Turkiye parazitolojii dergisi最新文献

{"title":"Clinical Features and Quality of Life in Patients with Scabies.","authors":"Münevver Güven, Aybüke Parlakdağ Kılcıoğlu","doi":"10.4274/tpd.galenos.2025.70894","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.70894","url":null,"abstract":"<p><strong>Objective: </strong>Scabies is a contagious cutaneous infestation characterized by intense itching. In this study, we aimed to evaluate the clinical features of scabies and the effect of scabies on quality of life, and the change in quality of life with treatment.</p><p><strong>Methods: </strong>This prospective and cross-sectional study included 40 adults patients with classical scabies. Detailed skin examinations of the patients were performed. Dermatological life quality index (DLQI) was used to evaluate quality of life in patients with scabies. Visual analog scale (VAS) was used to investigate the severity of nocturnal itching and itch-related sleep disturbance. VAS levels and DLQI scores of the patients were evaluated at baseline (week 0) and after treatment (weeks 2 and 4).</p><p><strong>Results: </strong>The most common lesion in patients was tunnel (100%), followed by papule (97.5%), crusted papule (85%) and excoriation (77.5%). The abdomen (90%) was the most common area of lesions, followed by hands (87.5%) and forearm (85%). It was determined that the quality of life of 75% of the patients was moderate to extremely large affected. There was a significant improvement in average DLQI scores of patients after treatment compared to before treatment (p<0.001, week 0: 11.50±7.81, week 2: 5.50±4.68, week 4: 1.05±2.08). The baseline VAS values of the patients for nocturnal itching and itch-related sleep disturbance were 7.22±2.80 and 6.30±3.60, respectively. A statistically significant improvement was detected in these values in the week 2 and week 4 (p<0.001). Thirty-nine of the patients were given 10% sulfur ointment (3 consecutive days) and 35 of these patients (89.7%) recovered.</p><p><strong>Conclusion: </strong>Scabies has significant impact on quality of life in the vast majority of patients. Itch-related sleep disturbance is common in patients with scabies. Sulfur is an effective treatment choice for scabies.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 1","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of New Candidate Inhibitors Able to Prevent Erythrocyte Invasion in Malaria by Drug Screening.","authors":"Ahmet Burak Doğanoğlu, Vildan Enisoğlu Atalay","doi":"10.4274/tpd.galenos.2025.37928","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.37928","url":null,"abstract":"<p><strong>Objective: </strong>In Nowadays malaria still remains the parasitic disease causing the highest number of deaths, accounting for 619,000 fatalities. The <i>Plasmodium</i> parasites that cause malaria have separate life cycles in both humans and female Anopheles mosquitoes, existing in various forms throughout this process. The main reason for observing the disease is that merozoites sustain their existence by invading erythrocytes. Existing drugs affect the parasite's ability to digest hemoglobin. Drug resistance is also involved in this process. In this study, have been focused to develop new drug candidate molecules for evade drug resistance. To evade drug resistance, the aim was to prevent merozoites from invading erythrocytes.</p><p><strong>Methods: </strong>The invasion of merozoites into erythrocytes consists of several stages: Attachment, deformation, apical junction formation, and tight junction formation. For this purpose, the docking calculations have been done between the invasion proteins such as MSP1, pvDBP, phRH5, AMA1 and candidates. The candidates obtained from the malaria box set were subjected to conformational scanning and geometry optimization in the Spartan'14 program to determine their physicochemical properties. According to the obtained results from the AutoDock Vina and multiple regression analyses were conducted for each protein to examine the relationship between binding affinities and the calculated physicochemical parameters of the candidates.</p><p><strong>Results: </strong>In the regression study of 200 molecules examined for 4 different proteins, 108 molecules were included for DBP, 96 for MSP1, 90 for AMA1 and 96 for RH5, and 21 common molecules were observed for all proteins.