Visnyk of Dnipropetrovsk University. Biology, medicine最新文献

{"title":"Protective/detoxicative function of metallothionein in the rat brain and blood induced by controlled cadmium doses","authors":"H. Shiyntum, G. A. Ushakovа","doi":"10.15421/021519","DOIUrl":"https://doi.org/10.15421/021519","url":null,"abstract":"Cadmium classified as a major carcinogen is considered a poisonous and unwanted heavy metal to a lot of tissues in many organism s . Of many publications already available , the general consensus is that the cadmium attenuating element is metallothionein (MT) through its interchangeable mechanism with Zn triggered by the presence of Cd, providing binding sites for Cd ions. MT was first discovered in the kidney cortex of the horse ; it represents a low molecular weight protein, rich in cysteine residues which effectively bind with metals. It s  functions consist in detoxification of heavy metals like mercury, arseni c, cadmium, homeostasis of essential metals including copper and zinc, anti-oxidation against reactive oxygen species, protection against DNA damage, oxidative stress, cell survival, angiogenesis, apoptosis, and increase of proliferation. In this work, we sought to highlight the protective function of MT in the brain and serum of rats by means of detoxification under induced effects of controlled Cd doses. We have done this by exposing Wistar rats to Cd at different doses in drinking water at different time intervals. In two independent experiments , 58 rats were subjected to 0.1 or 1.0 µg Cd 2+ /kg of body weight for 15 or 36 days under different conditions. The obtained data indicates the different functioning systems for the brain and the blood for MT metabolism under Cd effect. Our results indicate significant loss of metallothionein level in the brain and important increases in the amount of MT in serum proving that even minimal ingestion of toxic Cd is enough to trigger the release of MT protein in blood.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"5 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129675044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Radionuclide activity and the immune system functioning in residents of radiation contaminated areas","authors":"V. Sokolenko, S. Sokolenko","doi":"10.15421/021517","DOIUrl":"https://doi.org/10.15421/021517","url":null,"abstract":"The objective of this research is to assess the relation of radioactive contamination degree to immune system functioning, in the absence or presence of additional potential immunosuppressants. To achieve the objective, during the period of 1995–2015 we examined 250 people, students of Cherkasy State University, who lived in the areas of enhanced radiation monitoring before. Also we evaluated the additional impact of the emotional stress caused by examinations on examined students. Indicators of cellular immunity were determined by immunophenotyping and dyeing using Romanowsky-Giemsa method. The level of immunoglobulins in blood serum was determined by radial immunodiffusion (Mancini method). The level of cortisol in blood serum was determined by immunoenzyme method. We have found that in absence of the emotional stress among residents of the areas contaminated with radionuclides, cortisol level remained at the upper limit of homeostatic norm. There is an average positive correlation between the activity of radionuclides in the territories of residence and the level of cortisol. There are marked average positive correlations between the activity of radionuclides and the level of neutrophils, and low positive correlations with the levels of IgG and IgM in blood serum. Average negative correlations between the activity of radionuclides and the following parameters are also observed: absolute and relative number of functionally mature T-lymphocytes with phenotype CD3+, absolute and relative number of their helper subpopulation CD4+, absolute and relative number of natural killer cells with phenotype CD16+; and strong negative correlations with immunoregulatory index CD4+/CD8+. Cortisol level shows the similar correlation with the same parameters, but correlation coefficient is lower. Under conditions of additional stress, caused by emotional load during the examinations, cortisol level significantly increases. This enhanced previously discovered correlations and revealed new ones: average negative correlations between radionuclide activity / cortisol level and expression of pan-T cell marker CD5, medium positive correlations with absolute and relative number of suppressor T-lymphocytes with phenotype CD8+, correlation with the level of serum IgG changes from positive to negative. Additional short-term stress factor combined with chronic influence of low doses of radiation increases the dependence of immunosuppression, caused by radiation contamination of the areas where examined students live, and causes failures in the work of compensatory mechanisms, particularly at the level of serum immunoglobulin products.