Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology最新文献_第7页

Specific patterns of histone marks accompany X chromosome inactivation in a marsupial. 组蛋白标记的特定模式伴随X染色体失活的有袋动物。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2009-01-01 Epub Date: 2009-02-13 DOI: 10.1007/s10577-009-9020-7

Edda Koina, Julie Chaumeil, Ian K Greaves, David J Tremethick, Jennifer A Marshall Graves

{"title":"Specific patterns of histone marks accompany X chromosome inactivation in a marsupial.","authors":"Edda Koina, Julie Chaumeil, Ian K Greaves, David J Tremethick, Jennifer A Marshall Graves","doi":"10.1007/s10577-009-9020-7","DOIUrl":"https://doi.org/10.1007/s10577-009-9020-7","url":null,"abstract":"The inactivation of one of the two X chromosomes in female placental mammals represents a remarkable example of epigenetic silencing. X inactivation occurs also in marsupial mammals, but is phenotypically different, being incomplete, tissue-specific and paternal. Paternal X inactivation occurs also in the extraembryonic cells of rodents, suggesting that imprinted X inactivation represents a simpler ancestral mechanism. This evolved into a complex and random process in placental mammals under the control of the XIST gene, involving notably variant and modified histones. Molecular mechanisms of X inactivation in marsupials are poorly known, but occur in the absence of an XIST homologue. We analysed the specific pattern of histone modifications using immunofluorescence on metaphasic chromosomes of a model kangaroo, the tammar wallaby. We found that all active marks are excluded from the inactive X in marsupials, as in placental mammals, so this represents a common feature of X inactivation throughout mammals. However, we were unable to demonstrate the accumulation of inactive histone marks, suggesting some fundamental differences in the molecular mechanism of X inactivation between marsupial and placental mammals. A better understanding of the epigenetic mechanisms underlying X inactivation in marsupials will provide important insights into the evolution of this complex process.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"115-26"},"PeriodicalIF":2.6,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-009-9020-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27984089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 50

Microdissection and chromosome painting of X and B chromosomes in Locusta migratoria. 迁徙蝗X、B染色体的显微解剖与染色体染色。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2009-01-01 Epub Date: 2008-12-23 DOI: 10.1007/s10577-008-9001-2

María Teruel, Josefa Cabrero, Eugenia E Montiel, Manuel J Acosta, Antonio Sánchez, Juan Pedro M Camacho

{"title":"Microdissection and chromosome painting of X and B chromosomes in Locusta migratoria.","authors":"María Teruel, Josefa Cabrero, Eugenia E Montiel, Manuel J Acosta, Antonio Sánchez, Juan Pedro M Camacho","doi":"10.1007/s10577-008-9001-2","DOIUrl":"https://doi.org/10.1007/s10577-008-9001-2","url":null,"abstract":"Acquisition of knowledge of the nature and DNA content of B chromosomes has been triggered by a collection of molecular techniques, one of which, microdissection, has provided interesting results in a number of B chromosome systems. Here we provide the first data on the molecular composition of B chromosomes in Locusta migratoria, after microdissection of the B and X chromosomes, DNA amplification by one (B) or two (X) different methods, and chromosome painting. The results showed that B chromosomes share at least two types of repetitive DNA sequences with the A chromosomes, suggesting that Bs in this species most likely arose intraspecifically. One of these repetitive DNAs is located on the heterochromatic distal half of the B chromosome and in the pericentromeric regions of about half of the A chromosomes, including the X. The other type of repetitive DNA is located interspersedly over the non-centromeric euchromatic regions of all A chromosomes and in an interstitial part of the proximal euchromatic half of the B chromosome. Chromosome painting, however, did not provide results sufficiently reliable to determine, in this species, which A chromosome gave rise to the B; this might be done by detailed analysis of the microdissected DNA sequences.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"11-8"},"PeriodicalIF":2.6,"publicationDate":"2009-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-008-9001-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"27918518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Avian sex chromosomes: dosage compensation matters. 鸟类性染色体：剂量补偿问题。

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2009-01-01 DOI: 10.1007/s10577-009-9056-8

