IF 3.6

BioTech Pub Date : 2026-05-23 DOI: 10.3390/biotech15020036

Inês S Almeida, Eva M Salgado, António M A Ferreira, José C M Pires

{"title":"Evaluation of Oscillatory Flow Conditions for Microalgal CO2 Capture and Biomass Sedimentation Kinetics: Experimental and Mathematical Approach.","authors":"Inês S Almeida, Eva M Salgado, António M A Ferreira, José C M Pires","doi":"10.3390/biotech15020036","DOIUrl":"10.3390/biotech15020036","url":null,"abstract":"This study evaluates the oscillatory frequency and amplitude in an oscillatory flow reactor with smooth periodic constrictions (OFR-SPC) for the cultivation and harvesting of Chlorella vulgaris fed with an air stream with 5% (v/v) CO2. Their effect on biomass productivity, CO2 capture, nutrient removal, and sedimentation kinetics was assessed. Cultures were tested at frequencies of 0.5-2.5 Hz and amplitudes of 6-18 mm. At 2.5 Hz|6 mm, the system achieved the maximum biomass concentration (592 mgDW L-1), productivity (5.36 mgDW L-1 h-1), and CO2 fixation (8.34 mg L-1 h-1) as well as complete nitrogen removal and near-complete phosphorus removal (100% and 91%, respectively). Complete sedimentation occurred at 0.5 Hz|6 mm, with kinetics described by the Gompertz model (k = 4.60 h-1), confirming the feasibility of low-cost biomass recovery. Additionally, zeta potential positively influenced sedimentation but negatively affected productivity. Statistical analyses confirmed oscillation frequency and amplitude as key factors, establishing the OFR-SPC as a promising technology for microalgae-based efficient CO2 capture, nutrient removal, and low-cost biomass harvesting.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13297455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148319230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spectroscopy-Based Cell Culture Predictive Monitoring. 基于光谱的细胞培养预测监测。

IF 3.6

BioTech Pub Date : 2026-05-20 DOI: 10.3390/biotech15020035

Ahmed Kanfoud, Pascal Gerkens, Marie Bastin, Laurent Rondia, Florian Ceulemans, Karim Donnay, Bertrand Debuisseret, Thomas Cornet, Gael de Lannoy, Thibault Helleputte

引用次数: 0

Algae-Derived Bioactive Compounds as Platforms for Translational Biotechnology and Health Applications. 藻类衍生的生物活性化合物作为转化生物技术和健康应用的平台。

IF 3.6

BioTech Pub Date : 2026-05-15 DOI: 10.3390/biotech15020034

Hannah Morris, Zoe Coombes, Zeinab El Dor, Valerie J Rodrigues, Alla Silkina, Pietro Marchese, Mary Murphy, Jessica M M Adams, Frank Barry, Claudio Fuentes-Grünewald, Walid Rachidi, Deyarina Gonzalez

{"title":"Algae-Derived Bioactive Compounds as Platforms for Translational Biotechnology and Health Applications.","authors":"Hannah Morris, Zoe Coombes, Zeinab El Dor, Valerie J Rodrigues, Alla Silkina, Pietro Marchese, Mary Murphy, Jessica M M Adams, Frank Barry, Claudio Fuentes-Grünewald, Walid Rachidi, Deyarina Gonzalez","doi":"10.3390/biotech15020034","DOIUrl":"10.3390/biotech15020034","url":null,"abstract":"Marine macroalgae, microalgae, and associated microorganisms are increasingly recognised as valuable sources of bioactive compounds with applications across biotechnology and health. The environmental and ecological conditions they inhabit shape their metabolite diversity, leading to the production of high-value compounds such as sulphated polysaccharides, lipids, pigments, phenolics, and peptides. These compounds exhibit conserved biological activities that underpin potent antioxidant, anti-inflammatory, cytotoxic, and pro-regenerative effects with strong potential for translation. Although external factors drive rich metabolite diversity, continual variation can also lead to translational constraints including heavy-metal accumulation, inconsistency in extract composition, and regulatory complexity. This review examines the environmental drivers of metabolite diversity and the functional potential of bioactives derived from marine algae. We focus on their translational application within four areas of growing interest: nutraceuticals, cosmetics, regenerative medicine, and oncology, where emerging evidence suggests their promise as next-generation bioactive ingredients and therapeutic leads. In addition, insights from Irish and Welsh Small and Medium Enterprises (SMEs) are collated to identify key bottlenecks in commercialisation and the requirements for effective marine biodiscovery pipelines. We consider the importance of controlled cultivation, standardised analytics, preclinical testing platforms, and collaborative innovation ecosystems and highlight the need for coordinated scientific, technical, and regulatory advances to unlock the full translational potential of marine-derived compounds.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13214499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148036027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Earthworms Drastically and Differentially Modify the Bacteriomes and Mycobiomes of Sewage Sludge. 蚯蚓对污水污泥的细菌群和真菌群的显著和差异修饰。

