Turkiye parazitolojii dergisi最新文献

{"title":"<i>Fasciola hepatica</i> Diagnosis: Clinical and Laboratory Clues from A University Hospital Experience.","authors":"Ayşe Sağmak Tartar, Mehmet Ali Aşan, Ahmet Bozdağ, İbrahim Halil Bahçecioğlu, Ayhan Akbulut, Kutbeddin Demirdağ","doi":"10.4274/tpd.galenos.2025.42650","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.42650","url":null,"abstract":"<p><strong>Objective: </strong>Fascioliasis is a trematode infection caused by <i>Fasciola hepatica</i> or <i>Fasciola gigantica</i>. Diagnosis of fascioliasis is often delayed. This study aims to contribute to reducing the incidence of the disease by determining regional epidemiology and guiding public health measures, as well as increasing awareness among physicians through the examination of clinical, laboratory, and imaging findings.</p><p><strong>Methods: </strong>Patients diagnosed with fascioliasis at the Infectious Diseases Clinic and Gastroenterology Clinic of Fırat University Hospital between 2011 and 2022 were included in the study. Demographic information, clinical findings, complete blood count, biochemical parameters, radiological imaging reports, treatment, and prognosis were examined. Patient data were obtained from the hospital automation system, files, and epicrisis.</p><p><strong>Results: </strong>Of the 19 patients followed, 15 (78.9%) were female. The mean age was 62.36±12.30 years. Fifteen patients (78.9%) lived in rural areas. Seven patients (36.8%) were involved in animal husbandry. Twelve patients (63.2%) had a history of consuming watercress. The most commonly observed symptoms were loss of appetite, right upper quadrant pain, nausea-vomiting, and night sweats. All patients were treated with triclabendazole without any side effects. Statistically significant differences were found in the levels of eosinophilia, alanine transaminase, gamma-glutamyl transferase, alkaline phosphatase, total bilirubin, and direct bilirubin between the beginning of treatment and 1 month in our patients (p<0.05). One patient was lost due to intervening cardiac problems.</p><p><strong>Conclusion: </strong>Fascioliasis is also present in our region, and a significant history of watercress consumption is noteworthy epidemiologically. <i>Fasciola hepatica</i> should be considered in patients presenting with abdominal pain accompanied by peripheral eosinophilia. Increasing awareness among physicians will contribute to preventing delays in diagnosis and treatment.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 2","pages":"58-62"},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency of Intestinal Parasites in Patients Admitted to the Microbiology Laboratory of Siirt Training and Research Hospital.","authors":"Leyla Gündüz, Selahattin Aydemir, Zeynep Taş Cengiz, Hasan Yılmaz","doi":"10.4274/tpd.galenos.2025.97659","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.97659","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to determine the frequency of intestinal parasites in patients admitted to the Microbiology Laboratory of Siirt Training and Research Hospital.</p><p><strong>Methods: </strong>The study was conducted between 19.04.2021 and 30.11.2021 with a total of 300 patients (150 children and 150 adults) between the ages of 1-90 years who were referred to the Microbiology Laboratory with a request for stool sample analysis and who admitted to Siirt Training and Research Hospital with different complaints. The samples were evaluated by nativ-Lugol, formol-ethyl acetate concentration and modified acid-fast staining methods. The samples in which <i>Entamoeba</i> spp. eggs were detected were evaluated for <i>Entamoeba histolytica/Entamoeba dispar</i> antigen using <i>Entamoeba</i> antigen cassette test.</p><p><strong>Results: </strong>In this study, one or more than one type of intestinal parasite was found in 21.3% of 150 pediatric patients, 24% of 150 adult patients and 22.7% of 300 patients. The highest rate was <i>Blastocystis</i> (18%) and the lowest rate was <i>Ascaris lumbricoides</i> (0.7%). Although intestinal parasites were found at a higher rate in adults (24%) compared to pediatric age group (21.3%), there was no significant difference between the age groups in terms of parasite frequency in the statistical evaluation. There was a statistically significant difference between diarrhea (p=0.022) and anorexia (p=0.014) and intestinal parasite positivity.</p><p><strong>Conclusion: </strong>It was concluded that it would be appropriate to evaluate patients admitted to hospitals with complaints such as diarrhea and loss of appetite in terms of intestinal parasites. Although this study gives an idea about the prevalence of intestinal parasites in Siirt Region, there is a need for larger scale studies in the region including more people.