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The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.最新文献

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A micromanipulation platform based on magnetic tweezers and total internal reflection fluorescence microscopy for cell mechanical studies 基于磁镊和全内反射荧光显微镜的细胞力学研究微操作平台
The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004. Pub Date : 2004-12-15 DOI: 10.1109/APBP.2004.1412350
Chaug-Chun Lee, Cheng-hao Chien, Liang-Kung Wen, C. Dong
{"title":"A micromanipulation platform based on magnetic tweezers and total internal reflection fluorescence microscopy for cell mechanical studies","authors":"Chaug-Chun Lee, Cheng-hao Chien, Liang-Kung Wen, C. Dong","doi":"10.1109/APBP.2004.1412350","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412350","url":null,"abstract":"We developed a manipulation platform, which combines magnetic tweezers and the incubating system, with total internal reflection fluorescence microscopy (TIRFM). Our system can generate forces up to the nN range and such forces can be used to observe and characterize cell mechanical responses to external forces. Combined with TIRFM, cell surface stress-strain phenomena can also be investigated.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"122 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123477125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Merocyanine 540-mediated photodynamic therapy against Staphylococcus aureus planktonic and biofilm cells Merocyanine 540介导的对金黄色葡萄球菌浮游和生物膜细胞的光动力疗法
The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004. Pub Date : 2004-12-15 DOI: 10.1109/APBP.2004.1412290
Hsiao-Yin Lin, Chin-Tin Chen, Ching-Tsan Huang
{"title":"Merocyanine 540-mediated photodynamic therapy against Staphylococcus aureus planktonic and biofilm cells","authors":"Hsiao-Yin Lin, Chin-Tin Chen, Ching-Tsan Huang","doi":"10.1109/APBP.2004.1412290","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412290","url":null,"abstract":"Photodynamic inactivation of Staphylococcus aureus planktonic and biofilm cells by a photosensitizer, merocyanine 540 (MC 540) was investigated. For the planktonic experiments, the antimicrobial activity was enhanced with the increasing light dose, but an increase in light dose could not further improve the antimicrobial activity if the maximum excitation level attainable lay below the necessary minimum threshold level. A complete inactivation was achieved when the excitation level of MC 540 lay somewhere above the threshold level. The relationship between antimicrobial activity and the excitation level of MC 540 revealed that the more MC 540 was excited, the more S. aureus cells were killed. For the biofilm experiments, the antimicrobial activity was enhanced with the increasing light dose. No viable cells were detected when exposed to 15 /spl mu/g/ml MC 540 and a light dose of 600 J/cm/sup 2/ or 20 /spl mu/g/ml MC 540 and a light dose of 450 J/cm/sup 2/. The results from both planktonic and biofilm experiments suggest that the antimicrobial activity of photodynamic inactivation against S. aureus is closely related to the excitation level of MC 540.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126227666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Full-field optical coherence tomography with achromatic phase shifter 带消色差移相器的全场光学相干层析成像
The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004. Pub Date : 2004-12-15 DOI: 10.1109/APBP.2004.1412315
Y. Watanabe, Y. Hayasaka, M. Sato
{"title":"Full-field optical coherence tomography with achromatic phase shifter","authors":"Y. Watanabe, Y. Hayasaka, M. Sato","doi":"10.1109/APBP.2004.1412315","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412315","url":null,"abstract":"We demonstrated full-field optical coherence tomography (OCT) using achromatic phase shifting with a rotating polarizer. Using this phase shifter and conventional phase shift method, we obtained en-face OCT images of an onion at different depths.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131079383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Spatiotemporal diffuse reflectometry 时空漫反射计
The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004. Pub Date : 2004-12-15 DOI: 10.1109/APBP.2004.1412309
T. Iwai
{"title":"Spatiotemporal diffuse reflectometry","authors":"T. Iwai","doi":"10.1109/APBP.2004.1412309","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412309","url":null,"abstract":"We have so far concerned with developing diffuse reflectometry for static and dynamic light back-scattered multiply from extremely dense media to characterizing scatters in size, shape, the state of aggregation, and the absorption. In this report, we will introduce our researches on applications of multiple backscattering phenomena to new diffuse reflectometry.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"22 1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123102635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dorsal skinfold titanium chamber for noninvasive imaging in nude mice using multiphoton and harmonic generation microscopy 用多光子和谐波产生显微镜对裸鼠背部皮肤褶钛腔进行无创成像
The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004. Pub Date : 2004-12-15 DOI: 10.1109/APBP.2004.1412343
Feng-Chieh Li, Chun-Chin Wang, Sung-Jan Lin, S. Jee, C. Dong
{"title":"Dorsal skinfold titanium chamber for noninvasive imaging in nude mice using multiphoton and harmonic generation microscopy","authors":"Feng-Chieh Li, Chun-Chin Wang, Sung-Jan Lin, S. Jee, C. Dong","doi":"10.1109/APBP.2004.1412343","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412343","url":null,"abstract":"For many years, the dorsal skinfold chamber has been used for intravital observation in animal models. We have developed a chamber built using medical-grade titanium. Our design allows long-term, high resolution, and large-area observation in nude mice in vivo. Combined with two-photon fluorescence and second-harmonic generation microscopy, our chamber can be used to obtain qualitative and quantitative information for in vivo analysis of diseases such as tumors.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127717486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Multiphoton imaging of porcine eye: cornea, limbus, conjunctiva, and sclera 猪眼的多光子成像:角膜、角膜缘、结膜和巩膜
