The Journal of Cell Biology最新文献_第8页

Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole 植物自噬体在进入中央液泡之前成熟为两性体

The Journal of Cell Biology Pub Date : 2022-02-26 DOI: 10.1101/2022.02.26.482093

Jierui Zhao, Mai Thu Bui, Juncai Ma, Fabian Künzl, Lorenzo Picchianti, Juan Carlos De la Concepcion, Yixuan Chen, Sofia Petsangouraki, Azadeh Mohseni, M. García-León, Marta Salas Gomez, Caterina Giannini, Dubois Gwennogan, Roksolana Kobylinska, Marion Clavel, S. Schellmann, Y. Jaillais, J. Friml, Byungho Kang, Yasin F. Dagdas

{"title":"Plant autophagosomes mature into amphisomes prior to their delivery to the central vacuole","authors":"Jierui Zhao, Mai Thu Bui, Juncai Ma, Fabian Künzl, Lorenzo Picchianti, Juan Carlos De la Concepcion, Yixuan Chen, Sofia Petsangouraki, Azadeh Mohseni, M. García-León, Marta Salas Gomez, Caterina Giannini, Dubois Gwennogan, Roksolana Kobylinska, Marion Clavel, S. Schellmann, Y. Jaillais, J. Friml, Byungho Kang, Yasin F. Dagdas","doi":"10.1101/2022.02.26.482093","DOIUrl":"https://doi.org/10.1101/2022.02.26.482093","url":null,"abstract":"Autophagosomes are double-membraned vesicles that traffic harmful or unwanted cellular macromolecules to the vacuole for recycling. Although autophagosome biogenesis has been extensively studied, mechanisms of autophagosome maturation, i.e., delivery and fusion with the vacuole, remain largely unknown in plants. Here, we have identified an autophagy adaptor, CFS1, that directly interacts with the autophagosome marker ATG8 and localizes on both membranes of the autophagosome. Autophagosomes form normally in Arabidopsis thaliana cfs1 mutants, but their delivery to the vacuole is disrupted. CFS1’s function is evolutionarily conserved in plants as it also localizes to the autophagosomes and plays a role in autophagic flux in the liverwort Marchantia polymorpha. CFS1 regulates autophagic flux by connecting autophagosomes with the ESCRT-I component VPS23, leading to the formation of amphisomes. Disrupting the VPS23-CFS1 interaction affects autophagic flux and renders plants sensitive to starvation stress. Altogether, our results reveal a deeply conserved mechanism of vacuolar delivery in plants that is mediated by amphisomes.","PeriodicalId":343306,"journal":{"name":"The Journal of Cell Biology","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115122979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

MOSPD2 is an endoplasmic reticulum–lipid droplet tether functioning in LD homeostasis MOSPD2是一种内质网状脂滴系物，在LD稳态中起作用

The Journal of Cell Biology Pub Date : 2022-02-11 DOI: 10.1101/2022.02.11.479928

Mehdi Zouiouich, Thomas Di Mattia, Arthur Martinet, Julie Eichler, C. Wendling, Nario Tomishige, Erwan Grandgirard, Nicolas Fuggetta, C. Ramain, Giulia Mizzon, Calvin Dumesnil, Maxime Carpentier, B. Reina-San-Martin, C. Mathelin, Y. Schwab, A. Thiam, Toshihide Kobayashi, G. Drin, C. Tomasetto, F. Alpy