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 2","pages":"63-67"},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bibliometric Analysis of All Theses on Echinococcosis in Türkiye.","authors":"İsmail Davarcı, Hüseyin Güdücüoğlu","doi":"10.4274/tpd.galenos.2025.09226","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.09226","url":null,"abstract":"<p><strong>Objective: </strong>Türkiye is the country with the highest number of published articles on echinococcosis worldwide, it is expected that medical specialization, PhD and master's students in Türkiye would conduct theses on this topic. These theses can provide insights for future studies. Therefore, this study aims to evaluate the medical specialization, PhD, and master's theses related to echinococcosis conducted in Türkiye.</p><p><strong>Methods: </strong>The relevant theses were accessed individually from the \"detailed search\" section of the National Thesis Center webpage of the Council of Higher Education by using the search terms \"Ekinokok\", \"<i>Echinococcus</i>\", or \"Hidatik\" without selecting a year range.</p><p><strong>Results: </strong>A total of 202 theses (113 medical specialization theses, 42 PhD theses, and 47 master's theses) were included in the study. When examined on a provincial basis, it was found that the highest number of theses were conducted in Ankara (n=36). At the university level, İstanbul University had the most theses. Medical specialization theses were mostly conducted in the fields of general surgery and radiology, while PhD and master's theses were primarily conducted in the fields of parasitology and veterinary medicine. Most of the theses conducted in the internal and surgical sciences were retrospective in design, while those in the basic sciences were mainly diagnostic in nature. A moderate positive correlation was found between the number of theses and the years for medical theses (r=0.341, p=0.027), and a strong positive correlation was found for PhD theses (r=0.505, p=0.001), master's theses (r=0.619, p=0.000), and all theses combined (r=0.761, p=0.000).</p><p><strong>Conclusion: </strong>It was observed that at least one thesis related to echinococcosis is conducted each year, with an increase in the number of PhD and master's theses in recent years.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 2","pages":"74-81"},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging Insights into Feline Hepatozoonosis in Türkiye: Molecular Detection and Phylogenetic Characterization from the Aegean Coast.","authors":"Selin Hacılarlıoğlu, Metin Pekağırbaş","doi":"10.4274/tpd.galenos.2025.88609","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.88609","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study is to investigate the presence, prevalence, and molecular characteristics of <i>Hepatozoon</i> spp. infection in domestic cats living in Aydın and İzmir provinces, located on the Aegean coast of Türkiye. The study specifically focuses on species identification and genotype distribution.</p><p><strong>Methods: </strong>A total of 203 blood samples from domestic cats were analyzed for <i>Hepatozoon</i> spp. using polymerase chain reaction (PCR). Among the 67 PCR-positive samples, 10 (five for each province) were selected for sequencing, and bidirectional Sanger sequencing was performed. The obtained sequences were evaluated using basic local alignment search tool and phylogenetic analyses, and genotype identification was based on the 18S rRNA gene region. Additionally, potential risk factors such as age, sex, health status and housing type were statistically analyzed for their association with <i>Hepatozoon</i> spp. infection.</p><p><strong>Results: </strong>The overall prevalence of <i>Hepatozoon</i> spp. in cats was determined to be 33.0% (67/203). The infection rate was 43.69% in Aydın and 22% in İzmir, and this difference was found to be statistically significant (p=0.001). All 10 sequenced samples were identified as <i>Hepatozoon felis</i>, and all isolates belonged to Genotype I. A significant association was found between age and infection, with a particularly high infection rate of 44% observed in cats aged one year or younger (p=0.020).</p><p><strong>Conclusion: </strong>This study revealed a high prevalence of <i>Hepatozoon</i> spp. infection among cats in western Türkiye, with all sequenced isolates identified as Genotype I. The notably high infection rate observed in young cats raises the possibility of transplacental transmission. These findings underscore the need for further investigations to clarify the transmission routes and risk factors associated with this protozoan parasite, the epidemiology of which remains insufficiently understood.