Turkiye parazitolojii dergisi最新文献

{"title":"First Report of <i>Aphanurus stossichii</i> (Digenea: Hemiuridae) from <i>Engraulis encrasicolus</i> on the Turkish Coast of the Black Sea, with Light and Scanning Electron Microscopic Observations","authors":"Türkay Öztürk,&nbsp;Arzu Güven","doi":"10.4274/tpd.galenos.2022.30301","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.30301","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to investigate in detail the morphological features of the digenean parasite <i>Aphanurus stossichii</i> isolated from the European anchovy <i>Engraulis encrasicolus</i> using light and scanning electron microscopy (SEM).</p><p><strong>Methods: </strong>The specimens of <i>A. stossichii</i> were obtained from the pharynx and stomach of the European <i>E. encrasicolus</i> caught by commercial fishing vessels in the Black Sea. Parasites were killed in a hot normal saline solution, preserved in to 70% ethanol for light microscopic (LM) studies, and in 2.5% glutaraldehyde for SEM. The morphological diagnostic features of <i>A. stossichii</i> were studied in detail under both LM and SEM.</p><p><strong>Results: </strong>The morphological characteristics of the examined adult <i>A. stossichii</i> specimens were found to be similar to the original descriptions describing the basic characteristics of forebody and hindbody shape, the position and shape of the vitellarium, ovary, and and testes, and the shapes of oral and ventral sucker. The measurement data for all morphological diagnostics were provided; photomicrographs of each part of the parasite were presented. Infection prevalence, mean intensity and mean abundance values were 8.89%, 4.5 and 0.4, respectively.</p><p><strong>Conclusion: </strong>All available records of <i>A. stossichii</i> morphology are based on light microscope, this is the first study to identification the morphological features of the parasite with SEM. This research is the first on <i>A. stossichii</i> presence in <i>E. encrasicolus</i> on the Turkish Black Sea coast.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"42-48"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9178461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of <i>Toxoplasma</i>, <i>Rubella</i> and <i>Cytomegalovirus</i> in Women of Fertility Age in Our Region","authors":"İlkay Bahçeci,&nbsp;Esra Karaca,&nbsp;Ömer Faruk Duran,&nbsp;Duygu Aksoy,&nbsp;Yunus Emre İbik,&nbsp;Umut Buğra Kırcı","doi":"10.4274/tpd.galenos.2022.07379","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.07379","url":null,"abstract":"<p><strong>Objective: </strong><i>Toxoplasma gondii</i> (<i>T. gondii</i>), <i>Rubella</i> and <i>Cytomegalovirus</i> (CMV) infections can cause severe morbidity in the fetus when transmissed during pregnancy. In our study, it was aimed to examine the seropositivity rates for <i>T. gondii</i>, <i>Rubella</i> and CMV infections in women of childbearing age who applied to our hospital.</p><p><strong>Methods: </strong>Anti-<i>Toxoplasma</i> IgG, anti-<i>Toxoplasma</i> IgM, anti-<i>Rubella</i> IgG, anti-<i>Rubella</i> IgM, anti-CMV IgG and anti-CMV were studied in women of childbearing age (18-49 years old) who applied to our hospital's outpatient clinics between January 2018 and December 2020. The tests were performed in our microbiology laboratory using the ELISA method on Architect i2000 (Abbott, USA) and COBAS e601 (Roche, Germany) devices.</p><p><strong>Results: </strong>As a result of the data obtained, the percentages of IgM and IgG positivity for anti-<i>Toxoplasma</i> were calculated as 1.4% and 30.9%, respectively. Anti-<i>Rubella</i> IgM positivity was 0.7%, anti-<i>Rubella</i> IgG positivity was 91%, anti-CMV IgG positivity was 98.8%, and anti-CMV IgM positivity was 2%.</p><p><strong>Conclusion: </strong>Having its own seroprevalence for each region has is important in terms of planning pregnancy screenings. The seropositivity rates in our region are in line with other studies in the country. Since CMV seropositivity is very high in the population and there is no effective treatment or vaccine, screening may not be not necessary. <i>T. gondii</i> and <i>Rubella</i> screenings can be recommended due to the lower immunity rates and the availability of vaccine and treatment options.