Journal of Basic and Clinical Reproductive Sciences最新文献

{"title":"Pre-implementation Genetics for Aneuploidy Interpretation in PGT-A Goals Indications and Improve Infertility Outcomes, ART, and How to Perform PGT-A","authors":"Aya Al-ibraheemi, Mustafa Zakaria, M. Ennaji, Senhaji R. Wassym, N. Louanjli, M. Zarqaoui, Ritu S. Santwani, Nisrine En-Naciri, Hafida Tarik, Romaissa Boutiche","doi":"10.31080/ASWH.2020.03.0171","DOIUrl":"https://doi.org/10.31080/ASWH.2020.03.0171","url":null,"abstract":"Preimplantation genetic testing for aneuploidy PGT-A has been widely applied today in assisted reproductive technology treatments centers over the world to select the health embryos for transfer with euploid cells and to enhance clinical outcomes such as embryo implantation, clinical pregnancy, and live birth rates. The PGT-A can be performed in during an IVF cycle, after fertilization where a biopsy can be taken from the blastocysts to be genetically tested. PGT-A indication can be to decrease recurrent miscarriage, increase the pregnancy rate in advance maternal age, and increase single embryo transfer. This review also demonstrates new PGT-A method which is called non-invasive PGT-A, to prevent the embryo development distracting and removing extra cells from the embryo, through analyzing leaked DNA.","PeriodicalId":356195,"journal":{"name":"Journal of Basic and Clinical Reproductive Sciences","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121761036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Follicular Fluid Aspiration and Oocyte Retrieval Techniques, Several Flushing, Difficulty Situations Damage Risk to the Cumulus Complex and Complications","authors":"M. Zarqaoui, Mustafa Zakaria, N. Louanjli, Nisrine En-Naciri, M. Ennaji, M. Jamil, A. Kabit, Saadani Brahim, Boutiche Romaissa","doi":"10.31080/ASWH.2020.02.0146","DOIUrl":"https://doi.org/10.31080/ASWH.2020.02.0146","url":null,"abstract":"In Assisted Reproductive Technology (ART), oocyte retrieval is a vital step. It was initially performed through laparoscopy, which was complex and difficult and of low efficiency. Ultrasound-guided transvaginal oocyte retrieval was safer and more effective; it is presently the standard operation for in vitro fertilization (IVF) treatment. Theoretically, oocyte retention is achievable after the initial aspirate due to abnormal development of the follicle or oocyte and human technical factors, and such retention could be overcome by recurrent follicular flushing. Follicular flushing is considered to maximize the number of oocytes retrieved and thereafter to improve the rate of IVF pregnancy. There are a number of factors that can affect egg collection and/or egg damage. These involve variables like pump vacuum flow, velocity, needle bore size and length, follicle pressure and size, and collection techniques. Cook Medical Technology, Brisbane, has developed appropriate equipment to study the factors influencing the success of egg collection and the cause of egg trauma.","PeriodicalId":356195,"journal":{"name":"Journal of Basic and Clinical Reproductive Sciences","volume":"166 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132043051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on the incidence, interaction, and future perspective on Zika virus","authors":"L. Afolabi, Musbahu M Sani, W. Okunowo","doi":"10.4103/2278-960X.194469","DOIUrl":"https://doi.org/10.4103/2278-960X.194469","url":null,"abstract":"Zika virus (ZIKV) belongs to the family Flaviviridae and genus Flavivirus. It is a single-stranded positive-sense ribonucleic acid (RNA) virus, has its origin traced to Zika forest in Uganda. Its infection leads to ZIKV fever, characterized by arthralgia, myalgia, rash, conjunctivitis, and asthenia. Clinical presentation of the infection is nonspecific and may often be confused with symptoms of other flaviviral diseases (dengue, West Nile [WN], and chikungunya). Recently, ZIKV has been associated with congenital malformations and neurological complications such as microcephaly and Guillain-Barre′ syndrome. The viral tropism revealed an infection of the skin fibroblasts, keratinocytes, and immature dendritic cells through enhanced expression of dendritic cell-specific intracellular adhesion molecule 3-grabbing nonintegrin or anexelecto (Greekword: ′uncontrolled′) and tyrosine protein kinase receptor 3 systems. Silencing of T-cell immunoglobulin (Ig) and mucin domain 1 (TIM-1) and AXL RNAs has shown blockage of viral entry through their anti-TIM-1 and anti-AXL antibodies, hence serving as a potential target for ZIKV drug development. Biotechnological approaches targeted toward ZIKV vector control include the development of transgenic mosquitoes to disrupt the genome pool of wild strains and use of an endosymbiotic bacterium to prevent replication of arboviruses within its vector. Other approaches include the use of gene drive and