Journal of Chromatography B最新文献

{"title":"An ID-HPLC-MS/MS based candidate reference measurement procedure for the quantification of imipenem, meropenem and ertapenem in human plasma","authors":"Jing Lin ,&nbsp;Qingqing Pan ,&nbsp;Weihua Wang ,&nbsp;Shuangshuang Chen ,&nbsp;Guangliang Wu ,&nbsp;Min Shen ,&nbsp;Huoyan Ji","doi":"10.1016/j.jchromb.2025.124908","DOIUrl":"10.1016/j.jchromb.2025.124908","url":null,"abstract":"<div><h3>Background</h3><div>Carbapenems require precise plasma levels for optimal therapy, yet no LC-MS/MS method offered SI-traceability or reference-grade performance. In this study, a candidate reference measurement procedure (RMP) using isotope dilution high-performance liquid chromatography-tandem mass spectrometry (ID-HPLC-MS/MS) was developed and validated for accurate quantification of imipenem (IPM), meropenem (MEM) and ertapenem (ETP) in human plasma.</div></div><div><h3>Methods</h3><div>To ensure traceability to SI units, the absolute content of the IPM, MEM and ETP primary reference materials (PRMs) were determined by quantitative nuclear magnetic resonance (qNMR) spectroscopy. Samples were prepared by protein precipitation followed by dilution. Method validation was performed according to guidances from the Clinical and Laboratory Standards Institute (CLSI), including selectivity/specificity, matrix effect, carryover, linearity, limit of quantification (LOQ) and limit of detection (LOD), trueness and precision, and stability. Measurement uncertainty was evaluated according to the Guide to the Expression of Uncertainty in Measurement (GUM). Moreover, the candidate RMP was compared between two independent laboratories.</div></div><div><h3>Results</h3><div>The method showed high selectivity and stability. Linear ranges were 0.208 to 52.087 μg/mL (IPM, <em>r</em> = 0.9997), 0.412 to 103.427 μg/mL (MEM, <em>r</em> = 0.9999) and 0.083 to 20.791 μg/mL (ETP, <em>r</em> = 0.9999). Mean recoveries were 100.05 to 104.80 %, 92.34 to 94.77 % and 95.55 to 100.04 %, respectively. The method demonstrated high sensitivity, with limits of LOQ of 0.103, 0.103 and 0.021 μg/mL and corresponding LOD of 0.0077, 0.0018 and 0.0006 μg/mL for IPM, MEM and ETP, respectively. No obvious matrix effect and carryover were observed. The expanded uncertainties (<em>k</em> = 2) were ≤ 6.12 %.</div></div><div><h3>Conclusion</h3><div>The qNMR-based candidate RMP provides SI-traceable, accurate simultaneous quantification of IPM, MEM and ETP, enabling standardization of clinical assays and reliable patient-sample measurement.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1270 ","pages":"Article 124908"},"PeriodicalIF":2.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145867136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lincomycin residues in liver, bovine muscle tissue, milk, poultry meat, eggs, and honey: Method development and validation","authors":"Omar Khaled ,&nbsp;Lamia Ryad ,&nbsp;Mostafa Nagi ,&nbsp;Fawzy Eissa","doi":"10.1016/j.jchromb.2025.124902","DOIUrl":"10.1016/j.jchromb.2025.124902","url":null,"abstract":"<div><div>This study presents and validates a simple, sensitive, and specific method for the determination of lincomycin in liver, bovine muscle tissue, milk, poultry meat, eggs, and honey. The method employs a liquid-liquid extraction sample preparation technique, eliminating the need for solid-phase extraction cartridges. This approach offers a cost-effective and rapid alternative, which is essential for routine analysis. The validation procedure encompassed selectivity, linearity, limit of detection, limit of quantification, recovery, repeatability, reproducibility, and decision limit. The method demonstrated excellent linearity with correlation coefficients ranging from 0.9977 to 0.9999 across all matrices. Limit of detection values ranged from 0.04 to 2 μg/kg, while limit of quantification values ranged from 0.13 to 6.6 μg/kg. Recovery rates were between 70 % and 124 % and decision limits ranged from 0.15 to 543 μg/kg. Out of the 180 real samples analyzed, 31 were detected as positive. Continuous monitoring studies should be conducted regularly to determine the presence of veterinary drug residues in food of animal origin, poultry meat, and honey. These studies will help identify the sources of these residues and facilitate the implementation of secure preventive and remedial strategies.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1270 ","pages":"Article 124902"},"PeriodicalIF":2.