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130922183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Peculiarities of tissue water fractional composition in case of experimental whole-body hyperthermia","authors":"O. Kuznetsova","doi":"10.15421/021518","DOIUrl":"https://doi.org/10.15421/021518","url":null,"abstract":"The present study, using proton nuclear magnetic resonance relaxation (NMR) method, was undertaken to compare the water fractional composition in nature tissues (group 1) with those damaged by experimental whole-body hyperthermia (group 2). We measured longitudinal or “spin-lattice” (T 1 ) and transverse or “spin-spin” (T 2 ) relaxation times of protons of tissues (brain, the atria of the heart, the kidneys and the renal cortex) from adult Wistar rats. The differences in T 1 , T 2 and percentage of the intra- and extracellular water between group 1 and 2 were studied to help understand how the water moves in tissues at hyperthermia. The results of this study and the literature data allow to make conclusions about tissue water fractional composition in case of experimental whole-body hyperthermia: (1) fractional composition of water and the distribution of intra- and extracellular fluid in the tissue of the atria of the heart did not change (T 1 and T 2 relaxation times remained unchanged); (2) the crystalline water fraction increased in brain (longer T 1 relaxation rate and shorter T 2 relaxation rate). This is obstructing the exchange of protons between free and bound water in brain. Thus, loss of water by brain cells is prevented. The distribution between intra- and extracellular fluid in brain remained unchanged; (3) fraction of free water increased in renal tissue (simultaneous longer T 1 and T 2 relaxation rates) by reducing the volume of extracellular fluid; (4) thick hydration layer of water (longer T 1 relaxation rate, T 2 remained unchanged) was formed in the extracellular fluid of renal cortex. This water layer is formed around the sodium ions which concentration is increased in renal cortex tissue of rats from group 2. Аs a result, the amount of fluid secreted by kidneys is reduced, i.e. there is a retention of water in the body. The relevance of our research for the understanding of high temperatures’ adaptation mechanisms is discussed in this paper.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125769504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Helmintofauna of sheep and goats in Dnipropetrovsk region","authors":"A. A. Boyko","doi":"10.15421/021516","DOIUrl":"https://doi.org/10.15421/021516","url":null,"abstract":"Worms of classes Nematoda Rudolphi, 1808, Trematoda Rudolphi, 1808 and Cestoda Rudolphi, 1808 were registered in small ruminants (sheep and goats) in Dnipropetrovsk region. Identified nematode s belong to Strongylata Railliet et Henry, 1913, Rhabditata Chitwood, 1933 and Trichurata Skrjabin, 1915. Among the trematodes , representatives of Fasciolata (Skrjabin et Schulz, 1935) were identified. Cestodes were re presented by Anoplocephalata (Skrjabin, 1933). Among the specific types of nematodes , representatives of the Strongylata suborder were found : Muellerius sp. , Protostrongylus sp. , Haemonchus contortus Rundolphi , 1802, Nematodirus sp . Among the helminths Rhabditata we identified Strongyloides papillosus Wedl, 1856, Trichurata – Trichuris sp. , Fasciolata – Fasciola hepatica Linnaeus, 1758 and Dicrocelium lanceatum Stiles et Hassall, 1896, Anoplocephalata – Moniezia expansa (Rudolphi, 1810) and M. benedeni (Moniez, 1879). The dominant species among worms in ungulates was defined as nematode H. contortus . The smallest share in the group was taken by trematod e species F. hepatica and nematode s Trichuris sp. The highest biodiversity indices are recorded in samples of material selected from ungulates consuming pasture herbage from May to September (ten specie s of worms belonging to three classes : Muellerius sp. , Protostrongylus sp. , H. contortus , Nematodirus sp. , S. papillosus , Trichuris sp. , F. hepatica , D. lanceatum , M. expansa , M. benedeni were registered). In animals not pastured throughout the year three types of worms of the class Nematoda: S. papillosus , H. contortus and Nematodirus sp . were defined .","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116879352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Free and bound forms of sialic acid in blood plasma of patients with chronic lymphocytic leukemia","authors":"O. Netronina","doi":"10.15421/021520","DOIUrl":"https://doi.org/10.15421/021520","url":null,"abstract":"The level of free and bound forms of sialic acids (SА) was investigated in chronic lymphocytic leukemia (CLL) and at different stages of receiving chemotherapy. Determination of sialic acid in blood plasma of patients with CLL before treatment were carried