The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004. Pub Date : 2004-12-15 DOI: 10.1109/APBP.2004.1412342
S. Teng, Ju-Li Peng, H. Lin, Hai-Yin Wu, Wen Lo, Yen Sun, Wei-Chou Lin, Sung-Jan Lin, S. Jee, H. Tan, C. Dong
{"title":"Multiphoton imaging of porcine eye: cornea, limbus, conjunctiva, and sclera","authors":"S. Teng, Ju-Li Peng, H. Lin, Hai-Yin Wu, Wen Lo, Yen Sun, Wei-Chou Lin, Sung-Jan Lin, S. Jee, H. Tan, C. Dong","doi":"10.1109/APBP.2004.1412342","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412342","url":null,"abstract":"This purpose of this study is to demonstrate the feasibility of using multiphoton microscopy in imaging eye surface. Specifically, the cornea, limbus, conjunctiva, and sclera were imaged using multiphoton induced fluorescence and second-harmonic generation (SHG) imaging.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133725488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Experimental study of pulsed ultrasono-optical tomography 脉冲超声光学断层扫描的实验研究
The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004. Pub Date : 2004-12-15 DOI: 10.1109/APBP.2004.1412375
Y. Kunihiro, K. Kagei, M. Fujii, K. Nakayama
{"title":"Experimental study of pulsed ultrasono-optical tomography","authors":"Y. Kunihiro, K. Kagei, M. Fujii, K. Nakayama","doi":"10.1109/APBP.2004.1412375","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412375","url":null,"abstract":"We present a phantom study of NIR tomographic imaging based on the pulsed-ultrasound modulation of diffuse light in turbid media. The bottleneck of the method is the very low S/N which cannot be overcome by the conventional signal-averaging because of the slowly-varying light phase on the detector. We proposed a simple model for the detected signal and validated it experimentally. Furthermore, we proposed the frequency-doubling-and-averaging method to recover the signal, and demonstrated its feasibility through the phantom experiment.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114698079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Surface enhanced Raman spectra in biochemical molecules and cell detection 表面增强拉曼光谱在生化分子和细胞检测中的应用
The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004. Pub Date : 2004-12-15 DOI: 10.1109/APBP.2004.1412276
Tsuei-Lian Wang, Fang-Ying Peng, Hui-Hisn Lu, Yung-Ta Hung, Tung-Jing Fang, H. Chiang
{"title":"Surface enhanced Raman spectra in biochemical molecules and cell detection","authors":"Tsuei-Lian Wang, Fang-Ying Peng, Hui-Hisn Lu, Yung-Ta Hung, Tung-Jing Fang, H. Chiang","doi":"10.1109/APBP.2004.1412276","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412276","url":null,"abstract":"This work presents the quantitative detection of creatinine molecules and SERS signal from a single cell. Through the utilization of gold nanoparticles, we can measure the lower concentration of specimens. In biochemical molecules analysis, quantification of creatinine in human urine sample is demonstrated. The correlation coefficient can be reached as high as 0.99 for mixture sample, and 0.93 for human subject analysis.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114345262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Balanced detection optical heterodyne polarimeter for optical activity measurement of a quartz crystal 用于石英晶体光学活度测量的平衡检测光学外差偏振计
The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004. Pub Date : 2004-12-15 DOI: 10.1109/APBP.2004.1412352
C. Chou, H. Tsai, Kuan-Yung Liao, Li-dek Chou
{"title":"Balanced detection optical heterodyne polarimeter for optical activity measurement of a quartz crystal","authors":"C. Chou, H. Tsai, Kuan-Yung Liao, Li-dek Chou","doi":"10.1109/APBP.2004.1412352","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412352","url":null,"abstract":"A novel amplitude sensitive optical heterodyne polarimeter in which a Zeeman laser associates with a balanced detector scheme to measure the optical activity |n/sub r/-n/sub l/ of a quartz crystal is proposed and experimentally demonstrated. The sensitivity of the measurement to 8.5/spl times/10/sup -10/ is analyzed.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"94 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124699783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Fiber optic evanescent wave biosensor for biomolecular detection 用于生物分子检测的光纤倏逝波生物传感器
The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004. Pub Date : 2004-12-15 DOI: 10.1109/APBP.2004.1412333
Sheng-Hong Yu, Cheng-Hau Chen, H. Chiang
{"title":"Fiber optic evanescent wave biosensor for biomolecular detection","authors":"Sheng-Hong Yu, Cheng-Hau Chen, H. Chiang","doi":"10.1109/APBP.2004.1412333","DOIUrl":"https://doi.org/10.1109/APBP.2004.1412333","url":null,"abstract":"An evanescent wave fluorescence-based fiber-optic biosensor is developed for high sensitivity, low cost, compact design and real-time in-situ monitoring. In the research, we accomplished the fiber-optical biosensor system to monitor the reaction of IgG-Anti rabbit IgG and Biotin-Streptavidin, and the kinetic association and dissociation constant of target analytes were calculated.","PeriodicalId":346624,"journal":{"name":"The Second Asian and Pacific Rim Symposium on Biophotonics, 2004. APBP 2004.","volume":"1996 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2004-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123785120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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