Journal of Environmental Management最新文献

{"title":"Fate and ecological risk assessment of phthalates in wastewater treatment plants","authors":"Ke Zhao,&nbsp;Ximing Zhang,&nbsp;Yue Wang,&nbsp;Xinrong Du,&nbsp;Su Wang,&nbsp;Mengnan Shen","doi":"10.1016/j.jenvman.2025.125778","DOIUrl":"10.1016/j.jenvman.2025.125778","url":null,"abstract":"<div><div>With the rapid increase in plastics use worldwide, phthalates (PAEs) as an endocrine disruptor, have been receiving attention. It is necessary to investigate the pivotal considerations affecting the degradation of PAEs and the ecological risk assessment in wastewater treatment plants. The presence and spatial distribution patterns of 16 PAEs across three wastewater treatment plants (WWTPs) in northern China were studied by collecting wastewater samples, and by comparing the mechanism of action of AAO and oxidation ditch process on the removal efficiency of long and short chain PAEs, we fill the gap in the study of comprehensive assessment and process-specific removal effect of multiple PAEs. The influent concentration of PAEs and the treatment process were the pivotal considerations in the primary and secondary treatments, respectively. In the primary treatment stage, long-chain PAEs were more readily removed compared to short-chain PAEs. Conversely, during the secondary treatment, short-chain PAEs were removed more effectively than long-chain PAEs, independent of the specific treatment process. Long-chain PAEs were more easily removed during the AAO processes. Furthermore, the ecological risk assessment of the effluent was conducted at each stage of the three WWTPs. The ecological risk assessment results of the three WWTPs for fish and Daphnia showed no risk, while that for green algae showed a low risk. These findings can be used as a model for controlling PAEs and provide important information on the occurrence and distribution of PAEs in WWTP and their ecological risks of effluents.</div></div>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"387 ","pages":"Article 125778"},"PeriodicalIF":8.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Electrochemical improvement of methane production via surface engineering of graphitic cathodes in anaerobic sequential batch reactors","authors":"Smruti Ranjan Dash ,&nbsp;Hyungmin Choi ,&nbsp;Jae Keun Song ,&nbsp;Dayoung Ko ,&nbsp;Changsoo Lee ,&nbsp;Jeonghwan Kim","doi":"10.1016/j.jenvman.2025.125826","DOIUrl":"10.1016/j.jenvman.2025.125826","url":null,"abstract":"<div><div>Five anaerobic sequential batch reactors (SBR), SBR 1−SBR 5 run in parallel were examined for biogas output trends under varying hydraulic retention times (HRT). SBR 1 was run without biomass for 1 month to study electrode stability and the effect of applied potential on sodium dodecyl sulfate (SDS) degradation. Polyaniline (PANI/Graphite) modification in reactors SBR 4 and iron-coated PANI (Fe-PANI/Graphite) in SBR 5 increased biogas production by almost 2.5 times compared to SBR 2 without electrodes. SBR 3 equipped with unmodified graphite rods was used as a control for cathode modifications. By decreasing HRT, cumulative methane production increased to 280 and 320 mL at 72 h and 350 and 500 mL at 48 h. Compared to SBR 2, an electric field increased daily biogas production. Methane composition in SBR 5 increased from 44% at 96-h to 71% at 48-h HRT after 30 days. SBR 4 recovered within 7 days after HRT modifications reduced methane output. The methane yield increased significantly with electric current in SBR 3 (2.6 times), SBR 4 (5.4 times), and SBR 5 (7.4 times). The effluent total organic carbon was stabilized at 15 mg/L for SBR 2 and SBR 3 and improved to below 5 mg/L for SBR 4 and SBR 5 during reactor operation. SBR 5, equipped with an Fe-PANI/Graphite cathode showing the lowest charge transfer resistance, developed distinct microbial community structures in both anodic and cathodic biofilms, compared to the other electrically assisted SBRs.</div></div>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"387 ","pages":"Article 125826"},"PeriodicalIF":8.