Visnyk of Dnipropetrovsk University. Biology, medicine最新文献

{"title":"Genetic risk factors for Parkinson’s disease in Ukraine","authors":"A. Koliada, T. V. Pletneva, A. S. Sosedko, M. A. Chyvlyklyj, A. Vaiserman, I. Karaban","doi":"10.15421/021509","DOIUrl":"https://doi.org/10.15421/021509","url":null,"abstract":"The paper focuses on the genetic risk factors for Parkinson’s disease (PD) such as polymorphisms in genes CYP1A1, GSTM1 and APOE. A total number of 516 people were examined. 300 persons were in the control group (mean age 67,0 ± 0,4 years; 200 males and 100 females) and 216 persons were patients with PD (mean age 65,0 ± 0,7 years, 116 males and 100 females). Whole blood samples collected from each person were genotyped using PCR-RFLP. Amplification and restriction results were assessed by conducting vertical agarose gel electrophoresis. The study analyzed marker с.2452C>A in the CYP1A1 gene. In the control group, allele C frequency was 0.79, and allele A frequency – 0.21. Genotype frequencies were: CC – 0.61, AC – 0.36, AA – 0.03. In the group of patients alleles C and A frequencies were 0.64 and 0.36 correspondingly. Genotype frequencies were: CC – 0.35, AC – 0.58, AA – 0.07. There was a significant difference between both groups in allele A frequency. It is considered that 0/0 genotype for the GSTM1 gene is a risk factor for PD. In the controls, +/+ and 0/0 genotypes frequencies were 0.67 and 0.33 correspondingly. In the group of patients +/+ genotype frequency was 0.55 and 0/0 genotype frequency – 0.45. The difference was statistically significant. In the control group genotype frequencies for the АРОЕ gene were 0.715 (Е3/Е3), 0.077 (Е3/Е4), 0.009 (Е4/Е4), 0.167 (Е2/Е3), 0.031 (Е2/Е4) and 0.000 (Е2/Е2). In the group of patients with PD they were 0.634 (Е3/Е3), 0.148 (Е3/Е4), 0.032 (Е4/Е4), 0.157 (Е2/Е3), 0.023 (Е2/Е4) and 0.000 (Е2/Е2). Е3/Е4 genotype frequency was significantly higher in the group of patients with PD than in the control group. Pathogenic allele с.2452C>A of the CYP1A1 gene is associated with increased risk of PD (OR = 1.72). 0/0 genotype carriers have higher risk to develop PD (OR = 1.72). Allele έ4 of the АРОЕ gene may be associated with increased risk of PD. Risk of the disease is higher in έ2 allele carriers (OR = 2.35) and έ4 allele carriers (OR=1.97). People with genotype Е4/Е4 have chances to be affected by PD 3.48 times higher (OR = 3.48). Associations revealed in the different human populations between genetic factors and PD may vary that is associated with the genetic heterogeneity and proportion of environmental factors which affect people. Despite the results are sometimes controversial, they can be helpful in developing DNA-tests for early diagnosis of PD.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"315 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125316372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromatographic profiles of blood plasma free oligosaccharides in patients with cardiovascular disease","authors":"I. U. Pismenetskaya, T. Butters","doi":"10.15421/021510","DOIUrl":"https://doi.org/10.15421/021510","url":null,"abstract":"Free oligosaccharides (FOS) are unbound structural analogs of glycans of glycoconjugates. There are several sources of them inside the cell: 1) multistep pathways of N-glycosylation, 2) the cell quality control and endoplastic reticulum-associated degradation of mis-glycosylated and/or misfolded glycoproteins, 3) lysosomal degradation of mature glycoconjugates. Some of these FOS are the earliest indicators of potential glycosylation alterations that would be revealed in the course of the cell quality control and the endoplastic reticulum-associated degradation. Ischemia and hypertension cause stress of intracellular organelles leading to disruption of their functions. The main objective of the work was the characterization of free oligosaccharides (FOS) in plasma obtained from patients with cardiovascular diseases compared to those from healthy subjects to evaluate the potential of these compounds for diagnostics. Chromatographic profiles of FOS composed of 4–12 monosaccharides were obtained and analyzed for quantitative and qualitative differences between the samples. After plasma deproteinization and FOS purification the oligosaccharides were labelled with anthranilic acid (2-AA), separated into the neutral and charged with QAE Sephadex (Q25-120) chromatography and analysed using high-performance liquid chromatography (HPLC). Glucose unit values were determined following comparison with a 2-AA-labelled glucose oligomer ladder derived from a partial hydrolysate of dextran as an external standard. The data were collected and processed using Empower software. The charged FOS were digested with the sialidase from Arthrobacter ureafaciens . 