Turkiye parazitolojii dergisi最新文献

{"title":"Antimicrobial Peptides and Their Anti-<i>Leishmanial</i> Efficacies on <i>Leishmania</i> tropica Promastigotes <i>In vitro</i>.","authors":"Nihan Ünübol, İbrahim Çavuş, Tuba Polat, Özgür Kurt, Ahmet Özbilgin, Tanıl Kocagöz","doi":"10.4274/tpd.galenos.2024.48658","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2024.48658","url":null,"abstract":"<p><strong>Objective: </strong>Antimicrobial resistance is a real threat to humanity. Pentavalent antimonials are reported non-effective in leishmaniasis treatment today, in countries like India. New treatment options have been assessed worldwide lately. Antimicrobial peptides (AMP) are the leading antibiotic candidates due to their large spectrum, fast efficacy, and low resistance risks. Cathelicidins are the AMP with well-documented antimicrobial activities against bacteria, fungi, and protozoa, over their positively charged membranes. Here, we aim to design cathelicidine-like helical peptides (CLHP), and compare their anti-<i>Leishmanial</i> efficacies <i>in vitro</i>, with meglumine antimoniate (MA) on <i>Leishmania tropica</i>.</p><p><strong>Methods: </strong>A total of five study [TN-1-5] and two control (MA and non-drug) groups were formed. Cryopreserved <i>L. tropica</i> isolate was thawed and cultivated in Novy-MacNeal-Nicolle medium and then in RPMI. Five different CLHPs (TN1-5) were diluted in dimethyl sulphoxide. A total of 150 uL of CLHPs and MA were added into the first wells of the test plaques, followed by serial dilutions that revealed doses within 4 and 512 ug/mL. Then, 100 uL of cultures including 1x10⁸/mL of <i>L. tropica</i> promastigotes were added into each well. Viability of promastigotes was checked with XTT, while the parasite count was assessed at 24^th and 48^th hours.</p><p><strong>Results: </strong>TN3 was effective at 32 ug/mL. All tested CLHPs exhibited varying degrees of anti-<i>Leishmanial</i> activities, except TN5, even at its highest dose.</p><p><strong>Conclusion: </strong>TN3 showed a particular efficacy against <i>L. tropica</i> <i>in vitro</i>. Further studies including <i>in vivo</i> testing of the candidate's both efficacy and toxicity are essential.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 3","pages":"135-141"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Evaluation of Intestinal Protozoa Parasites in Patients Presenting to Kafkas University Health Research and Application Hospital Between 2019-2022.","authors":"Hilal Bedir, Neriman Mor, Ahmet Deniz, Mükremin Özkan Arslan","doi":"10.4274/tpd.galenos.2024.52386","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2024.52386","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to retrospectively evaluate the prevalence of protozoan parasites in stool samples collected from patients presenting with various gastrointestinal complaints to the Medical Parasitology Laboratory of Kafkas University Research and Application Hospital between 2019 and 2022.</p><p><strong>Methods: </strong>Stool samples were initially examined using the native-Lugol method for the detection of protozoan parasites, followed by the formol-ethyl acetate sedimentation method, Giemsa, and carbol fuchsin staining methods. Specific immunochromatographic card tests were used for the diagnosis of <i>Entamoeba histolytica, Cryptosporidium</i> spp., and <i>Giardia intestinalis</i>.</p><p><strong>Results: </strong>Of the 2.267 stool samples examined over the four-year period from January 2019 to December 2022, 7.63% were found to contain one or more protozoan parasites. Among these parasites, <i>Entamoeba histolytica</i> was detected at the highest rate of 4.06%. The other parasite species were identified as follows: <i>Blastocystis</i> spp. 1.15%, <i>Entamoeba</i> spp. and <i>Entamoeba coli</i> each 0.52%, <i>Giardia intestinalis</i> 0.48%, <i>Endolimax nana</i> 0.17%, and <i>Entamoeba histolytica/dispar</i> 0.08%.</p><p><strong>Conclusion: </strong>This study indicates that despite a decrease in the prevalence of intestinal protozoan infections in the Kars region, these infections remain a significant public health issue. Therefore, improvements in hygiene and sanitation conditions, increased public health education, and the widespread implementation of early diagnosis and treatment methods are necessary. Special measures should be taken to protect vulnerable groups, particularly children and the elderly.