Heather A McQueen, Michael Clinton

引用次数: 0

miRNA and piRNA localization in the male mammalian meiotic nucleus. 雄性哺乳动物减数分裂核中miRNA和piRNA的定位。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2008-01-01 Epub Date: 2008-01-22 DOI: 10.1007/s10577-007-1190-6

E Marcon, T Babak, G Chua, T Hughes, P B Moens

{"title":"miRNA and piRNA localization in the male mammalian meiotic nucleus.","authors":"E Marcon, T Babak, G Chua, T Hughes, P B Moens","doi":"10.1007/s10577-007-1190-6","DOIUrl":"https://doi.org/10.1007/s10577-007-1190-6","url":null,"abstract":"During mammalian meiosis, transcriptional silencing of the XY bivalent is a necessary event where defects may lead to infertility in males. While not well understood, the mechanism of meiotic gene silencing is believed to be RNA-dependent. In this study, we investigated the types and localization of non-coding RNAs in the meiotic nucleus of the male mouse using a microarray screen with different cell isolates as well as FISH. We report that the dense body, a component of the murine spermatocyte sex body similar to that of a dense body in Chinese hamster spermatocytes, is DNA-negative but rich in proteins and RNA including miRNAs (micro RNAs) and piRNAs (PIWI associated small RNAs), or their precursors. Selective miRNAs and piRNAs localize to chromosome cores, telomeres and the sex body of spermatocytes. These RNAs have not previously been detected in meiotic nuclei. These RNAs appear to associate with the nucleolus of the Sertoli cells as well as with the dense body. While in MIWI-null male mice the nucleolar signal from miRNA and piRNA probes in Sertoli cells is largely diminished, a differential regulation must exist in meiotic nuclei since the localization of these two components appears to be unaffected in the null animal.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"243-60"},"PeriodicalIF":2.6,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-007-1190-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41072414","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 122

In human pachytene spermatocytes, SUMO protein is restricted to the constitutive heterochromatin. 在人粗线精母细胞中，SUMO蛋白仅限于组成异染色质。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2008-01-01 Epub Date: 2008-07-04 DOI: 10.1007/s10577-008-1225-7

Catherine Metzler-Guillemain, Danielle Depetris, Judith J Luciani, Cecile Mignon-Ravix, Michael J Mitchell, Marie-Genevieve Mattei

{"title":"In human pachytene spermatocytes, SUMO protein is restricted to the constitutive heterochromatin.","authors":"Catherine Metzler-Guillemain, Danielle Depetris, Judith J Luciani, Cecile Mignon-Ravix, Michael J Mitchell, Marie-Genevieve Mattei","doi":"10.1007/s10577-008-1225-7","DOIUrl":"https://doi.org/10.1007/s10577-008-1225-7","url":null,"abstract":"SUMO-1, a ubiquitin-like protein, is covalently bound to many proteins, leading to chromatin inactivation and transcriptional repression. The high concentration of SUMO-1 on the XY body in rodents suggests that this protein has an important role in facultative heterochromatin organization. In human, the precise role of SUMO in chromatin/heterochromatin organization remains to be defined. Here we describe the SUMO-1 distribution, during human male meiosis, in relation to the formation of the different types of heterochromatin. We show that, during late pachynema, SUMO-1 appears on the constitutive heterochromatin, but is excluded from the XY body facultative heterochromatin. At the SUMO-1 labelled areas, the presence of HP1alpha protein, as well as of trimethylated H3-K9 and H4-K20 histone modifications, supports a role for SUMO-1 in constitutive heterochromatin organization. We also establish that, on the constitutive heterochromatin, H4-K20me3 staining progressively decreases as SUMO-1 staining increases, suggesting that core histone(s), and histone H4 in particular, are direct targets for sumoylation. Our results also suggest that, in the context of global histone H4 hyperacetylation that precedes the histone-to-protamine transition at postmeiotic stages of spermatogenesis, histone H4 sumoylation may represent an important epigenetic marker replacing methylation on the constitutive heterochromatin.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"761-82"},"PeriodicalIF":2.6,"publicationDate":"2008-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-008-1225-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40524738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Inhibition of transcription at radiation-induced nuclear foci of phosphorylated histone H2AX in mammalian cells. 哺乳动物细胞中磷酸化组蛋白H2AX在辐射诱导的核病灶处的转录抑制。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-08-23 DOI: 10.1007/s10577-007-1162-x