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"11-15"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9178457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization of Mitochondrial Cytochrome <i>c</i> oxidase subunit 1 (<i>Cox1</i>) gene from <i>Trichostrongylus</i> Species (Nematoda: Trichostrongylidae) in Northern Iran","authors":"Meysam Sharifdini,&nbsp;Elham Hajialilo,&nbsp;Hedayat Hosseinnezhad,&nbsp;Mohammad Ali Mohammadi","doi":"10.4274/tpd.galenos.2022.16878","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.16878","url":null,"abstract":"<p><strong>Objective: </strong>The objective of the present study was to identify <i>Trichostrongylus</i> species by molecular analysis and also phylogenetic relationships of <i>Trichostrongylus</i> species by mitochondrial Cytochrome <i>c</i> oxidase subunit 1 (<i>Cox1</i>) gene in Guilan province, northern Iran.</p><p><strong>Methods: </strong>Abomasum and duodenum contents of 144 livestock were collected from sheep, goats, and cattle in Guilan province. Morphological survey was performed for initial screening. Total DNA was extracted, and the partial region of <i>Cox1</i> gene was amplified and sequenced. Genetic diversity was calculated and phylogenetic analysis of the data on nucleotide sequence was conducted by MEGA7 software.</p><p><strong>Results: </strong>Three species of <i>Trichostrongylus</i> including <i>T. colubriformis</i>, <i>T. vitrinus</i>, and <i>T. axei</i> were identified by morphological characteristics. The genetic divergence within the species in the present study was observed for <i>T. axei</i> (0-2.5%), <i>T. colubriformis</i> (0.77%), and <i>T. vitrinus</i> (0%). The mean inter-species difference between the three species of <i>Trichostrongylus</i> obtained in this study was 14.4-15.4%.</p><p><strong>Conclusion: </strong>The <i>Cox1</i> sequences of the members of <i>Trichostrongylus</i> spp. were highly variable and this could be used as a valuable measure to achieve a proper assessment on biodiversity. Sequence data generation from other species of <i>Trichostrongylus</i> will be needed to reconstruct the phylogenetic relationships of this genus of nematodes.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"28-33"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9121634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of <i>Toxoplasma gondii</i> Seroprevalence in People Keeping Cats and Not Keeping Cats at Their Home in Sivas","authors":"İbrahim Özmen,&nbsp;Ahmet Duran Ataş","doi":"10.4274/tpd.galenos.2022.73745","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.73745","url":null,"abstract":"Objective\u0000Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan and its definitive host is the Felidae family. Toxoplasmosis can be transmitted to humans in many ways. The purpose of the study was to investigate the anti-Toxoplasma IgM and anti-Toxoplasma IgG seropositivity with the ELISA method in people who have cats at home and do not have cats at home, and to reveal the possible relationship between toxoplasmosis and people who keep/contact cats for a long time for any reason at home.\u0000\u0000\u0000Methods\u0000Between March 2021 and June 2021, blood samples were taken from 91 people who had a cat in their home for at least a year and 91 people who had never had a cat or had no contact with a cat, in Sivas province. Anti-Toxoplasma IgM and anti-Toxoplasma IgG antibodies were investigated in serum samples by the ELISA method. Age, gender, and other socio-demographic criteria were not considered.\u0000\u0000\u0000RESULTSResults\u0000Because of the study, all samples were found to be negative for anti-Toxoplasma IgM. Anti-Toxoplasma IgG seropositivity was detected in 20 (22.0%) of those who had cats at home and 40 (44.0%) of those without cats at home. There was no statistically significant difference between the two groups in terms of anti-Toxoplasma IgM seropositivity. However, anti-Toxoplasma IgG seropositivity was found to be statistically significant (p=0.002) (p<0.01).\u0000\u0000\u0000Conclusion\u0000Because of the study, anti-Toxoplasma IgG positivity was found to be higher in those who did not feed/contact cats at home and it was statistically significant. It brings to mind that the reason for the high rate of seropositivity in those without cats at home, may not be only the oocysts excreted from cats, but also the transmission by other non-cat transmission routes may still be important.","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"1-5"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9474122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gastro-intestinal and Respiratoric System Helminths of Domestic Geese in Samsun and Districts","authors":"Yılmaz Parlak,&nbsp;Ali Tümay Gürler","doi":"10.4274/tpd.galenos.2022.02411","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.02411","url":null,"abstract":"<p><strong>Objective: </strong>This research was carried out to determine the digestive and respiratory system helminths of domestic geese collected from Canik, Çarşamba, Havza, Kavak, Terme, and Tekkeköy districts representing Samsun province.