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Stem Cells: DNA Microarray Approach 干细胞:DNA微阵列方法
International Journal of Ecophysiology Pub Date : 2023-01-24 DOI: 10.32734/ijoep.v4i1.11151
S. Ilyas
{"title":"Stem Cells: DNA Microarray Approach","authors":"S. Ilyas","doi":"10.32734/ijoep.v4i1.11151","DOIUrl":"https://doi.org/10.32734/ijoep.v4i1.11151","url":null,"abstract":"In recent years, stem cell therapy has become a very promising and advanced scientific research topic. The development of treatment methods has evoked great expectations, not only researchers but also society due to its potency in cell-based therapy. Stem cells are unspecialized cells of the human body. They are able to differentiate into any cell of an organism and have the ability of self-renewal. Broadly speaking, the application of stem cells can be divided into two, namely: autotransplantation (donor and recipient are same person) and allotransplantation (donors and recipients are people who different). However, other applications namely xenotransplantation (donor and the recipient is the species that different) now it also becomes center of attention. Since it is challenging to isolate stem cells, molecular methods specifically the use of a DNA microarray, are one way to find stem cells. Microarrays have been applied in stem cell research to identify major features or expression signatures that define stem cells and characterize their differentiation programs toward specific lineages. This paper is a review focused on a discussion of the of the microarray technology and types of stem cell transplant.","PeriodicalId":344109,"journal":{"name":"International Journal of Ecophysiology","volume":"93 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123130798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ethnobotany of the Vile Shard Plant (strobilanthes crispa (L) blume) As a Cure for Diabetes in the People of the Nias Islands 作为治疗尼亚斯群岛人民糖尿病的一种治疗方法的恶碎片植物(strobilanthes crispa (L) blume)的民族植物学
International Journal of Ecophysiology Pub Date : 2023-01-24 DOI: 10.32734/ijoep.v4i1.11174
E. Siregar
{"title":"Ethnobotany of the Vile Shard Plant (strobilanthes crispa (L) blume) As a Cure for Diabetes in the People of the Nias Islands","authors":"E. Siregar","doi":"10.32734/ijoep.v4i1.11174","DOIUrl":"https://doi.org/10.32734/ijoep.v4i1.11174","url":null,"abstract":"The use of medicinal plants by the Indonesian people has been carried out for a long time from generation to generation to cure certain diseases, one of which is the people of the Nias Islands who use the vile shard leaves ( Strobilanthes crispa (L. ) (Blume) as a drug to reduce urinary complaints, kidney stones, gallstones and diabetes. Keji shard leaves are known to have high antioxidant compounds, namely flavonoids which can be used as medicinal compounds to treat diabetes, hypertension and inhibit the growth of pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Streptococcus pyogenes","PeriodicalId":344109,"journal":{"name":"International Journal of Ecophysiology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133649269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Antimicrobe of Kaempferia galanga L. Rhizome against Microsporum canis and Staphylococcus epidermidis – In-vitro Study 山奈良姜根茎对犬小孢子菌和表皮葡萄球菌的体外抑菌研究
International Journal of Ecophysiology Pub Date : 2023-01-24 DOI: 10.32734/ijoep.v4i1.11167
Kiki Nurtjahtja
{"title":"The Antimicrobe of Kaempferia galanga L. Rhizome against Microsporum canis and Staphylococcus epidermidis – In-vitro Study","authors":"Kiki Nurtjahtja","doi":"10.32734/ijoep.v4i1.11167","DOIUrl":"https://doi.org/10.32734/ijoep.v4i1.11167","url":null,"abstract":"This study aims to determine the ability of ethanolic extract of Kaempferia galanga L. rhizome in inhibiting the growth of Microsporum canis and Staphylococcus epidermidis. Rhizome concentrations used were 40, 50, 60, and 70%. Each treatment was replicate 4 times. Results showed that extract of K. galanga rhizome up to 70% have not significantly differences against M. canis and S. epidermidis. Rhizome extract of 70% inhibit the growth of M. canis with the highest inhibition 5.88 mm (in compared to ketoconazole 10.35 mm). Whereas the extract of 70% inhibit S. epidermidis with the highest inhibition 3.16 mm (in compared to chloramphenicol 25.49 mm).","PeriodicalId":344109,"journal":{"name":"International Journal of Ecophysiology","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122738624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Review Journals : Molecular From Anonaceae 综述期刊:菊科分子