2-AA – labelled free oligosaccharides from transferrin were used as an external standard for the structure decoding. The profiles obtained were compared with intracellular free oligosaccharides of known structures and with the glycan structures and their descriptions in the databases GlycoBase and EUROCarbDB. These approaches allowed predicting a range of glycan structures for each of the main peaks of HPLC profiles of plasma free oligosaccharides and managing ways for their future experimental analysis. In the case of cardiovascular disorders, HPLC profiles of FOS revealed a changing pattern of heterogeneity, depending on the severity of the disease. Three main enlarged glycan species in the netral fraction and one peak in the charged fraction distinguished the FOS of the patients from those of the healthy volunteers. It has been revealed that the neutral marker peaks were represented by polimannose glycans with 5–7 mannose residues and 1–2 residues of N-acetylglucosamine, and one of the major peaks of the charged fraction – by two-antennary complex N-glycan with two sialic acid residues. The study of free oligosaccharides of blood plasma is a new field of glycobiology allowing an evaluation of an organism state at the level of the cell organelle functional status and openning up broad prospects for finding earl","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115472728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological properties of aerococci and bacilli as a component of new associate-probiotic complex","authors":"S. I. Valchuk, D. Stepansky, T. N. Shevchenko, I. Koshevaya, S. A. Ryzhenko, G. N. Kremenchutsky","doi":"10.15421/021511","DOIUrl":"https://doi.org/10.15421/021511","url":null,"abstract":"Dysbioses of the gastrointestinal tract are common among people of all ages and genders. Development of this pathology is associated with a number of complications, from indigestion to occurrence of malignant disease. Therefore, there is a need in development of measures of their prevention and correction. Probiotics are used as drugs against dysbiosis. Most of the presently known probiotics contain bacterial cells of one species, although combination preparations feature higher efficiency. At the same time, there are difficulties in construction of these drugs, primarily due to incompatibility of physiological properties of microorganisms and mutually antagonistic action of their components. The aim was to examine the compatibility of Bacillus subtilis and Aerococcus viridans in a single preparation, their antagonistic activity against different strains of test-cultures and general antagonism directed on different groups of bacteria for subsequent formation of associative probiotic complex. Properties of aerococci strains were studied and A. viridans 167 strain was selected for inclusion into the probiotic preparation. The tested strain showed the highest indicators of production of hydrogen peroxide, which is one of the mechanisms of antagonistic effect against opportunistic pathogens. General study of biological properties of aerococci strains showed that producing of hydrogen peroxide and superoxide radical in them was conditioned by functioning of NAD-independent lactatoxidase. It has been determined that antioxidant defense of aerococci from the action of endogenous and active excretable forms of oxygen was provided by activity of superoxide-dismutase and GSH-peroxidase. The method of deferred antagonism found no depressing mutual action between probiotic strains of B. subtilis 3 and A. viridans 167 at their joint cultivation. Inhibition of growth at the joint application of A. viridans 167 and B. subtilis 3 strains was recorded for both museum and clinical strains of test-cultures Escherichia coli , Proteus vulgaris , Klebsiella ozaenae , Citrobacter freundii , Pseudomonas aeruginosa , Staphylococcus aureus , S. epidermidis , Candida albicans . Separate application of A. viridans 167 or B. subtilis 3 against strains of these opportunistic pathogens was characterized by relatively less antagonistic effect of each of strains under study. The results allow us to recommend the studied strains of B. subtilis 3 and A. viridans 167 for use as the components to construct a new associative probiotic preparation.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"167 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131436896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of immunomodulators of natural origin on cellular immunity indices in blood of broiler chicken under stress","authors":"S. Grabovskyi, O. Grabovska","doi":"10.15421/021507","DOIUrl":"https://doi.org/10.15421/021507","url":null,"abstract":"The paper deals with researching of T- and B-lymphocytes relative quantity and functional activity in broiler chicken blood after using of animal origin immunomodulators in conditions of pre-slaughter stress. The authors determined the relative amount of T- and B-lymphocytes and their individual populations in the reaction of spontaneous rоsetting with the sheep erythrocytes in blood. Besides, the differentiated count of rоsetting lymphocytes with the various degree of functional activity was conducted. The spleen extract (70% alcohol solution in volume of 1.4 ml per chicken) was added to the diet of broiler chicken of experimental groups by aerosol method. This extract was obtained with/ without ultrasound application. 