IF 3.6

BioTech Pub Date : 2026-05-10 DOI: 10.3390/biotech15020033

Marcos Pérez-Losada, Manuel Aira, Jorge Domínguez

{"title":"Earthworms Drastically and Differentially Modify the Bacteriomes and Mycobiomes of Sewage Sludge.","authors":"Marcos Pérez-Losada, Manuel Aira, Jorge Domínguez","doi":"10.3390/biotech15020033","DOIUrl":"10.3390/biotech15020033","url":null,"abstract":"Sewage sludge management poses major environmental challenges due to increasing production and concerns about contaminants and microbial risks. Vermicomposting offers a sustainable biological treatment, yet the extent to which different earthworm species shape microbial outcomes remains poorly understood. Here, we examined how gut transit by three epigeic (Eisenia andrei, E. fetida, and Dendrobaena hortensis) and two anecic (Lumbricus friendi and L. terrestris) earthworm species alters bacterial and fungal communities in fresh sewage sludge. Using 16S rRNA and ITS amplicon sequencing combined with multivariate, differential-abundance, and functional prediction analyses, we compared sludge and earthworm cast bacteriomes and mycobiomes. Earthworm gut transit caused pronounced species-specific restructuring of bacterial and fungal community composition, diversity, and functional profiles, with clear separation between sludge and cast communities. Functional analyses indicated coordinated shifts in bacterial metabolic potential and fungal trophic modes consistent with enhanced biosynthetic and decomposer functions. Pathogen profiles were reshaped in a host-dependent manner, with low overall abundances and selective changes rather than uniform suppression. These findings demonstrate that vermicomposting outcomes depend strongly on earthworm species and microbial kingdom, highlighting the importance of earthworm lifestyle diversity when evaluating the ecological safety and agronomic potential of sludge-derived amendments.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13214669/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148036062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus vallismortis Bioextracts Combined with Cold Plasma Air for Post-Harvest Fungi Biocontrol in Tomato (Lycopersicum solanum). vallismortis芽孢杆菌生物提取物与冷等离子体空气联合防治番茄采后真菌

IF 3.6

BioTech Pub Date : 2026-05-09 DOI: 10.3390/biotech15020032

Marisol Cruz Requena, Miguel A Medina-Morales, Paola Cano Reséndez, Leonardo Sepúlveda Torre, Thelma K Morales Martínez, Karina Reyes Acosta, Catalina Hernández Torres, Miriam Desiree Dávila Medina

{"title":"Bacillus vallismortis Bioextracts Combined with Cold Plasma Air for Post-Harvest Fungi Biocontrol in Tomato (Lycopersicum solanum).","authors":"Marisol Cruz Requena, Miguel A Medina-Morales, Paola Cano Reséndez, Leonardo Sepúlveda Torre, Thelma K Morales Martínez, Karina Reyes Acosta, Catalina Hernández Torres, Miriam Desiree Dávila Medina","doi":"10.3390/biotech15020032","DOIUrl":"10.3390/biotech15020032","url":null,"abstract":"Post-harvest diseases caused by phytopathogenic microorganisms generate significant economic losses, particularly in tomato crops affected by Alternaria solani. This study evaluated the effectiveness of cold plasma combined with a bioextract of Bacillus vallismortis as a biological strategy to extend tomato shelf life. In vitro antagonism assays were performed by confronting B. vallismortis against A. solani. Additionally, shelf-life tests were conducted on tomatoes treated with Bacillus cells and Bacillus cell-free bioextract (BCFB), followed by inoculation with A. solani spores, assessing incidence, severity, weight loss, and microbiological parameters over time. Subsequently, tomatoes were treated with cold plasma in combination with BCFB and reevaluated. Results showed significant antagonistic activity, with B. vallismortis and BCFB inhibiting A. solani by 75% and 50%, respectively. In untreated tomatoes, BCFB reduced disease incidence to 66.66% and severity to scale 2, compared to 100% incidence and scale 5 severity in controls. The combined treatment with cold plasma and BCFB showed the highest effectiveness, completely inhibiting A. solani (0% incidence, scale 0 severity), with tomatoes remaining healthy after 25 days. These findings demonstrate that cold plasma combined with B. vallismortis represents an effective and sustainable alternative for controlling post-harvest phytopathogens and extending tomato shelf life.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13214476/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148036072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Bio-Based Additives for Sustainable Polymers: A Systematic Review of Processing and Performance Enhancers. 可持续聚合物的功能性生物基添加剂：加工和性能增强剂的系统综述。