</p><p><strong>Methods: </strong>Within the scope of the study, the digestive and respiratory system organs of 64 domestic geese were collected. Organ sets were taken separately, and the contents of each organ were examined.</p><p><strong>Results: </strong>According to macroscopic and microscopic examination, 5 different helminth species were detected in 53 (82.8%) geese: <i>Baruscapillaria obsignata</i> (59.4%), <i>B. anseris</i> (32.8%), <i>Amidostomum anseris</i> (9.4%), Trichostrogylus tenuis (1.6%), and <i>Heterakis</i> sp. (1.6%).</p><p><strong>Conclusion: </strong>At the end of the study, all helminths were found in the digestive system and all of them were nematods. In conclusion, it has been predicted that nematodes that settle in the digestive system of geese are frequently encountered and this may be a problem for goose breeders.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"49-52"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9178462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of Anti-<i>Toxoplasma gondii</i> Antibodies in the Hemodialysis Patients with ELISA Method","authors":"Şehriban Yürektürk,&nbsp;Hasan Yılmaz,&nbsp;Zeynep Taş Cengiz","doi":"10.4274/tpd.galenos.2022.87587","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.87587","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to determine the seroprevalence of <i>Toxoplasma gondii</i> in hemodialysis (HD) patients and to reveal the importance of toxoplasmosis as a risk factor in these patients.</p><p><strong>Methods: </strong>The study was carried out between 26.12.2013 and 01.01.2016 at Van Yüzüncü University Dursun Odabaş the Medical Center on patients with chronic renal failure who entered HD. As the patient group in the study, 150 patients with chronic renal failure who underwent HD; as the control group, 50 people without any known chronic disease and who did not receive any immunosuppressive therapy were included. The ELISA method was used to determine anti-<i>T. gondii</i> IgG and IgM antibody levels. A questionnaire including risk factors that may cause the transmission of <i>T. gondii</i> was applied to the patient and control groups.</p><p><strong>Results: </strong>In the study, 89 out of total 150 HD patients (59.3%) were found anti-<i>T. gondii</i> IgG antibody seropositive and 4 were (2.7%) anti-<i>T. gondii</i> IgM antibody seropositive. Fourteen of 50 healthy individuals in the group (28%) were anti-<i>T. gondii</i> IgG antibody positive, while none in this group was anti-<i>T. gondii</i> IgM antibody positive. Statistical analysis demonstrated there were separate significant correlations between both anti-<i>T. gondii</i> IgG (p<0.01) and anti-<i>T. gondii</i> IgM antibody (p<0.05) frequencies with chronic renal failure. While there were no statistically significant differences in the prevalence of anti-<i>T. gondii</i> IgG antibody identification based on gender and age groups, there were significant differences between the prevalence of anti-<i>T. gondii</i> IgM antibody based on both gender (p<0.05) and age groups (p<0.05). Some living conditions and habits of the patient group were evaluated statistically, and a significant correlation (p<0.05) was found between eating only raw meatballs and toxoplasmosis seropositivity.</p><p><strong>Conclusion: </strong>As a result, it was understood that the physicians who monitor of HD patients should assess toxoplasmosis among the risk factors.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"16-21"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9178455","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monitoring the Trends in Intestinal Parasite Frequencies; 2018 and 2022 Data","authors":"Orçun Zorbozan,&nbsp;Nevin Turgay","doi":"10.4274/tpd.galenos.2023.66376","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2023.66376","url":null,"abstract":"<p><strong>Objective: </strong>Monitoring intestinal parasite frequencies is effective on diagnosis, treatment, and prevention strategies to be developed against these parasites. In this study, it was aimed to reveal the parasite species and frequency data of stool samples in parasitology direct diagnosis laboratory.