70% alcohol solution in the same volume and using the same method was added to the diet of broiler chicken of the control group five days before slaughter. The authors have not established probable increase of T-lymphocytes general quantity in broiler chicken blood in both experimental groups. It is shown that pre-slaughter stress in broiler chicken caused by weaning has immuno-suppressive effect on T- and B-lymphocytes in blood, which is accompanied by their quantity and functional activity decrease. T- and B-lymphocytes amount and functional activity of T- and B-cell immunity was stimulated after adding immunomodulators of natural origin to broiler chicken diet. Spleen extract polyamines as immunomodulators and antistressors most effectively influenced on some of cell immunity indices before slaughter – it is necessary to note the increase in T-helper lymphocytes in the broiler chickens blood caused by lymphocytes with medium (6–10) – by 18% (Р < 0.05) and high density receptors (М) – by 35% (Р < 0.05) compared to the control. It is shown that decrease of T-lymphocytes quantity in broiler chicken blood is caused by lymphocytes with law (3–5) – by 22% (Р < 0.01) and high (M) – by 11% (Р < 0.05) density receptors with increase of medium density receptors (6–10) – by 15% (Р < 0.05) compared to the control. The results obtained in the experiment can be used in the investigations of cell immunity indices of farm animals in order to increase the organism resistance and to correct their pre-slaughter stress.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115042120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dissimilatory sulfate reduction in bacteria Desulfovibrio desulfuricans ІМV К-6 upon influence of Uragan and Raundup herbicides","authors":"G. Zvir, O. Moroz, S. Hnatush","doi":"10.15421/021508","DOIUrl":"https://doi.org/10.15421/021508","url":null,"abstract":"З’ясовано здатність сульфатвідновлювальних бактерій Desulfovibrio desulfuricans ІМВ К-6 рости та відновлювати сульфат-іони до гідроген сульфіду за впливу гербіцидів Ураган і Раундап. Нагромадження біомаси бактеріями у контролі та за впливу гербіцидів було найвищим на четверту – шосту добу культивування, після чого бактерії переходили у стаціонарну фазу росту. За впливу гербіцидів біомаса D. desulfuricans ІМВ К-6 була вищою порівняно з контролем. Нагромадження біомаси змінювалося відповідно до зростання концентрації гербіцидів у середовищі. Сульфатвідновлювальні бактерії D. desulfuricans ІМВ К-6 за наявності сульфатів та органічних сполук у середовищі здійснюють відновлення сульфат-іонів до гідроген сульфіду (дисиміляційна сульфатредукція). У контрольному середовищі процес відновлення сульфат-іонів бактеріями D. desulfuricans ІМВ К-6 був найінтенсивнішим упродовж перших чотирьох діб. За цих умов у середовищі виявлено максимальну концентрацію гідроген сульфіду. Внесення гербіцидів у середовище культивування стимулювало процес відновлення сульфат-іонів і утворення гідроген сульфіду сульфатвідновлювальними бактеріями D. desulfuricans ІМВ К-6. Здатність D. desulfuricans ІМВ К-6 нагромаджувати вищі рівні біомаси та здійснювати дисиміляційну сульфатредукцію за впливу Урагану та Раундапу може бути зумовлена наявністю у цих гербіцидах невисоких концентрацій інертних компонентів (сульфатів), що можуть бути використані бактеріями як акцептори електронів у сульфатному диханні.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129035613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improvement of the method of obtaining human IgA Fc-fragments","authors":"O. Galkin, Y. Gorshunov, V. F. Solovjova","doi":"10.15421/021506","DOIUrl":"https://doi.org/10.15421/021506","url":null,"abstract":"To address a number of fundamental and applied problems in immunology, molecular and cellular biology and biotechnology it is necessary to obtain Fc-fragments of immunoglobulins. Fc-fragments may be used for studying of the effector functions of antibodies which are mediated by these areas. They are often used as an immunogen to produce anti-specie (based on so-called secondary antibody) conjugate in the development of serological tests for diagnostics (predominantly such conjugate based on monoclonal antibodies). The work is aimed to develop improved methods of obtaining and allocation of Fc-fragments of human IgA. To achieve this objective, optimization of hydrolysis of IgA with subsequent purification of Fс-fragments have been carried out. Improved method of obtaining Fc-fragments of IgA provides: papain hydrolysis of immunoglobulin in the environment of nitrogen for 4 h, allowing to achieve maximum output of Fc-fragments without their further degradation: isolation and purification of Fc-fragments of human IgA by one-stage gel filtration on sephadex G-100; control of purity of the target product by electrophoresis in polyacrylamide gel with sodium dodecyl sulfate and Ouchterlony immunodiffusion. Enzymatic hydrolysis was carried out at the optimal temperature of papain (37 °C). As the oxygen in the air may have inhibitory effect on enzymatic hydrolysis reaction, the reaction mixture was incubated in the nitrogen atmosphere to prevent inactivation of papain. To reduce the incident degradation of immunoglobulin molecules, papain hydrolysis was carried out without using an enzyme activator (cysteine). Usage of the proposed scheme allows obtaining Fc-fragments of human IgA of high purity. Outcome of Fc-fragments after all stages of purification was about 18% of the initial amount of IgA in the preparation. Molecular weight from Fc-fragments of human IgA was equal to approximately 70 kDa.