out on the first day and 2 months after taking chemotherapy drugs for combination schemes. Hematologic ally healthy donors represented the control group. Determination of SA was conducted by thiobarbituric method using trichloroacetic acid for distribution of total sialic acids onto free , protein bound sialic acid, and oligo bound sialic acid. Level of free sialic acid in serum in c hronic lymphocytic leukemia was equal to 43.2% compared to the total number of plasma. On the first day of chemotherapy on background of the general level of oligo bound sialic acids we observed increase in free sialic acid by 24.2% compared to patients not receiving treatment. The level of sialic acids increased 2.6 times compared to norm and featured no significant change s at different stages of treatment. The concentration of sialic acids bound to proteins at 73.4% was lower compared to the control group. After receiving chemotherapy on the first day there was a decline of this indicator to 56.2% compared to groups of patients before treatment. Two months after the treatment the level of all parameters under study returned back to the values obtained at the start of treatment. Th is data can serve as an additional efficiency criterion of the chemotherapy.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125451790","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The dynamics of biofilm overgrowth of Enterococcus faecalis","authors":"E. A. Synetar, O. I. Brych","doi":"10.15421/021526","DOIUrl":"https://doi.org/10.15421/021526","url":null,"abstract":"The nature of microorganisms can exist in two physiological forms that allow microbes to preserve livelihoods and continue their life cycle. The first is the population of planktonic forms of microorganisms which live freely in the environment with the developed systems of active and passive mobility, contributing to the rapid spread of a liquid medium. The second forms are those express ing specific mechanisms of adhesion, and able to aggregate on biogenic and abiogenic surfaces. Even in the deep sea vast number of species of bacteria liv e in their inherent horizons. Thus, the study of biofilms tube life support systems, diagnostic, laparoscopic devices during prolonged catheterization of the urinary system is of great practical, theoretical and biological significance in medicine and biology. For a lmost 20% of catheter-associated infections antibiotic therapy is uneffective, particularly through the formation of microbial biofilms on the surface of urinary catheters. We characterized the dynamics of biofilm growth of Enterococcus faecalis on fragments of silicone catheter. The study was conducted using bacteriological and electron microscopic techniques. Study of the dynamics of biofilm formation was performed using E. faecalis strain 49, which is isolated from the urine of persons who are not the p atients of the urological department of resuscitation and intensive therapy. Using scanning electron microscopy we have established dynamics and phase attachment of E. faecalis bacteria and subsequent overgrowth of silicone catheter surface. After calculations , index of adhesion on the turbulent wall amounted to 0,49 microbial cells. Th at is, every other cell of the monolayer adhered on the catheter. Area of biofilm growth of E. faecalis after 24 hour incubation was equal to 51 . 5 μm 2 , in 48 hour s it increased to 231 .5 μm 2 . After 72 hours of incubation we recorded the increase in biofilm growth of E. faecalis to 1 922, 8 μm 2 . The results were obtained on fragments of catheters, immersed in broth in vertical position . This orientation has excluded the deposition of germs by sedimentation, i . e . by gravity. It is known that after the logarithmic phase and achieving M - concentration for a few hours microbes start to die and their possible deposition may occur . Therefore, our results confirm the formation of biofilm, instead of sediment ation of dead microbes. Our study show s that biofilm is “the way of overgrowth on artificial and natural surfaces by microorganisms that are kept on them by exopolymer membranes ” .","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"193 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124285445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of different correction methods of pyeloureteral segment according to the data of diuretic ultrasonography","authors":"Serìâ Bìologìâ, D. Vorobets","doi":"10.15421/021527","DOIUrl":"https://doi.org/10.15421/021527","url":null,"abstract":"Methods of estimation of effectiveness of the open and laparoscopic pyeloplasty, as well as endo-urological palliative methods – laser resection, balloon dilatation and endopyelotomy, which determine the anatomical and functional peculiarities of renal pelvis and pyelo-ureteral junction with the help of ultrasound diagnostics during the forced diuresis, have been proposed. Changes of the