Turkiye parazitolojii dergisi最新文献

{"title":"A Case of Nasopharyngeal Myiasis Caused by <i>Sarcophaga</i> sp","authors":"Mutalip Çiçek,&nbsp;Fatih Çakır,&nbsp;Duygu Neval Sayın İpek,&nbsp;Alican Bilden,&nbsp;Eşref Akıl","doi":"10.4274/tpd.galenos.2023.86547","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2023.86547","url":null,"abstract":"<p><p>Myiasis is a parasitic infestation of tissues and body cavities of vertebrates by <i>Diptera larvae</i>. Nasal myiasis is mostly caused by the <i>Sarcophagidae</i> and <i>Calliphoridae</i> families. Many predisposing factors play a role in the appearance of nasal myiasis. In the treatment of the disease, the use of antiseptics together with mechanical cleaning is recommended. In this report, a case of nasopharyngeal myiasis in a 75-year-old patient hospitalized in the intensive care unit is presented. Nine larvae were detected in the patient's nose. Larvae were removed from the area and were cleaned with iodine solution for three days. As a result of macroscopic and microscopic examination, the larvae were identified as the third stage of <i>Sarcophaga</i> sp. in order to prevent myiasis infestations, personnel working in places where the patient is unconscious, such as intensive care units, should daily check the patient's body cavities such as nose, mouth and ear cavities, and perform their care and cleaning.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 2","pages":"124-126"},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9553043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution of Aedes (<i>Stegomyia</i>) cretinus in Türkiye","authors":"Fatih Mehmet Şimşek,&nbsp;Sare İlknur Yavaşoğlu","doi":"10.4274/tpd.galenos.2023.72692","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2023.72692","url":null,"abstract":"<p><strong>Objective: </strong><i>Aedes cretinus</i>, a white and black stripped <i>Aedes species</i>, shares morphological similarities with <i>Aedes albopictus</i> and <i>Aedes aegypti</i> which are among the most important vectors and invasive species in the world. Due to its limited distribution and low population density, information on the biology and ecology of the species has been limited so far. This study aimed to determine distribution of Ae. <i>cretinus</i> in Türkiye.</p><p><strong>Methods: </strong>Field works have been carried out in the Mediterranean, Aegean and Marmara Regions of Türkiye. Larval samples were collected by larval dippers while adult mosquito catches were performed using the human landing catch method and CDC-light traps.</p><p><strong>Results: </strong>A total of 44 different Ae. <i>cretinus</i> populations were identified in the Mediterranean, Aegean and Marmara Regions of Türkiye. The larval specimen collected from small containers, tires, tree holes and natural small habitats. A plane (<i>Platanus orientalis</i>), walnut (<i>Juglans regia</i>), Türkiye oak (<i>Quercus cerris</i>), chestnut (<i>Castenea sativa</i>) and cedar (<i>Cedrus libani</i>) tree holes are the main larval habitats in which the specimen collected. In some localities, larvae were found together with <i>Anopheles plumbeus</i>, <i>Anopheles claviger</i> and <i>Aedes geniculatus</i> larvae in tree holes. Human landing catch method captured a greater number of females than CDC-light traps.</p><p><strong>Conclusion: </strong>This study determined the distribution of Ae. <i>cretinus</i> in Türkiye for the first time. Information on respective geographic distribution of Ae. <i>cretinus</i> is fundamental for effective control programmes. Further studies are needed to understand the biology and ecology of these species.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 2","pages":"117-123"},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9553039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Analysis of Serology and Radiology Results in Patients with Suspected Cystic Echinococcosis Through 3 Years Period","authors":"Büşra Betül Özmen Çapın,&nbsp;Barış Can,&nbsp;Meltem Kurşun,&nbsp;Canan Cimşit,&nbsp;Ayşegül Karahasan","doi":"10.4274/tpd.galenos.2023.24186","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2023.24186","url":null,"abstract":"<p><strong>Objective: </strong>Cystic echinococcosis (CE), caused by the larval stage of <i>Echinococcus granulosus</i> sensu latu, is one of the neglected zoonotic infectious diseases and Türkiye is among the endemic countries. This study was designed to analyze serology results for patients who were diagnosed as CE by clinical symptoms and radiological methods over a three-year period.