{"title":"MOSPD2 is an endoplasmic reticulum–lipid droplet tether functioning in LD homeostasis","authors":"Mehdi Zouiouich, Thomas Di Mattia, Arthur Martinet, Julie Eichler, C. Wendling, Nario Tomishige, Erwan Grandgirard, Nicolas Fuggetta, C. Ramain, Giulia Mizzon, Calvin Dumesnil, Maxime Carpentier, B. Reina-San-Martin, C. Mathelin, Y. Schwab, A. Thiam, Toshihide Kobayashi, G. Drin, C. Tomasetto, F. Alpy","doi":"10.1101/2022.02.11.479928","DOIUrl":"https://doi.org/10.1101/2022.02.11.479928","url":null,"abstract":"Membrane contact sites between organelles are organized by protein bridges. Among the components of these contacts, the VAP family comprises endoplasmic reticulum (ER)-anchored proteins, such as MOSPD2, functioning as major ER-organelle tethers. MOSPD2 distinguishes itself from the other members of the VAP family by the presence of a CRAL-TRIO domain. In this study, we show that MOSPD2 forms ER-LD contacts thanks to its CRAL-TRIO domain. MOSPD2 ensures the attachment of the ER to LDs through a direct protein-membrane interaction involving an amphipathic helix that has an affinity for lipid packing defects present at the surface of LDs. Remarkably, the absence of MOSPD2 markedly disturbs the assembly of lipid droplets. These data show that MOSPD2, in addition to being a general ER receptor for inter-organelle contacts, possesses an additional tethering activity and is specifically implicated in the biology of LDs via its CRAL-TRIO domain.","PeriodicalId":343306,"journal":{"name":"The Journal of Cell Biology","volume":"31 4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124927155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Assessing Gq-GPCR-induced human astrocyte reactivity using bioengineered neural organoids. 利用生物工程类神经器官评估gq - gpcr诱导的人类星形胶质细胞反应性。

IF 7.8

The Journal of Cell Biology Pub Date : 2022-02-09 DOI: 10.1083/jcb.202107135

Caroline Cvetkovic, Rajan Patel, Arya Shetty, Matthew K Hogan, Morgan Anderson, Nupur Basu, Samira Aghlara-Fotovat, Srivathsan Ramesh, Debosmita Sardar, Omid Veiseh, Michael E Ward, Benjamin Deneen, Philip J Horner, Robert Krencik

{"title":"Assessing Gq-GPCR-induced human astrocyte reactivity using bioengineered neural organoids.","authors":"Caroline Cvetkovic, Rajan Patel, Arya Shetty, Matthew K Hogan, Morgan Anderson, Nupur Basu, Samira Aghlara-Fotovat, Srivathsan Ramesh, Debosmita Sardar, Omid Veiseh, Michael E Ward, Benjamin Deneen, Philip J Horner, Robert Krencik","doi":"10.1083/jcb.202107135","DOIUrl":"https://doi.org/10.1083/jcb.202107135","url":null,"abstract":"Astrocyte reactivity can directly modulate nervous system function and immune responses during disease and injury. However, the consequence of human astrocyte reactivity in response to specific contexts and within neural networks is obscure. Here, we devised a straightforward bioengineered neural organoid culture approach entailing transcription factor-driven direct differentiation of neurons and astrocytes from human pluripotent stem cells combined with genetically encoded tools for dual cell-selective activation. This strategy revealed that Gq-GPCR activation via chemogenetics in astrocytes promotes a rise in intracellular calcium followed by induction of immediate early genes and thrombospondin 1. However, astrocytes also undergo NF-κB nuclear translocation and secretion of inflammatory proteins, correlating with a decreased evoked firing rate of cocultured optogenetic neurons in suboptimal conditions, without overt neurotoxicity. Altogether, this study clarifies the intrinsic reactivity of human astrocytes in response to targeting GPCRs and delivers a bioengineered approach for organoid-based disease modeling and preclinical drug testing.","PeriodicalId":343306,"journal":{"name":"The Journal of Cell Biology","volume":" ","pages":""},"PeriodicalIF":7.8,"publicationDate":"2022-02-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/44/69/JCB_202107135.PMC8842185.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39607886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

C-ferroptosis is an iron-dependent form of regulated cell death in cyanobacteria. c -铁下垂是蓝藻中受调节的细胞死亡的铁依赖形式。

IF 7.8

The Journal of Cell Biology Pub Date : 2022-02-07 Epub Date: 2021-11-24 DOI: 10.1083/jcb.201911005

Anabella Aguilera, Federico Berdun, Carlos Bartoli, Charlotte Steelheart, Matías Alegre, Hülya Bayir, Yulia Y Tyurina, Valerian E Kagan, Graciela Salerno, Gabriela Pagnussat, María Victoria Martin