area of renal pelvis, the velocity of post-furosemide increase of the scope of renal pelvis, rate of its drainage, changes in the diameter of pyeloureteral junction have been studied. This methodical approach is non-invasive, informative and simple in application. It is shown that dispersions of samples of patients after the open surgery do not differ from the dispersions of samples of the same patients before the operation on such parameters as areas of renal pelvis before the induction of furosemide, areas of renal pelvis after 15 minutes administration of furosemide, the rate of drainage after furosemide, the original diameter of pyeloureteral junction. This may indicate the stability of surgery results. For example, the larger renal pelvis by kidney size before the operation corresponded to the larger designed pelvis after the operation; renal pelvis drained faster before the operation, features faster drainage after the operation as well. Variation in the areas of renal pelvis which decreased in 40 minutes after furosemide, percent rate of longitudinal pelvis area, rate of after-furosemide increase in pelvis area, diameter of pyeloureteral junction in 15 minutes administration of furosemide after the open pyeloplasty was significantly different compared to the variation in the same parameter for the same patients before the operations. More substantial difference was observed in the same patients before and after Anderson-Hynes surgery by parameters of relative rate of after-furosemide drainage of pelvis and increase in diameter of pyeloureteral junction after 15 min administration of furosemide. That is, the same principle of operation provides similar results by anatomical parameters, such as size and diameter of pyeloureteral junction, but quite different results by functional parameters which reflect the possibility of draining of kidney in forced diuresis. Successful open pyeloplasty leads to a significant decrease in the pelvis area at different time intervals after furosemide administration, the relative increase in the pelvis area on the background of the induction of diuresis, rate of pelvis drainage, increase (normalization) in diameter of pyeloureteral junction, including the larger (better) gap of pyeloureteral junction after administration of diuretic. Concerning laparoscopic pyeloplasty, the dispersion of mean values of S, SPR, Vpr, V, VOT, D, DD after the operation was significantly different from those before the operation. This means that as in the case with open surgery, satisfactory clinical results such as reduction in renal pelvis and re","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134300173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The patterns of utilization of sulfate and nitrate ions by bacteria Desulfomicrobium sp. CrR3 and Desulfovibrio desulfuricans Ya-11","authors":"L. Dorosh, T. Peretyatko, S. Gudz","doi":"10.15421/021528","DOIUrl":"https://doi.org/10.15421/021528","url":null,"abstract":"The aim of this work was to study the patterns of utilization of sulfate and nitrate ions by bacteria Desulfomicrobium sp. CrR3 and Desulfovibrio desulfuricans Ya-11 under different cultivation conditions. Chromium-resistant sulfate-reducing bacteria Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11 were used. Bacteria were grown in Posgate C medium at 30°C in 25 ml test tubes under anaerobic conditions. To test the ability of bacteria Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11 to use various substances and ions as electron acceptors, they were incubated in potassium phosphate buffer (10 mM, pH 7) with sulfate, nitrate and nitrite ions in concentrations of 1, 5 and 10 mM. At various concentrations of sulfate ions (1, 5 and 10 mM), biomass of bacteria Desulfomicrobium sp. CrR3 and D.  desulfuricans Ya-11 increased with the increase of concentration of electron acceptor, the maximum biomass was equal to 3.65 and 3.05 g/l at 10 mM of sulfate ions, respectively. With the increase of concentration of nitrate ions to 5 mM the biomass increased by 70% compared to the biomass of bacteria grown in the medium with nitrate ions at the concentration 1 mM. The maximal biomass was determined in the presence of nitrate ions at a concentration of 10 mM – 3.78 and 3.15 g/l for bacteria Desulfomicrobium sp. CrR3 and D.  desulfuricans Ya-11, respectively. It is found, as a result of incubation of bacteria Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11, that by introducing sulfate ions at a concentration of 5 mM bacteria Desulfomicrobium sp. CrR3 used 98%, while D. desulfuricans Ya-11 used only 86%, and under these conditions hydrogen sulfide has been detected in the incubation mixture at the concentration of 0.8–1.0 mM. In the presence of 10 mM of sulfate ions efficiency of electron acceptors utilization was equal to 85–95% for both strains. Bacteria Desulfomicrobium sp. CrR3 intensively used nitrate ions, the