</p><p><strong>Methods: </strong>Sera were analyzed for Anti-<i>E. granulosus</i> IgG by a chemiluminescence immunoassay (CLIA) (HYDATIDOSIS VIRCLIA^® IgG MONOTEST, Vircell) using the VIRCLIA^® (CLIA; Vircell, Granada, Spain) and results processed by the dedicated software. Cut-off for a positive test was ≥1.1 index value. Echinococcal cysts were detected based on ultrasonography, computed tomography, and magnetic resonance imaging.</p><p><strong>Results: </strong>A total of 244 sera were analyzed from 109 patients, during three-year-period from January 2018 to December 2020. Anti-<i>E. granulosus</i> IgG was ordered twice in 89 patients, three times in 15 patients, four times in four patients, and five times in one patient. CLIA test was positive among 41 of 109 (37.6%) patients in whom 32 (76%) had only hepatic involvement, whereas in 5 (12%) hepatic and pulmonary involvement were coexisted. The mean age of seropositive patients was 39.8 (6-75±2.72) and 61.9% of them (n=26) were female. Time intervals between sequential test orders varied from 1 day to 33 months. Eight seropositive patients turned out to be negative, and one of 66 seronegative patients became seropositive. Positive test results were converted to negative in the case of therapy or cyst inactivity.</p><p><strong>Conclusion: </strong>We may conclude that CLIA could be used as a complementary tool for CE patient follow-up.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 2","pages":"83-87"},"PeriodicalIF":0.0,"publicationDate":"2023-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9556330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Analysis of Serology and Radiology Results in Patients with Suspected Cystic Echinococcosis Through 3 Years Period.","authors":"Büşra Betül Özmen Çapın, Barış Can, Meltem Kurşun, C. Cimşit, A. Karahasan","doi":"10.4274/tpd.galenos.2022.24186","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.24186","url":null,"abstract":"OBJECTIVE\u0000Cystic echinococcosis (CE), caused by the larval stage of Echinococcus granulosus sensu latu, is one of the neglected zoonotic infectious diseases and Türkiye is among the endemic countries. This study was designed to analyze serology results for patients who were diagnosed as CE by clinical symptoms and radiological methods over a three-year period.\u0000\u0000\u0000METHODS\u0000Sera were analyzed for Anti-E. granulosus IgG by a chemiluminescence immunoassay (CLIA) (HYDATIDOSIS VIRCLIA® IgG MONOTEST, Vircell) using the VIRCLIA® (CLIA; Vircell, Granada, Spain) and results processed by the dedicated software. Cut-off for a positive test was ≥1.1 index value. Echinococcal cysts were detected based on ultrasonography, computed tomography, and magnetic resonance imaging.\u0000\u0000\u0000RESULTS\u0000A total of 244 sera were analyzed from 109 patients, during three-year-period from January 2018 to December 2020. Anti-E. granulosus IgG was ordered twice in 89 patients, three times in 15 patients, four times in four patients, and five times in one patient. CLIA test was positive among 41 of 109 (37.6%) patients in whom 32 (76%) had only hepatic involvement, whereas in 5 (12%) hepatic and pulmonary involvement were coexisted. The mean age of seropositive patients was 39.8 (6-75±2.72) and 61.9% of them (n=26) were female. Time intervals between sequential test orders varied from 1 day to 33 months. Eight seropositive patients turned out to be negative, and one of 66 seronegative patients became seropositive. Positive test results were converted to negative in the case of therapy or cyst inactivity.\u0000\u0000\u0000CONCLUSION\u0000We may conclude that CLIA could be used as a complementary tool for CE patient follow-up.","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"1 1","pages":"83-87"},"PeriodicalIF":0.0,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88647351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Evaluation of Toxoplasma gondii Serological Test Results of Trakya University Health Center for Medical Research and Practice","authors":"Canan Eryıldız,&nbsp;Berrak Kaplan Çakmakçı,&nbsp;Feza İrem Aldı,&nbsp;Nermin Şakru","doi":"10.4274/tpd.galenos.2022.25743","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.25743","url":null,"abstract":"<p><strong>Objective: </strong><i>Toxoplasma gondii</i> (<i>T. gondii</i>) is an obligate intracellular parasite distributed worldwide. Serological tests investigating antibodies specific to <i>T. gondii</i> are widely used in diagnosis. The aim of this study was to evaluate the results of anti-<i>T. gondii</i> IgG, anti-<i>T. gondii</i> IgM, and anti-<i>T. gondii</i> IgG avidity tests, which were sent to the Serology Laboratory of Trakya University Health Center for Medical Research and Practice, retrospectively.