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145812582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of serum concentrations of α-pinene, β-pinene, and limonene in the U.S. population from the 2013–2014 National Health and nutrition examination survey","authors":"Lalith Silva,&nbsp;Cody Newman,&nbsp;Deepak Bhandari,&nbsp;Wanzhe Zhu,&nbsp;Benjamin Blount","doi":"10.1016/j.jchromb.2025.124873","DOIUrl":"10.1016/j.jchromb.2025.124873","url":null,"abstract":"<div><div>Terpenes are a class of ubiquitous chemicals with many potential health benefits and harms. Human exposure to terpenes can occur through pathways such as cleaning products, fragrances, diet, or drug-use. α-pinene, β-pinene, and limonene are known to be present in trees, citrus fruits, herbs, marijuana, and cigarette smoke. With little known about human exposure to these compounds, it was important to characterize the baseline level of exposure for the general U.S. population. Using results for 2213 samples for participants 6–80 years with surplus serum available from NHANES 2013–2014, obtained with previously published quantitation methods, we compared serum terpene concentrations in demographic groups and identified likely exposure routes. Multiple linear regression found smoking 10–19 cigarettes per day (CPD) was associated with 35.8 % higher serum limonene and smoking &gt;19 CPD was associated with 44.9 % higher serum limonene compared with non-smokers with no secondhand smoke exposure (<em>p</em> = 0.0002). In addition, we found that recent consumption of citrus-rich foods (rates per 1 kg consumed) was associated with significantly higher serum α-pinene (32.8 %, <em>p</em> = 0.0042) and limonene (82.1 % <em>p</em> = 0.0002). Finally, we observed significant associations between serum terpenes and demographic groups. Females had significantly lower serum limonene (<em>p</em> = 0.0243) compared to males. Hispanic and non-Hispanic Asian people had significantly higher serum β-pinene (<em>p</em> &lt; 0.05) and limonene (p &lt; 0.05) compared to non-Hispanic White people. This work characterizes the baseline of terpene exposure in the general U.S. population.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1270 ","pages":"Article 124873"},"PeriodicalIF":2.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145716802","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new organic hydrophilic monolith stationary phase for HILIC - ELSD topiramate quantification and separation with its main polar impurities in infusion solutions","authors":"Claire Andre ,&nbsp;Yves Claude Guillaume","doi":"10.1016/j.jchromb.2025.124901","DOIUrl":"10.1016/j.jchromb.2025.124901","url":null,"abstract":"<div><div>In this study, a hydrophilic monolith doped with fumed silica nanoparticles was in-situ prepared in a liquid chromatography column. It was clearly demonstrated the addition of silica nanoparticles in the polymerization mixture improved the formation of mesopores in the low size domain (5.2 nm) and thus the specific surface area and hydrophilicity of the monolith. Stability studies showed this monolith was not affected after slightly swelling or shrinking under different solvent conditions. The potential of this new column was exemplified by its capability in hydrophilic interaction liquid chromatography conditions (1) to separate five standard compounds (nucleotides) and (2) to monitor the main degradation products of topiramate an anticonvulsant molecule used primarily to treat epilepsy. For this second separation, the optimum HPLC gradient conditions were determined with only 18 experiments using a computer assisted design of experiments - simplex algorithm software developed in our laboratory. This column was then used successfully for the quantification of the different doses (in mg) of topiramate in a meglumin pharmaceutical formulation. This novel HPLC analytical shows promise as a stability-indicating assay and may be suitable for the quality control of topiramate in pharmaceutical préparations.