efficiency of electron acceptor utilization at 10 mM was equal to 92.8%, while for D. desulfuricans Ya-11 the usage percent amounted to 73% only, and nitrite ions were not observed after three days of incubation. It is established that bacteria Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11 are capable to use sulfate and nitrate ions as electron acceptors in the process of the disimilatory sulfate and nitratre reduction. As a result of the study of patterns of nitrate utilization by Desulfomicrobium sp. CrR3 and D. desulfuricans Ya-11 it is found that bacteria use nitrate as a nitrogen source for biosynthetic processes, and as electron acceptors. Under these conditions nitrates are reduced to nitrites, and then they are turned to ammonium.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127192364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relations between blood supply of brain of students and condition of autonomic nervous system and risk factors","authors":"L. Korovina, T. Zaporozhets","doi":"10.15421/021513","DOIUrl":"https://doi.org/10.15421/021513","url":null,"abstract":"The purpose of our research was to estimate the brain blood supply level by rheoencephalography method in junior students of the Medical academy and to determine the blood supply links with the autonomic regulation state, behavioural and alimentary factors. Rheo-encephalographic study, research of the autonomic nervous system state, heart rate regulation and questioning of 17–29 year-old students have been conducted. Basic hemodynamic indices were normal in all surveyed students. Increase in body weight index enhanced the probability of the brain blood supply deterioration. Adaptation mechanisms tension was accompanied by reduction of the rheographic index. Higher blood filling of the brain vessels corersponded to higher ratio “blood minute volume / due blood minute volume” defined taking into account the system arterial pressure. The quantity of links with indicators of the autonomic nervous system state was limited. Nonlinear dependence of the rheographic index on the Kerdo vegetative index was observed: the rheographic index value was the lowest in students with the autonomic balance by the Kerdo vegetative index; it was the highest in the group with the sympathetic prevalence. Risk factor of blood filling decrease was the reduction in the diet variety when foodstuffs of different groups were included into the diet less than twice a week, or they were excluded from the diet completely. Positive correlation of blood supply was observed more often with frequent consumption of fish, vegetables, and fresh fruits. Increase in the regular alcohol intake experience promoted decrease in brain blood supply and increase in asymmetries of blood supply indicators. The effect of alcohol was essential, despite young age of surveyed students and low level of alcohol consumption. Increase in the experience and intensity of smoking was accompanied by deterioration of brain blood supply indicators. Students with the best blood supply had the better academic progress. Observed multiple correlations of blood supply indicators with behavioural factors allowed to assert that reduction of risk factors (control of body weight, variety and full value of a diet, refusal of smoking, alcohol restriction even below doses which are considered safe) would allow to improve the brain blood supply.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132210590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered balance between proteolysis and antiproteolysis in oncohematologic diseases","authors":"O. E. Shaul’ska, L. Diachenko, T. P. Nikolayenko-Kamyshova, A. Shevtsova","doi":"10.15421/021512","DOIUrl":"https://doi.org/10.15421/021512","url":null,"abstract":"Досліджено активність трипсиноподібних ензимів, матриксних металопротеїназ ММП2 та ММП9, вміст α 1 -інгібітора протеїназ і α 2 -макроглобуліну у плазмі крові хворих із різними формами онкогематологічних захворювань до та після застосування цитостатичних препаратів доксорубіцину та даунорубіцину. Встановлено, що при проліферативних захворюваннях крові відбувається порушення балансу у системі протеоліз-антипротеоліз, що залежить від типу проліферувальних клітин і перебігу захворювання. У хворих із гострим мієлолейкозом і множинною мієломою концентрація інгібіторів підвищена на тлі збільшення активності трипсиноподібних ензимів та різноспрямованих змін проММП9. При хронічному лімфолейкозі вміст інгібіторів знижується на тлі нормальних значень активності трипсиноподібних ензимів і посилення активності ММП9. Під впливом антрациклінових антибіотиків цей баланс порушується за рахунок змін як активності протеолітичних ферментів, так і концентрації їх інгібіторів.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"2014 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121762730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}