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"408 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122861046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of fibronectin under conditions of doxorubicin action","authors":"A. Shevtsova, G. Ushakova","doi":"10.15421/021503","DOIUrl":"https://doi.org/10.15421/021503","url":null,"abstract":"There is no standard as to treatment of anthracycline chemotherapy complications. The reduction of cytotoxic drugs toxicity without weakening of their antitumor action remains relevant. The extracellular matrix which key component is fibronectin is present in all tissues and it continuously undergoes controlled remodeling. So , the purpose of our work was to study the level of fibronectin in the experimental model of doxorubicin-induce d cardiomyopathy and effects of this cytostatic and its co-administration with antioxidants of different nature. The level of fibronectin was measured by ELISA using monospecific antibodies against fibronectin (Sigma, USA), secondary anti-IgG labeled with horseradish peroxidase (Sigma, USA) and fibronectin standard (Sigma, USA). The study was conducted on Wistar male rats with weight of 210 ± 50 g which were divided into 4 groups by 8 animals in each group: 1 – control, rats receiv ing saline i/p ; 2 – doxorubicin 1 mg/kg i/p once a week during 4 weeks ; 3 – doxorubicin by the same scheme plus 1% 2-oxoglutarate in drinking water during 4 weeks; 4 – doxorubicin by the same scheme and korvitin injection 30 min before doxorubicin appl ication once a week during 4 weeks. Obtained data indicate the effect of doxorubicin to decrease in i ndex mass heart i n 38% of animals compared to control animals; decrease in total protein concentration by 8% ( Р < 0,05 ) and increase of the level of fibronectin by 6 7 % ( P < 0,00 1) in blood plasma of rats and decrease in the level of fibronectin in the heart extract by 19% ( Р < 0,0 5) under development of doxorubicin-induced cardiotoxicity. Increased fibronectin concentration in blood plasma had strong correlation with decreased total protein concentration in blood (r = 0,8 0 ) and heart extract (r = 0,59) in rats with doxorubicin-induced cardiomiophaty indicating the sens i tive reaction of fibronectin to development of metabolic disorders under doxorubicin influence.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125318616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutathione metabolism system under condition of transplantation of muscle tissue in rats","authors":"O. V. Kulibaba, S. M. Kozishkurt, O. Duzenko, I. Vovchuk, S. Petrov","doi":"10.15421/021504","DOIUrl":"https://doi.org/10.15421/021504","url":null,"abstract":"Наведено результати визначення кількості відновленого глутатіону, активності глутатіонредуктази та глутатіонпероксидази після трансплантації м’язових тканин, узятих у щурів з одного посліду. Проведено два види операційного втручання: трансплантацію м’язових тканин, узятих з одного посліду у щурів, та операцію без підсадки. Трансплантацію проводили на білих нелінійних щурах-самцях масою 180–300 г. Тварин виводили з експерименту шляхом пропускання електричного струму через довгастий мозок. Для отримання донорів для трансплантації використані самці з одного посліду. У дорослих щурів-донорів витягали черевну м’язову тканину, яку підшивали до гомологічної тканини щура-реципієнта. Таку ж процедуру проводили і зі стегновою м’язовою тканиною. Операцію без підсадки здійснювали для порівняння впливу змін досліджуваних показників за хірургічного впливу. Контролем слугувала тканина, яка не підлягала ніяким хірургічним втручанням. Досліджувані показники визначали на першу, третю та сьому добу після трансплантації. Трансплантація м’язової тканини, взятої у тварин з одного посліду викликає достовірне зменшення кількості відновленого глутатіону у стегновій м’язовій тканині реципієнта та в черевній м’язовій тканині донора та реципієнта і достовірне збільшення активності глутатіонредуктази у стегновій м’язовій тканині донора та в черевній м’язовій тканині донора та реципієнта.