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1270 ","pages":"Article 124901"},"PeriodicalIF":2.8,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145788164","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pesticide residues in aquaculture salmon filet and feed: Quantitative and qualitative screening profiles of legacy and current-use pesticides using GC-EI-MS/MS and LC-ESI-IMS-QToF","authors":"Carey E. Donald,&nbsp;Annette Bernhard,&nbsp;Marianne F. Hauglid ,&nbsp;Annie Fürstenberg,&nbsp;Marc H.G. Berntssen,&nbsp;Anne-Katrine Lundebye,&nbsp;Veronika Sele","doi":"10.1016/j.jchromb.2025.124864","DOIUrl":"10.1016/j.jchromb.2025.124864","url":null,"abstract":"<div><div>In recent decades, the ingredients used in aquaculture feeds have changed from marine-based to more plant-based. As a result, the content of legacy, organochlorine pesticides has decreased, but the potential increase of current use, land-based pesticides has not been documented. In this work, two analytical techniques were developed and applied to characterize the pesticide profile in current Norwegian salmon aquaculture after the shift to more plant-based feed ingredients. Samples of salmon filet, feed, and feed ingredients were analyzed using two different chromatographic methods, a quantitative gas-chromatography-triple quadrupole mass spectrometry method (43 analytes) and a broader, presence/absence screen using liquid chromatography-high resolution mass spectrometry (215 analytes). Among all samples and between both methods, a total of 43 different pesticide analytes were identified. Results show that the major pesticides present in the filet of farm-raised salmon are the legacy, organochlorine pesticides as contaminants. Conversely the feed is dominated by current-use, organophosphate and pyrethroid pesticides. There were no exceedances of the few established maximum residue limits for contaminants in fish and feed. No pesticides in salmon filet exceeded the default limit of 10 μg/kg, while there were several observations in feed above this level. These results suggest low risk in terms of food safety, however, issues related to animal health cannot be ruled out. The two chromatography methods are compared, and they complement each other by giving an overview of pesticides with both breadth and depth.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1269 ","pages":"Article 124864"},"PeriodicalIF":2.8,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145679492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From chemical profiling to bioactivity: Integrating spectrum-effect relationship and activity validation to discover anti-inflammatory markers in Wuwei Qingzhuo Pill","authors":"Tiantian Shi ,&nbsp;Wenhua Yang ,&nbsp;Lizhu Yin ,&nbsp;Xuena Zhang ,&nbsp;Yuewu Wang ,&nbsp;Liang Bao ,&nbsp;Ren Bu ,&nbsp;Jingkun Lu ,&nbsp;Tuya Bai ,&nbsp;Peifeng Xue ,&nbsp;Xin Dong","doi":"10.1016/j.jchromb.2025.124876","DOIUrl":"10.1016/j.jchromb.2025.124876","url":null,"abstract":"<div><div>Efficacy-oriented quality control represents a persistent challenge in traditional medicine due to complex multi-component compositions and the inadequacy of single-compound quantification methods. This study establishes an integrated analytical paradigm combining in vivo pharmacological validation with comprehensive chemical profiling, using the traditional Mongolian formulation Wuwei Qingzhuo Pill (WQP) as an investigative model. We initially demonstrated WQP's significant anti-arthritic efficacy in a collagen-induced arthritis (CIA) rat model, observing marked reduction in joint swelling, arthritis scores, and bone destruction. Subsequent chemical characterization established validated HPLC fingerprints across 19 production batches, revealing consistent quality patterns. Through innovative spectrum-effect relationship analysis integrating gray relational analysis (GRA) and orthogonal partial least squares-discriminant analysis (OPLS-DA), we successfully correlated chromatographic features with anti-inflammatory potency, identifying gallic acid, punicalagin, and ellagic acid as critical quality markers. The bioactivity of these markers was further confirmed in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages, which significantly inhibited TNF-α and IL-1β release. This research presents the first comprehensive application of HPLC fingerprinting combined with spectrum-effect relationship analysis for quality assessment of WQP, offering a robust methodology that transcends traditional quality control approaches. The identified quality markers provide a scientific foundation for efficacy-based quality control and standardization of WQP, with potential implications for quality evaluation of other traditional medicines.