exploration of the genetic redundancy to disrupt the receptors used by the virus to gain entry into its host. It is also imperative to explore the modality through which neutralizing antibodies block this viral infection as this may prove as a potential target to arrest the viral life cycle.","PeriodicalId":356195,"journal":{"name":"Journal of Basic and Clinical Reproductive Sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125378587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contraceptive-seeking behavior of women attending antenatal care in a developing country: A veritable tool for slowing population growth","authors":"E. Iwuoha, E. Oparaocha, H. Nwokeukwu","doi":"10.4103/2278-960X.194478","DOIUrl":"https://doi.org/10.4103/2278-960X.194478","url":null,"abstract":"Background: The use of modern contraceptives has been embraced by developed nations as a means of achieving controlled growth rate. Nigeria, Africa′s most populous nation at 158 million with a growth rate of 2% is expected to grow to 730 million by the end of this century. There is need for regular assessment of the knowledge and practice of contraception among women of childbearing age. Aim: The aim of this study is to determine the contraceptive-seeking pattern among women attending antenatal care in an urban city in a developing country and the factors affecting usage. Subjects and Methods: This was a cross-sectional study of 430 antenatal women using structured self-administered questionnaires on randomly selected attendees who consented to participate. Results were analyzed using SPSS Version 16 (Chicago, IL, USA). Results: Although majority of the women 71.2% (306/430) wished to have between 1 and 4 children, only 20% (87/430) had on their own sought for advice on contraception from a health facility. The percentage of women who had a knowledge of contraception was 61.4% (264/430) with highest source of information from antenatal clinics. Among the women, 41.2% (177/430) have used contraception since getting married; 16.1% (69/430) used natural and withdrawal methods while only 25.1% (108/430) used modern contraceptives. Reasons for nonusage of some modern contraceptives given by 340 women include fear of side effect 53.2% (181/340), objection from partner 7.9% (27/340), conflict with religious beliefs 4.1% (14/340) while 34.4% (117/340) had no reason for not using contraceptive. Conclusion: Contraceptive-seeking is low. Programs aimed at encouraging women to deliver in health facilities should be intensified. Health workers should utilize every opportunity to educate on contraceptive choices.","PeriodicalId":356195,"journal":{"name":"Journal of Basic and Clinical Reproductive Sciences","volume":"154 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121396549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Titrated misoprostol versus dinoprostone for labor induction","authors":"A. Yehia, Ihab Abd El Fattah, Karam M. Bayoumy, Ibrahim Anwar Abdelazim, Y. Elshehawy, N. Rabei, S. Daoud, A. Essam","doi":"10.4103/2278-960X.194473","DOIUrl":"https://doi.org/10.4103/2278-960X.194473","url":null,"abstract":"Background: Misoprostol is as effective as dinoprostone for labor induction with low cost and temperature stability. Aim: This study designed to compare titrated misoprostol regarding its safety and efficacy with dinoprostone for induction of labor. Subjects and Methods: Women with a single pregnancy, above 37 weeks′ gestation, cephalic presentation, modified Bishop′s score <8, and not in labor with reassuring fetal heart rate, admitted for labor induction enrolled in this randomized controlled study. Studied women were randomized into; Group I: received oral misoprostol titrated in sterile water (200 μg tablet was dissolved in 200 ml sterile water [1 μg/ml]), starting dose of 20 μg misoprostol required, given every 2 h, and stopped if adequate contractions obtained and Group II: received vaginal dinoprostone tablet maximum two doses followed by augmentation of labor by oxytocin ± amniotomy if there is no uterine contractions after two doses of dinoprostone. In Group I, if the contractions were inadequate after two doses of oral titrated misoprostol (20 μg [20 ml]), the starting dose increased to 40 μg (40 ml), escalating the dose from 5 to 10 ml (45-50 μg), and 20 ml (60 μg) maximum ± amniotomy. If the uterine contractions were adequate, the next dose of misoprostol or dinoprostone was omitted. Statistical analysis done using Student′s t-test for quantitative data and Chi-square test for qualitative data. Results: Induction-to-delivery time was significantly longer in misoprostol than dinoprostone group (975 vs. 670 min, respectively), (P = 0.01). About 20.2% (21/104) of women in misoprostol group did not deliver vaginally within 24 h compared to 7.4% (8/108) in dinoprostone group (significant difference, P = 0.01). Augmentation of labor was significantly