{"title":"C-ferroptosis is an iron-dependent form of regulated cell death in cyanobacteria.","authors":"Anabella Aguilera, Federico Berdun, Carlos Bartoli, Charlotte Steelheart, Matías Alegre, Hülya Bayir, Yulia Y Tyurina, Valerian E Kagan, Graciela Salerno, Gabriela Pagnussat, María Victoria Martin","doi":"10.1083/jcb.201911005","DOIUrl":"https://doi.org/10.1083/jcb.201911005","url":null,"abstract":"Ferroptosis is an oxidative and iron-dependent form of regulated cell death (RCD) recently described in eukaryotic organisms like animals, plants, and parasites. Here, we report that a similar process takes place in the photosynthetic prokaryote Synechocystis sp. PCC 6803 in response to heat stress. After a heat shock, Synechocystis sp. PCC 6803 cells undergo a cell death pathway that can be suppressed by the canonical ferroptosis inhibitors, CPX, vitamin E, Fer-1, liproxstatin-1, glutathione (GSH), or ascorbic acid (AsA). Moreover, as described for eukaryotic ferroptosis, this pathway is characterized by an early depletion of the antioxidants GSH and AsA, and by lipid peroxidation. These results indicate that all of the hallmarks described for eukaryotic ferroptosis are conserved in photosynthetic prokaryotes and suggest that ferroptosis might be an ancient cell death program.","PeriodicalId":343306,"journal":{"name":"The Journal of Cell Biology","volume":" ","pages":""},"PeriodicalIF":7.8,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/98/d0/JCB_201911005.PMC8624678.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39766300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 21

Reconstitution of human atlastin fusion activity reveals autoinhibition by the C terminus. 重组人抗溶酶融合活性揭示了C末端的自抑制作用。

IF 7.8

The Journal of Cell Biology Pub Date : 2022-02-07 Epub Date: 2021-11-24 DOI: 10.1083/jcb.202107070

Daniel Crosby, Melissa R Mikolaj, Sarah B Nyenhuis, Samantha Bryce, Jenny E Hinshaw, Tina H Lee

{"title":"Reconstitution of human atlastin fusion activity reveals autoinhibition by the C terminus.","authors":"Daniel Crosby, Melissa R Mikolaj, Sarah B Nyenhuis, Samantha Bryce, Jenny E Hinshaw, Tina H Lee","doi":"10.1083/jcb.202107070","DOIUrl":"https://doi.org/10.1083/jcb.202107070","url":null,"abstract":"ER network formation depends on membrane fusion by the atlastin (ATL) GTPase. In humans, three paralogs are differentially expressed with divergent N- and C-terminal extensions, but their respective roles remain unknown. This is partly because, unlike Drosophila ATL, the fusion activity of human ATLs has not been reconstituted. Here, we report successful reconstitution of fusion activity by the human ATLs. Unexpectedly, the major splice isoforms of ATL1 and ATL2 are each autoinhibited, albeit to differing degrees. For the more strongly inhibited ATL2, autoinhibition mapped to a C-terminal α-helix is predicted to be continuous with an amphipathic helix required for fusion. Charge reversal of residues in the inhibitory domain strongly activated its fusion activity, and overexpression of this disinhibited version caused ER collapse. Neurons express an ATL2 splice isoform whose sequence differs in the inhibitory domain, and this form showed full fusion activity. These findings reveal autoinhibition and alternate splicing as regulators of atlastin-mediated ER fusion.","PeriodicalId":343306,"journal":{"name":"The Journal of Cell Biology","volume":" ","pages":""},"PeriodicalIF":7.8,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/77/cf/JCB_202107070.PMC8624677.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39766302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

CRISPR screens for lipid regulators reveal a role for ER-bound SNX13 in lysosomal cholesterol export. CRISPR筛选脂质调节因子揭示er结合SNX13在溶酶体胆固醇输出中的作用。

IF 7.8

The Journal of Cell Biology Pub Date : 2022-02-07 Epub Date: 2021-12-22 DOI: 10.1083/jcb.202105060

Albert Lu, Frank Hsieh, Bikal R Sharma, Sydney R Vaughn, Carlos Enrich, Suzanne R Pfeffer

引用次数: 0

Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation. 内膜上网状的REEP4促进核孔复合物的形成。

IF 7.8

The Journal of Cell Biology Pub Date : 2022-02-07 Epub Date: 2021-12-07 DOI: 10.1083/jcb.202101049