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133246425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of transplantation of muscle tissue in rats from the same litter on total number of flavins and FAD","authors":"S. Kobylnik, I. Vovchuk, O. O. Dosenko, S. N. Kozishkurt, D. V. Morosova, S. Petrov","doi":"10.15421/021505","DOIUrl":"https://doi.org/10.15421/021505","url":null,"abstract":"Riboflavin is a member of redox enzymes involved in fatty acid oxidation and energy generation. Important role of this vitamin is in reproductive function. Exchange of transformation of riboflavin in animal tissues and cells of microorganisms include reactions that lead to synthesis and subsequent collapse of FMN and FAD. It is involved in enhancing antitumor activity of many anticancer drugs, as well as activation of the immune system to kill tumor cells. Issues of transport of riboflavin and its derivatives in animals have been studied enough. Investigations of changes of the balance of riboflavin and its metabolites in muscular tissues before transplantation in rats from one litter and at operation without replanting were conducted, based on the Udenfriend method of flavin determination. Transplantation in the experiment was carried out on white non-linear male rats weighing 180–300 g. Animals were taken out of the experiment by passing electric current through the medulla. Belly muscular tissue was taken from donor rats of the same litter, and that tissue was sewn to homological muscular tissue of the recipient. The same procedure was carried out with femoral muscular tissue. In the course of operation without replanting the same manipulations have been made except for transplantation stage (for determination of the effect of surgical intervention). Tissue not subject to any surgical intervention served as a control. Parameters of the study were measured on the first, third and seventh days after transplantation. Transplantation of muscular tissue caused no changes in total flavin amount. Content of RF + FMN after transplantation of muscular tissue in rats of the same litter decreased in femoral muscular tissue of the recipient. Transplantation of muscular tissues in rats from the same litter lead to increase in FAD amount in femoral muscular tissue of the donor and recipient on the third day of the experiment. Transplantation of femoral muscular tissue lead to acceleration of FAD synthesis from riboflavin and FMN.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"29 3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123327720","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural trace of adaptation in motive nuclei of spinal cord of rats in hypokinesia and after physical loading in the recovery period","authors":"S. Popel, B. M. Mitckan, S. Z. Krasnopolsky, I. Melnik","doi":"10.15421/021502","DOIUrl":"https://doi.org/10.15421/021502","url":null,"abstract":"The purpose of this paper is to study the morphological changes of neurocytes in spinal cord of rats in hypokinesia and subsequent physical loading. Studies were performed on 55 laboratory rats of Wistar line. Materials of the research were the anterior horns of the gray matter of L5-S2 spinal segments. Preparations stained by Nissl and Viktorov were examined histologically. Hypokinesia was modeled following on the author’s technique. It was established that during prolonged hypokinesia in neurocytes of spinal cord of rats morphological changes in cell size and shape of the motor nuclei of all segments under study have been recorded. The number of hypochromic, hyperchromic destructively unchanged and hyperchromic destructively altered neurocytes increase; shadow cells appears, as well as cases of satellitosis and neuronophagia. Decrease in of albumen synthetical neurocyte function has been recorded. Physical loading of the average aerobic capacity leads to normalization of structural and functional state of neurocytes and enhances the reparative processes, as evidenced by a number of positive changes in morphometric parameters: increase in the number of normochromic neurocytes and decreasing the number of hyper- and hypochromic neurocytes with destructive signs, absence of pyknotic forms. Morphological parameters of neurocytes and their nuclei after physical loading of average aerobic capacity do not differ from those in the control group of intact animals. In neurocytes of this group of rats RNA concentration increases by 12.6% compared to animals after prolonged hypokinesia. Neurocytes of spinal cord of rats after prolonged hypokinesia develop significant morphological changes which are characterized by emergence of a significant number of hyperchromic neurocytes with signs of destructive changes and shadow cells, as well as and hypochromic neurocytes with signs of destructive changes, reduction in size and change of shape of perikaryons of neurocytes and their nuclei. Morphological changes of neurocytes after prolonged hypokinesia are accompanied by violations of biosynthetic processes, as evidenced by RNA decrease in the cytoplasm of efferent neurocytes of spinal cord of rats. Physical loading of average aerobic capacity leads to normalization of structural and functional state of neurocytes and promotes the reparative processes suported by positive changes in morphometric parameters.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127624576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}