melatonin assays, and plasma biomarkers provide non-invasive methods for early detection of circadian misalignment. Therapeutic strategies targeting the circadian system-including light therapy, melatonin supplementation, and gene-based interventions-show promise in restoring circadian homeostasis and improving cognitive outcomes. Understanding and addressing circadian disruptions may offer novel and personalized approaches for delaying or mitigating Alzheimer's disease progression, highlighting the need for further research in this direction.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"33 3","pages":"224-240"},"PeriodicalIF":3.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repurposing dihydroartemisinin as a novel anticancer agent against colorectal cancer stem cells.","authors":"Meng-Han Wu, Chieh-Ju Sung, Fan-Lu Kung, Jih-Hwa Guh, Yeu Su, Lih-Ching Hsu","doi":"10.38212/2224-6614.3552","DOIUrl":"10.38212/2224-6614.3552","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is one of the leading causes of cancer-related death globally and discovering novel therapeutic agents to treat the disease, and prevent cancer metastasis and recurrence is an urgent medical need. Cancer stem cells (CSCs) capable of self-renewal and differentiation are generally considered the cause of tumor metastasis, recurrence and chemoresistance. Hence, targeting CSCs may be a promising strategy for the treatment of cancer. GATA6, a zinc finger transcription factor, contributes to tumorigenesis in CRC and is related to cancer stemness. GATA6-overexpressing stable clones OE4 and OE6 derived from HCT116 cells were previously established and exhibited increased stemness properties. In this study, we found that OE4 and OE6 cells displayed CSC-like properties, including higher expression levels of stemness-related proteins, increased sphere forming capacity and resistance to 5-fluorouracil. OE4 and OE6 cells also showed increased glucose uptake capacity, another hallmark of CSCs. Therefore, these two cell clones were employed as a CSC-like cell model to search for potential colorectal CSC-targeting drugs. Among several compounds tested, dihydroartemisinin (DHA), an antimalarial drug, exerted better anticancer activity toward OE4 and OE6 relative to the empty vector-transfected HCT116 cells. DHA also inhibited sphere formation and impaired glucose metabolism. DHA induced G0/G1 arrest and apoptosis. Moreover, DHA also induced reactive oxygen species and mitochondrial membrane potential loss. Thus, DHA caused mitochondrial damage which was confirmed by Seahorse mitochondrial stress test. DHA also increased LC3B-II and PINK1 protein levels, indicative of autophagy/mitophagy. In conclusion, repurposing DHA may be a potential strategy against colorectal CSCs and further validation using in vivo models is warranted.</p>","PeriodicalId":358,"journal":{"name":"Journal of Food and Drug Analysis","volume":"33 3","pages":"277-291"},"PeriodicalIF":3.0,"publicationDate":"2025-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12510712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145256927","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}