Banafsheh Golchoubian, Andreas Brunner, Helena Bragulat-Teixidor, Annett Neuner, Busra A Akarlar, Nurhan Ozlu, Anne-Lore Schlaitz

{"title":"Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation.","authors":"Banafsheh Golchoubian, Andreas Brunner, Helena Bragulat-Teixidor, Annett Neuner, Busra A Akarlar, Nurhan Ozlu, Anne-Lore Schlaitz","doi":"10.1083/jcb.202101049","DOIUrl":"https://doi.org/10.1083/jcb.202101049","url":null,"abstract":"Nuclear pore complexes (NPCs) are channels within the nuclear envelope that mediate nucleocytoplasmic transport. NPCs form within the closed nuclear envelope during interphase or assemble concomitantly with nuclear envelope reformation in late stages of mitosis. Both interphase and mitotic NPC biogenesis require coordination of protein complex assembly and membrane deformation. During early stages of mitotic NPC assembly, a seed for new NPCs is established on chromatin, yet the factors connecting the NPC seed to the membrane of the forming nuclear envelope are unknown. Here, we report that the reticulon homology domain protein REEP4 not only localizes to high-curvature membrane of the cytoplasmic endoplasmic reticulum but is also recruited to the inner nuclear membrane by the NPC biogenesis factor ELYS. This ELYS-recruited pool of REEP4 promotes NPC assembly and appears to be particularly important for NPC formation during mitosis. These findings suggest a role for REEP4 in coordinating nuclear envelope reformation with mitotic NPC biogenesis.","PeriodicalId":343306,"journal":{"name":"The Journal of Cell Biology","volume":" ","pages":""},"PeriodicalIF":7.8,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/5d/c1/JCB_202101049.PMC8656412.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39812571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Chromosome compartmentalization alterations in prostate cancer cell lines model disease progression. 前列腺癌细胞系的染色体区隔化改变模型疾病进展。

IF 7.8

The Journal of Cell Biology Pub Date : 2022-02-07 Epub Date: 2021-12-10 DOI: 10.1083/jcb.202104108

Rebeca San Martin, Priyojit Das, Renata Dos Reis Marques, Yang Xu, Justin M Roberts, Jacob T Sanders, Rosela Golloshi, Rachel Patton McCord

{"title":"Chromosome compartmentalization alterations in prostate cancer cell lines model disease progression.","authors":"Rebeca San Martin, Priyojit Das, Renata Dos Reis Marques, Yang Xu, Justin M Roberts, Jacob T Sanders, Rosela Golloshi, Rachel Patton McCord","doi":"10.1083/jcb.202104108","DOIUrl":"https://doi.org/10.1083/jcb.202104108","url":null,"abstract":"Prostate cancer aggressiveness and metastatic potential are influenced by gene expression and genomic aberrations, features that can be influenced by the 3D structure of chromosomes inside the nucleus. Using chromosome conformation capture (Hi-C), we conducted a systematic genome architecture comparison on a cohort of cell lines that model prostate cancer progression, from normal epithelium to bone metastasis. We describe spatial compartment identity (A-open versus B-closed) changes with progression in these cell lines and their relation to gene expression changes in both cell lines and patient samples. In particular, 48 gene clusters switch from the B to the A compartment, including androgen receptor, WNT5A, and CDK14. These switches are accompanied by changes in the structure, size, and boundaries of topologically associating domains (TADs). Further, compartment changes in chromosome 21 are exacerbated with progression and may explain, in part, the genesis of the TMPRSS2-ERG translocation. These results suggest that discrete 3D genome structure changes play a deleterious role in prostate cancer progression. .","PeriodicalId":343306,"journal":{"name":"The Journal of Cell Biology","volume":" ","pages":""},"PeriodicalIF":7.8,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/2f/JCB_202104108.PMC8669499.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39711300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

Live imaging of transcription sites using an elongating RNA polymerase II-specific probe. 使用延长RNA聚合酶ii特异性探针对转录位点进行实时成像。

IF 7.8

The Journal of Cell Biology Pub Date : 2022-02-07 Epub Date: 2021-12-02 DOI: 10.1083/jcb.202104134

