BioTech最新文献

{"title":"Composition, Seasonal Dynamics and Metabolic Potential of the Rhizosphere Microbiome Associated with Wild White Poplar.","authors":"Mikhail I Popchenko, Dmitry S Karpov, Natalya S Gladysh, Maxim A Kovalev, Vsevolod V Volodin, George S Krasnov, Alina S Bogdanova, Nadezhda L Bolsheva, Maria S Fedorova, Anna V Kudryavtseva","doi":"10.3390/biotech13040052","DOIUrl":"10.3390/biotech13040052","url":null,"abstract":"<p><p>The white poplar (<i>Populus alba</i>) is a dioecious woody plant with significant potential for the phytoremediation of soils. To realize this potential, it is necessary to utilize growth-promoting microorganisms. One potential source of such beneficial microorganisms is the rhizosphere community of wild-growing trees. However, the structure, dynamics, and metabolism of the rhizosphere community of wild-growing white poplar remain poorly understood. To ascertain seasonal dynamics, species diversity, and metabolic potential, we sequenced 16S rRNA genes in metagenomes derived from 165 soil samples collected in spring and autumn from the root surfaces of 102 trees situated in disparate geographical locations. The three most prevalent phyla across all samples are Proteobacteria, Actinobacteriota, and Acidobacteriota. At the order level, the most prevalent orders are Sphingomonadales and Rhizobiales. Accordingly, the families Sphingomonadaceae and Rhizobiaceae were identified as dominant. The rhizospheric microbiome exhibited substantial inter-seasonal variation. Six families, including Caulobacteraceae, Xanthomonadaceae, Chitinophagaceae, Chthoniobacteraceae, Sphingomonadaceae, and Rhizobiaceae, exhibited alterations (spring-to-autumn) across all geographical locations under study. Members of the Rhizobiaceae family, which includes nitrogen-fixing bacteria, can provide poplar with plant-available forms of nitrogen such as nitrate and ammonium. The rhizosphere microbiome may facilitate the conversion of inorganic sulfur into sulfur-containing amino acids, cysteine and methionine, that are bioavailable to plants. Furthermore, the rhizosphere microbiome is capable of synthesizing amino acids, organic acids (including Krebs cycle acids), and some lipids and sugars. Consequently, the rhizosphere community can stimulate poplar growth by providing it with readily available forms of nitrogen and sulfur, as well as building blocks for the synthesis of proteins, nucleic acids, and other macromolecules. Many of these pathways, including nitrogen fixation, were subjected to seasonal changes.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11674042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142898703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Biotechnological Potential of Crickets as a Sustainable Protein Source for Fishmeal Replacement in Aquafeed.","authors":"Aldo Fraijo-Valenzuela, Joe Luis Arias-Moscoso, Oscar Daniel García-Pérez, Libia Zulema Rodriguez-Anaya, Jose Reyes Gonzalez-Galaviz","doi":"10.3390/biotech13040051","DOIUrl":"10.3390/biotech13040051","url":null,"abstract":"<p><p>As aquaculture production grows, so does the demand for quality and cost-effective protein sources. The cost of fishmeal (FM) has increased over the years, leading to increased production costs for formulated aquafeed. Soybean meal (SBM) is commonly used as an FM replacer in aquafeed, but anti-nutritional factors could affect the growth, nutrition, and health of aquatic organisms. Cricket meal (CM) is an alternative source with a nutrient profile comparable to FM due to its high protein content, digestibility, and amino acid profile. CM use in aquafeed influences growth and reproductive performance while modulating the gut microbiota and immune response of fish and shrimp. However, consistent regulation and scaling up are necessary for competitive prices and the marketing of CM. Moreover, the chitin content in CM could be an issue in some fish species; however, different strategies based on food biotechnology can improve the protein quality for its safe use in aquafeed.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11587023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Climate Resilience in Farm Animals: Transcriptomics-Based Alterations in Differentially Expressed Genes and Stress Pathways.","authors":"Chikamagalore Gopalakrishna Shashank, Veerasamy Sejian, Mullakkalparambil Velayudhan Silpa, Chinnasamy Devaraj, Aradotlu Parameshwarappa Madhusoodan, Ebenezer Binuni Rebez, Gajendirane Kalaignazhal, Artabandhu Sahoo, Frank Rowland Dunshea","doi":"10.3390/biotech13040049","DOIUrl":"10.3390/biotech13040049","url":null,"abstract":"<p><p>The livestock sector, essential for maintaining food supply and security, encounters numerous obstacles as a result of climate change. Rising global populations exacerbate competition for natural resources, affecting feed quality and