2009 International Symposium on Micro-NanoMechatronics and Human Science最新文献_第6页

Detection and collection system of target single cell based on respiration and metabolic activity 基于呼吸和代谢活性的目标单细胞检测和收集系统

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351919

Masayasu Suzuki, Hiroyuki Tanaka, Y. Iribe

引用次数: 3

Multiple object detection for intelligent robot vision by using growing neural gas 基于生长神经气体的智能机器人视觉多目标检测

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352076

H. Sasaki, N. Kubota, K. Sekiyama, T. Fukuda

引用次数: 10

Study on optimization of exposure energy distribution for fabrication of arbitrary 3-D microstructure by shaped beam using synchrotron radiation 同步辐射成形光束制备任意三维微结构的辐照能量分布优化研究

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351837

M. Horade, S. Sugiyama

{"title":"Study on optimization of exposure energy distribution for fabrication of arbitrary 3-D microstructure by shaped beam using synchrotron radiation","authors":"M. Horade, S. Sugiyama","doi":"10.1109/MHS.2009.5351837","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351837","url":null,"abstract":"Research on establishment of a fabrication method of three-dimensional microstructure which used SR (Synchrotron Radiation) light is reported. As the advantage of this method, since there is no necessity of fabrication masks and it is suitable for rapid prototyping, reduction of fabrication time and cost is mentioned. In this time, we carry out basic research about realization of this fabrication method, and it succeeded in fabrication of the free-form surface by exposure between pixels overlap. Moreover, in order to fabricate the three-dimensional structure of arbitrary shape, the algorithm which can determine exposure energy amount in consideration of overlap was written to target form. Using this algorithm, fabrication of various arbitrary three-dimensional structures was possible by only single aperture without manufacture a mask for every form. It is examining using this technique for fabrication of μ-TAS or a reactor. The channel which has the inclination and free-form surface are fabricated by this method, improvement in functionality is promising.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128880892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of micro particles separation device with piezo-ceramic vibrator 压电陶瓷振动器微颗粒分离装置的研制

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352095

K. Ooe, T. Fukuda

{"title":"Development of micro particles separation device with piezo-ceramic vibrator","authors":"K. Ooe, T. Fukuda","doi":"10.1109/MHS.2009.5352095","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352095","url":null,"abstract":"During hemanalysis, it is necessary to separate blood cells from whole blood. Many blood separation methods, for example, centrifugation and filtering, are in practical use. However, the use of these methods involves problems from the perspectives of processing speed and processing volume. We develop new types of blood separation devices that use piezo-ceramic vibrators. The first device uses a capillary. One end of the capillary is fixed to the device frame, and the other is fixed to a piezo-ceramic vibrator. The vibrator transmits bending waves to the capillary. This device can process only a small amount of solution; therefore, it is not suitable for hemanalysis. In order to solve this problem, we developed a second device; this device has a pair of thin glass plates with a small gap as a substitute for the capillary used in the first device. These devices are based on the fact that particles heavier than water move toward transverse velocity antinodes while those lighter than water move toward velocity nodes. In this report, we demonstrate the high-speed separation of silica microbeads and 50-vol% glycerol water by using these devices. The first device can separate the abovementioned solution within 3 min while the second can separate it within 1 min. Both devices are driven by a rectangular wave of 15 to 20 Vpp. Furthermore, it has been confirmed that red blood cells are separated from diluted whole blood using the first device within approximately 1 min. These devices have transparency, so they can compose as the analysis system with the chemical analyzer easily.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129845717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

On-chip magnetically driven micro-robot for enucleation of oocyte 用于卵母细胞去核的片上磁驱动微型机器人

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351824

N. Inomata, T. Mizunuma, Y. Yamanishi, S. Kudo, F. Arai

引用次数: 7

Micro orifice based cell pairing and fusion on microfluidic chip 微流控芯片上基于微孔的细胞配对与融合