IF 3.6

BioTech Pub Date : 2026-04-22 DOI: 10.3390/biotech15020031

Odilon Souza Leite-Barbosa, Debora Cristina da Silva Santos, Cláudia Carnaval de Oliveira Pinto, Fernanda Cristina Fernandes Braga, Marcia Gomes de Oliveira, Marcelo Ferreira Leão de Oliveira, Valdir Florêncio da Veiga-Junior

{"title":"Functional Bio-Based Additives for Sustainable Polymers: A Systematic Review of Processing and Performance Enhancers.","authors":"Odilon Souza Leite-Barbosa, Debora Cristina da Silva Santos, Cláudia Carnaval de Oliveira Pinto, Fernanda Cristina Fernandes Braga, Marcia Gomes de Oliveira, Marcelo Ferreira Leão de Oliveira, Valdir Florêncio da Veiga-Junior","doi":"10.3390/biotech15020031","DOIUrl":"10.3390/biotech15020031","url":null,"abstract":"Background: The transition from fossil-derived polymer additives to renewable alternatives is essential to mitigate environmental persistence and ensure chemical safety within the plastics industry. This review provides a comprehensive overview of recent developments in bio-based functional additives and their integration into circular economy frameworks. Methods: Following PRISMA guidelines, a systematic literature search was conducted using the Scopus database for studies published between 2023 and 2026. Search terms targeted bio-based plasticizers, flame retardants, antioxidants, and compatibilizers. Studies were screened against predefined inclusion criteria, specifically focusing on experimental validation in polymer matrices, while data mining was employed to map emerging research fronts. Results: From an initial 996 records, 54 studies were selected after removing duplicates and ineligible articles. The findings highlight a paradigm shift from passive physical fillers toward active, multifunctional macromolecular agents. Recent literature demonstrates that targeted molecular interventions, such as phosphorylated lignin and biomimetic structures, can resolve trade-offs between ductility and thermal stability at low loadings (<5 wt%). Synthesis routes, performance outcomes, and end-of-life trajectories for each additive class are summarized. Conclusions: Bio-based additives have evolved from simple substitutes into strategic tools for the molecular programming of sustainable polymers. Although challenges regarding scalability and high-temperature processing persist, their integration into circular economy strategies establishes a clear roadmap for next-generation bioplastics.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108088/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Flow and Hybridization Potential Between GM/NGT Crops and Conventional Varieties or Wild Relatives: A Scoping Literature Review with Emphasis on Oilseed Rape (Brassica napus L.) and Potato (Solanum tuberosum L.). 转基因/NGT作物与常规品种或野生近缘种的基因流动和杂交潜力——以油菜和马铃薯为重点的文献综述