availability, heightening livestock disease risks, increasing heat stress, and contributing to biodiversity loss. Although various management and dietary interventions exist to alleviate these impacts, they often offer only short-lived solutions. We must take a more comprehensive approach to understanding how animals adapt to and endure their environments. One such approach is quantifying transcriptomes under different environments, which can uncover underlying pathways essential for livestock adaptation. This review explores the progress and techniques in studies that apply gene expression analysis to livestock production systems, focusing on their adaptation to climate change. We also attempt to identify various biomarkers and transcriptomic differences between species and pure/crossbred animals. Looking ahead, integrating emerging technologies such as spatialomics could further accelerate genetic improvements, enabling more thermoresilient and productive livestock in response to future climate fluctuations. Ultimately, insights from these studies will help optimize livestock production systems by identifying thermoresilient/desired animals for use in precise breeding programs to counter climate change.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586948/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging Walnut Somatic Embryos as a Biomanufacturing Platform for Recombinant Proteins and Metabolites.","authors":"Paulo A Zaini, Katherine R Haddad, Noah G Feinberg, Yakir Ophir, Somen Nandi, Karen A McDonald, Abhaya M Dandekar","doi":"10.3390/biotech13040050","DOIUrl":"10.3390/biotech13040050","url":null,"abstract":"<p><p>Biomanufacturing enables novel sources of compounds with constant demand, such as food coloring and preservatives, as well as new compounds with peak demand, such as diagnostics and vaccines. The COVID-19 pandemic has highlighted the need for alternative sources of research materials, thrusting research on diversification of biomanufacturing platforms. Here, we show initial results exploring the walnut somatic embryogenic system expressing the recombinant receptor binding domain (RBD) and ectodomain of the spike protein (Spike) from the SARS-CoV-2 virus. Stably transformed walnut embryo lines were selected and propagated in vitro. Both recombinant proteins were detected at 3-14 µg/g dry weight of tissue culture material. Although higher yields of recombinant protein have been obtained using more conventional biomanufacturing platforms, we also report on the production of the red pigment betanin in somatic embryos, reaching yields of 650 mg/g, even higher than red beet <i>Beta vulgaris</i>. This first iteration shows the potential of biomanufacturing using somatic walnut embryos that can now be further optimized for different applications sourcing specialized proteins and metabolites.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586998/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Honey Bioactive Molecules: There Is a World Beyond the Sugars.","authors":"Gregorio Bonsignore, Simona Martinotti, Elia Ranzato","doi":"10.3390/biotech13040047","DOIUrl":"10.3390/biotech13040047","url":null,"abstract":"<p><p>Honey's many bioactive compounds have been utilized historically to cure infectious diseases. Beneficial effects are its antiviral, antibacterial, anti-inflammatory, antioxidant, and immune-stimulating qualities. The bee species, geographic location, botanical origin, harvest season, processing, and storage conditions all affect honey's potential for therapeutic use. Honey contains a number of antioxidants and active compounds, such as polyphenols, which have been shown to have disease-preventive properties. Based on their origins, categories, and functions, the main polyphenols found in various honey varieties are examined in this review.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11587060/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-Resolution Melting Analysis Potential for <i>Saccharomyces cerevisiae</i> var. <i>boulardii</i> Authentication in Probiotic-Enriched Food Matrices.","authors":"Monika Borkowska, Michał Kułakowski, Kamila Myszka","doi":"10.3390/biotech13040048","DOIUrl":"10.3390/biotech13040048","url":null,"abstract":"<p><p>To date, the only probiotic yeast with evidence of health-promoting effects is <i>Saccharomyces cerevisiae</i> var. <i>boulardii</i>. The expanded market including dietary supplements and functional foods supplemented with <i>Saccharomyces cerevisiae</i> var. <i>boulardii</i> creates an environment conductive to food adulterations, necessitating rapid testing to verify product probiotic status. Herein, qPCR-HRM analysis was tested for probiotic yeast identification. The effectiveness of the primer pairs' set was examined, designed to amplify heterogeneous regions in (a) rDNA sequences previously designed to identify food-derived yeast and (b) genes associated with physiological and genotypic divergence of <i>Saccharomyces cerevisiae</i> var. <i>boulardii.