Retardan Variation of Paclobutrazol in VW (Vacin & Went) Media Towards the Artificial seeds Viability of the Dendrobium sp.

I. Nurwahyuni, Deny Supriharti, Immanuel Y. N. Sitorus
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Abstract

The research of the variation of paclobutrazol retardant in VW (Vacin & Went) media toward the viability of artificial seeds of Dendrobium sp. had been carried out at the Laboratory of Physiology and Plant Tissue Culture, Department of Biology, Faculty of Mathematics and Natural Sciences, University of North Sumatra, Medan. The purpose of the study was to obtain the best combination of paclobutrazol treatments and the storage time on the viability of artificial seeds of Dendrobium sp. This study used a factorial completely randomized design by two factors, which were six levels of paclobutrazol 0; 1; 2; 3; 4; 5 ppm, and 2 levels of storage duration times (0; 2; 4 weeks). The statistical analysis of the data  showed that the combination of paclobutrazol treatments and storage times significantly affected the growth time of plb Dendrobium sp. as well as the wet weight and the dry weight (P < 0.05). Paclobutrazol concentration of 4 ppm and storage period of 4 weeks (P4L2) gave the best effect in delaying the growth of synthetic seeds of Dendrobium sp. with an average growth of 21.6 days. The 2 ppm treatment of paclobutrazol and the 4 weeks storage time (P2L2) gave the highest wet weight and dry weight by an average of 0.198 grams and 0.007760 grams, respectively.
多效唑在VW (Vacin & go)培养基中对石斛人工种子活力的影响
在棉兰北苏门答腊大学数学与自然科学学院生物系生理与植物组织培养实验室进行了多氯丁唑(paclobutrazol)在VW (Vacin & go)培养基中对石斛人工种子活力的影响研究。本研究旨在获得多效唑处理和贮藏时间对石斛人工种子活力的最佳组合。本研究采用两因素全随机设计,即6个水平多效唑0;1;2;3;4;5 ppm和2级储存时间(0;2;4周)。数据统计分析表明,多效唑处理和贮藏次数组合显著影响了plb石斛的生长时间、湿重和干重(P < 0.05)。多效唑浓度为4 ppm,贮藏时间为4周(P4L2)对石斛合成种子的生长延迟效果最好,平均生长21.6 d。多效唑2 ppm处理和4周贮藏时间(P2L2)的湿重和干重最高,平均分别为0.198 g和0.007760 g。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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