high in dinoprostone (37.96% [41/108]) compared to misoprostol group (10.6% [11/104]) (P < 0.01). Conclusion: Titrated misoprostol for induction of labor seems to be associated with significantly longer induction-to-delivery time, low incidence of vaginal birth within 24 h, and less need for augmentation of labor compared to vaginal dinoprostone.","PeriodicalId":356195,"journal":{"name":"Journal of Basic and Clinical Reproductive Sciences","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133227482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inverse correlation between insulin-like growth factor-1 and leptin levels in preeclampsia","authors":"P. Panjeta, V. Ghalaut, J. Bala, S. Nanda, S. Kharb","doi":"10.4103/2278-960X.194480","DOIUrl":"https://doi.org/10.4103/2278-960X.194480","url":null,"abstract":"Background: Preeclampsia is the major cause of maternofetal and neonatal morbidity and mortality. Insulin-like growth factor (IGF) system has a crucial role in correct embryonic and placental development and growth. Conflicting data are available regarding IGF-1 in preeclamptic mothers. The extent to which leptin per se mediates the fetal growth and developmental abnormalities associated with preeclampsia remains to be clarified. Aim: Hence, the present study was planned to assess IGF-1 and leptin levels in maternal and cord blood of preeclamptics and to compare them with normotensive pregnant women. Subjects and Methods: The present study was conducted in the Department of Biochemistry in collaboration with the Department of Obstetrics and Gynaecology, Pt. B.D. Sharma, PGIMS, Rohtak. Totally, 25 normotensive pregnant and 25 preeclamptic women were enrolled, and serum leptin and IGF-1 levels were analyzed in maternal and cord blood of women by enzyme-linked immunosorbent assay. Results: IGF-I levels were lowered in maternal blood of preeclamptic as compared to normotensive mothers (P < 0.001). Leptin levels were significantly increased in preeclamptic mothers as compared to normotensive mothers (P < 0.001). Leptin had a positive correlation with IGF in both groups and it is highly statistically significant in preeclamptic mothers. Conclusion: Findings of the present study suggest that IGF-1 and leptin play a central role in controlling fetal growth.","PeriodicalId":356195,"journal":{"name":"Journal of Basic and Clinical Reproductive Sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125027087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of progesterone therapy versus diet modification on constipation during pregnancy","authors":"M. Farghali, Ibrahim Anwar Abdelazim, A. Awadalla, Amr Aziz Khalifa, Y. Elshehawy, A. Omu, Assem A. M. Elbiaa","doi":"10.4103/2278-960X.194477","DOIUrl":"https://doi.org/10.4103/2278-960X.194477","url":null,"abstract":"Background: Pregnant women may experience constipation for the first time or their existing constipation symptoms increase in severity during pregnancy. Aim: To compare the effect of progesterone versus diet modification in the treatment of constipation during pregnancy. Subjects and Methods: Women aged ≥18 years with functional constipation according to the Rome III criteria from obstetrics outpatients′ clinic and midwife practices included in this study. Participants divided into two groups; control group managed with diet modifications and study group pregnant women with threatened miscarriage and advised to take vaginal progesterone ≥1 week. Participants completed a nonvalidated questionnaire created by the authors during the whole week before intake of progesterone or diet modifications and after treatment phase. Independent Student′s t-test and Chi-square (χ2 ) test were used for statistical analysis to compare between two studied groups. Primary outcome measures; change in defecation frequency. Results: Sensation of anorectal obstruction and sensation of incomplete evacuation were significantly less in Group B (progesterone therapy) compared to Group A (diet modification) (54% [154/281] and 62.98% [177/281] vs. 89.76% [614/684] and 91.08% [623/684], respectively) (P = 0.04 and 0.03, respectively). Straining during defecation and manual maneuvers to facilitate evacuation were significantly less in Group B compared to Group A (63.7% [179/281] and 19.9% [56/281] vs. 94.59% [647/684] and 86.54% [592/684], respectively) (P < 0.01 and 0.02, respectively). Episodes of abdominal pain and presence of reflux episodes were also significantly less in Group B compared to Group A (18.5% [52/281] and 17.43% [49/281] vs. 84.11% [589/684] and 75% [513/684], respectively) (P = 0.01 and 0.03, respectively). Conclusions: Estrogen, rather than progesterone, may be a detrimental factor of constipation during pregnancy via decreased bowel movement. Progesterone therapy seems to be effective in the treatment of functional constipation during pregnancy. A randomized placebo controlled trial is