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reclamation of available phosphorus and separation of heavy metals from sewage sludge via FeCl3-assisted electrokinetic treatment and pyrolysis","authors":"Xutong Wang ,&nbsp;Huwei Li ,&nbsp;Junxia Wang ,&nbsp;Wolfram Buss ,&nbsp;Anna Bogush ,&nbsp;Ondřej Mašek ,&nbsp;Youjun Zhang ,&nbsp;Fan Yu ,&nbsp;Beibei Yan ,&nbsp;Zhanjun Cheng ,&nbsp;Xiaoqiang Cui ,&nbsp;Guanyi Chen ,&nbsp;Konstantin Ignatyev","doi":"10.1016/j.jenvman.2025.125882","DOIUrl":"10.1016/j.jenvman.2025.125882","url":null,"abstract":"<div><div>Recycling of sewage sludge and the endogenous phosphorus (P) is a promising strategy for sustainable development, while the disposal of heavy metals (HMs) in sewage sludge and the recovery of targeted P species remain challenges. An innovative method coupling electrokinetic treatment with pyrolysis was proposed in the present study to achieve the effective reclamation of available P and the separation of HMs from sewage sludge. The pristine and FeCl₃-assisted electrokinetic treatment were employed for the removal of HMs from sewage sludge and to modify the P species, and the subsequent pyrolysis (300–700 °C) was conducted for the recovery of available P along with the production of biochar. The X-ray absorption near-edge spectroscopy (XANES), ³¹P liquid nuclear magnetic resonance (NMR) spectroscopy, and sequential chemical extraction were used to systematically determine the evolution of P during the combined treatment of sewage sludge. 19.69–24.80 % of Ni, Cu, and Zn were removed from sewage sludge after pristine electrokinetic treatment, and the HM removal efficiency was further elevated to 47.01–56.86 % with the assistance of FeCl₃. Consequently, in comparison with the raw sewage sludge-derived biochars (SBs), the biochars derived from FeCl₃-assisted electrokinetic treated sewage sludge (FESBs) contained much lower HM contents and showed higher stability of HMs. The FeCl₃-assisted electrokinetic treatment converted alkaline biochars dominated by poorly soluble Ca-phosphates into neutral to slightly acidic biochars dominated by Al/Fe-associated phosphates. This transformation greatly improved the available P concentrations determined by diffusive gradients in thin film in FESBs by 0.6–1.3 folds compared to untreated SBs. Therefore, coupling FeCl₃–assisted electrokinetic treatment with pyrolysis could be a promising strategy to achieve the reclamation of available P and the separation of HMs from sewage sludge.</div></div>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"387 ","pages":"Article 125882"},"PeriodicalIF":8.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of CO2 emission and energy consumption in CCUS for steel-chemical joint production","authors":"Huapeng Yang ,&nbsp;Chao Feng ,&nbsp;Tao Lin ,&nbsp;Rong Zhu ,&nbsp;Kai Dong ,&nbsp;Guangsheng Wei ,&nbsp;Mingwei Tu ,&nbsp;Fuhai Liu ,&nbsp;Ziheng Zhao ,&nbsp;Xin Ren ,&nbsp;Chunyang Wang","doi":"10.1016/j.jenvman.2025.125934","DOIUrl":"10.1016/j.jenvman.2025.125934","url":null,"abstract":"<div><div>Iron, steel, and coal chemical industries are typically large-scale CO₂-emitting industries. Therefore, identifying a suitable steel-chemical joint production (SCJP) process is crucial for reducing joint carbon emissions. This study examines China's first basic oxygen furnace gas (BOFG), CO purification (CP), CO₂ capture (CC), and CO₂ utilization for long-process smelting as case studies to compare and analyze the differences between conventional long-process smelting and the SCJP approach in terms of CO₂ emissions, energy consumption, and economic benefits. By applying the life cycle assessment (LCA) theory and the gray-box model, CO₂ utilization in blast furnaces (BF) and basic oxygen furnaces (BOF) was determined to be conducive to the reduction of carbon emissions during the entire process. However, the SCJP process increases its energy consumption due to the addition of CP and CC processes. Compared to the conventional long-process smelting, using the SCJP process, the reduction of CO₂ emission per ton of crude steel is 25.4 kg, the CO₂ fixed through a product CO and CO₂-based product is 76.2 kg, the energy consumption is increased by 9.78 kg of coal equivalent, and the final smelting cost per ton of steel is lowered by 12.30 RMB.</div></div>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"387 ","pages":"Article 125934"},"PeriodicalIF":8.