{"title":"Etnobotani Kopi di Desa Bambang Kecamatan Wajak Kabupaten Malang","authors":"Nasrul Jauhari, L. Hakim, Hasan Zayadi","doi":"10.33474/j.sa.v4i2.12386","DOIUrl":"https://doi.org/10.33474/j.sa.v4i2.12386","url":null,"abstract":"This study aims to determine the types and characterization of coffee in Bambang Village and the pattern of cultivation and utilization of coffee in Bambang Village, Malang Regency. The method used in this study is observation and semi-structured interviews. Qualitative data analysis is presented in descriptive form. There are three types of coffee that are cultivated in Bambang Village, namely Coffea canephora, Coffea liberica and Coffea arabica. There are three constituent layers in coffee agroforestry, the top layer functions as a canopy consisting of Kaliandra, Jackfruit, Waru, Avocado, Lamtoro, Petai, Coconut. The middle layer consists of coffee, papaya and banana and the lower layer consists of taro, ginger and tubers. The complexity of making up coffee agroforestry is used as animal feed, firewood reserves, food sources, nutritional sources and building wood materials and the form of coffee utilization as a drink is often used as a daily dish, guest treats, arts, celebrations and traditional rituals.Keywords: Ethnobotany, Types of Coffee, Coffee AgroforestryABSTRAKPenelitian ini bertujuan untuk mengetahui jenis karakteristik kopi lokal dan pola budidaya serta pemanfaatan kopi yang ada di Desa Bambang Kabupaten Malang. Metode yang digunakan dalam penelitian ini adalah observasi dan wawancara semi terstruktur. Analisis data kualitatif disajikan dalam bentuk deskriptif. Terdapat tiga jenis kopi yang dibudidayakan di Desa Bambang yaitu jenis Coffea canephora, Coffea liberica dan Coffea arabica. Ada tiga lapisan penyusun di dalam agroforestri kopi Desa Bambang, lapisan atas berfungsi sebagai kanopi terdiri dari Kaliandra, Nangka, Waru, Alpukat, Lamtoro, Petai, Kelapa. Lapisan tengah terdiri dari Kopi, Pepaya dan Pisang dan lapisan bawah terdiri dari Talas, Jahe dan Umbi-umbian. Kompleksitas penyusun agroforestri kopi dimanfaatkan sebagai pakan ternak, cadangan kayu bakar, sumber makanan, sumber nutrisi dan bahan kayu bangunan dan bentuk pemanfaatan kopi sebagai minuman sering digunakan sebagai sajian harian, suguhan tamu, kesenian, selamatan dan ritual adat.Kata kunci : Etnobotani, Jenis-Jenis Kopi, Agroforestri Kopi","PeriodicalId":346598,"journal":{"name":"Jurnal SAINS ALAMI (Known Nature)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134574735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Kadar Albumin Ikan Glodok Segar Boleophthalmus boddarti dengan Metode Biuret","authors":"Farah Nabila Belqis, Hari Santosa, Ahmad Syauqi","doi":"10.33474/j.sa.v4i2.10373","DOIUrl":"https://doi.org/10.33474/j.sa.v4i2.10373","url":null,"abstract":"one of which is mudskipper. The species Boleophthalmus boddarti is able to survive outside the water for long periods, spending time outside the surface of the water walking, wallowing in mud, and entering burrows it creates. The purpose of this study was to analyze the albumin levels in fresh mudskipper meat of Boleophthalmus boddarti species using the biuret method. The research method is descriptive and is repeated three times. Using the biuret method with the spectrophotometer technique the standard curve equation y = 0.0482x + 0.0036. The highest albumin yield was found in the head meat, weighing 0.085 g with the absorbance in the spectrophotometer was 92.80 nm. Compared with the tail meat, the weight of albumin is 0.080 g and the absorbance is 70.08 nm. The greater the absorbance effect on albumin levels, the average measurement of albumin content of mudskipper species Boleophthalmus boddarti, the highest was found in the head meat, namely 93.667% and the albumin content in the meat of the tail was lower, namely 65.97%.Keywords: Albumin, Protein, Boleophthalmus