Satoshi Uchino, Yuma Ito, Yuko Sato, Tetsuya Handa, Yasuyuki Ohkawa, Makio Tokunaga, Hiroshi Kimura

{"title":"Live imaging of transcription sites using an elongating RNA polymerase II-specific probe.","authors":"Satoshi Uchino, Yuma Ito, Yuko Sato, Tetsuya Handa, Yasuyuki Ohkawa, Makio Tokunaga, Hiroshi Kimura","doi":"10.1083/jcb.202104134","DOIUrl":"https://doi.org/10.1083/jcb.202104134","url":null,"abstract":"In eukaryotic nuclei, most genes are transcribed by RNA polymerase II (RNAP2), whose regulation is a key to understanding the genome and cell function. RNAP2 has a long heptapeptide repeat (Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7), and Ser2 is phosphorylated on an elongation form. To detect RNAP2 Ser2 phosphorylation (RNAP2 Ser2ph) in living cells, we developed a genetically encoded modification-specific intracellular antibody (mintbody) probe. The RNAP2 Ser2ph-mintbody exhibited numerous foci, possibly representing transcription \"factories,\" and foci were diminished during mitosis and in a Ser2 kinase inhibitor. An in vitro binding assay using phosphopeptides confirmed the mintbody's specificity. RNAP2 Ser2ph-mintbody foci were colocalized with proteins associated with elongating RNAP2 compared with factors involved in the initiation. These results support the view that mintbody localization represents the sites of RNAP2 Ser2ph in living cells. RNAP2 Ser2ph-mintbody foci showed constrained diffusional motion like chromatin, but they were more mobile than DNA replication domains and p300-enriched foci, suggesting that the elongating RNAP2 complexes are separated from more confined chromatin domains.","PeriodicalId":343306,"journal":{"name":"The Journal of Cell Biology","volume":" ","pages":""},"PeriodicalIF":7.8,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8647360/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39937204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12

Atg39 links and deforms the outer and inner nuclear membranes in selective autophagy of the nucleus. 在核的选择性自噬中，Atg39连接并变形核膜的内外膜。

IF 7.8

The Journal of Cell Biology Pub Date : 2022-02-07 Epub Date: 2022-01-21 DOI: 10.1083/jcb.202103178

Keisuke Mochida, Toshifumi Otani, Yuto Katsumata, Hiromi Kirisako, Chika Kakuta, Tetsuya Kotani, Hitoshi Nakatogawa

{"title":"Atg39 links and deforms the outer and inner nuclear membranes in selective autophagy of the nucleus.","authors":"Keisuke Mochida, Toshifumi Otani, Yuto Katsumata, Hiromi Kirisako, Chika Kakuta, Tetsuya Kotani, Hitoshi Nakatogawa","doi":"10.1083/jcb.202103178","DOIUrl":"https://doi.org/10.1083/jcb.202103178","url":null,"abstract":"In selective autophagy of the nucleus (hereafter nucleophagy), nucleus-derived double-membrane vesicles (NDVs) are formed, sequestered within autophagosomes, and delivered to lysosomes or vacuoles for degradation. In Saccharomyces cerevisiae, the nuclear envelope (NE) protein Atg39 acts as a nucleophagy receptor, which interacts with Atg8 to target NDVs to the forming autophagosomal membranes. In this study, we revealed that Atg39 is anchored to the outer nuclear membrane via its transmembrane domain and also associated with the inner nuclear membrane via membrane-binding amphipathic helices (APHs) in its perinuclear space region, thereby linking these membranes. We also revealed that autophagosome formation-coupled Atg39 crowding causes the NE to protrude toward the cytoplasm, and the tips of the protrusions are pinched off to generate NDVs. The APHs of Atg39 are crucial for Atg39 crowding in the NE and subsequent NE protrusion. These findings suggest that the nucleophagy receptor Atg39 plays pivotal roles in NE deformation during the generation of NDVs to be degraded by nucleophagy.","PeriodicalId":343306,"journal":{"name":"The Journal of Cell Biology","volume":" ","pages":""},"PeriodicalIF":7.8,"publicationDate":"2022-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d2/8d/JCB_202103178.PMC8789198.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39707475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0