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of acetochlor by microbial consortium AT1: microcosm centric microbiomic-metabolomics mechanisms and environmental remediation feasibility","authors":"Yumeng Dai ,&nbsp;Xinyu Guan ,&nbsp;Zhiyang Han ,&nbsp;Xu Li ,&nbsp;Xiujuan Wang ,&nbsp;Zhencheng Su ,&nbsp;Huiwen Zhang ,&nbsp;Xiang Li ,&nbsp;Mingkai Xu","doi":"10.1016/j.jenvman.2025.125892","DOIUrl":"10.1016/j.jenvman.2025.125892","url":null,"abstract":"<div><div>The excessive use of herbicide acetochlor (ACT) threatens crop health and the environment, necessitating effective remediation strategies. This study focused on a consortium named AT1, enriched from ACT-contaminated soil. Under optimized conditions (25 °C, pH 7, 1 % inoculum), AT1 almost completely degraded ACT (50–1000 mg/L) within 6–12 days. High-throughput sequencing of 16S rRNA gene revealed a reduction in community diversity over time, with <em>Sphingomonas</em> (58.6 %) and <em>Diaphorobacter</em> (26.43 %) as dominant taxa. A structure model and network analysis indicated strong microbial competition during the peak degradation. Predicted functions and liquid chromatography-mass spectrometry based metabolomics data identified benzene ring intermediates during ACT degradation, including 2,6-dimethylaniline, resorcinol, phenol, 3-ethyl-1,2-benzenediol, 1,2,3-trihydroxybenzene, phloroglucinol, and benzene-1,2,4-triol. Joint omics analysis revealed that AT1 likely degrades ACT via <em>N</em>-dealkylation by <em>Pseudomonas</em>, amide bond hydrolysis by <em>Diaphorobacter</em>, and carboxylation and hydroxylation by <em>Sphingomonas</em>, leading to the formation of these intermediate metabolites. Moreover, AT1 efficiently degraded key intermediates, particularly 2,6-dimethylaniline, phenol, and resorcinol, further enhancing ACT mineralization. Notably, AT1 efficiently degraded ACT in soil, resulting in a significant decrease in ACT environmental residues. These findings provide valuable insights for the discovery and identification of herbicide-degrading bacterial resources and the metabolic transformation of herbicides, and developing approaches for pollution control and biodegradation of amide herbicides.</div></div>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"387 ","pages":"Article 125892"},"PeriodicalIF":8.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrological characteristics strongly dominate the spatiotemporal variation of bacterioplankton sub-communities in the Yangtze River Estuary","authors":"Wanli Hou ,&nbsp;Jianghua Yu ,&nbsp;Xi Chen ,&nbsp;Shuang Chen ,&nbsp;Haobo Wu ,&nbsp;Yiqing Chen ,&nbsp;Jie Bai","doi":"10.1016/j.jenvman.2025.125944","DOIUrl":"10.1016/j.jenvman.2025.125944","url":null,"abstract":"<div><div>Seasonal variations in river discharge in estuarine ecosystems drive dynamic interactions between freshwater and seawater, significantly impacting the structure and functionality of bacterioplankton sub-communities. Seasonal saltwater intrusion during the dry season intensifies the dynamic variations in water quality and salinity between the northern and southern branches of the Yangtze River Estuary, further complicating the ecological processes governing bacterioplankton communities. Nevertheless, the mechanisms underlying these processes and their effects on distinct bacterioplankton sub-communities remain insufficiently explored. In this study, the composition, functions, and assembly mechanisms of bacterioplankton communities in the Yangtze River Estuary during different hydrological periods were examined using molecular technique and various statistical analysis methods. The results showed that rare sub-communities exhibited the highest α-diversity, abundant and transient sub-communities primarily contributed to the diversity differences across hydrological periods. Saltwater intrusion into the northern branch altered bacterioplankton community and weakened the distance-decay pattern of the transient and rare sub-communities. Additionally, stochastic processes governed the assembly of rare sub-communities, and saltwater intrusion disrupted their migration and dispersal patterns. In contrast, abundant and transient sub-communities maintain their stability by adjusting their ecological strategies in response to salinity changes. Functional analysis indicated that external nutrient inputs and hydrodynamic changes in the wet season promoted community functional diversity and activity, while abundant sub-communities in the dry season were more effective at occupying resources and performing specific functions related to carbon cycling. These findings highlight the relationships between bacterioplankton sub-communities and environmental changes in estuarine ecosystems, underscoring their key roles in biogeochemical cycling.