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351836

M. Gel, Y. Mori, Y. Kimura, O. Kurosawa, B. Techaumnat, H. Oana, M. Washizu

{"title":"Micro orifice based cell pairing and fusion on microfluidic chip","authors":"M. Gel, Y. Mori, Y. Kimura, O. Kurosawa, B. Techaumnat, H. Oana, M. Washizu","doi":"10.1109/MHS.2009.5351836","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351836","url":null,"abstract":"Micro orifice assisted cell fusion assures high-yield fusion without compromising the cell viability. This paper examines feasibility of a cell pairing method compatible with micro orifice based cell fusion to create large number of viable fusants for studying post-fusion cell behavior. We fabricated a microfluidic chip which contained a chamber and a partition. The partition divided the chamber into two compartments and it had a number of embedded micro orifices. The voltage applied to the electrodes located at each compartment generated electric field distribution concentrating in each micro orifice. Cells introduced into each compartment moved towards micro orifice by manipulation of hydrostatic pressure. Dielectrophoretic force trapped the cells in micro orifice and established cell to cell contact through orifice. By applying a pulse, cell fusion was initiated to form a neck between cells. The neck passing through orifice resulted in immobilization of the fused cell pair. Unfused cells washed away. Then, the chip was loaded to a microscope stage incubator for time lapse imaging of the immobilized fusants. The viable fusants were successfully generated by fusion of mouse fibroblast cells. Time lapse observation of the five fusants showed that fused cell pairs were released from micro orifice and became one body. Fusants which reached to cell division phase divided into three daughter cells. We conclude that the presented method of cell pairing and fusion is suitable for high-yield generation of viable fusants and studying of post-fusion phenomena.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122577923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Features of ultra thin poly(N-isopropylacrylamide) grafted onto glass cover slips 超薄聚(n -异丙基丙烯酰胺)接枝到玻璃盖板卡瓦上的特性

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351981

Y. Akiyama, K. Fukumori, M. Yamato, K. Sakai, T. Okano

{"title":"Features of ultra thin poly(N-isopropylacrylamide) grafted onto glass cover slips","authors":"Y. Akiyama, K. Fukumori, M. Yamato, K. Sakai, T. Okano","doi":"10.1109/MHS.2009.5351981","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351981","url":null,"abstract":"Ultra thin temperature responsive polymer, poly(N-isopropylacrylamide) (PIPAAm) grafted layer exhibits cell attachment and detachment properties in response to temperature change. Such properties are dependent on thickness and amount of the grafted PIPAAm, and are observed for extremely ultra thin thickness (for example, 20 nm thickness for tissue culture poly styrene and 5 nm for glass cover slips). Although this phenomenon was not demonstrated clearly, we have proposed a mechanism. Namely, thinner PIPAAm layer are subjected to dehydration of the PIPAAm chains in the vicinity of basal surfaces. The dehydration progressively promotes dehydration of the grafted PIPAAm chains toward to the outermost regions. In this presentation, we tried to demonstrate the proposed mechanism by investigating the dynamic alternation of thickness of PIPAAm layer in response to temperature. The alternation was measured undere aqueous conditions above and below LCST, using AFM. As a result, thickness of thinner PIPAAm layer was not altered between under atmospheric and aqueous conditions, while thicker layer was done. This difference strongly support the proposed mechanism.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122809276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultra miniature µ-accelerometer for wearable physical activity monitoring systems 用于可穿戴式身体活动监测系统的超微型微加速度计

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351973

R. Amarasinghe, D. Dao, S. Sugiyama

引用次数: 1

Analysis of cellular membrane changing induced by influenza virus infection 流感病毒感染引起细胞膜改变的分析

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351929

Takuro Takahata, S. Kume, H. Miyoshi, T. Sugiura, A. Honda

引用次数: 0

Spontaneous formation of giant unilamellar vesicles from microdroplets of a polyion complex by focused infrared laser irradiation 聚焦红外激光照射多离子复合物微滴自发形成巨大单层囊泡

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352051

H. Oana, A. Kishimura, Y. Yamasaki, M. Washizu, K. Kataoka

引用次数: 0