</p><p><strong>Methods: </strong>Stool parasitological examination results were obtained retrospectively from our laboratory internal quality control data tables. Data belonging to the year 2018 and 2022 were compared retrospectively.</p><p><strong>Results: </strong>Annual parasites detected in stool samples were 388 of 4.518, and 710 of 3.537, in 2018 and 2022, respectively. Frequency of parasite detection in stool samples was found to be significantly higher in 2022 (p<0.0001). Number of stools with more than one parasite was 12 and 30 in 2018 and 2022, respectively. Incidence of infection with more than one parasite was significantly higher in 2022 (p=0.0003). Five most common parasite species were <i>Blastocystis</i> spp., <i>Enterobius vermicularis</i>, <i>Cryptosporidium</i> spp., <i>Giardia</i> intestinalis and Entamoeba histolytica in 2018, respectively; and <i>Cryptosporidium</i> spp., <i>Blastocystis</i> spp., <i>Cyclospora</i> spp., <i>Entamoeba dispar</i> and <i>Giardia</i> intestinalis, in 2022, respectively. <i>Cryptosporidium</i> spp., <i>Cyclospora</i> spp. and <i>Entamoeba dispar</i> increased significantly, while <i>Blastocystis</i> spp. and <i>Enterobius vermicularis</i> decreased significantly, in 2022.</p><p><strong>Conclusion: </strong>According to the data obtained, causative agents for intestinal parasitic infections were protozoans, especially <i>Cryptosporidium</i> spp. It has been concluded that tightening the measures for protection of water with one health approach and improving the education and habits of society on personal hygiene and food safety can be effective in reducing the frequency of intestinal parasite infections in our region.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"59-63"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9121636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of Ecto and Gastrointestinal Parasites of <i>Rattus rattus</i> in Mazandaran Province, North of Iran","authors":"Ahmad Daryani,&nbsp;Afsaneh Amouei,&nbsp;Abdol Sattar Pagheh,&nbsp;Mehdi Sharif,&nbsp;Shahabeddin Sarvi,&nbsp;Mohammad Taghi Rahimi,&nbsp;Fatemeh Rezaei","doi":"10.4274/tpd.galenos.2022.85570","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.85570","url":null,"abstract":"<p><strong>Objective: </strong>Rodents act as reservoir hosts and are an important potential source for many zoonotic pathogens such as parasites, which pose a public health risk to humans. Therefore, it is necessary to investigate the prevalence of parasites among rodents.</p><p><strong>Methods: </strong>A total of 118 <i>Rattus rattus</i> were captured in Mazandaran province, north of Iran, using snap live traps. Various samples were collected from feces and each rat was combed with a fine-tooth comb to extricate any ectoparasite. Fecal specimens were examined by direct wet mounting, formalin-ether concentration, modified acid-fast, and trichrome staining methods.</p><p><strong>Results: </strong>The overall prevalence of gastrointestinal parasites in the examined rats was 75.4%. <i>Cryptosporidium</i> spp. (30.5%) were the most prevalent protozoan, followed by <i>Giardia</i> spp. (20.3%), <i>Entamoeba muris</i> (13.5%), <i>Trichomonas muris</i> (10.1%), and <i>Spironucleus muris</i> (3.3%). Regarding helminths' eggs, <i>Syphacia obvelata</i> (24.5%), <i>Hymenolepis diminuta</i> (10.1%), and <i>Trichuris muris</i> (9.3%) had the highest prevalence, respectively. Furthermore, 3060 ectoparasites collected from 102 rodents were infested with lice (40% <i>Polyplax</i> spp.), mites (33.3%), and flea (16.1% <i>Xenopsylla cheopis</i> and 10.6% <i>Xenopsylla astia</i>).</p><p><strong>Conclusion: </strong>According to the results of this study, the prevalence of ecto and gastrointestinal parasites in the collected rats in the area being studied was remarkably high. Additionally, <i>Rattus rattus</i> can be considered a potential risk to human health.