</div></div>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"387 ","pages":"Article 125944"},"PeriodicalIF":8.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A low carbon multi-modal logistics network optimization: A novel neutrosophic mixed integer linear programming approach","authors":"Anurag Kumar,&nbsp;Shraddha Mishra","doi":"10.1016/j.jenvman.2025.125924","DOIUrl":"10.1016/j.jenvman.2025.125924","url":null,"abstract":"<div><div>This paper proposes a novel neutrosophic mixed integer linear programming (NMILP) model for designing a multi-period, multi-country, and multi-modal network in an uncertain environment. The uncertainty related to demand, production cost, transportation cost, carbon emission cost, capacity and delivery time is handled with triangular neutrosophic numbers. The proposed NMILP provides joint decision-making on several issues, including facility location, production allocation, the number of carrier trips required and transportation mode selection. The NMILP model aims to balance carbon emissions, delivery delays and overall network cost. We proposed a novel approach focusing on the concept of <span><math><mrow><mi>α</mi><mo>,</mo><mi>δ</mi><mo>,</mo><mtext>and</mtext><mspace></mspace><mi>γ</mi></mrow></math></span> cuts (variation parameters for truth, indeterminacy, and falsity membership functions). This transformation changes the initial NMILP into a comparable interval mixed-integer linear programming model. This method allows for meaningful analysis and interpretation of results, providing best-case and worst-case optimal solutions. A key advantage of this approach lies in its flexibility, enabling decision-makers to experiment and adjust the required acceptance, indeterminacy, and falsity levels while analysing results. The proposed NMILP is validated using a representative case of a reasonable size.</div></div>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"387 ","pages":"Article 125924"},"PeriodicalIF":8.0,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144124111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental quality and financial flows in emerging economies: Evidence from BRICS countries","authors":"Noor Hashim Khan ,&nbsp;Weijun He ,&nbsp;An Min","doi":"10.1016/j.jenvman.2025.125854","DOIUrl":"10.1016/j.jenvman.2025.125854","url":null,"abstract":"<div><div>This study investigates the relationship between environmental sustainability, financial flows, and green finance in BRICS nations over the period 1990–2022. Motivated by the urgent need to balance economic growth with ecological preservation in emerging economies, we employ advanced panel data techniques, including panel ARDL, cross-sectional dependence tests, CIPS unit root tests, and Westerlund cointegration analysis. Using comprehensive indicators such as the ecological footprint and its sub-components, our findings confirm the Environmental Kuznets Curve hypothesis, revealing a non-linear relationship between economic growth and environmental quality. Specifically, while GDP growth initially increases environmental pressures (coefficient: 24.028), higher income levels eventually lead to improvements (coefficient: −0.913). Green finance consistently mitigates environmental degradation (coefficients: −0.054 to −0.618), underscoring its critical role in sustainable development. Remittances exhibit mixed effects, positively influencing the overall ecological footprint (0.957) but negatively affecting built-up land (−0.426). These results provide actionable insights for policymakers, highlighting the importance of integrating green finance initiatives to moderate the environmental impacts of economic growth in emerging economies.</div></div>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"387 ","pages":"Article 125854"},"PeriodicalIF":8.