required to confirm the data of this study.","PeriodicalId":356195,"journal":{"name":"Journal of Basic and Clinical Reproductive Sciences","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132472656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Filariasis in pregnancy: Prevalent yet less-known global health burden","authors":"G. Chandrakala, Momina Zulfeen","doi":"10.4103/2278-960X.194484","DOIUrl":"https://doi.org/10.4103/2278-960X.194484","url":null,"abstract":"Parasitic infections affect tens of millions of pregnant women worldwide. Lymphatic filariasis is a vector-borne disabling parasitic infection and is an endemic disease in many parts of Southeast Asia, especially South India, with most infections caused by Wuchereria bancrofti. The aim of this article is to present a rare case of incidental filariasis in pregnancy with unexpected outcome. We report a normotensive 45-year-old multigravida with 7 months gestation, moderate anemia, fever, inguinal lymphadenopathy, and elephantiasis. Her peripheral smears were positive for microfilaria while ultrasound revealed intrauterine fetal death and chronic abruption. She was negative on risk factors for abruption, except for advanced maternal age. This case warrants more global attention to the management of parasitic infection in pregnancy.","PeriodicalId":356195,"journal":{"name":"Journal of Basic and Clinical Reproductive Sciences","volume":"81 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122964351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of educational level on knowledge and practice of breast self-examination among women in Sokoto, Nigeria","authors":"A. Sani, F. Naab, Lydia Aziato","doi":"10.4103/2278-960X.194482","DOIUrl":"https://doi.org/10.4103/2278-960X.194482","url":null,"abstract":"Background: Breast cancer is the leading cause of cancer death among women worldwide. Breast self-examination (BSE) remains the most effective means of early detection of breast cancer among women in middle- and low-income countries where mammography is neither available nor affordable. Aim: This study explored the influence of educational level on knowledge and practice of BSE among women in Sokoto, Nigeria. Subjects and Methods: A quantitative cross-sectional design was employed using structured questionnaire. A sample of 400 participants was selected for the study using multistage sampling technique among educated women in Sokoto. Statistical Package for Social Sciences (SPSS version 20.0, IBM Corp, New York) was used for data analysis. Chi-square was used to establish significant relationships. Results: Findings showed that 75% (294/392) of women had average knowledge of BSE, but only 34.4% (135/392) practiced BSE regularly. Moreover, there was a significant relationship between educational level and the knowledge and practice of BSE. Conclusion: Findings of this study suggest that though women may have knowledge about BSE, the practice of BSE is sporadic.Therefore, there is a need for health care practitioners to provide women with BSE education and emphasize on the need for regular practice of BSE.","PeriodicalId":356195,"journal":{"name":"Journal of Basic and Clinical Reproductive Sciences","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125406725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microdeletion of Y-chromosome and their high impact on male infertility","authors":"A. Saxena, R. Gupta","doi":"10.4103/2278-960X.194466","DOIUrl":"https://doi.org/10.4103/2278-960X.194466","url":null,"abstract":"Male infertility is a multifactorial genetic disorder. WHO defined infertility as an inability to conceive naturally after at least 1‐year of unprotected intercourse. It is expected that 15% of couples worldwide who seeks children have infertility while male factor alone contributes about 50% in childless couples. In more than half of infertile male are unknown idiopathic causes. Semen analysis shows abnormal conditions such as azoospermia, oligozoospermia, teratozoospermia, asthenozoospermia, necrospermia, and pyospermia.[1‐6] The prevalence of primary and secondary infertility varies between 29% and 71%, but about 30% of cases of reduced infertility are still unknown. The Y‐chromosomes play a significant role in maintaining fertility in human. Hence, it is essential to understand the molecular structure of Y‐chromosome and their regions associated with infertility. Y‐Chromosome is one of the smallest chromosomes. It consists of euchromatic, heterochromatic regions, and covered 95% by male‐specific region. There are 60 million nucleotides including 156 transcription units, 78 protein‐coding genes, and 27 distinct proteins as shown in Figure 1.","PeriodicalId":356195,"journal":{"name":"Journal of Basic and Clinical Reproductive Sciences","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115001530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}