</i> Preliminary tests of amplicons' differentiation power enabled the selection of interspecies sequences for <i>18SrRNA</i> and ITS and genus-specific sequences <i>HO</i>, <i>RPB2</i>, <i>HXT9</i> and <i>MAL11.</i> The multi-fragment qPCR-HRM analysis was sufficient for culture-dependent <i>Saccharomyces cerevisiae</i> var. <i>boulardii</i> identification and proved effective in the authentication of dietary supplements' probiotic composition. The identification of <i>S. cerevisiae</i> var. <i>boulardii</i> in complex microbial mixtures of kefir succeeded with more specific intragenus sequences <i>HO</i> and <i>RPB2.</i> The predominance of <i>S. cerevisiae</i> var. <i>boulardii</i> in the tested matrices, quantitatively corresponded to the probiotic-enriched food, was crucial for identification with qPCR-HRM analysis. Considering the reported assumptions, qPCR-HRM analysis is an appropriate tool for verifying probiotic-enriched food.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586983/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and Characterization of Sialic Acid-Binding Variable Lymphocyte Receptors from Hagfish.","authors":"Mark Rickard N Angelia, Abigail Joy D Rodelas-Angelia, Cheolung Yang, Sojeong Park, Seung Pyo Jeong, Hyeok Jang, Dennis Berbulla Bela-Ong, Hobin Jang, Kim D Thompson, Taesung Jung","doi":"10.3390/biotech13040046","DOIUrl":"10.3390/biotech13040046","url":null,"abstract":"<p><p>Sialic acid is a diverse group of monosaccharides often found on the termini of <i>N</i>- and <i>O</i>-linked glycans as well as being components of glycoconjugates. Hypersialylation has been associated with the progression of chronic inflammation-mediated diseases such as cardiovascular disease and cancer. Given its role in infection and disease-related processes, sialic acid is a promising target for therapeutic approaches that utilize carbohydrate-binding molecules. In this study, we screened for sialic acid-recognizing variable lymphocyte receptors (VLRBs) or ccombodies from inshore hagfish (<i>Eptatretus burgeri</i>) using a synthetic Neu5Ac-glycoconjugate as an antigen in immunoassay. Resulting ccombodies, 2D8, 5G11, 4A1, and 5F8 were further characterized in terms of their binding activity and specificity. A competitive ELISA using free haptens showed strong inhibition using either <i>N</i>-acetylneuraminic acid (Neu5Ac) and <i>N</i>-glycolylneuraminic acid (Neu5Gc). The half-maximal inhibitory concentrations (IC₅₀) for Neu5Ac ranged from 7.02 to 17.06 mM, with candidates 4A1 and 5G11 requiring the least and highest amounts, respectively. IC₅₀ values for Neu5Gc ranged from 8.12 to 13.91 mM, for 4A1 and 5G11, respectively. Candidate ccombodies also detected naturally occurring sialic acid from known sialoglycoproteins using a dot blot assay. Neu5Gc-5G11 and Neu5Ac-2D8 yielded the strongest and weakest docking interactions with affinity values of -5.9 kcal/mol and -4.9 kcal/mol, respectively. Hydrogen bonding and hydrophobic interactions were predicted to be the predominant noncovalent forces observed between the ccombodies and sialic acid. This study demonstrates that glycan-binding VLRBs from hagfish hold promise in augmenting the glycobiologists' toolkit in investigating the roles of glycans in human and animal health and disease.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586995/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Insemination as a Possible Convenient Tool to Acquire Genome-Edited Mice via In Vivo Fertilization with Engineered Sperm.","authors":"Masahiro Sato, Emi Inada, Issei Saitoh, Kazunori Morohoshi, Shingo Nakamura","doi":"10.3390/biotech13040045","DOIUrl":"10.3390/biotech13040045","url":null,"abstract":"<p><p>Advances in genome editing technology have made it possible to create genome-edited (GE) animals, which are useful for identifying isolated genes and producing models of human diseases within a short period of time. The production of GE animals mainly relies on the gene manipulation of pre-implantation embryos, such as fertilized eggs and two-cell embryos, which can usually be achieved by the microinjection of nucleic acids, electroporation in the presence of nucleic acids, or infection with viral vectors, such as adeno-associated viruses. In contrast, GE animals can theoretically be generated by fertilizing ovulated oocytes with GE sperm. However, there are only a few reports showing the successful production of GE animals using GE sperm. Artificial insemination (AI) is an assisted reproduction technology based on the introduction of isolated sperm into the female reproductive tract, such as the uterine horn or oviductal lumen, for the in vivo fertilization of ovulated oocytes. This approach is simpler than the in vitro fertilization-based production of offspring, as the latter always requires an egg transfer to recipient females, which is labor-intensive and time-consuming. In this review, we summarize the various methods for AI reported so far, the history of sperm-mediated gene transfer, a technology to produce genetically engineered animals through in vivo fertilization with sperm carrying exogenous DNA, and finally describe the possibility of AI-mediated creation of GE animals using GE sperm.