</p><p><strong>Conclusion: </strong>Twenty-one molecules showed correlation with the proteins studied. Among these molecules, MMV019074, MMV019662 and MMV665881 were suggested as candidate drug leads in terms of their binding affinities, physicochemical properties and SwissADME values.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Frequency of <i>Cryptosporidium</i> spp. in Immunosuppressed Patients with Diarrhea.","authors":"Duygu Beder, Fatma Esenkaya Taşbent","doi":"10.4274/tpd.galenos.2025.88700","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.88700","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to investigate the frequency of <i>Cryptosporidium</i> spp. in immunosuppressed patients who were admitted with diarrhea by microscopic, serological, and molecular methods and to evaluate the results in comparison with the direct fluorescent antibody (DFA) method.</p><p><strong>Methods: </strong>We analyzed 90 stool samples from immunosuppressed patients with diarrhea. All stool samples were examined using modified acid-fast staining, polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) tests for the detection of <i>Cryptosporidium</i> spp. samples examined with the DFA method were selected randomly, including samples found positive in other diagnostic tests. Stool samples that were positive in any of these diagnostic tests were evaluated by immunochromatographic card test.</p><p><strong>Results: </strong>Our study included 90 samples, of which 44 (48.8%) and 46 (51.2%) were from male and female patients, respectively. The mean age of the patients was 37.8±27.5. Thirty-one (34.4%) of the samples were from pediatric patients and their mean age was 5.3±4.34. The methods used did not show a statistically significant difference between the positivity status and patient age and gender (p>0.05). <i>Cryptosporidium</i> spp. positivity was detected in five samples (5.6%), two samples (2.2%), and one sample (1.1%) by using the modified acid-fast staining, PCR, and ELISA methods, respectively. Six of 51 samples to which the DFA method was applied were detected positive. In 4 of the 7 stool samples that were found positive by any of these methods, <i>Cryptosporidium</i> spp. was detected positive by the immunochromatographic card test.</p><p><strong>Conclusion: </strong>When the DFA method is accepted as the gold standard method in the diagnosis of <i>Cryptosporidium</i> spp., the modified acid-fast method can be used in routine diagnosis with high sensitivity and specificity. Besides, it immunochromatographic diagnostic tests thought to be useful in laboratories with intensive workflow due to their practical use and rapid results.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 1","pages":"10-16"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The First Microsatellite-based Characterisation of <i>Blastocystis</i> sp. ST3 Isolates and Population Structure Analysis.","authors":"Sema Ertuğ, Erdoğan Malatyalı, Hatice Ertabaklar, Bülent Bozdoğan, Mahmut Sinecen, Özgür Güçlü","doi":"10.4274/tpd.galenos.2025.56933","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.56933","url":null,"abstract":"<p><strong>Objective: </strong><i>Blastocystis</i> sp. is an intestinal Stramenopile that can infect both humans and animals. Genetic variability of <i>Blastocystis</i> has been investigated with a variety of molecular methods and different subtypes (ST) have been identified. The present study aimed to characterize microsatellite loci and population structure for <i>Blastocystis</i> sp. ST3, the most common ST in human faecal samples.</p><p><strong>Methods: </strong>The genome of <i>Blastocystis</i> sp. ST3 in GenBank was analyzed for the presence of microsatellites containing at least eight repeat units. Specific primers were designed for each locus and polymorphisms were identified using bioinformatics tools. The population structure was determined, and microsatellite typing was conducted on 18 <i>Blastocystis</i> sp. ST3 genomic DNA samples from the routine laboratory at Aydın Adnan Menderes University Hospital.