IF 3.6

BioTech Pub Date : 2026-04-08 DOI: 10.3390/biotech15020030

Lelde Grantina-Ievina, Nils Rostoks

{"title":"Gene Flow and Hybridization Potential Between GM/NGT Crops and Conventional Varieties or Wild Relatives: A Scoping Literature Review with Emphasis on Oilseed Rape (Brassica napus L.) and Potato (Solanum tuberosum L.).","authors":"Lelde Grantina-Ievina, Nils Rostoks","doi":"10.3390/biotech15020030","DOIUrl":"10.3390/biotech15020030","url":null,"abstract":"Genetically modified (GM) plants have been commercially grown for 30 years, and their acceptance depends on a thorough risk assessment. Environmental Risk Assessment (ERA) evaluates potential impacts of releasing GM plants into the environment, whether through cultivation or import for food, feed, and processing. A key component is assessing potential gene flow to crop wild relatives or non-GM crops. For gene flow to significantly affect the environment, transferred genes must provide a selective advantage. Since most GM plants are engineered for herbicide tolerance, insect resistance, or stacked traits, evaluating such advantages is relatively straightforward. New genomic techniques (NGTs) can generate plants with a wider range of traits, including tolerance to biotic and abiotic stress. Although still considered GM in the EU, their genomic changes can complicate detection, identification, and ERA, especially when such traits may offer advantages under stress conditions. This scoping review focuses on gene flow in two crops: oilseed rape (canola) (Brassica napus L.) and potato (Solanum tuberosum L.). In canola, transgene movement can increase weediness, fitness, herbicide resistance, or genetic diversity in feral or related populations. Gene flow in potato is less studied, with concerns centered on contamination risks in the Andean diversity center. Limited data exist for NGT plants, though many are expected to resemble conventionally bred varieties, suggesting comparable environmental impacts.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DFT Calculations on Electronic, Thermochemical and Vibrational Properties of Se₆ Selenium Clusters as 5-Fluorouracil Drug Delivery System. 5-氟尿嘧啶给药体系Se6硒团簇的电子、热化学和振动性质的DFT计算

IF 3.6

BioTech Pub Date : 2026-03-31 DOI: 10.3390/biotech15020029

Levi Isai Solano-González, Raúl Mendoza-Báez, Ricardo Agustín-Serrano, José Isrrael Rodríguez-Mora, Marco A Morales

{"title":"DFT Calculations on Electronic, Thermochemical and Vibrational Properties of Se6 Selenium Clusters as 5-Fluorouracil Drug Delivery System.","authors":"Levi Isai Solano-González, Raúl Mendoza-Báez, Ricardo Agustín-Serrano, José Isrrael Rodríguez-Mora, Marco A Morales","doi":"10.3390/biotech15020029","DOIUrl":"10.3390/biotech15020029","url":null,"abstract":"In this work, the electronic, thermochemical, and vibrational characterization of the drug delivery system formed by clusters of selenium (Se6 allotrope) and 5-fluorouracil (5-FU) are studied, based on density functional theory (DFT) calculations. Computational calculations were performed using the B3LYP functional and the 6-31G(d,p) base set, considering an aqueous medium through the CPCM solvation model. We propose evaluating two different interaction modes based on experimental observations: Se-H(N) (through the amino groups of 5-FU) and Se-O(C) (through the carbonyl oxygen of 5-FU). All complexes proved to be energetically stable, exhibiting chemisorption as their adsorption process. Analysis of adsorption energy and thermodynamic parameters indicates that both interaction pathways are equally viable, which agrees with previous experimental findings. The theoretical FT-IR spectra of these complexes also coincide with the experimental results. Furthermore, global molecular descriptors show that the stability of the selenium carrier is not affected by post-functionalization, which is desirable for more controlled drug delivery systems.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108189/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hypoxia and DNA-Repair Radiosensitivity Signatures Are Associated with Radiotherapy-Modified Survival in TCGA Breast Cancer, with External Prognostic Validation of the Hypoxia Score in METABRIC. 缺氧和dna修复放射敏感性特征与TCGA乳腺癌放疗改善生存率相关，并在METABRIC中对缺氧评分进行外部预后验证。

