气泡柱生物反应器中氧合克雷伯氏菌 M5A1 的微生物蛋白质和代谢物谱。

IF 2.7 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
BioTech Pub Date : 2024-10-19 DOI:10.3390/biotech13040043
Tawakalt Ayodele, Musiliu Liadi, Abodunrin Tirmidhi Tijani, Kudirat Alarape, Christiana Bitrus, Clairmont L Clementson, Ademola Hammed
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引用次数: 0

摘要

微生物蛋白质(MPs)的生产已成为生物技术领域的一个重要焦点,其驱动力来自于对可持续和可扩展的传统蛋白质来源替代品的需求。本研究调查了利用固氮细菌克雷伯氏菌 M5A1 生产 MPs 的两种实验设置的功效。K. oxytoca M5A1 因其兼性厌氧生长和固定大气氮的能力而闻名,它为环境友好型蛋白质生产提供了一个前景广阔的途径。这项研究比较了促进高效混合和跨膜气体传输的简单气泡柱(BC)生物反应器与缺乏搅拌和曝气的传统方法--静态发酵的性能。研究在这两个系统中平行培养了 K. oxytoca M5A1,并在 48 小时内对微生物生长、葡萄糖利用、蛋白质浓度和代谢物概况等关键参数进行了监测。结果表明,BC 生物反应器在生长速度、蛋白质产量和葡萄糖利用效率方面始终优于静态发酵。与静态发酵的 219.44 微克/毫升相比,BC 生物反应器的蛋白质产量在 48 小时内大幅增加,达到 299.90 微克/毫升。有机酸谱显示了不同模式的合成和利用机制。这些发现凸显了碱性催化分解生物反应器生产 MP 的优势,特别是其维持有氧条件的能力,从而支持更高的生长和产量。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbial Protein and Metabolite Profiles of Klebsiella oxytoca M5A1 in a Bubble Column Bioreactor.

The production of microbial proteins (MPs) has emerged as a critical focus in biotechnology, driven by the need for sustainable and scalable alternatives to traditional protein sources. This study investigates the efficacy of two experimental setups in producing MPs using the nitrogen-fixing bacterium Klebsiella oxytoca M5A1. K. oxytoca M5A1, known for its facultative anaerobic growth and capability to fix atmospheric nitrogen, offers a promising avenue for environmentally friendly protein production. This research compares the performance of a simple bubble column (BC) bioreactor, which promotes efficient mixing and cross-membrane gas transfer, with static fermentation, a traditional method lacking agitation and aeration. The study involved the parallel cultivation of K. oxytoca M5A1 in both systems, with key parameters such as microbial growth, glucose utilization, protein concentration, and metabolite profiles monitored over a 48 h period. The results indicate that the BC bioreactor consistently outperformed static fermentation regarding the growth rate, protein yield, and glucose utilization efficiency. The BC exhibited a significant increase in protein production, reaching 299.90 µg/mL at 48 h, compared to 219.44 µg/mL in static fermentation. The organic acid profile reveals both synthesis and utilization regimes of varying patterns. These findings highlight the advantages of the BC bioreactor for MP production, particularly its ability to maintain aerobic conditions that support higher growth and yield.

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来源期刊
BioTech
BioTech Immunology and Microbiology-Applied Microbiology and Biotechnology
CiteScore
3.70
自引率
0.00%
发文量
51
审稿时长
11 weeks
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