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 3","pages":"178-183"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mysterious Allergy Caused by Tick Bite: Alpha-Gal Syndrome.","authors":"Muhammed Nalçacı","doi":"10.4274/tpd.galenos.2024.97720","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2024.97720","url":null,"abstract":"<p><p>Alpha-Gal syndrome (AGS) manifests as an intricate allergic response characterised by the formation of specific immunoglobulin E (IgE) antibodies targeting a carbohydrate termed galactose-a-1.3-galactose (a-Gal). Alpha-Gal antigens, which play a role in AGS, have been detected in the salivary glands and saliva of various tick species, especially <i>Amblyomma americanum</i>. Identifying these antigens in tick saliva underlines the potential role of tick bites in sensitising individuals to a-Gal and contributes to the complex immunological processes associated with AGS. When people with a-Gal allergy eat beef, pork, lamb, or the flesh of other mammals, they experience an allergic reaction that causes various symptoms, including rash, nausea, vomiting, and diarrhoea. In some cases, AGS can be life-threatening requiring emergency medical attention. Moreover, these reactions do not occur only due to red meat; intake of medical drugs, vaccines, and antidotes containing a-Gal epitopes can also trigger allergies. The fact that the symptoms causing IgE antibodies are directed against a carbohydrate moiety the unusual delay between food consumption and the onset of symptoms, and the differences in the reactions shown by a-Gal allergy make a-Gal syndrome an unprecedented allergic disease and distinguish it from other food allergies. Interestingly, a-Gal antigens involved in the development of AGS have been discovered in salivary secretions of different tick species in several continents. However, the underlying causes of a-Gal-specific IgE production and immune responses to tick bites are not fully understood. This complex system is crucial for identifying and developing new therapies for the disease. This article reviews the evolution of a-Gal, the current understanding of AGS and its relationship to tick species.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 3","pages":"195-207"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comprehensive Bibliometric Analysis of Research Trends About Congenital Toxoplasmosis.","authors":"Mustafa Bağcı, Özlem Ulusan Bağcı","doi":"10.4274/tpd.galenos.2024.26056","DOIUrl":"10.4274/tpd.galenos.2024.26056","url":null,"abstract":"<p><strong>Objective: </strong><i>Toxoplasma gondii</i> is an obligate intracellular protozoon that infects approximately one-third of the human population. The parasite could transmit from mother to fetus in cases of acute infection during pregnancy and cause complications in the fetus. The bibliometric analysis is a popular research area that evaluates all the studies indexed in particular databases on a subject.</p><p><strong>Methods: </strong>This article puts forth bibliometric review of the literature on maternal and congenital toxoplasmosis research indexed in the Web of Science database between 1945 and 2024. VOS viewer, Web of Science and MS Office Excel 17 programs were used in the study.</p><p><strong>Results: </strong>The results of the search showed 1476 publications. The countries that most contributed to the literature were France (n=306, 20.73%), the USA (n=229, 15.52%), and Brazil (n=146, 9.89%). The most cited country was also France (n=10271, 35.52%), followed by the USA (n=9113, 31.51%), and England (n=2611, 9.03%). The top three countries by number of citations per document were Denmark (44.88), the USA (39.79) and France (33.57). The five departments with the most publications are Pediatrics (20.26%), General Internal Medicine (18.16%), Infectious Diseases (16.8%), Obstetrics (14.57%), and Immunology (11.86%). Wallon M. (n=57), Peyron F. (n=49), Thulliez P. (n=36) and Vilena I. (n=36) were the leading authors in terms of contribution to the literature. The five most published journals were Pediatric Infectious Disease Journal (3.66%), Journal of Clinical Microbiology (2.78%), Lancet (2.3%), Presse Medicale (1.76%), and American Journal of Obstetrics and Gynecology (1.63%).</p><p><strong>Conclusion: </strong>France is one of the countries that pays the most attention to congenital toxoplasmosis and compatible with this, in our study, the country with the highest number of studies on congenital toxoplasmosis was France. It is thought that drawing more attention to this issue and conducting more studies in countries where the disease is common might yield successful results, as in France.