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11587059/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultraviolet, Did the Cell See It from the Side or the Bottom? Assessment and Modeling of UV Effects on Cultured Cells Using the CL-1000 UV-Crosslinker.","authors":"Takahiro Oyama, Kai Yanagihara, Anna Arai, Takanori Kamiya, Midori Oyama, Takashi Tanikawa, Takehiko Abe, Tomomi Hatanaka","doi":"10.3390/biotech13040044","DOIUrl":"10.3390/biotech13040044","url":null,"abstract":"<p><p>Numerous natural extracts and compounds have been evaluated for their ability to mitigate the adverse effects of ultraviolet (UV) overexposure. However, variability in the UV doses that trigger biological responses across studies likely arises from inconsistencies in UV exposure standardization. We hypothesize that these discrepancies are due to variations in culture plates and dishes. The UV dose (D) required to reduce cell viability by 50% differed by a factor of ten between 3.5 cm dishes and 96-well plates. Similarly, the EC₅₀ dose for IL-6 release (<i>D</i>_1/2) varied, potentially correlating with the surface area (S). UV exposure to wells with increasing height in 3.5 cm dishes resulted in a decrease in IL-6 release, suggesting that the greater the well height, the more it may influence UV exposure through reflection or shielding effects, thereby contributing to the physiological effects on the cells. To compare these differences among plates, we defined the height-to-diameter ratio (r). Analysis revealed a linear correlation between <i>D</i>_1/2 and S in a log-log plot, and between <i>D</i>_1/2 and r in a semi-log plot. From this, we defined two empirical indices <i>σ</i> and ρ for UV dose adjustment. A deductive model was also developed to derive a D' value that adjusts UV doses without requiring training. As with σ and ρ, the UV dose D was effectively adjusted using D' as well. These attempts suggest that D' offers a foundational framework for evaluating UVB effects on cultured cells.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11587097/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142711275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbial Protein and Metabolite Profiles of <i>Klebsiella oxytoca M5A1</i> in a Bubble Column Bioreactor.","authors":"Tawakalt Ayodele, Musiliu Liadi, Abodunrin Tirmidhi Tijani, Kudirat Alarape, Christiana Bitrus, Clairmont L Clementson, Ademola Hammed","doi":"10.3390/biotech13040043","DOIUrl":"https://doi.org/10.3390/biotech13040043","url":null,"abstract":"<p><p>The production of microbial proteins (MPs) has emerged as a critical focus in biotechnology, driven by the need for sustainable and scalable alternatives to traditional protein sources. This study investigates the efficacy of two experimental setups in producing MPs using the nitrogen-fixing bacterium <i>Klebsiella oxytoca M5A1</i>. <i>K. oxytoca M5A1</i>, known for its facultative anaerobic growth and capability to fix atmospheric nitrogen, offers a promising avenue for environmentally friendly protein production. This research compares the performance of a simple bubble column (BC) bioreactor, which promotes efficient mixing and cross-membrane gas transfer, with static fermentation, a traditional method lacking agitation and aeration. The study involved the parallel cultivation of <i>K. oxytoca M5A1</i> in both systems, with key parameters such as microbial growth, glucose utilization, protein concentration, and metabolite profiles monitored over a 48 h period. The results indicate that the BC bioreactor consistently outperformed static fermentation regarding the growth rate, protein yield, and glucose utilization efficiency. The BC exhibited a significant increase in protein production, reaching 299.90 µg/mL at 48 h, compared to 219.44 µg/mL in static fermentation. The organic acid profile reveals both synthesis and utilization regimes of varying patterns. These findings highlight the advantages of the BC bioreactor for MP production, particularly its ability to maintain aerobic conditions that support higher growth and yield.</p>","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"13 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2024-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11503442/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142509494","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}