</p><p><strong>Methods: </strong>Anti-<i>T. gondii</i> IgM, anti-<i>T. gondii</i> IgG, and anti-<i>T. gondii</i> IgG avidity tests were studied by enzyme-linked fluorescent assay or electrochemiluminescence immunoassay method between January 2012 and December 2021. The test results were evaluated retrospectively from laboratory records.</p><p><strong>Results: </strong>Of 18,659 serum samples were studied for anti-<i>T. gondii</i> IgG, 5,127 (27.5%) samples were positive, whereas 721 (3.4%) of 21,108 samples were positive for anti-<i>T. gondii</i> IgM. Of the 593 serum samples tested for IgG avidity, 206 (34.7%) samples had low avidity, 118 (19.9%) had borderline, and 269 (45.4%) had high avidity.</p><p><strong>Conclusion: </strong>Our study, compatible with other studies, showed that seropositivity is high in our region, which is not negligible. Especially in women of reproductive age population, <i>T. gondii</i> should be considered in suspected clinical cases.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"6-10"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9178458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Prevalence of Larval Stages of <i>Fasciola hepatica</i> in <i>Lymnaea stagnalis</i> Species Snails in the Vicinity of the Ağrı Province","authors":"Ahmet Hakan Ünlü, Rahmi Yıldız, Selahattin Aydemir, Abdurrahman Ekici","doi":"10.4274/tpd.galenos.2022.52714","DOIUrl":"10.4274/tpd.galenos.2022.52714","url":null,"abstract":"<p><strong>Objective: </strong><i>Lymnaea stagnalis</i> known as the great pond snail, is one of the intermediate hosts of <i>Fasciola hepatica</i>, a zoonotic parasite. In this study, it was aimed to determine the larval forms of <i>F. hepatica</i> by polymerase chain reaction (PCR) in <i>L. stagnalis</i> species snails collected from the vicinity of Ağrı province.</p><p><strong>Methods: </strong>In this study, 150 <i>L. stagnalis</i> snails were collected from the Ağrı province. The freshwater snails brought to the laboratory were dissected, then their soft tissues were examined under a microscope. DNA extraction was performed on the dissected snails. After DNA extraction, PCR was performed using primers targeting the cytochrome c oxidase subunit 1 gene region.</p><p><strong>Results: </strong>In the microscopic examination, larval forms of <i>F. hepatica</i> could not be detected. However, it was concluded that two (1.3%) <i>L. stagnalis</i> freshwater snails were infected with the larval forms of <i>F. hepatica</i> in the PCR.</p><p><strong>Conclusion: </strong>It was determined that <i>L. stagnalis</i> served as an intermediate host to <i>F. hepatica</i> in the study area.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"34-37"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9178460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Importance of Antioxidant Enzymes and Oxidative Stress in Human Fascioliasis","authors":"Zeynep Taş Cengiz, Hasan Yılmaz, Yunus Emre Beyhan, Abdurrahman Ekici, Mutalip Çiçek, Selahattin Aydemir","doi":"10.4274/tpd.galenos.2022.07088","DOIUrl":"10.4274/tpd.galenos.2022.07088","url":null,"abstract":"&lt;p&gt;&lt;strong&gt;Objective: &lt;/strong&gt;The aim of this study was to determine the levels of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT), and malondialdehyde (MDA) in the patients infected with &lt;i&gt;Fasciola hepatica&lt;/i&gt; and establish whether these parameters differ among the patients with fascioliasis.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Methods: &lt;/strong&gt;The patient group consisted of 140 individuals with &lt;i&gt;F. hepatica&lt;/i&gt; seropositive; the control group consisted of 140 healthy individuals who tested negative for this parasite and had no other diseases. The patient group consisted of individuals with no chronic diseases other than fascioliasis; in both the patient and the control groups, the subjects had no unhealthy habits such as smoking and alcohol consumption, etc. The blood samples taken to diagnose fascioliasis were evaluated by the ELISA method. The samples were studied according to the kit procedures for SOD, CAT, GPx and MDA markers.