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 3","pages":"142-149"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Seropositivity of Anti-<i>Toxoplasma gondii</i> Antibodies and Possible Risk Factors in Pregnant Women with Diabetes at Risk.","authors":"Nazlı Aksoy Sanay, Neriman Mor, Dilek Şahin","doi":"10.4274/tpd.galenos.2024.27146","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2024.27146","url":null,"abstract":"<p><strong>Objective: </strong>Toxoplasmosis is a parasitic infection caused by <i>Toxoplasma gondii</i>. Immunocompromised individuals and pregnant women are at risk, with the latter group being susceptible to miscarriages. This study aimed to determine the seropositivity of <i>T. gondii</i> antibodies and potential risk factors in pregnant women diagnosed with diabetes mellitus.</p><p><strong>Methods: </strong>The research was conducted at the Ankara City Hospital Perinatology Clinic between October 2021 and June 2022. The study included 277 pregnant women diagnosed with diabetes mellitus and 277 healthy pregnant women who had given birth. Retrospective analysis of anti-<i>T. gondii</i> immunoglobulin (Ig)G and IgM levels was performed for patients between January 2020 and February 2022. Participants were administered an informed consent form and a questionnaire. Data were analysed using SPSS 22.</p><p><strong>Results: </strong>Among pregnant women with diabetes, IgG seropositivity was 18.4%, IgM was 0.0%, and IgG+IgM was 0.0%. In healthy pregnant women, IgG seropositivity was 12.3%, IgM was 0.4%, and IgG+IgM was 0.4%. Overall, seropositivity rates were 15.3% for IgG, 0.2% for IgM, and 0.2% for IgG+IgM. The difference between the two groups was statistically significant (p<0.05). Among pregnant women with diabetes, there was a significant statistical difference (p<0.05) in anti-<i>T. gondii</i> IgG seropositivity related to education, employment status, number of pregnancies and live births, history of toxoplasmosis diagnosis in children, previous toxoplasmosis diagnosis, hygiene, nutrition, and social habits. Among healthy pregnant women, significant statistical differences were found (p<0.05) in IgG seropositivity related to age, income, education level, number of pregnancies and live births, previous toxoplasmosis diagnosis, hygiene, nutrition, and social habits. No invasive interventions were performed on infants born to seropositive mothers, and perinatal data were not available.</p><p><strong>Conclusion: </strong>The seroprevalence of toxoplasmosis in Ankara appears to be decreasing, but <i>T. gondii</i> infections continue to pose a public health concern and are significant in pregnant women with diabetes mellitus.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 3","pages":"164-170"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Parasitic Infections and Host Tissue Response in Histopathology: A Rare Retrospective Research Study from Rural India.","authors":"Mani Krishna, Seema Dayal","doi":"10.4274/tpd.galenos.2024.04706","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2024.04706","url":null,"abstract":"<p><strong>Objective: </strong>Parasite are living organisms which survive on another living being for their nourishment and survival. When these parasites resides on human body, they bring about inflammatory response. This inflammatory response leads to tissue reaction. Tissue response on microscopy appear as an eosinophilia, abscess and granulomas. This study was planned with the objective to know the frequency of parasite infection, tissue response in parasite infection and its comparison in terms of variables like age, sex and the type of parasite.</p><p><strong>Methods: </strong>This is a retrospective study, conducted in the department of pathology. A total of 26 cases of parasitic infections in human specimens reported in our department from January 2008 to December 2019 were included in this study. On all archived cases hematoxylin and eosin and where ever required periodic acid schiff was applied. These slides were thoroughly examined and clinicopathological correlation was studied.