International Journal of Ecophysiology Pub Date : 2023-01-24 DOI: 10.32734/ijoep.v4i1.11152
S. Ilyas
{"title":"Review Journals : Molecular From Anonaceae","authors":"S. Ilyas","doi":"10.32734/ijoep.v4i1.11152","DOIUrl":"https://doi.org/10.32734/ijoep.v4i1.11152","url":null,"abstract":"There are roughly 120 species of the Anona genus, which is found in tropical and subtropical areas. The diversity at the species level and similarity of the leaves, fruits, and stems that are produced as a result of plant cultivation and hybridization create new plants that are difficult to macroscopically identify. The diversity of the genus Anona can be accurately determined using molecular identification. For example, genetic analysis utilizing PCR and the RAPD and AFLP procedures is one way to gather molecular data. In this study, we inform the development of species-specific primers by differentiating the most agronomically interesting Annona species that, until recently, a new formal classification based on molecular phylogenetics has been developed in order to have a quick and precise method of distinguishing Annona between species agronomically.","PeriodicalId":344109,"journal":{"name":"International Journal of Ecophysiology","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127809376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Utilization Of Lime Peel Waste (Citrus aurantifolia (Christm.) Swingle) As An Antibacterial Against Salmonella typhi 柑桔果皮废弃物的利用作为对抗伤寒沙门氏菌的抗菌药物
International Journal of Ecophysiology Pub Date : 2023-01-24 DOI: 10.32734/ijoep.v4i1.11173
H. Simanjuntak
{"title":"Utilization Of Lime Peel Waste (Citrus aurantifolia (Christm.) Swingle) As An Antibacterial Against Salmonella typhi","authors":"H. Simanjuntak","doi":"10.32734/ijoep.v4i1.11173","DOIUrl":"https://doi.org/10.32734/ijoep.v4i1.11173","url":null,"abstract":"Typhoid fever is a digestive tract infection caused by Salmonella typhi. Treatment of typhoid fever can use natural ingredients such as lime peel waste. The purpose of this study was to determine the effect of the ethanol extract of lime peel waste on the growth of Salmonella typhi bacteria. The extraction method used the maceration technique, and the antibacterial activity test used the paper disc diffusion method. The results showed that the ethanol extract of lime peel waste at concentrations of 1 mg/ml, 3 mg/ml, 5 mg/ml and 7 mg/ml with inhibition zone diameters of 4.66 mm, 6.55 mm, 9.29 mm and 10.06 mm. The conclusion shows that there is an effect of lime peel ethanol extract in inhibiting the growth of Salmonella typhi bacteria in the weak to strong category.","PeriodicalId":344109,"journal":{"name":"International Journal of Ecophysiology","volume":"64 1-2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114131768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Immunohistochemistry examination to reveal the pathogenesis of Oral Squamous Cell Carcinoma 免疫组化检查揭示口腔鳞状细胞癌的发病机制
International Journal of Ecophysiology Pub Date : 2023-01-24 DOI: 10.32734/ijoep.v4i1.11150
S. Ilyas