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Results: &lt;/strong&gt;In this study, 43.6% of 140 individuals in the patient group infected with &lt;i&gt;F. hepatica&lt;/i&gt; had CAT (p=0.001), 35% had GPx (p=0.001), 12.9% had SOD (p=0.002), 90.7% had MDA (p=0.001). There was found a statistically significant difference between the patient and the control group in terms of the positivity of these four parameters.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Conclusion: &lt;/strong&gt;As a result, a statistically significant relationship was found between the increase in the SOD, GPx, CAT, and MDA levels and fascioliasis. The high rate of MDA revealed that oxidative stress occurred in patients with fascioliasis, resulting in an increased activity of SOD, GPx, and CAT.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Amaç: &lt;/strong&gt;Bu çalışmanın amacı, &lt;i&gt;Fasciola hepatica&lt;/i&gt; ile enfekte hastalarda süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx), katalaz (CAT) ve malondialdehit (MDA) düzeylerini belirlemek ve fascioliasisli hastalarda bu parametrelerde farklılık meydana gelip gelmediğini ortaya koymaktır.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Yöntemler: &lt;/strong&gt;Hasta grubu, &lt;i&gt;F. hepatica&lt;/i&gt; pozitif olan 140 hastadan; kontrol grubu ise bu parazit yönünden negatif bulunan ve başka herhangi bir hastalığı bulunmayan 140 sağlıklı kişiden oluşturuldu. Hasta grubuna fascioliasis dışında herhangi bir kronik hastalığı olmayan ve hem hasta hem de kontrol grubuna sigara, alkol kullanımı olmayan kişiler dahil edildi. Hastalarda fascioliasis pozitifliğini belirlemek için alınan kan örnekleri ELISA yöntemi ile çalışıldı. Serum SOD, CAT, GPx ve MDA düzeyleri ELISA yöntemi ile değerlendirildi.&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Bulgular: &lt;/strong&gt;Bu çalışmada &lt;i&gt;F. hepatica&lt;/i&gt; ile enfekte hasta grubundaki 140 kişinin %43,6’sında CAT (p=0,001), %35’inde GPx (p=0,001), %12,9’unda SOD (p=0,002) ve %90,7 MDA pozitifliği saptandı. Bu dört parametrenin pozitifliği açısından hasta ve kontrol grubu arasında istatistiksel olarak anlamlı fark bulundu (p=0,001).&lt;/p&gt;&lt;p&gt;&lt;strong&gt;Sonuç: &lt;/strong&gt;Sonuç olarak SOD, GPx, CAT ve MDA düzeyindeki artış ile fascioliasis arasında istatistik olarak an","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"38-41"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9121635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Report of <i>Aphanurus stossichii</i> (Digenea: Hemiuridae) from <i>Engraulis encrasicolus</i> on the Turkish Coast of the Black Sea, with Light and Scanning Electron Microscopic Observations","authors":"Türkay Öztürk,&nbsp;Arzu Güven","doi":"10.4274/tpd.galenos.2022.30301","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.30301","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to investigate in detail the morphological features of the digenean parasite <i>Aphanurus stossichii</i> isolated from the European anchovy <i>Engraulis encrasicolus</i> using light and scanning electron microscopy (SEM).</p><p><strong>Methods: </strong>The specimens of <i>A. stossichii</i> were obtained from the pharynx and stomach of the European <i>E. encrasicolus</i> caught by commercial fishing vessels in the Black Sea. Parasites were killed in a hot normal saline solution, preserved in to 70% ethanol for light microscopic (LM) studies, and in 2.5% glutaraldehyde for SEM. The morphological diagnostic features of <i>A. stossichii</i> were studied in detail under both LM and SEM.</p><p><strong>Results: </strong>The morphological characteristics of the examined adult <i>A. stossichii</i> specimens were found to be similar to the original descriptions describing the basic characteristics of forebody and hindbody shape, the position and shape of the vitellarium, ovary, and and testes, and the shapes of oral and ventral sucker. The measurement data for all morphological diagnostics were provided; photomicrographs of each part of the parasite were presented. Infection prevalence, mean intensity and mean abundance values were 8.89%, 4.5 and 0.4, respectively.</p><p><strong>Conclusion: </strong>All available records of <i>A. stossichii</i> morphology are based on light microscope, this is the first study to identification the morphological features of the parasite with SEM. This research is the first on <i>A. stossichii</i> presence in <i>E. encrasicolus</i> on the Turkish Black Sea coast.