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 2","pages":"52-57"},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of <i>Lucilia sericata</i> Larval Secretion on <i>Echinococcus granulosus</i>.","authors":"Feza İrem Aldı, Kıymet Tabakçıoglu, Erdal Polat, Nermin Şakru","doi":"10.4274/tpd.galenos.2025.36854","DOIUrl":"10.4274/tpd.galenos.2025.36854","url":null,"abstract":"<p><strong>Objective: </strong><i>Echinococcus granulosus</i> causes echinococcosis when its larvae settle in various organs, especially the liver and lung, of humans and herbivorous animals such as sheep and cattle. Echinococcosis are endemic in Mediterranean countries including Türkiye, the Middle East and South Africa. Echinococcosis treatments are generally surgical excision or benzimidazoles. Maggot debridement therapy is a wound treatment with <i>Lucilia sericata</i> larvae. While the larvae consume the old-damaged tissues and bacteria in the wound site, the maggots secrete an antimicrobial fluid. In this study, we aimed to determine the effect of <i>Lucilia sericata</i> larval secretions on <i>Echinococcus granulosus</i> protoscoleces <i>in vitro</i>.</p><p><strong>Methods: </strong>Various DNA damage markers were used to analyze the therapeutic potential of the larval secretions. For this purpose, protoscoleces were cultured and treated with different concentrations of larval secretions. Comet test was performed to determine DNA damage. Expression of <i>EgATM, EgRad9</i> and <i>EgTopo2a</i> genes was analyzed by quantitative real time polymerase chain reaction.</p><p><strong>Results: </strong>The viability of the control group was 94% and the viability of the protoscoleces treated with larval secretions was 73%. Comet test showed that larval secretions caused DNA damage in protoscoleces. According to quantitative real time polymerase chain reaction results; 1:1 larval secretions increased <i>ATM</i> and <i>Rad9</i> gene expression 3.2-fold and <i>Topo2a</i> gene expression 2.2-fold compared to control groups (p<0.05).</p><p><strong>Conclusion: </strong>These data showed that in vitro larval secretion induced DNA damage in <i>Echinococcus granulosus</i> protoscoleces and increased the expression of <i>EgATM, EgRad9</i> and <i>EgTopo2a</i> genes. However, further <i>in vitro</i> and <i>in vivo</i> studies are needed.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 2","pages":"82-87"},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of <i>Demodex</i> Prevalence Due to Mask Use After the COVID-19 Pandemic with Cellophane Tape Method.","authors":"Ahmet Duran Ataş, Berna Baysal Bakay","doi":"10.4274/tpd.galenos.2025.43265","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.43265","url":null,"abstract":"<p><strong>Objective: </strong><i>Demodex</i> species in the family Demodicidae are hair follicle scabies agents. <i>Demodex</i> species are transmitted from person to person through close contact, shared towels, make-up materials, etc. This study was conducted to obtain data on the relationship between mandatory mask use and demodicosis during the Coronavirus disease-2019 (COVID-19) period.</p><p><strong>Methods: </strong>The study included 510 students who used masks in necessary environments since the beginning of the pandemic and participated in the study voluntarily. Cellophane tapes were applied to the relevant areas three times. In addition, both eye lashes were pulled from both eyes and adhered to the cellophane tapes. The samples were examined under light microscope at different magnifications (x10, x40).</p><p><strong>Results: </strong><i>Demodex folliculorum</i> and <i>Demodex</i> brevis were detected in 38 (7.5%) of 510 students. <i>D. folliculorum</i> was detected in 33 of the positive students and both <i>D. folliculorum</i> and <i>D. brevis</i> were detected in 5 students, 2 of whom were male and 3 of whom were female. <i>Demodex</i> spp. was found in 12 (4.9%) of 245 students who answered \"no\" to the question \"Do you have acne or skin complaints on your face?\" and in 26 (9.8%) of 265 students who answered \"yes\". While this parameter was statistically significant, the other parameters were not statistically significant.</p><p><strong>Conclusion: </strong>The COVID-19 period has changed people's lifestyles and habits in many ways. It has made the use of masks obligatory. During mandatory mask use, factors that may increase the presence of <i>Demodex</i> spp. should not be ignored.