</p><p><strong>Results: </strong>Age range of patients was 5 years to 70 years. Maximum number of patients were belonging to 11-20 year age group. Male to female ratio was 1:2. Among the 26 cases, there were 9 cases (34.62%) of hydatid cyst, six cases of <i>Entamoeba histolytica</i> (23.07%), four cases of <i>Enterobious vermicularis</i> (15.38%), and two cases (7.69%) each of <i>Ascaris lumbricoides</i>, filaria and cysticercosis respectively. A specific tissue response seen in cysticercosis having chronic inflammatory cells, palisaded epithelioid cells granuloma and giant cell reaction while other showed inflammatory cells infiltration.</p><p><strong>Conclusion: </strong>Clinically diagnosis of parasitic infection in each and every case is not possible, similarly radiological investigation is also suggestive only. Histopathology examination is the benchmark investigation to diagnose parasite infection and tissue reaction to the host. Histopathology examination must be implicated in every case to identify parasite and tissue reaction so that the patients can be managed accordingly before the complications rises.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 3","pages":"150-154"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381814","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Factors Associated with Gut Microbiota in <i>Demodex</i>-associated Skin Conditions.","authors":"Fatmagül Gülbaşaran, Seray Sarımustafa, Özlem Özbağçıvan, Şükran Köse, Emre Avcı","doi":"10.4274/tpd.galenos.2024.93064","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2024.93064","url":null,"abstract":"<p><strong>Objective: </strong>This study describes the relationships of factors related to gut microbiota and skin conditions associated with <i>Demodex</i>, including demodicosis, rosacea, and perioral dermatitis.</p><p><strong>Methods: </strong>A total of 113 patients from Dokuz Eylül University Hospital Dermatology Department answered a cross-sectional questionnaire. They consisted of 42 cases of <i>Demodex</i>-related skin diseases and 71 healthy controls. Demographic data and medical history, dietary and lifestyle habits, and gastrointestinal symptoms were recorded. Statistical analysis included descriptive statistics, chi-square tests, Fisher's Exact tests, independent samples t-tests, and logistic regression methods.</p><p><strong>Results: </strong>Our findings identified alcohol consumption [odds ratio (OR)=11.13, 95% confidence interval (CI): 4.11-17.22, p<0.01] and smoking (OR=10.32, 95% CI: 2.47-21.57, p<0.01) as strong risk factors for <i>Demodex</i>-related conditions. Low water intake (0-1 liter per day) (OR=3.39, 95% CI: 2.08-5.57, p=0.03) and infrequent exercise (less than 1 hour per week) (OR=4.87, 95% CI: 2.70-12.54, p=0.02) were also significant risk factors. Additional factors associated with increased <i>Demodex</i> risk included reduced bowel movements (OR=2.71, 95% CI: 1.45-4.06, p=0.01) and higher pet ownership (OR=2.85, 95% CI: 2.13-4.27, p=0.03). Although vegetarian and high-fat diets showed some associations, they were not independently significant.</p><p><strong>Conclusion: </strong>This study demonstrates key environmental and lifestyle factors, such as low water intake, infrequent exercise, reduced bowel movements, higher pet ownership, alcohol consumption, and smoking, that are significantly associated with <i>Demodex</i>-related skin conditions. These factors, related to gut microbiota, may provide valuable insights for managing these skin conditions and suggest promising directions for future research.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 3","pages":"171-177"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the Distribution of Intestinal Parasites Detected in the Parasitology Laboratory of Çukurova University Faculty of Medicine Between 2017 and 2021.","authors":"Mehtap Demirkazık, Eylem Akdur Öztürk, Fatih Köksal","doi":"10.4274/tpd.galenos.2024.16056","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2024.16056","url":null,"abstract":"<p><strong>Objective: </strong>It is known that protozoa and helminths that cause intestinal infections adversely affect human life. Changing climate and demographic and socio-economic factors worldwide necessitate the determination and updating of the incidence of these parasites. Our study aimed to retrospectively examine the distribution of intestinal parasites detected in the Parasitology Laboratory of Çukurova University Faculty of Medicine between 2017 and 2021.</p><p><strong>Methods: </strong>Parasitological examinations were performed using the native-lugol and formol-ether condensation method. Staining method (Modified Ziehl-Neelsen) and cellophane tape method were then applied to evaluate the specimens considered necessary.