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"42-48"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9178461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of <i>Toxoplasma</i>, <i>Rubella</i> and <i>Cytomegalovirus</i> in Women of Fertility Age in Our Region","authors":"İlkay Bahçeci,&nbsp;Esra Karaca,&nbsp;Ömer Faruk Duran,&nbsp;Duygu Aksoy,&nbsp;Yunus Emre İbik,&nbsp;Umut Buğra Kırcı","doi":"10.4274/tpd.galenos.2022.07379","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.07379","url":null,"abstract":"<p><strong>Objective: </strong><i>Toxoplasma gondii</i> (<i>T. gondii</i>), <i>Rubella</i> and <i>Cytomegalovirus</i> (CMV) infections can cause severe morbidity in the fetus when transmissed during pregnancy. In our study, it was aimed to examine the seropositivity rates for <i>T. gondii</i>, <i>Rubella</i> and CMV infections in women of childbearing age who applied to our hospital.</p><p><strong>Methods: </strong>Anti-<i>Toxoplasma</i> IgG, anti-<i>Toxoplasma</i> IgM, anti-<i>Rubella</i> IgG, anti-<i>Rubella</i> IgM, anti-CMV IgG and anti-CMV were studied in women of childbearing age (18-49 years old) who applied to our hospital's outpatient clinics between January 2018 and December 2020. The tests were performed in our microbiology laboratory using the ELISA method on Architect i2000 (Abbott, USA) and COBAS e601 (Roche, Germany) devices.</p><p><strong>Results: </strong>As a result of the data obtained, the percentages of IgM and IgG positivity for anti-<i>Toxoplasma</i> were calculated as 1.4% and 30.9%, respectively. Anti-<i>Rubella</i> IgM positivity was 0.7%, anti-<i>Rubella</i> IgG positivity was 91%, anti-CMV IgG positivity was 98.8%, and anti-CMV IgM positivity was 2%.</p><p><strong>Conclusion: </strong>Having its own seroprevalence for each region has is important in terms of planning pregnancy screenings. The seropositivity rates in our region are in line with other studies in the country. Since CMV seropositivity is very high in the population and there is no effective treatment or vaccine, screening may not be not necessary. <i>T. gondii</i> and <i>Rubella</i> screenings can be recommended due to the lower immunity rates and the availability of vaccine and treatment options.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"11-15"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9178457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization of Mitochondrial Cytochrome <i>c</i> oxidase subunit 1 (<i>Cox1</i>) gene from <i>Trichostrongylus</i> Species (Nematoda: Trichostrongylidae) in Northern Iran","authors":"Meysam Sharifdini,&nbsp;Elham Hajialilo,&nbsp;Hedayat Hosseinnezhad,&nbsp;Mohammad Ali Mohammadi","doi":"10.4274/tpd.galenos.2022.16878","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.16878","url":null,"abstract":"<p><strong>Objective: </strong>The objective of the present study was to identify <i>Trichostrongylus</i> species by molecular analysis and also phylogenetic relationships of <i>Trichostrongylus</i> species by mitochondrial Cytochrome <i>c</i> oxidase subunit 1 (<i>Cox1</i>) gene in Guilan province, northern Iran.</p><p><strong>Methods: </strong>Abomasum and duodenum contents of 144 livestock were collected from sheep, goats, and cattle in Guilan province. Morphological survey was performed for initial screening. Total DNA was extracted, and the partial region of <i>Cox1</i> gene was amplified and sequenced. Genetic diversity was calculated and phylogenetic analysis of the data on nucleotide sequence was conducted by MEGA7 software.</p><p><strong>Results: </strong>Three species of <i>Trichostrongylus</i> including <i>T. colubriformis</i>, <i>T. vitrinus</i>, and <i>T. axei</i> were identified by morphological characteristics. The genetic divergence within the species in the present study was observed for <i>T. axei</i> (0-2.5%), <i>T. colubriformis</i> (0.77%), and <i>T. vitrinus</i> (0%). The mean inter-species difference between the three species of <i>Trichostrongylus</i> obtained in this study was 14.4-15.4%.</p><p><strong>Conclusion: </strong>The <i>Cox1</i> sequences of the members of <i>Trichostrongylus</i> spp. were highly variable and this could be used as a valuable measure to achieve a proper assessment on biodiversity. Sequence data generation from other species of <i>Trichostrongylus</i> will be needed to reconstruct the phylogenetic relationships of this genus of nematodes.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"28-33"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9121634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}