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"53-58"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9121633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prevalence of <i>Haemogregarina stepanowi</i> and Assessment of Some Risk Factors in <i>Mauremys rivulata</i> (Valenciennes, 1833) Freshwater Turtles (Testudines: Geoemydidae)","authors":"Onur Ceylan,&nbsp;Çiğdem Gül,&nbsp;Nurşen Çördük,&nbsp;Nurcihan Hacıoğlu Doğru,&nbsp;Murat Tosunoğlu","doi":"10.4274/tpd.galenos.2022.38257","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.38257","url":null,"abstract":"Objective\u0000The Balkan terrapin, Mauremys rivulata, is a freshwater turtle. This reptile is exposed to many environmental pollutants and some infectious agents, including Haemogregarina stepanowi parasite. This study was conducted to determine the microscopic prevalence of haemogregarine infection in M. rivulata caught in three different localities (Bozcaada, Gökçeada, and Dardanos) in Çanakkale province of Turkey, and assessment of some risk factors.\u0000\u0000\u0000Methods\u0000Twenty-four blood samples were collected, thin blood smears were prepared, and the presence of haemogregarine parasites microscopically was screened. Water samples were also taken from the habitats, and these samples were analyzed physiochemically and microbiologically.\u0000\u0000\u0000Results\u0000Morphological identification was made by detecting the sausage-shaped intra-cytoplasmic developmental stages of H. stepanowi, and thirteen of twenty-four turtles (54.2%) were found to be infected. The prevalence of H. stepanowi was detected as the highest (90.0%) in the Gökçeada district, where the water pollution is higher than in the other localities. A statistically significant relationship was observed between the distribution of the infection and the gender of the turtles, the temperature of the water, the number of faecal coliforms in water and the amount of dissolved oxygen in the water. A statistically significant difference was found between the localities in terms of the prevalence of H. stepanowi infection, and the infection was primarily detected in the Gökçeada district.\u0000\u0000\u0000Conclusion\u0000This study has significance in providing information regarding haemoparasitic diseases of freshwater turtle, M. rivulata, in Turkey.","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"47 1","pages":"22-27"},"PeriodicalIF":0.0,"publicationDate":"2023-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9178459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of <i>Pneumocystis jirovecii</i> in Lung Cancer Patients with the Nested PCR Method","authors":"Ahmed Galip Halidi,&nbsp;Merhamet Ölçen,&nbsp;Esra Gürbüz,&nbsp;Abdurrahman Ekici,&nbsp;Selahattin Aydemir,&nbsp;Hasan Yılmaz","doi":"10.4274/tpd.galenos.2022.44153","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2022.44153","url":null,"abstract":"<p><strong>Objective: </strong><i>Pneumocystis jirovecii (P. jirovecii)</i> is an opportunistic pathogen in humans. Early diagnosis and optimal treatment of patients with <i>P. jirovecii</i> pneumonia (PJP) remains a key priority. This study investigated <i>P. jirovecii</i> in patients with lung cancer using the nested-polymerase chain reaction (PCR) method and examined the relationship between <i>P. jirovecii</i> and clinical findings.</p><p><strong>Methods: </strong>The study included 60 patients with lung cancer and 30 patients without lung cancer. The bronchoalveolar lavage (BAL) fluid samples of these 90 individuals were taken for diagnostic purposes in the University of Health Sciences Turkey, Van Training and Research Hospital, Clinic of Chest Diseases. Patient information was recorded. After DNA isolation from the BAL fluid samples taken from patients, the nested-PCR protocol for amplification of mtLSUrRNA in <i>P. jirovecii</i> was performed.</p><p><strong>Results: </strong><i>P. jirovecii</i> DNA was detected in 40 (66.67%) of the lung cancer patients included in the study and in six (20%) patients without lung cancer, that is, in 46 (51.11%) patients. The rate of nested-PCR positivity in the lung cancer group was significantly higher than that in the non-lung cancer group (p=0.0001). Additionally, a statistically significant correlation was found between anorexia and weight loss, fever and sputum <i>P. jirovecii</i> positivity in patients with lung cancer (p<0.005).</p><p><strong>Conclusion: </strong>These findings suggest that lung cancer patients should be evaluated for PJP.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":" ","pages":"276-280"},"PeriodicalIF":0.0,"publicationDate":"2022-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40490616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}