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 2","pages":"68-73"},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic Diversity Analysis of <i>Cysteine Proteinase B</i> Gene in <i>Leishmania major</i> Isolates from Southwest Iran.","authors":"Mahmoud Rahdar, Mohammad Reza Maghsoudi, Molouk Beiromvand, Tahereh Kardooni","doi":"10.4274/tpd.galenos.2025.92400","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.92400","url":null,"abstract":"<p><strong>Objective: </strong>Selecting an effective target for <i>Leishmania</i> vaccination requires identifying a protein antigen with low or no genetic diversity. The <i>cysteine proteinase B</i> (CPB) gene has emerged as a promising immunogenic target, though its diversity requires evaluation across different regions. Given Iran's status as a hyperendemic region for cutaneous leishmaniasis, this study aimed to collect and analyze 30 <i>Leishmania major</i> isolates from various areas of Khuzestan Province in Southwestern Iran.</p><p><strong>Methods: </strong>The <i>CPB</i> gene was amplified via polymerase chain reaction, and its nucleotide sequence was determined. Sequence analysis was performed using MEGA5 software, with subsequent comparison to National Center for Biotechnology Information database entries. A phylogenetic tree was constructed to compare isolated strains with reference strains from other geographic regions and species.</p><p><strong>Results: </strong>The phylogenetic analysis demonstrated that the <i>CPB</i> gene of the isolated strains in different regions of Khuzestan province formed a clade with certain <i>Leishmania major</i> strains from various parts of the world. Overall, the genotypic analysis of the <i>CPB</i> gene in Khuzestan province indicated genetic similarity among 29 isolates. In contrast, one sample from Dezful (north of Khuzestan Province) exhibited a significant difference from the reference strain, resulting in notable amino acid changes.</p><p><strong>Conclusion: </strong>Based on these findings, the <i>CPB</i> gene holds promise as a potential candidate for vaccination development against cutaneous leishmaniasis in this region.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 2","pages":"47-51"},"PeriodicalIF":0.0,"publicationDate":"2025-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144660554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical Features and Quality of Life in Patients with Scabies.","authors":"Münevver Güven, Aybüke Parlakdağ Kılcıoğlu","doi":"10.4274/tpd.galenos.2025.70894","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.70894","url":null,"abstract":"<p><strong>Objective: </strong>Scabies is a contagious cutaneous infestation characterized by intense itching. In this study, we aimed to evaluate the clinical features of scabies and the effect of scabies on quality of life, and the change in quality of life with treatment.</p><p><strong>Methods: </strong>This prospective and cross-sectional study included 40 adults patients with classical scabies. Detailed skin examinations of the patients were performed. Dermatological life quality index (DLQI) was used to evaluate quality of life in patients with scabies. Visual analog scale (VAS) was used to investigate the severity of nocturnal itching and itch-related sleep disturbance. VAS levels and DLQI scores of the patients were evaluated at baseline (week 0) and after treatment (weeks 2 and 4).</p><p><strong>Results: </strong>The most common lesion in patients was tunnel (100%), followed by papule (97.5%), crusted papule (85%) and excoriation (77.5%). The abdomen (90%) was the most common area of lesions, followed by hands (87.5%) and forearm (85%). It was determined that the quality of life of 75% of the patients was moderate to extremely large affected. There was a significant improvement in average DLQI scores of patients after treatment compared to before treatment (p<0.001, week 0: 11.50±7.81, week 2: 5.50±4.68, week 4: 1.05±2.08). The baseline VAS values of the patients for nocturnal itching and itch-related sleep disturbance were 7.22±2.80 and 6.30±3.60, respectively. A statistically significant improvement was detected in these values in the week 2 and week 4 (p<0.001). Thirty-nine of the patients were given 10% sulfur ointment (3 consecutive days) and 35 of these patients (89.7%) recovered.</p><p><strong>Conclusion: </strong>Scabies has significant impact on quality of life in the vast majority of patients. Itch-related sleep disturbance is common in patients with scabies. Sulfur is an effective treatment choice for scabies.