</p><p><strong>Results: </strong>The whole-genome scan of <i>Blastocystis</i> sp. ST3 revealed 12 microsatellite loci with at least eight repeats. All loci were successfully amplified with the designed primers and eight of them were selected for genotyping. Microsatellite polymorphism analysis showed that each isolate had a unique profile (18 isolates, 18 different microsatellite types). Furthermore, the isolates were grouped into two distinct population clusters.</p><p><strong>Conclusion: </strong><i>Blastocystis</i> sp. ST3 isolates exhibited significant variability in their microsatellite repeats. The polymorphic microsatellite loci offer a novel approach to study the detailed genetic diversity and population structure of <i>Blastocystis</i> sp. ST 3.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 1","pages":"17-22"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A New Imaging Method in Dermatology: Smartphone-based Microscope.","authors":"Aslan Yürekli, İlkay Can, Melis Bal Akdoğan","doi":"10.4274/tpd.galenos.2025.24855","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.24855","url":null,"abstract":"","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 1","pages":"45-46"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144039086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Characterization of <i>Culicoides</i> Species and Their Vector Potentials for Haemosporidia Infections in the İzmir Region of Türkiye.","authors":"Hakan Yeşilöz, Zuhal Önder, Alparslan Yıldırım","doi":"10.4274/tpd.galenos.2025.32154","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.32154","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to determine the <i>Culicoides</i> species distributed across different districts of İzmir province, reveal their molecular characterization, and assess their vector potential for the transmission of avian haemosporidian parasites.</p><p><strong>Methods: </strong>The study material comprised 800 female <i>Culicoides</i> specimens collected from Bergama, Ödemiş, Kemalpaşa, and Foça districts between May and August 2016. Following morphological identification, specimens from each identified species underwent molecular analyses. The <i>mt-COI</i> gene region of genomic DNA isolates from the specimens was amplified by polymerase chain reaction (PCR) and subjected to sequence analyses to reveal their molecular characterization and phylogenetic relationships. Haemosporidian DNA was investigated by nested PCR in the gDNA isolates of head/thorax (HTP) and abdomen pools, constituted from specimens separated by species and location. Molecular characterization of identified parasites was performed using sequence analyses.</p><p><strong>Results: </strong>Morphological identification revealed that <i>C. circumscriptus</i> (39.4%) and <i>C. imicola</i> (33.8%) were the most common species in the research areas, followed by <i>Culicoides</i> sp. (ERU-Izm-Culi1) (9.1%), <i>C. nubeculosus</i> complex (7.6%), <i>C. obsoletus</i> (4.3%), <i>C. gejgelensis</i> (2.3%), <i>C. punctatus</i> (1.9%), and <i>C. newsteadi</i> (1.8%). A total of 175 polymorphic sites were distributed among the COI sequences of the obtained isolates, leading to the detection of 18 different haplotypes. The highest haplotype diversity was observed in <i>C. circumscriptus</i>, <i>C. punctatus</i>, and <i>C. newsteadi</i>. Phylogenetic analyses clustered the characterized haplotypes of <i>Culicoides</i> species into three major groups. <i>Haemoproteus</i> sp. GAGLA05 and <i>H. minutus</i> TURDUS2 lineages were detected in <i>C. circumscriptus</i> HTP genomic DNA isolates, providing evidence of this species' vector potential for <i>Haemoproteus</i> lineages in the research area.</p><p><strong>Conclusion: </strong>This study determined the <i>Culicoides</i> species distributed in the İzmir Region using an integrated morphological and molecular diagnostic approach, providing original data for the molecular epidemiology of these important flies. Furthermore, the results suggest the potential importance of <i>C. circumscriptus</i> in the transmission dynamics of <i>Haemoproteus</i> lineages.