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1269 ","pages":"Article 124876"},"PeriodicalIF":2.8,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145663524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a UPLC-MS/MS assay for serum choline and phosphocholine with a pilot application to obesity","authors":"Yingting Wu ,&nbsp;Jiaqi Xin ,&nbsp;Haiyan Cao ,&nbsp;Yongsheng Zhao ,&nbsp;Mengxi Jiang","doi":"10.1016/j.jchromb.2025.124870","DOIUrl":"10.1016/j.jchromb.2025.124870","url":null,"abstract":"<div><div>The rising global burden of obesity underscores the need to better characterize metabolic alterations associated with this condition. Choline (Cho) and its derivative phosphocholine (ChoP) are key intermediates in phospholipid metabolism, yet their relationships with obesity remain unclear. In this study, we established and validated a UPLC-MS/MS method for quantifying serum Cho and ChoP, and investigated their associations with obesity and fasting glucose in a pilot human cohort. Serum samples from 62 participants (obese vs. nonobese) were analyzed. The primary analysis compared metabolite levels between groups, and key secondary analyses included Pearson correlations with fasting glucose and receiver operating characteristic (ROC)-based discrimination; two-sample Mendelian randomization (MR) was conducted using external GWAS summary statistics. Obese individuals had lower ChoP levels and ChoP/Cho ratios. ChoP and the ChoP/Cho ratio were inversely associated, and Cho was positively associated, with fasting glucose. ChoP showed modest discriminative ability for obesity, and MR suggested that higher ChoP levels may be related to a lower risk of obesity. Overall, we validated a UPLC-MS/MS assay for serum Cho and ChoP, and our findings indicate that ChoP and the ChoP/Cho ratio are linked to obesity and glucose status.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1269 ","pages":"Article 124870"},"PeriodicalIF":2.8,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145688901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A method for simultaneous determination of triiodothyronine and thyroxine in fingernail clippings by LC/ESI-MS/MS","authors":"Kenjiro Shibata ,&nbsp;Sakurako Tanaka ,&nbsp;Shuhei Baba ,&nbsp;Arina Miyoshi ,&nbsp;Norio Wada ,&nbsp;Tatsuya Higashi","doi":"10.1016/j.jchromb.2025.124869","DOIUrl":"10.1016/j.jchromb.2025.124869","url":null,"abstract":"<div><div>A fingernail clipping is a promising specimen for evaluating the medium-to-long term status of low-molecular weight hormones. However, the nail concentrations of thyroid hormones and their clinical significance remain poorly understood. In this study, a liquid chromatography/electrospray ionization-tandem mass spectrometry (LC/ESI-MS/MS) method was developed and validated for simultaneously quantifying triiodothyronine (T₃) and thyroxine (T₄) in the thumbnail clippings. The nail T₃ and T₄ were extracted with 50 % ethanol and purified using a Strata-X cartridge, then injected into the LC/ESI-MS/MS. ¹³C₆-T₄ was used as the internal standard for both T₃ and T₄. Validation results demonstrated that the method was precise (relative standard deviations in five repetitive measurements were ≤ 4.5 %) and accurate (analytical recovery rates were 98.3–103.3 %). The lower limits of quantification were 0.20 ng/g for both thyroid hormones. By using this method, we found that the nail concentrations of T₃ (0.74 ± 0.26 ng/g, mean ± standard deviation) were at the same level as those of T₄ (0.90 ± 0.27 ng/g) in euthyroid subjects (<em>n</em> = 40). This result might be caused due to the greater free and albumin-bound fractions of circulating T₃ compared to T₄. The patients with Graves' disease showed significantly higher nail T₃ and T₄ concentrations than the euthyroid subjects. The patients with hypothyroidism due to Hashimoto's thyroiditis tended to have lower nail T₃ and T₄ concentrations compared to the euthyroid subjects. Thus, the developed method would be useful for evaluating the medium-to-long term status of thyroid hormone production and has sufficient clinical applicability.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1269 ","pages":"Article 124869"},"PeriodicalIF":2.8,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145621748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Per- and polyfluoroalkyl substances (PFAS) in marine sediments from a Norwegian fjord near an airport: Historical records, distribution, and controlling factors","authors":"Stepan Boitsov,&nbsp;Jon Albretsen,&nbsp;Hilde Elise Heldal,&nbsp;Stig Valdersnes,&nbsp;Bjørn Einar Grøsvik,&nbsp;Bashir Abdulkader,&nbsp;Aasim Ali","doi":"10.1016/j.jchromb.2025.124868","DOIUrl":"10.1016/j.jchromb.2025.124868","url":null,"abstract":"<div><div>Airports have been identified as one of numerous sources of extremely persistent, toxic