{"title":"Immunohistochemistry examination to reveal the pathogenesis of Oral Squamous Cell Carcinoma","authors":"S. Ilyas","doi":"10.32734/ijoep.v4i1.11150","DOIUrl":"https://doi.org/10.32734/ijoep.v4i1.11150","url":null,"abstract":"Oral mucosal cancer is a type of cancer that develops from the lining of the oral cavity (mucosa). The main risk factors are smoking and drinking alcohol. The pathogenesis of oral mucosal cancer involves various interrelated etiologies such as smoking and alcohol consumption, human papilloma virus (HPV), and patients who have undergone hemopoietic stem cell transplants (stem cell transplants). Meanwhile, it does not rule out the possibility of internal factors such as genetics. There are several types of oral mucosal cancer, but oral squamous cell carcinoma is the most common type of oral cancer and represents more than 90% of all head and neck cancers. Immunohistochemical examination of the Oral squamous cell carcinoma smear biopsy material which included examination of antibodies in the form of cytokeratin, CDT1, Ki-67, Geminin, Mcm2, γ-H2A/ histone family member X and Aurora-A, BCL-2, VEGF, p53, p63, p,73, Prb, c-erbB2 or HER2/neu, upregulation of telomerase (human telomerase reverse transcriptase; hTERT), loss of heterozygosity (Chromosome loci 3p, 8p, 9p, 4q, 11q, 13q, 17p), High-risk Human papillomavirus 16/18 (HR-HPV16 and 18), p16, Overexpression of EGFR, c-jun, c-fos, surviving (BIRC5), MMP-9, MMP9, TGF-, COX-1, and-2, and amplification of Cyclins D and E. Thus, paving the way for appropriate therapy.","PeriodicalId":344109,"journal":{"name":"International Journal of Ecophysiology","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117141249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Macrofungi Diversity in Mount Pandan Ectourism Area, Aceh Tamiang 亚齐·Tamiang潘兰山生态旅游区大型真菌多样性研究
International Journal of Ecophysiology Pub Date : 2023-01-24 DOI: 10.32734/ijoep.v4i1.11175
Juli Trianda Sri Rejeki
{"title":"Macrofungi Diversity in Mount Pandan Ectourism Area, Aceh Tamiang","authors":"Juli Trianda Sri Rejeki","doi":"10.32734/ijoep.v4i1.11175","DOIUrl":"https://doi.org/10.32734/ijoep.v4i1.11175","url":null,"abstract":"Macrofungi are fungi that can be seen with the naked eye. Its characteristics can be seen based on its structure and body shape. Generally, fungi live in various types of habitats, namely wood, soil, litter, animal waste and so on. This research was conducted on 8-9 June 2022 in the Ecotourism area of Gunung Pandan, Aceh Tamiang Regency, Aceh Province. Determining the sampling point was carried out by the path method by observing macroscopic fungi along the hiking trail at a distance of 10 meters to the right and 10 meters. The most numerous macroscopic fungi, living solitary and these fungi were found in the Basidiomycota division, with 11 species and the least in the Thallophyta division, Eumycetes class, with 1 species.","PeriodicalId":344109,"journal":{"name":"International Journal of Ecophysiology","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115104593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Human Papillomavirus: Detection Method and Infection 人乳头瘤病毒:检测方法及感染
International Journal of Ecophysiology Pub Date : 2023-01-24 DOI: 10.32734/ijoep.v4i1.11153
S. Ilyas
{"title":"Human Papillomavirus: Detection Method and Infection","authors":"S. Ilyas","doi":"10.32734/ijoep.v4i1.11153","DOIUrl":"https://doi.org/10.32734/ijoep.v4i1.11153","url":null,"abstract":"The Human Papilloma Virus (HPV) is a small non-enveloped DNA virus, around 8000 bp in size and only humans become its host by infecting skin epithelial tissue, human oral mucosa and anogenital epithelium. Human Papillomavirus is often found in patients and is ranked as the second most malignant disease in women, belonging to the Alphapapillomavirus genus. HPV infection can be identified through the structure of the HPV virus itself and the particles contained therein which initiate the carcinogenic process of its host. The research methods used in this study are literature studies. The literature study method is a series of activities related to the method of collecting library data, reading, recording and managing research materials. The method often used to detect HPV is the polymerase chain reaction (PCR). The viral cell reproduction process is aided by activated proteins E1, E2, E4, E5, E6, and E7, which also delay apoptosis and alter the host cell cycle in various ways to cause cell alterations that result in cancer cells.","PeriodicalId":344109,"journal":{"name":"International Journal of Ecophysiology","volume":"213 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125984330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Retardan Variation of Paclobutrazol in VW (Vacin & Went) Media Towards the Artificial seeds Viability of the Dendrobium sp. 多效唑在VW (Vacin & go)培养基中对石斛人工种子活力的影响