IF 3.6

BioTech Pub Date : 2026-03-31 DOI: 10.3390/biotech15020028

Jimmy Carter Osei, Mei-Han Chen, Tim A D Smith

{"title":"Hypoxia and DNA-Repair Radiosensitivity Signatures Are Associated with Radiotherapy-Modified Survival in TCGA Breast Cancer, with External Prognostic Validation of the Hypoxia Score in METABRIC.","authors":"Jimmy Carter Osei, Mei-Han Chen, Tim A D Smith","doi":"10.3390/biotech15020028","DOIUrl":"10.3390/biotech15020028","url":null,"abstract":"Radiotherapy (RT) is one of the main treatments for breast cancer, but response varies between patients. Tumour hypoxia and intrinsic radiosensitivity are major determinants of response to RT. Using TCGA-BRCA, a 563-gene hypoxia meta-signature was built by combining curated hypoxia gene sets from MSigDB with published hypoxia metagenes (Buffa, Winter, Elvidge, Fardin, and related sets). After Cox screening and penalised regression, a simple three-gene hypoxia score (CP, GPC3, STC1) was derived. In parallel, based on DSB-repair factors highlighted by Mladenov et al. as key regulators of intrinsic radiosensitivity, a four-gene radiosensitivity (RS) signature (ATR, RPA2, BLM, MRE11A) was trained using only RT-treated patients. In TCGA, both signatures were prognostic and showed significant interaction with RT status in Cox models. The hypoxia score was strongly associated with worse outcomes in RT-untreated patients, but this effect was much weaker in RT-treated patients (Hypoxia × RT HR = 0.009, p = 0.044). The RS score showed a similarly strong interaction with RT (RS × RT HR = 0.011, p = 0.003). When we combined both signatures into one interaction model, it gave the best performance (C-index = 0.785), and both interaction terms stayed independently significant. The hypoxia score was then validated externally in METABRIC (N = 1979; 1143 events), where it remained associated with overall survival, although more weakly than in TCGA (HR = 1.34, 95% CI: 1.10-1.63; p = 0.0042). Overall, these results suggest that hypoxia and DSB-repair capacity capture two complementary sides of radiosensitivity and RT-modified survival patterns, and they support further prospective testing and validation in independent datasets with strong RT annotation.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioremediation of Lubricant Oil by Environmentally Adapted Pseudomonas aeruginosa, Pseudomonas putida, and Proteus vulgaris in Houston, Texas. 环境适应性铜绿假单胞菌、恶臭假单胞菌和寻常变形杆菌对润滑油的生物修复。

IF 3.6

BioTech Pub Date : 2026-03-26 DOI: 10.3390/biotech15020027

Sadith Mosquera, Jason A Rosenzweig

{"title":"Bioremediation of Lubricant Oil by Environmentally Adapted Pseudomonas aeruginosa, Pseudomonas putida, and Proteus vulgaris in Houston, Texas.","authors":"Sadith Mosquera, Jason A Rosenzweig","doi":"10.3390/biotech15020027","DOIUrl":"10.3390/biotech15020027","url":null,"abstract":"Lubricating oil (LO) is manufactured in various formulations for different applications. The inappropriate disposal of petroleum hydrocarbons can increase soil contamination, promoting deleterious environmental and human health impacts. More specifically, following prolonged exposure, LO contaminants are known to have carcinogenic and neurotoxic effects in humans. Bioremediation provides an effective and attractive strategy to expedite the clean-up processes of LO contaminants. We isolated and identified environmentally adapted strains of Pseudomonas aeruginosa, Pseudomonas putida, and Proteus vulgaris from Houston watershed bayou soils. Interestingly, all three exhibited increased resistance, vis-a-vis surrogate strains, to various antibiotic challenges (of chloramphenicol, tetracycline, kanamycin, penicillin, streptomycin, etc.) and increased biofilm formation ranging from 1.6 to 6.7-fold. In fact, all three environmental strains were significantly better at producing enhanced biofilm formation in the presence of spent LO rather than clean LO as well as outproducing biofilm made by the surrogate strains. Finally, the environmental isolates P. aeruginosa, P. putida, and P. vulgaris demonstrated an enhanced ability to sequester clean (2-, 2.5- and 1.14-fold) and spent (1.4-, 1.5, and 1.2-fold) LO when compared to their commercially acquired surrogate reference strains. Our three environmentally isolated organisms from Houston watershed soils appeared to be environmentally adapted to tolerate LO exposures. In the presence of LOs, all three environmentally isolated strains exhibited enhanced growth, enhanced biofilm production, and improved bioaccumulation of LOs relative to commercial reference strains. Taken together, environmentally adapted organisms can promote the bioremediation of contaminants threatening our environment and, potentially, human health.","PeriodicalId":34490,"journal":{"name":"BioTech","volume":"15 2","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13108208/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147783243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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