Liudmila V Solovjeva, Maria P Svetlova, Vadim O Chagin, Nikolai V Tomilin

{"title":"Inhibition of transcription at radiation-induced nuclear foci of phosphorylated histone H2AX in mammalian cells.","authors":"Liudmila V Solovjeva, Maria P Svetlova, Vadim O Chagin, Nikolai V Tomilin","doi":"10.1007/s10577-007-1162-x","DOIUrl":"https://doi.org/10.1007/s10577-007-1162-x","url":null,"abstract":"Double-strand DNA breaks (DSBs) induced by ionizing radiation can be visualized in human cells using antibodies against Ser-139 phosphorylated histone H2AX (gamma-H2AX). Large gamma-H2AX foci are seen in the nucleus fixed 1 hour after irradiation and their number corresponds to the number of DSBs, allowing analysis of these genome lesions after low doses. We estimated whether transcription is affected in chromatin domains containing gamma-H2AX by following in vivo incorporation of 5-bromouridine triphosphate (BrUTP) loaded by cell scratching (run-on assay). We found that BrUTP incorporation is strongly suppressed at gamma-H2AX foci, suggesting that H2AX phosphorylation inhibits transcription. This is not caused by preferential association of gamma-H2AX foci with constitutive or facultative heterochromatin, which was visualized in irradiated cells using antibodies against histone H3 trimethylated at lysine-9 (H3-K9m3) or histone H3 trimethylated at lysine-27 (H3-K27m3). Apparently, formation of gamma-H2AX induces changes of chromatin that inhibit assembly of transcription complexes without heterochromatin formation. Inhibition of transcription by phosphorylation of histone H2AX can decrease chromatin movement at DSBs and frequency of misjoining of DNA ends.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"787-97"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-007-1162-x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40977726","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 47

Generation of an improved cytogenetic and comparative map of Bos taurus chromosome BTA27. 牛BTA27染色体改进的细胞遗传学和比较图谱的生成。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-01-09 DOI: 10.1007/s10577-006-1113-y

Tom Goldammer, Ronald M Brunner, Rosemarie Weikard, Christa Kuehn, Klaus Wimmers

{"title":"Generation of an improved cytogenetic and comparative map of Bos taurus chromosome BTA27.","authors":"Tom Goldammer, Ronald M Brunner, Rosemarie Weikard, Christa Kuehn, Klaus Wimmers","doi":"10.1007/s10577-006-1113-y","DOIUrl":"https://doi.org/10.1007/s10577-006-1113-y","url":null,"abstract":"Comparative genome analysis in cattle, human, and mouse identified various evolutionary breakpoints between Bos taurus 27 chromosome (BTA27) and corresponding segments in the Homo sapiens 4 and 8 chromosomes (HSA4, HSA8) and the Mus musculus 8 chromosome (MMU8). The fragmentary cytogenetic location of breaks is based on nine known loci and Zoo-FISH data on BTA27. A comparative mapping approach combining in-silico mapping and physical mapping by fluorescence in-situ hybridization (FISH) revealed an improved cytogenetic map of BTA27 based on 25 new and nine existing assignments of loci. Furthermore, hybrid cell mapping techniques identified and anchored three additional gene loci on BTA27. The BTA27 map was compared with available mapping and annotated sequence data for the chromosome and a generated comparative map displays conserved syntenic chromosome blocks between cattle, human, and mouse. The new anchor loci identify and narrow down evolutionary breakpoints on a cytogenetic level and can help to support the cattle genome assembly and annotation process.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"203-13"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-006-1113-y","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26474192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Phosphorylation of the histone H3.3 variant in mitosis and meiosis of the urochordate Oikopleura dioica. 组蛋白H3.3变异在尾脊索鱼有丝分裂和减数分裂中的磷酸化作用。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-02-15 DOI: 10.1007/s10577-006-1112-z