International Journal of Ecophysiology Pub Date : 2023-01-24 DOI: 10.32734/ijoep.v4i1.11165
I. Nurwahyuni, Deny Supriharti, Immanuel Y. N. Sitorus
{"title":"Retardan Variation of Paclobutrazol in VW (Vacin & Went) Media Towards the Artificial seeds Viability of the Dendrobium sp.","authors":"I. Nurwahyuni, Deny Supriharti, Immanuel Y. N. Sitorus","doi":"10.32734/ijoep.v4i1.11165","DOIUrl":"https://doi.org/10.32734/ijoep.v4i1.11165","url":null,"abstract":"The research of the variation of paclobutrazol retardant in VW (Vacin & Went) media toward the viability of artificial seeds of Dendrobium sp. had been carried out at the Laboratory of Physiology and Plant Tissue Culture, Department of Biology, Faculty of Mathematics and Natural Sciences, University of North Sumatra, Medan. The purpose of the study was to obtain the best combination of paclobutrazol treatments and the storage time on the viability of artificial seeds of Dendrobium sp. This study used a factorial completely randomized design by two factors, which were six levels of paclobutrazol 0; 1; 2; 3; 4; 5 ppm, and 2 levels of storage duration times (0; 2; 4 weeks). The statistical analysis of the data  showed that the combination of paclobutrazol treatments and storage times significantly affected the growth time of plb Dendrobium sp. as well as the wet weight and the dry weight (P < 0.05). Paclobutrazol concentration of 4 ppm and storage period of 4 weeks (P4L2) gave the best effect in delaying the growth of synthetic seeds of Dendrobium sp. with an average growth of 21.6 days. The 2 ppm treatment of paclobutrazol and the 4 weeks storage time (P2L2) gave the highest wet weight and dry weight by an average of 0.198 grams and 0.007760 grams, respectively.","PeriodicalId":344109,"journal":{"name":"International Journal of Ecophysiology","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134550058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Community Composition Of Top Soil Collembola In The Land Application Area Of Liquid Waste Oil Palm Plantation Factory Simpang Kanan District Rokan Hilir Regency 新邦加南区罗干希利县废油棕榈园用地区表层土壤线虫群落组成
International Journal of Ecophysiology Pub Date : 2022-11-16 DOI: 10.32734/ijoep.v3i2.10093
A. H. John
{"title":"Community Composition Of Top Soil Collembola In The Land Application Area Of Liquid Waste Oil Palm Plantation Factory Simpang Kanan District Rokan Hilir Regency","authors":"A. H. John","doi":"10.32734/ijoep.v3i2.10093","DOIUrl":"https://doi.org/10.32734/ijoep.v3i2.10093","url":null,"abstract":"This research has been conducted to knowing and analyze the community composition of top soil Collembola in the land application area oil palm plantation at Simpang Kanan District, Rokan Hilir  Regency.  Sampling  Area  Assessed  Through “Purposive Random Sampling”  in which top soil Collembola which were collected by using Pitfall Trap. Location of sampling area through at three location. The first location at block 1 as control, the second location at block 2 which by using land application Flatbed system, and the third location at block 3 which by using land application longbed system. There were fourteen species of Collembola classified into three families and one order. The highest value of  species compotition at first location as control was Entomobrya sp.1 amount 27.907 %. The second location (Block 2) which by using Flatbed system the highest value of  species compotition was Entomobrya sp.3 amount 35.439%. The third  location  (Block  3)  which  by  using  Longbed  system,  the  highest  value  of    species compotition was Entomobrya sp.1  amount 27.076 %.","PeriodicalId":344109,"journal":{"name":"International Journal of Ecophysiology","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114530879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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