</p><p><strong>Results: </strong>One or more parasites were detected in 33 of 373 patients (8.8%) evaluated in the study. These were <i>Giardia intestinalis</i> at a rate of 30.5% (11/36), <i>Enterobius vermicularis</i> at a rate of 27.7% (10/36), <i>Blastocystis</i> sp. at a rate of 19.4% (7/36), <i>Entamoeba coli</i> at a rate of 11.1% (4/36), <i>Cryptosporidium</i> spp. at a rate of 8.3% (3/36) and <i>Taenia saginata</i> at a rate of 2.7% (1/36). It was determined that two patients were coinfected by <i>Entamoeba coli</i> and <i>Blastocystis</i> sp. while one patient was coinfected by <i>Entamoeba coli</i> and <i>Giardia intestinalis</i>.</p><p><strong>Conclusion: </strong>It is thought that determining the incidence of intestinal parasites, which are an important public health problem, may help guide studies for preventive health services. Although the five-year laboratory data obtained from the study do not reflect our region, it is thought that intestinal parasites maintain their importance.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 3","pages":"155-159"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Percutaneous Aspiration Injection and Re-aspiration as A Minimally Invasive Treatment for Spinal Cystic Echinococcosis: A Case Report.","authors":"Özge Metin Akcan, Kadir Yılmaz, Mustafa Gençeli, Süleyman Bakdık, Ülkü Kerimoğlu","doi":"10.4274/tpd.galenos.2024.82997","DOIUrl":"10.4274/tpd.galenos.2024.82997","url":null,"abstract":"<p><p>Cystic echinococcosis is a parasitic disease with significant importance for public health in endemic regions. Spinal cystic echinococcosis, however, is a rare form that may lead to severe complications due to its localization. In this manuscript, we presented a 16-year-old male patient who admitted with abdominal and back edema for 2 months, evaluated with preliminary diagnoses of Pott's abscess and malignant mass, subsequently diagnosed with spinal cystic echinococcosis. It was concluded that cystic echinococcosis should be considered in differential diagnosis of large cystic masses and percutaneous aspiration, injection, reaspiration method might be a safe and effective treatment option particularly for cases of complicated spinal cystic echinococcosis.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 3","pages":"191-194"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Diagnosis and Typing of <i>Cryptosporidium</i> spp. Species in Human Stools with Diarrhea.","authors":"Fatma Özkan, Anil İça","doi":"10.4274/tpd.galenos.2024.60392","DOIUrl":"10.4274/tpd.galenos.2024.60392","url":null,"abstract":"<p><strong>Objective: </strong>This study was conducted to molecularly identify and classify <i>Cryptosporidium</i> spp. in fecal samples (n=150) from patients with diarrhea received at the microbiology laboratory of a private hospital in Denizli.</p><p><strong>Methods: </strong>In this study, the positivity of <i>Cryptosporidium</i> spp. in fecal samples was investigated using direct microscopy, Kinyoun's acid-fast staining method, and Nested polymerase chain reaction (PCR) techniques. Positive PCR products were sequenced.</p><p><strong>Results: </strong>In the examined fecal samples of patients with diarrhea, no parasites were detected through direct microscopic examination. Using the Kinyoun acid-fast staining method, <i>Cryptosporidium</i> spp. was identified in 2.7% (n=4) of the samples, while Nested PCR detected it in 4.67% (n=7) of the samples. The four positive samples were sequenced using primers that amplify the 18S <i>rRNA</i> gene region. The sequencing results identified the isolates as <i>C. parvum</i>.</p><p><strong>Conclusion: </strong>Cryptosporidiosis is an important public health issue as it is a zoonotic disease caused by the <i>Cryptosporidium</i> parasite that can be transmitted from animals to humans. This study focuses on the molecular characterization of <i>Cryptosporidium</i> species detected in human fecal samples, which is significant for understanding which specific strains or species are involved in human infections. According to the findings, it is recommended that control measures be implemented to reduce the risk of exposure to <i>Cryptosporidium</i> in both humans and animals in Türkiye.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 3","pages":"184-190"},"PeriodicalIF":0.0,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142381812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}