boddarti ABSTRAK Albumin merupakan komponen protein dalam plasma yang dapat larut dalam air. Albumin dapat di jumpai dalam sebagian besar protein dalam ikan, salah satunya yaitu ikan glodok. Spesies Boleophthalmus boddarti mampu bertahan hidup di luar perairan dalam waktu yang lama, menghabiskan waktunya di luar permukaan air dengan berjalan, berkubang di lumpur, dan memasuki liang yang dibuatnya. Tujuan dari penelitian ini adalah untuk menganalisa kadar albumin pada daging ikan glodok segar spesies Boleophthalmus boddarti dengan metode biuret. Metode penelitian adalah deskriptif dan pengulangan tiga kali. Menggunakan metode biuret dengan teknik spektrofotometer persamaan kurva standart y = 0,0482x + 0,0036. Hasil albumin tertinggi di dapatkan pada bagian daging kepala yaitu seberat 0,085 gr dengan absorbansi dalam spektofotometer adalah 92,80 nm. Dibandingkan dengan bagian daging ekornya berat albumin yang di dapatkan seberat 0,080 gr dan absorbansi 70,08 nm. Semakin besar absorbansi berpengaruh terhadap kadar albumin, rata – rata pengukuran kadar albumin ikan glodok spesies Boleophthalmus boddarti paling tinggi terdapat pada daging bagian kepala yaitu 93,667 % dan kadar albumin pada daging bagian ekornya lebih rendah yaitu 65,97 %. Kata kunci : Albumin, Protein, Boleophthalmus boddarti","PeriodicalId":346598,"journal":{"name":"Jurnal SAINS ALAMI (Known Nature)","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132251042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pengaruh Pemberian Jamu Herbal Terhadap Pembentukan Otot Tendon Sapi Kerapan Madura (Bos primiginius)","authors":"Achmad Jailani, Hari Santoso, Hasan Zayadi","doi":"10.33474/j.sa.v4i2.11447","DOIUrl":"https://doi.org/10.33474/j.sa.v4i2.11447","url":null,"abstract":"This study aims to determine the effect of giving herbal ingredients on the muscle formation of the tendons of the Madura bullock cattle (Bos primiginius). The method used in this study was carried out by surveys and measurements in the field. Sampling of locations and cattle using purposive sampling, namely bulls are taken at locations that have potential for bullocks. The vital statistics were calculated using the incidental sampling method. The results showed that the provision of herbal concoctions of temu lawak, temu ireng, turmeric, black coffee ginger, galangal, and ginseng can increase stamina so as to speed up running cows, then herbal medicine is significant in the formation of tendon muscles. It is proven that the formation of muscle tendons is correlated with increasing running speed as evidenced by the results of increasing running speed of 17.15/second within a distance of 221 meters so that it can be concluded that herbal medicine is significant for the formation bovine tendon muscles.Keywords: Jamu Herbal, Madura Kerapan Cow, Tendon MusclesABSTRAKPenelitian ini bertujuan untuk mengetahui pengaruh pemberian ramuan herbal terhadap pembentukan otot tendon sapi kerapan Madura (Bos primiginius). Metode yang digunakan dalam penelitian ini dilakukan dengan survey dan pengukuran di lapang. Sampling lokasi dan ternak sapi menggunakan purposive sampling yaitu sapi kerapan diambil pada lokasi yang potensial sapi kerapan. Data dihitung statistik vital menggunakan metode insidental sampling. Hasil didapatkan menunjukkan bahwa Pemberian ramuan herbal temu lawak,temu ireng, kunyit, jahe kopi hitam, lengkuas,dan gingseng dapat menambah stamina sehingga mempercepat lari sapi, maka jamu herbal signifikan dalam pembentukan otot tendon hal ini terbukti bahwa pembentukan otot tendon berkolerasi dengan penambahan kecepatan lari dibuktikan dengan hasil bertambahnya kecepatan lari 17.15/Detik dalam jarak 221 Meter sehingga dapat disimpulkan bahwa jamu herbal signifikan terhadap pembentukan otot tendon sapi kerapan.Kata kunci : Jamu Herbal, Sapi Kerapan Madura, Otot Tendon","PeriodicalId":346598,"journal":{"name":"Jurnal