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutralization of Acid Mine Drainage using thermally treated chromite mining waste","authors":"Maria Giannakoudi ,&nbsp;Evgenios Kokkinos ,&nbsp;Evangelos Tzamos ,&nbsp;Anastasios Zouboulis","doi":"10.1016/j.jenvman.2025.125936","DOIUrl":"10.1016/j.jenvman.2025.125936","url":null,"abstract":"<div><div>In this study, two different mining waste streams were combined in the context of circular and green economy. Acid mine drainage (AMD) is considered to be the main environmental issue of the corresponding industry due to its acidic nature and high concentration of heavy metals. On the other hand, a large volume of tailings is produced during chromite mining and beneficiation processes. These tailings are mainly ultrabasic rocks, namely, serpentine and olivine. Despite not posing a significant environmental concern, they need to be properly managed. This work aimed to evaluate the AMD treatment (neutralization and metal removal) by the chromite mining tailings, contributing to sustainable practices within the mining industry. As experimental material, field samples of ultrabasic rocks were obtained, sieved, homogenized, and thermally upgraded (700^oC). Artificial AMD was synthesized to simulate real conditions, and the homogenized material was tested under batch and column setups to determine its neutralization efficiency. According to the results, the AMD was successfully neutralized by the upgraded ultrabasic rock. In detail, an equilibrium pH value of 7.9 was achieved by applying a liquid-to-solid ratio of 12.5 after 1 h of reaction time. Regarding the continuous flow column setup, neutralization was obtained by applying an empty bed contact time equal to 30 min. The heavy metal removal efficiency was mainly dependent on their solubility and experimental duration. Even if most of them presented high removal rates, magnesium concentration increased since it was leached from the upgraded ultrabasic rock to the aquatic phase.</div></div>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"387 ","pages":"Article 125936"},"PeriodicalIF":8.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144115972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Context-dependent anaerobic oxidation of methane: Insight for methane emission mitigation","authors":"Wenwen Huang,&nbsp;Yi Tang,&nbsp;Mengchen Lu,&nbsp;Zhenzhen Peng,&nbsp;Shubing Li,&nbsp;Xunsen Chen,&nbsp;Fangrong Wei,&nbsp;Teng Guo,&nbsp;JinShao Ye,&nbsp;Yan Long","doi":"10.1016/j.jenvman.2025.125896","DOIUrl":"10.1016/j.jenvman.2025.125896","url":null,"abstract":"<div><div>Anaerobic oxidation of methane (AOM) exhibits context-dependent metabolic versatility, governed by electron acceptor heterogeneity and anthropogenic perturbations. This study investigates the AOM potential by simulating three environments, high-dissolved organic carbon (DOC), high-nitrate with moderate sulfate, and sulfate-enhanced conditions, to investigate AOM potential under controlled perturbations. Substrate conversion dynamics were observed by monitoring the variation of methane, sulfate, nitrate, iron, etc., and microbial community shifts were analyzed by 16S rDNA high-throughput sequencing. In the high-carbon condition, characterized by high DOC (5.65–21.83 mmolC·L⁻¹) but low nitrate and sulfate (both &lt;1 mmol L⁻¹) levels, methanogens such as <em>Methanobacterium</em> sp. IM1 and <em>Thermoplasmata</em> dominated the stage, overpowering anaerobic methanotrophic archaea (ANME), while no methane oxidation but obvious methane production occurred. Shifting to the scenario with high nitrate (3.51 mmolN·L⁻¹) and moderate sulfate (1.36 mmolS·L⁻¹), ammonium accumulation played the role of a spoiler. It weakened AOM process (K_mo = 0.58 d⁻¹) and stirred up a competitive relationship between sulfur-driven ammonium-oxidizing archaea (e.g., <em>Nitrososphaeraceae</em>, <em>Nitrosotaleaceae</em>) and methanotrophs (e.g., Marine group II, <em>Wosearchaeales</em>, <em>Roseiarcus</em>). However, once nitrate was consumed to a low level, sulfate reduction relieved the ammonium pressure and re-activated iron, the suppression of AOM eased (K_mo = 1.44 d⁻¹). Under the sulfate-enhanced circumstances, where the sulfate level increased to 1.47–2.55 mmolS·L⁻¹, the AOM process accelerated (K_mo = 4.02 d⁻¹) even under high-nitrate conditions (1.66 ± 0.12 mmolN·L⁻¹). Methanotrophs and sulfur-metabolizing bacteria then co-thrived, showing a close display of cooperation. Our findings offer a pivotal framework to clarify AOM's contribution to natural methane emissions and give a new perspective for the development of methane mitigation technologies.</div></div>","PeriodicalId":356,"journal":{"name":"Journal of Environmental Management","volume":"387 ","pages":"Article 125896"},"PeriodicalIF":8.0,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144123229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}