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 1","pages":"35-44"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144064688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of miR-146a Expression Levels in Archived Serum Samples for the Diagnosis/Follow-up of Patients with Cystic Echinococcosis.","authors":"Eylem Akdur Öztürk, Mesude Angın, Çağıl Coşkun, Ayşegül Ünver","doi":"10.4274/tpd.galenos.2025.66487","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.66487","url":null,"abstract":"<p><strong>Objective: </strong>Cystic echinococcosis (CE) is a zoonotic disease that causes fluid-filled cysts in internal organs and is a major public health problem worldwide. The lack of standardized methods for the diagnosis/follow-up of CE disease necessitates the development of new non-invasive diagnostic tools, such as the determination of changes in the expression levels of circulating microRNAs (miRNA). In this study, we aimed to investigate the presence of miR-146a in archived serum samples of CE patients for the first time and to evaluate its potential role in the diagnosis and follow-up of CE over a three-year period.</p><p><strong>Methods: </strong>This study included archived serum samples from 39 CE patients, 56 follow-up samples from 14 CE patients, and 3 healthy controls, and expression levels of miR-146a were evaluated in each group using quantitative real-time polymerase chain reaction. Due to the small and unbalanced control group, bootstrapped confidence intervals were used; time-dependent changes in follow-up patients were analyzed using a linear mixed-effects model and Welch's F test to address variance heterogeneity.</p><p><strong>Results: </strong>The <i>miR146</i> gene was found to be significantly upregulated in archived serum samples of patients with CE compared to healthy control samples. Additionally, the expression level of the <i>miR146</i> gene in follow-up serum samples significantly decreased in the third year post-surgery compared to follow-up blood samples taken in previous years (p<0.05).</p><p><strong>Conclusion: </strong>According to the obtained results, it was concluded that miR-146a can be recommended as a diagnostic biomarker in the diagnosis and follow-up of CE, and archived materials of CE patients can be utilized in new biomarker research.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 1","pages":"23-28"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144047101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retrospective Evaluation of <i>Toxoplasma gondii</i> Test Results in Patients Applying to A Research Hospital.","authors":"Sedef Zeliha Öner, İlknur Kaleli, Melek Demir, Ahmet Çalışkan, Ergun Mete, Hande Şenol, Çağrı Ergin","doi":"10.4274/tpd.galenos.2025.44227","DOIUrl":"10.4274/tpd.galenos.2025.44227","url":null,"abstract":"<p><strong>Objective: </strong>This study retrospectively evaluates our laboratory <i>Toxoplasma gondii</i> immunoglobulin (Ig)M, IgG antibody and avidity test results to determine the distribution of the pathogen according to sex, age, clinics and years.</p><p><strong>Methods: </strong>The serum samples sent to Pamukkale University Healthcare Research and Practice Hospital's Medical Microbiology laboratory between January 2016 and December 2023 were evaluated for <i>T. gondii</i> seropositivity. <i>Toxoplasma</i> antibodies and avidity testing were studied using chemiluminescent microparticle immunological testing method (Abbott Architect i2000SR, Weisbaden, Germany).</p><p><strong>Results: </strong>The study evaluated 42,817 tests ordered from patients with suspected toxoplasmosis. Five hundred-three (2.1%) were found to be anti-<i>T. gondii</i>-IgM positive and 4.406 (24.7%) to be anti-<i>T. gondii</i>-IgG positive. Anti-<i>T. gondii</i>-IgM and anti-<i>T. gondii</i>-IgG positivity were significantly higher in women than in men (p=0.0001; p=0.0001, respectively). Anti-<i>T. gondii</i>-IgM positivity was found to be high between the ages of 19 and 49 (p=0.0001). The anti-<i>T. gondii</i>-IgG positivity rate increased with age (p=0.0001). Anti-<i>T. gondii</i>-IgM and anti-<i>T. gondii</i>-IgG positivity rates have decreased significantly over the last four years (2020-2023) compared to the first four years (2016-2019) (p=0.0001; p=0.0001, respectively). When the results of 582 patients with <i>T. gondii</i>-avidity requests were evaluated, 38.1% of the anti-<i>T. gondii</i>-IgM positive patients and 7.8% of the anti-<i>T. gondii</i>-IgM negative patients had low avidity.</p><p><strong>Conclusion: </strong><i>T. gondii</i> seroprevalence was found to be similar to the global average, with female sex, age and nationality among the risk factors. It has also been observed that seroprevalence has significantly decreased in recent years.