SAINS ALAMI (Known Nature)","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125484624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Uji Efektivitas Daun Maja (Aegle marmelos) Sebagai Bioinsektisida Hama Plutella xylostella pada Tanaman Brokoli (Brassica oleracea var. italica)","authors":"Silvia Fitrotul Azizah, Saimul Laili, Ratna Djuniwati Lisminingsih","doi":"10.33474/j.sa.v4i1.9965","DOIUrl":"https://doi.org/10.33474/j.sa.v4i1.9965","url":null,"abstract":"Indonesia has many insecticide producing plants that can be used as a control of plant pests.. Fermentation of squeeze maja leaves is suspected to be used as an herbal insecticide. Broccoli as a vegetable plant brassicacea tribe, one of the pests destroying broccoli plants is the caterpillar Plutella xylostella. The purpose ofthis research is to find out the effectiveness of fermentation of maja leaves as a pest of Plutella xylostella. Experimental research method is done using randomized design group (RDG) with 5 treatment concentrations sprayed fermentation maja leaves that is P0 = control (0%), P1 (25%), P2 (50%), P3 (75%), P4 (100%) and 4 repeats. Data from the analysis using Anova. The results showed Fhit > F(0.05) which is 11.12 > 3.26 there is a significant influence. But for the treatment of group Fhit < F(0.05) which is 1.15 < 3.49. The temporary conclusion of fermentation spraying of maja leaves has an effect on the mortality of the pest Plutella xylostella, but has no significant effect on the treatment of the group.Keywords: Maja Leaf Fermentation Solution, Herbal Insecticide, Plutella xylostella Pest ABSTRAKIndonesia mempunyai banyak tumbuhan penghasil insektisida yang dapat dimanfaatkan sebagai mengendalikan hama tanaman. Fermentasi perasan daun maja diduga dapat digunakan sebagai bahan insektisida herbal. Brokoli sebagai tanaman sayuran suku brassicacea, salah satu hama perusak tanaman brokoli adalah ulat Plutella xylostella. Tujuan penelitian ini untuk mengetahui efektivitas fermentasi perasan daun maja sebagai mengendali hama Plutella xylostella.Metode penelitian dilakakuan secara eksperimental menggunakan Rancangan Acak Kelompok (RAK) dengan 5 perlakuan konsentrasi yang disemprot fermentasi perasan daun maja yaitu P0 = kontrol (0%), P1 (25%), P2 (50%), P3 (75%), P4 (100%) dan 4 kali ulangan. Data hasil penelitian dianalisis menggunakan Anova. Hasil penelitian menunjukkan Fhit > F(0,05) yaitu 11.12 > 3.26 terdapat pengaruh yang signifikan. Tetapi untuk perlakuan kelompok Fhit < F(0.05) yaitu 1.15 < 3,49. Kesimpulan sementara penyemprotan fermentasi perasan daun maja berpengaruh terhadap mortalitas hama Plutella xylostella, tetapi tidak berpengaruh secara signifikan terhadap perlakuan kelompok.Kata kunci : Larutan Fermentasi Daun Maja, Insektisida Herbal, Hama Plutella xylostella","PeriodicalId":346598,"journal":{"name":"Jurnal SAINS ALAMI (Known Nature)","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133332779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Uji Efektivitas Residu Tembakau Sebagai Bioinsektisida Hama Plutella Xylostella pada Tanaman Sawi (Brassica juncea L.)","authors":"Rina Alfi Hafiana, Saimul Laili, Ratna Djuniwati Lisminingsih","doi":"10.33474/j.sa.v4i1.8895","DOIUrl":"https://doi.org/10.33474/j.sa.v4i1.8895","url":null,"abstract":"The mustard plant (Brassica juncea L) has been widely cultivated by local communities. The problem that occurs in mustard cultivation farmers is the attack of plant pests which can reduce the quality and quantity of mustard plants. The abundant amount of cigarette butts waste has the potential to be used as a source of insecticides. The nicotine in cigarette butts is acknowledge to be a potential nerve poison and is used as a raw material for various types of insecticides and the essential oil content in clove also contains eugenol which is a component that can