and bioaccumulative <em>per</em>- and polyfluoroalkyl substances (PFAS), as part of aqueous film-forming foams (AFFF) during fire drills. The historically strongly contaminated terrestrial environment around Flesland airport in Bergen, Norway, raised concern for potential contamination in the surrounding fjords. To investigate this, surface sediments and sediment cores were collected from several locations adjacent to the airport area. One of the cores was dated by ²¹⁰Pb. Two analytical methods, a target method for 24 specific PFAS compounds in marine sediments, and a total oxidizable precursor (TOP) assay method, were established, validated and applied to the samples. The results were combined with previously collected data on surface sediments from fjords and open sea areas in Western Norway. While TOP assay results for sediments and pore water indicated a significant PFAS precursor load at locations close to the airport, and sediment cores showed a continuous increase in PFAS levels (since at least 1980s in the dated core), relatively low levels of PFAS were found in marine sediments off the airport area (1.1 μg/kg dry weight or less for the sum of nine most abundant PFAS in surface sediments). The results agree with earlier data on PFAS background levels in marine sediments from unaffected areas and imply a dispersion of the contamination from the airport elsewhere. Sea current modeling and grain size determination suggest local hydrodynamical and sedimentological conditions as the main factors of influence for the contamination status in the area.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1269 ","pages":"Article 124868"},"PeriodicalIF":2.8,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145663535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation between Geenius™ dual-path migration immunochromatography platform and Western blot HIV-1 for confirmatory assays in the Brazilian population","authors":"Rubia Heloisa Vanderlinde,&nbsp;Graziele Cristina Ferreira,&nbsp;Jussimara Monteiro Nurmberger,&nbsp;Daniela Calciolari Marques,&nbsp;Flavia Maria Correa Cristaldi,&nbsp;Renata Chiavarini,&nbsp;Debora Ribeiro Ramadan,&nbsp;Sergio Tufik,&nbsp;Carolina Xavier Lima Piccinini,&nbsp;Mariana do Nascimento Viana","doi":"10.1016/j.jchromb.2025.124885","DOIUrl":"10.1016/j.jchromb.2025.124885","url":null,"abstract":"<div><div>Human immunodeficiency virus (HIV) diagnosis requires both screening and confirmatory assays to accurately differentiate antigen and antibody reactivity. The Western blot (WB) method has long been considered the gold standard for confirming HIV infection. However, due to its labor-intensive workflow and technical complexity, the WB has become challenging for many clinical laboratories. Therefore, newer diagnostic techniques, such as the Dual-Path Platform (DPP) lateral flow immunochromatography assays, have been developed and approved by regulatory agencies. Automated interpretation of DPP enhances analytical sensitivity by minimizing subjectivity and allowing detection of faint bands, reducing the probability of false-negative results. This study compared the performance of the WB and DPP as confirmatory methods following chemiluminescent immunoassay (CLIA) screening. All samples were previously screened using CLIA and subsequently analyzed by both WB and DPP assays. Samples yielding borderline CLIA results underwent additional testing by RT-qPCR. The DPP platform demonstrated high concordance with the WB assay, achieving an agreement rate of 97.2 % and a kappa index of 93.9 %, indicating excellent predictive performance. Borderline results were confirmed as reactive by both DPP and RT-qPCR but remained indeterminate by WB. Although complete agreement between both confirmatory methods was not achieved, no clinically significant discrepancies were observed, suggesting that such variations did not impact patient outcomes. Despite the WB remaining as a reference confirmatory method for HIV diagnosis, the DPP method offers substantial advantages, including shorter turnaround time, greater operational simplicity, and automated digital documentation, making it a practical alternative for routine use in high-throughput or resource-limited laboratory settings.</div></div>","PeriodicalId":348,"journal":{"name":"Journal of Chromatography B","volume":"1269 ","pages":"Article 124885"},"PeriodicalIF":2.8,"publicationDate":"2026-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145673384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}