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 4","pages":"245-250"},"PeriodicalIF":0.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Hydatid Cyst Cases: A Single-center Retrospective Study.","authors":"Suzan Şahin, Bülent Kaya","doi":"10.4274/tpd.galenos.2024.79553","DOIUrl":"10.4274/tpd.galenos.2024.79553","url":null,"abstract":"<p><strong>Objective: </strong>Cystic echinococcosis (CE) is a zoonotic condition that can be encountered, particularly in developing countries, and leads to significant economic losses. This study was planned to observe the treatment options, complications, in the patients we followed.</p><p><strong>Methods: </strong>Patients aged 18 and over who were diagnosed with hydatid cyst and followed in our hospital between January 2018 and December 2023 were included in the study. Data were obtained from the hospital's record system. The patients with CE were retrospectively evaluated in terms of age, gender, cyst location, treatment method applied, presence of relapse, and complications.</p><p><strong>Results: </strong>A total of 30 patients, with a mean age of 42.8 years (range: 19-68), were included in the study; 13 (43.3%) were male and 17 (56.7%) were female. The most common presenting complaint was abdominal pain (n=14, 46.7%), and 6 patients (20.0%) were asymptomatic. Sixteen patients had multiple cysts in the same region, and 6 patients had cysts in different regions. The most common site of involvement was the liver (n=21, 70.0%), followed by the lungs in 4 patients (13.3%). Single cases of brain, spinal cord, spleen, kidney, and bone involvement were observed. Diagnosis was made by ultrasonography in 16 patients (53.3%), magnetic resonance imaging in 8 patients (26.7%), and computed tomography in 6 patients (20.0%). Surgical intervention was performed in 20 patients (66.7%), and percutaneous drainage in 3 patients (10.0%). All patients received albendazole treatment. Complications included intra-abdominal abscess in three patients (10.0%) and rupture in one patient. One patient with intracranial involvement died.</p><p><strong>Conclusion: </strong>Although observed worldwide, CE maintain their importance in terms of morbidity and mortality, particularly in developing countries.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 4","pages":"222-227"},"PeriodicalIF":0.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Parasite That Should not be Neglected in Patients with Ulcerative Colitis: <i>Entamoeba histolytica</i>.","authors":"Murat Soylu, Abdurrahman Ekici, Selahattin Aydemir, Şehriban Yürektürk, Önder Akkaş","doi":"10.4274/tpd.galenos.2025.81894","DOIUrl":"10.4274/tpd.galenos.2025.81894","url":null,"abstract":"<p><strong>Objective: </strong>To determine the prevalence of amoebiasis, which has been neglected in recent years according to the World Health Organization, in ulcerative colitis patients and investigate the relationship between amoebiasis and ulcerative colitis.</p><p><strong>Methods: </strong>The study included 150 individuals, including 100 ulcerative colitis patients and 50 healthy individuals without gastrointestinal complaints. The samples collected were first analyzed macroscopically and then using native-Lugol, trichrome staining, and enzyme-linked immunosorbent assay (ELISA).</p><p><strong>Results: </strong>In the microscopic examination, <i>Entamoeba</i> spp. cysts were found in 22% of the ulcerative colitis patients and 2% of those in the control group. <i>Entamoeba histolytica</i> (<i>E. histolytica</i>) adhesin antigen was detected by ELISA in 31% of the ulcerative colitis patients and 4% of those in the control group. A significant correlation was found between the incidence of <i>E. histolytica</i> and ulcerative colitis in the statistical evaluation.</p><p><strong>Conclusion: </strong><i>E. histolytica</i> should not be neglected in ulcerative colitis patients and should be investigated in the presence of diarrhea, bloody diarrhea, and abdominal pain.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"48 4","pages":"251-255"},"PeriodicalIF":0.0,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143025145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}