be used as an inhibitor of bacterial and fungal proliferation and plays an effective role in controlling pests, can cause skin irritation, and other problems that will become contact poison for insect pests. One of the leaf destroying leaf plants is Plutella xylostella. This study aims to distinguish the effect of kretek cigarette butts and determine the effective concentration on pest mortality. This research was carried out at Biology Laboratory, Faculty of Mathematics and Natural Sciences, Islamic University of Malang. The research method used an Randomized Block Design (RBD) experimental with 5 treatments and 4 replications. ANOVA test results show there are significant differences and the pest mortality tend to increase after being treated. The most effective results are 25% solution concentrations with 6.75% mortality. Keywords: Bioinsecticide, Cretek Cigarette Waste, Plutella xylostella Pest ABSTRAKTanaman sawi (Brassica juncea L) telah banyak dibudidayakan oleh masyarakat sekitar. Permasalahan yang terjadi pada petani budidaya sawi adalah serangan hama tanaman yang dapat menurunkan kualitas dan kuantitas tanaman sawi. Limbah puntung rokok jumlahnya yang sangat melimpah berpotensi untuk dimanfatkan sebagai sumber insektisida. Nikotin yang ada di puntung rokok diyakini dapat menjadi racun syaraf yang potensial dan digunakan sebagai bahan baku berbagai jenis insektisida serta kandungan minyak atsiri pada cengkeh juga mengandung eugenol yang merupakan komponen yang dapat digunakan sebagai penghambat perkembangbiakan bakteri dan jamur serta berperan efektif dalam mengendalikan hama, dapat menimbulkan iritasi kulit dan masalah masalah lain yang akan menjadi racun kontak untuk serangga hama. Salah satu hama tanaman sawi perusak daun yaitu Plutella xylostella. Penelitian ini bertujuan untuk membedakan pengaruh limbah puntung rokok kretek dan mengetahui konsentrasi efektif terhadap mortalitas. Penelitian ini dilakukan dilaboratorium Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Islam Malang. Metode penelitian menggunakan eksperimental Rancangan Acak Kelompok (RAK) dengan 5 perlakuan dan 4 ulangan. Hasil uji statistik ANOVA menunjukkan terdapat perbedaan yang signifikan serta cenderung mengalami peningkatan mortalitas hama setelah diberi perlakuan. Hasil yang paling efektif yaitu dengan konsentrasi larutan 25% dengan mortalitas 6,75%. Kata kunci : Bioinsektisida, Li","PeriodicalId":346598,"journal":{"name":"Jurnal SAINS ALAMI (Known Nature)","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124567148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Pengaruh Pemberian Ampas Hasil Fermentasi Buah Maja (Aegle marmelos) Terhadap Pertumbuhan Tanaman Bayam (Amaranthus sp.)","authors":"Diyah Ayu Trisna Mulyani, Saimul Laili, Ratna Djuniwati Lisminingsih","doi":"10.33474/j.sa.v4i1.9987","DOIUrl":"https://doi.org/10.33474/j.sa.v4i1.9987","url":null,"abstract":"Maja is a plant belonging to the Rutaceae family which originates from the tropics. Green spinach (Amaranthus sp.) belongs to the Amaranthaceae family. Spinach is one of the vegetables consumed by people. The purpose of this study was to determine the effect of giving dreg from fermented maja fruit (Aegle marmelos) on the growth of spinach (Amaranthus sp. L) and the dose of dreg from fermented maja fruit (Aegle marmelos) which has the most influence on the growth of pulled spinach (Amaranthus sp. L). By using this research methodology using experimental Completely Randomized Design (CRD). There were 5 treatment combinations with 4 replications so that there were 20 experimental samples, with a treatment combination of 15gr, 30gr, 45gr and 60g. With research procedures the process of sampling maja fruit, seeding, planting, harvesting. Data analysis with analysis of variance (ANOVA), there is a real difference followed by LSD test 5%. For plant height, the average yield was 