Alexandra Schulmeister, Martina Schmid, Eric M Thompson

{"title":"Phosphorylation of the histone H3.3 variant in mitosis and meiosis of the urochordate Oikopleura dioica.","authors":"Alexandra Schulmeister, Martina Schmid, Eric M Thompson","doi":"10.1007/s10577-006-1112-z","DOIUrl":"https://doi.org/10.1007/s10577-006-1112-z","url":null,"abstract":"Mammalian histone variant H3.3 differs from replication-dependent histone H3.1 by five amino acids, including replacement of alanine 31 by serine. H3.3 is expressed throughout the cell cycle, primarily deposited at transcriptionally active loci independent of S-phase. Data from mammalian cells suggest that phosphorylation of serine 31 (H3.3S31P) plays a role in mitosis. Here we show that H3.3S31P also occurs during mitosis of the urochordate Oikopleura dioica, suggesting this histone modification and its function in mitosis is already present at the invertebrate-vertebrate transition. The spatial pattern differed from that of H3 phosphorylation at serine 28 (H3S28P). H3S28P was enriched near telomeric regions, but H3.3S31P differed both temporally and spatially from the mammalian pattern, being more widely distributed throughout prophase, prometaphase and metaphase chromosomes. We also identified an important role for H3.3S31P during oogenic meiosis in the semelparous O. dioica. H3.3S31P initiated together with H3S28P in all meiotic nuclei in late diplotene, after H3S10P. However, H3.3S31P was retained only on the subset of meiotic nuclei that seeded maturing oocytes and proceeded through meiosis to arrest in metaphase I. Thus, this epigenetic mark is part of a regulatory circuitry that enables O. dioica to numerically adjust oocyte production over two orders of magnitude.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"189-201"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-006-1112-z","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26581030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

The region homologous to the X-chromosome inactivation centre has been disrupted in marsupial and monotreme mammals. 在有袋类和单孔类哺乳动物中，x染色体失活中心的同源区域已被破坏。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-03-05 DOI: 10.1007/s10577-007-1119-0

Timothy A Hore, Edda Koina, Matthew J Wakefield, Jennifer A Marshall Graves

{"title":"The region homologous to the X-chromosome inactivation centre has been disrupted in marsupial and monotreme mammals.","authors":"Timothy A Hore, Edda Koina, Matthew J Wakefield, Jennifer A Marshall Graves","doi":"10.1007/s10577-007-1119-0","DOIUrl":"https://doi.org/10.1007/s10577-007-1119-0","url":null,"abstract":"Marsupial, as well as eutherian, mammals are subject to X chromosome inactivation in the somatic cells of females, although the phenotype and the molecular mechanism differ in important respects. Monotreme mammals appear to subscribe at least to a form of dosage compensation of X-borne genes. An important question is whether inactivation in these non-eutherian mammals involves co-ordination by a control locus homologous to the XIST gene and neighbouring genes, which play a key regulatory role in human and mouse X inactivation. We mapped BACs containing several orthologues of protein-coding genes that flank human and mouse XIST and genes that lie in the homologous region in chicken and frog. We found that these genes map to two distant locations on the opossum X, and also to different locations on a platypus autosome. We failed to find any trace of an XIST orthologue in any marsupial or monotreme or on any flanking BAC, confirming the conclusion from recent work that non-eutherian mammals lack XIST. We propose the region homologous to the human and mouse X-inactivation centre expanded in early mammals, and this unstable region was disrupted independently in marsupial and monotreme lineages. In the eutherian lineage, inserted and existing sequences provided the starting material for the non-translated RNAs of the X-inactivation centre, including XIST.","PeriodicalId":347802,"journal":{"name":"Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology","volume":" ","pages":"147-61"},"PeriodicalIF":2.6,"publicationDate":"2007-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1007/s10577-007-1119-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"26581032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 98

Systems biology meets chromatin function: a report on the Fourth Elmau Conference on Nuclear Organization. 系统生物学满足染色质功能:第四届埃尔茂核组织会议报告。

IF 2.6

Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology Pub Date : 2007-01-01 Epub Date: 2007-01-26 DOI: 10.1007/s10577-006-1118-6

Christophe Lavelle, Alex Sigal

引用次数: 1