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 1","pages":"29-34"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143989463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of New Candidate Inhibitors Able to Prevent Erythrocyte Invasion in Malaria by Drug Screening.","authors":"Ahmet Burak Doğanoğlu, Vildan Enisoğlu Atalay","doi":"10.4274/tpd.galenos.2025.37928","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.37928","url":null,"abstract":"<p><strong>Objective: </strong>In Nowadays malaria still remains the parasitic disease causing the highest number of deaths, accounting for 619,000 fatalities. The <i>Plasmodium</i> parasites that cause malaria have separate life cycles in both humans and female Anopheles mosquitoes, existing in various forms throughout this process. The main reason for observing the disease is that merozoites sustain their existence by invading erythrocytes. Existing drugs affect the parasite's ability to digest hemoglobin. Drug resistance is also involved in this process. In this study, have been focused to develop new drug candidate molecules for evade drug resistance. To evade drug resistance, the aim was to prevent merozoites from invading erythrocytes.</p><p><strong>Methods: </strong>The invasion of merozoites into erythrocytes consists of several stages: Attachment, deformation, apical junction formation, and tight junction formation. For this purpose, the docking calculations have been done between the invasion proteins such as MSP1, pvDBP, phRH5, AMA1 and candidates. The candidates obtained from the malaria box set were subjected to conformational scanning and geometry optimization in the Spartan'14 program to determine their physicochemical properties. According to the obtained results from the AutoDock Vina and multiple regression analyses were conducted for each protein to examine the relationship between binding affinities and the calculated physicochemical parameters of the candidates.</p><p><strong>Results: </strong>In the regression study of 200 molecules examined for 4 different proteins, 108 molecules were included for DBP, 96 for MSP1, 90 for AMA1 and 96 for RH5, and 21 common molecules were observed for all proteins.</p><p><strong>Conclusion: </strong>Twenty-one molecules showed correlation with the proteins studied. Among these molecules, MMV019074, MMV019662 and MMV665881 were suggested as candidate drug leads in terms of their binding affinities, physicochemical properties and SwissADME values.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 1","pages":"1-9"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144062559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of the Frequency of <i>Cryptosporidium</i> spp. in Immunosuppressed Patients with Diarrhea.","authors":"Duygu Beder, Fatma Esenkaya Taşbent","doi":"10.4274/tpd.galenos.2025.88700","DOIUrl":"https://doi.org/10.4274/tpd.galenos.2025.88700","url":null,"abstract":"<p><strong>Objective: </strong>We aimed to investigate the frequency of <i>Cryptosporidium</i> spp. in immunosuppressed patients who were admitted with diarrhea by microscopic, serological, and molecular methods and to evaluate the results in comparison with the direct fluorescent antibody (DFA) method.</p><p><strong>Methods: </strong>We analyzed 90 stool samples from immunosuppressed patients with diarrhea. All stool samples were examined using modified acid-fast staining, polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) tests for the detection of <i>Cryptosporidium</i> spp. samples examined with the DFA method were selected randomly, including samples found positive in other diagnostic tests. Stool samples that were positive in any of these diagnostic tests were evaluated by immunochromatographic card test.</p><p><strong>Results: </strong>Our study included 90 samples, of which 44 (48.8%) and 46 (51.2%) were from male and female patients, respectively. The mean age of the patients was 37.8±27.5. Thirty-one (34.4%) of the samples were from pediatric patients and their mean age was 5.3±4.34. The methods used did not show a statistically significant difference between the positivity status and patient age and gender (p>0.05). <i>Cryptosporidium</i> spp. positivity was detected in five samples (5.6%), two samples (2.2%), and one sample (1.1%) by using the modified acid-fast staining, PCR, and ELISA methods, respectively. Six of 51 samples to which the DFA method was applied were detected positive. In 4 of the 7 stool samples that were found positive by any of these methods, <i>Cryptosporidium</i> spp. was detected positive by the immunochromatographic card test.</p><p><strong>Conclusion: </strong>When the DFA method is accepted as the gold standard method in the diagnosis of <i>Cryptosporidium</i> spp., the modified acid-fast method can be used in routine diagnosis with high sensitivity and specificity. Besides, it immunochromatographic diagnostic tests thought to be useful in laboratories with intensive workflow due to their practical use and rapid results.</p>","PeriodicalId":34974,"journal":{"name":"Turkiye parazitolojii dergisi","volume":"49 1","pages":"10-16"},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144001255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}