38.59 cm, the number of leaves was 14.5, root length 33.5 cm, wet weight 19.5 grams, and dry weight 9.35 grams. Provision of fermented dreg has a significant effect on the growth of spinach at a dose of 60 grams / plant. Keywords: Spinach, Maja Fruit Fermentation, Spinach Plant Growth ABSTRAKMaja adalah tanaman yang tergolong dalam family Rutaceae yang berasal dari daerah tropis. Tanaman bayam hijau (Amaranthus sp.) termasuk family Amaranthaceae. Bayam adalah salah satu sayuran yang dikonsumsi masyarakatTujuan penelitian ini adalah untuk mengetahui pengaruh pemberian ampas hasil fermentasi buah maja (Aegle marmelos) terhadap pertumbuhan tanaman bayam cabut (Amaranthus sp. L) dan dosis ampas hasil fermentasi buah maja (Aegle marmelos) yang paling berpengaruh terhadap pertumbuhan tanaman bayam cabut (Amaranthus sp. L). Dengan menggunakan metode penelitian ini menggunakan eksperimental Rancangan Acak Lengkap (RAL). Terdapat 5 kombinasi perlakuan 4 ulangan sehingga terdapat 20 sampel percobaan, dengan kombinasi perlakuan 15gr, 30gr, 45gr dan 60 gr. Dengan prosedur penelitian proses pengambilan sampel buah maja, penyemaian benih, penanaman, panen. Analisis data dengan Analisis of Varian (ANOVA), jika ada perbedaan nyata dilanjut dengan uji BNT 5%. Untuk tinggi tanaman mendapatkan hasil rata-rata 38,59 cm, jumlah daun 14,5 helai, panjang akar 33,5 cm, berat basah 19,5 gram, dan berat kering 9,35gram. Pemberian ampas hasil fermetasi memberikan pengaruh yang nyata terhadap pertumbuhan tanaman bayam dengan dosis 60 gram/ tanaman. Kata kunci : Bayam, Fermentasi Buah Maja, Pertumbuhan Tanaman Bayam","PeriodicalId":346598,"journal":{"name":"Jurnal SAINS ALAMI (Known Nature)","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114356594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Safitri, Saimul Laili, Ratna Djuniwati Lisminingsih
{"title":"Uji Limbah Hasil Fermentasi Buah Maja (Aegle marmelos) Terhadap Pertumbuhan Tanaman Sawi Pakcoy (Brassica rapa l.)","authors":"S. Safitri, Saimul Laili, Ratna Djuniwati Lisminingsih","doi":"10.33474/j.sa.v4i1.8853","DOIUrl":"https://doi.org/10.33474/j.sa.v4i1.8853","url":null,"abstract":"The Maja plant is a plant of the Rutaceae family that from tropical and subtropical regions of America. The benefits of Maja fruit fermentation waste can be used as organic liquid fertilizer. Pakcoy mustard is a vegetable that is needed by humans to meet their daily needs. The need for the vegetable market, especially Pakcoy mustard, has increased from year to year. Fertilizing plants is redundant to replace nutrients transported by plants, especially if the soil used for plant cultivation has a low fertility level, one of which is by utilizing the fermented Maja fruit waste can be used as liquid organic fertilizer containing elements macro and micronutrients needed by plants. This study aims to determine the effect of giving Maja fruit fermentation waste to the growth of mustard Pakcoy and to determine the best concentration for the growth of mustard Pakcoy. The method used in this study was an experimental method using a one-factor completely randomized design study (CRD) with concentrations of 0%, 10%, 20%, 30%, and 40% respectively 4 times with 5 treatments. The parameters observed in this study included plant height, number of leaves, root length, wet weight, dry weight, and abiotic factors. The research data were analyzed using analysis of variance (ANOVA), then if there was a significant difference, the LSD test was continued at 5%. The results showed that giving Maja fruit fermentation waste with a concentration of 40% affected increasing the wet weight of Pakcoy mustard with an average value of 82.50 grams.Keywords: Pakcoy Mustard, Maja Fruit Fermented Waste, Organic FertilizerABSTRAKTanaman maja adalah tumbuhan dari famili Rutaceae berasal dari daerah tropis dan subtropis di Amerika. Manfaat limbah fermentasi buah maja dapat digunakan sebagai pupuk cair organik. Sawi Pakcoy adalah sayuran yang sering diolah oleh manusia untuk memenuhi kebutuhan pangan. Terjadi peningkatan kebutuhan pakcoy setiap periode tahun. Pemberian pupuk terhadap tanaman mutlak diperlukan untuk menggantikan unsur hara. Media tanam budidaya sawi pakcoy pada tanah dengan tingkat kesuburan rendah perlu pemupukan, salah satu alternatif dengan pupuk cair organik limbah fermentasi buah maja yang mudah diperoleh. Limbah tersebut terbukti mengandung unsur hara makro dan mikro yang dibutuhkan tanaman. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian limbah hasil fermentasi buah maja terhadap pertumbuhan tanaman sawi pakcoy pada konsentrasi yang paling baik bagi pertumbuhan. Metode penelitian dilakukan secara eksperimen menggunakan desain rancangan acak lengkap (RAL).Perlakuan konsentrasi dimulai dari 0%; 10%; 20%; 30%; 40% dengan 4 kali ulangan. Pengamatan yang dilakukan pada penelitian ini diantaranya tinggi tanaman, jumlah daun, panjang akar, bobot basah, bobot kering, dan faktor abiotik. Analisis anova digunakan dalam penelitian ini untuk mengetahui adanya perbedaan yang nyata pada setiap perlakuan kemudian dilanjut uji BNT 5%. Penelitian ini memberikan hasil","PeriodicalId":346598,"journal":{"name":"Jurnal SAINS ALAMI (Known Nature)","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123904650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Durrotul Choiroh Choi, Ahmad Syauqi, Ratna Djuniwati Lisminingsih
{"title":"Penambahan Kultur Mikroba Jamur Mikroskopis pada Produk Delignifikasi Material Organik","authors":"Durrotul Choiroh Choi, Ahmad Syauqi, Ratna Djuniwati Lisminingsih","doi":"10.33474/j.sa.v4i1.9912","DOIUrl":"https://doi.org/10.33474/j.sa.v4i1.9912","url":null,"abstract":"Agricultural waste is also known as a berlignocellulosic material composed of three polymers. These polymers can produce valuable products such as fermented sugar. This study aims to study the changes that occur in delignified organic material products added with microscopic fungal cultures and different concentrations. This study used an experimental method with a mean comparison of two populations. Consisting of 2 treatments, the first A: 2.5% (11 replications) and the second B: 7.5% (11 replications), so that the total number of repetitions was 22 times. Using a consortium of Trichoderma viride, Aspergillus niger, Hansenula sp, and Candida sp. The media used in the study were Potato Dextrose Borth (PDB) media, Wang media, and papaya fruit extract. Papaya fruit extract also functions as a nutrient source for mushrooms. The pH used is pH 5. The fermentation process of the media added by delignification products of organic material with consortium fungi for 3 days (72 hours). Determination of glucose levels using the sulfuric-phenolic acid method with an ultra violet (UV) and visible light spectrophotometer. The data analysis used was the T-test. Changes that occur in delignification products of organic material when added with microscopic fungal cultures are the addition of glucose and there is a comparison of glucose levels at different concentrations. In treatment A the average glucose level obtained was 15.72375 mg / ml, while in treatment B the average glucose level obtained was 42.475 mg / ml. This proves that the two treatments were significantly different aimed at P>0.05.Keywords: Delignification, Organic Materia, Microbial Fungi (Trichoderma viride, Aspergillus niger, Hansenula sp., and Candida sp.), GlucoseABSTRAKLimbah pertanian disebut juga sebagai bahan berlignoselulosa tersusun oleh tiga polimer. Polimer tersebut dapat menghasilkan produk yang bernilai seperti gula hasil fermentasi. Penelitian ini memiliki tujuan yaitu untuk mempelajari perubahan yang terjadi pada produk delignifikasi material organik yang ditambahkan kultur jamur mikroskopis dan konsentrasi yang berbeda. Penelitian ini memakai metode eksperimental dengan desain perbandingan rerata dua populasi. Terdiri dari 2 perlakuan, yang pertama A: 2,5% (11 kali ulangan) dan yang kedua B: 7,5% (11 kali ulangan), sehingga jumlah total ulangan ada 22 kali ulangan. Memakai konsorsium jamur Trichoderma viride, Aspergillus niger, Hansenula sp, dan Candida sp. Media yang digunakan pada penelitian yaitu media Potato Dextrose Borth (PDB), media Wang, dan extrak buah pepaya. Ekstrak buah pepaya juga berfungsi sebagai sumber nutrien untuk jamur. pH yang digunakan yaitu pH 5. Proses fermentasi media yang ditambahkan produk delignifikasi material organik dengan jamur konsorsium selama 3 hari (72 jam). Penentuan kadar glukosa memakai metode asam sulfat-fenol dengan alat spektrofotometer sinar ultra violet (UV) dan tampak. Analisis data yang digunakan yaitu Uji-T. Perubahan yang terjadi pada","PeriodicalId":346598,"journal":{"name":"Jurnal SAINS ALAMI (Known Nature)","volume":"86 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115553329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sofiyatul Umaroh, Ahmad Syauqi, Ratna Djuniwati Lisminingsih
{"title":"Uji Antibakteri Ekstrak Eucheuma cottonii Terhadap Bakteri Escherichia coli","authors":"Sofiyatul Umaroh, Ahmad Syauqi, Ratna Djuniwati Lisminingsih","doi":"10.33474/j.sa.v4i1.9909","DOIUrl":"https://doi.org/10.33474/j.sa.v4i1.9909","url":null,"abstract":"Seaweed is a plant as a source of bioactive compounds which produces various kinds of secondary metabolites characterized by a wide spectrum of biological activity. Various benefits of seaweed, namely as a food ingredient, as an ingredient in the pharmaceutical industry and food industry. The content of bioactive compounds in seaweed as secondary metabolites is antibacteria, antioxide, and anticoagulant. Quite a few people know about Escherichia coli, which causes gastrointestinal infections, one of Eucheuma cottonii extract concentration in ethanol as an antibacterial against the growth of Escherichia coli bacteria and to study the maximum inhibition area of Eucheuma cottonii extract against Escherichia coli bacteria. In this study, an experimntal method was used completely randomized design (CRD). It used 4 treatments and 6 replications. By using EMB and BGLB media for antibacterial test. ANOVA test results showed that there was a significant of o%, 19,2%, 35,7%, and 37,5% the results with an average of 3 mm, at a concentration of 37,5% as highest concentration but still in a weak category.Keywords: Eucheuma cottonii, Antibacteria, Escherichia coli ABSTRAK Eucheuma cottonii adalah salah satu tanaman sebagai sumber senyawa bioaktif yang menghasilkan berbagai macam metabolit sekunder yang dicirikan oleh spektum aktivitas biologis yang luas. Berbagai manfaat rumput laut yaitu sebagai bahan pangan, sebagai bahan industri farmasi dan industri makanan. Kandungan senyawa bioaktif pada Eucheuma cottonii sebagai metabolit sekunder adalah sebagai antibakteri, antioksida, antikoagulan. Beberapa masyarakat yang mengetahui tentang bakteri Escherichia coli sebagai penyebab penyakit infeksi saluran pencernaan, bakteri Escherichia coli adalah salah satu jenis spesies utama bakteri gram negatif. Tujuan dari penelitian ini adalah mempelajari pengaruh kosentrasi ektrak Eucheuma cottonii dalam etanol sebagai antibakteri terhadap pertumbuhan bakteri Escherichia coli. dan mempelajari daerah hambat maksimum ektrak Eucheuma cottonii sebagai antibakteri pada bakteri Escherichia coli. Penelitian ini dilakukan dengan menggunakan metode eksperimental dengan desain Rancangan Acak Lengkap (RAL). Menggunakan 4 perlakuan dan 6 kali ulangan. Dengan menggunakan media EMB dan BGLB untuk uji antibakteri. Hasil uji ANOVA menunjukkan terdapat perbedaan signifikan, setelah diberi perlakuan konsentrasi 0%,19,2%,35,7% dan 37,5% hasil dengan konsentrasi larutan 37,5% dengan rata-rata 3 mm, pada kosentrasi 37,5% sebagagai kosentrasi tertinggi tetapi masih katagori lemah. Kata kunci : Eucheuma cottonii, Antibakteri, Escherichia coli","PeriodicalId":346598,"journal":{"name":"Jurnal SAINS ALAMI (Known Nature)","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126079177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Susi Apriliani, Ahmad Syauqi, Ratna Djuniwati Lisminingsih
{"title":"Uji Ekstrak Buah Maja (Aegle marmelos) Sebagai Antibakteri Pada Bakteri Escherichia coli","authors":"Susi Apriliani, Ahmad Syauqi, Ratna Djuniwati Lisminingsih","doi":"10.33474/j.sa.v4i1.9907","DOIUrl":"https://doi.org/10.33474/j.sa.v4i1.9907","url":null,"abstract":"Maja fruit (Aegle marmelos) is a fruit that contains substances such as balm oil, 2-furocoumarins-psoralen and marmelosin (C13H12O). Maja fruit, roots and leaves have antibiotic properties. Plants that contain chemicals in the maja fruit can potentially act as antibacterials that inhibit bacterial growth. The bacteria that cause diarrhea are Coliform, Escherichia coli, Salmonella enterica and Shigella which are bacteria that cause food poisoning or gastrointestinal disorders. Chemical content of maja fruit (Aegle marmelos), namely alkaloids, terpenoids, coumarin, phenylpropanoids, tannins, polysaccharides and flavonoids. This study aims to determine the maja fruit extract has an anti-bacterial effect on minimum bacterial inhibitory growth and to determine the concentration of maja fruit extract on the minimum inhibitory growth of Escherechia coli bacteria. In this study, using the experimental method completely randomized design (CRD). Using 4 treatments and 6 repetitions. By using EMB and BGLB media. The mean of observations on the inhibitory growth test of Escherichia coli bacteria at a concentration of 19.2 was 0.43, a concentration of 35.7 was 0.48, at a concentration of 37.5 was 1.703 and a control was 0.33. Anova test results showed that there was a significant difference, after being treated with a concentration of 0%, 19.2%, 35.7% and 37.5%, the most effective results were with a solution concentration of 37.5% with an average of 1.703 mm.Keywords: Antibacterial, Maja Frutt, Escherechia coli ABSTRAK Buah maja (Aegle marmelos) adalah buah yang mengandung substansi seperti minyak balsem, 2-furocoumarins-psoralen dan marmelosin (C13H12O). Buah, akar dan daun maja mempunyai sifat antibiotik. Tumbuhan yang memiliki kandungan kimia pada buah maja dapat berpotensi sebagai antibakteri yang menghambat pertumbuhan bakteri. Bakteri yang menimbulkan diare adalah Coliform, Escherichia coli, Salmonella enterica, Shigella yang merupakan bakteri penyebab keracunan makanan atau gangguan saluran cerna. Kandungan kimia dari ekstrak buah maja yaitu alkaloid, terpenoid, kumarin, pherilpropanoid, tannin, polisakarida dan flavonoid. Tujuan penelitian ini untuk mengetahui ekstrak buah maja memiliki pengsruh antibakteri terhadap pertumbuhan hambat minimal bakteri Escherichia coli dan menentukan konsentrasi ekstrak buah maja terhadap pertumbuhan hambat minimal bakteri Escherichia coli. Dalam penelitian ini menggunakan metode eksperimental Rancangan Acak Lengkap (RAL). Menggunakan 4 perlakuan dan 6 kali ulangan. Dengan menggunakan media EMB dan BGLB. Rerata pengamatan pada pertumbuhan uji daya hambat bakteri pada konsentrasi 19,2 % yaitu 0,43 mm, konsentrasi 35,7% yaitu 0,48 mm, konsentrasi 37,5% yaitu I,70 mm dan pada kontrol 0,33 mm. Hasil uji ANOVA menunjukkan terdapat perbedaan signifikan, setelah diberi perlakuan konsentrasi 0%,19,2%,35,7% dan 37,5% hasil yang paling efektif yaitu dengan konsentrasi larutan 37,5% dengan rata-rata 1,703 mm. Kata kunci : Antibak","PeriodicalId":346598,"journal":{"name":"Jurnal SAINS ALAMI (Known Nature)","volume":"338 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124744442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}