THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)最新文献_第3页

Beneficial Impact of Folic Acid Against Eco-Physiological Toxicity Induced By Potassium Bromate In Male Rats 叶酸对雄性大鼠溴酸钾生态生理毒性的有益影响

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20230115082324

{"title":"Beneficial Impact of Folic Acid Against Eco-Physiological Toxicity Induced By Potassium Bromate In Male Rats","authors":"","doi":"10.5455/egysebz.20230115082324","DOIUrl":"https://doi.org/10.5455/egysebz.20230115082324","url":null,"abstract":"Potassium bromate (KBrO3) used in both the food and cosmetics industry, and a drinking water disinfection. Strong oxidizer KBrO3 has been employed in the milling of flour. It is a substance that is nephrotoxic and carcinogenic. Acute renal failure and the potential for injury to the reproductive systems were both associated with the therapy KBrO3. Food naturally contains folic acid, especially dark green leafy vegetables. The study aimed to investigation the effects of folic acid supplementation on the KBrO3 toxicity. Animals were randomly divided into four groups: group I control; group II received daily potassium bromate administration (200 mg/kg bw); group III received daily folic acid administration (40 mg/kg); and group IV received daily administration of 200 mg/kg potassium bromate and 40 mg/kg folic acid, respectively. Blood samples were examined for levels of creatine kinase (CK-MB), troponin, lactate dehydrogenase, creatinine, urea, luteinizing hormone (LH), testosterone, and follicle-stimulating hormone (FSH). The experimental animals administered by PB had a substantial increase in CK-MB, troponin, LDH, creatinine, urea, and FSH whereas all experimental rats had a drop in LH and testosterone levels in accordance with the toxicity of potassium bromate. The administration of folic acid had a protective effect on the group of rats treated concurrently with potassium bromate and folic acid. . The heart, kidney, and testis tissues underwent histological examinations, which supported the biochemical findings that the heart, kidney, and testis of KBrO3-treated animals had experienced significant extensive damage. In conclusion, these results show that KBrO3 has serious damaging effects on experimental animals and therefore, its use should be avoided. These dangerous effects should stop its use in human being.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116100701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Composing solutions for spin-column based RNA purification from eukaryotic cells: An educational research article 真核细胞中基于自旋柱的RNA纯化:一篇教育研究文章

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220517120328

M. Abdelfattah, Mohamed R. Elnagar, M. Ibrahim, M. Albert, Maya Talal, Reham Helwa

{"title":"Composing solutions for spin-column based RNA purification from eukaryotic cells: An educational research article","authors":"M. Abdelfattah, Mohamed R. Elnagar, M. Ibrahim, M. Albert, Maya Talal, Reham Helwa","doi":"10.5455/egysebz.20220517120328","DOIUrl":"https://doi.org/10.5455/egysebz.20220517120328","url":null,"abstract":"Twenty years ago, working with molecular biology research was very costly worldwide. Later on, after distributing the know-how of many reagents, it becomes more feasible to work in this field with reasonable costs. In addition, the optimization of large-scale production of enzymes, kits, lab consumables, and reagents made a big revolution in research and diagnostics. So, having affordable technologies in any molecular biology laboratory is an aim itself, especially in developing countries. After Covid-19 crisis, the need of molecular biology reagents is in surge. However, consumables’ prices, delayed orders, and shortage are the prominent issues after the pandemic. Thus, in the present study, homemade solutions were investigated for RNA purification from HepG2 and huh7 cells. The RNA was successfully purified by the homemade solutions and amplified using qRT-PCR. However DNA contamination was encountered which could be eliminated simply by DNase I digestion or designing proper primers in exon-exon junction for RT-PCR, in addition to precise normalization strategy.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124899809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural and biochemical studies on the potential effects of Moringa oleifera seed oil against nephrotoxicity induced by sofosbuvir in albino rats 辣木籽油抗索非布韦致白化大鼠肾毒性的超微结构及生化研究

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20221105123510

H. Mahran, Y. Okdah, Aisha A. Zaky, Samah M. Arisha

{"title":"Ultrastructural and biochemical studies on the potential effects of Moringa oleifera seed oil against nephrotoxicity induced by sofosbuvir in albino rats","authors":"H. Mahran, Y. Okdah, Aisha A. Zaky, Samah M. Arisha","doi":"10.5455/egysebz.20221105123510","DOIUrl":"https://doi.org/10.5455/egysebz.20221105123510","url":null,"abstract":"The hepatitis C virus (HCV) causes both acute and chronic hepatitis which spread worldwide and accompanied with severe complications in long period of injury. Sofosbuvir (sofo) is a new drug for HCV treatment. Although it has an effective potential in reducing HCV load, its use was accompanied by some side effects. Moringa oleifera (MO) seed oil is natural food-supplemented oil has many pharmaceutical properties which give the plant the ability to reduce or improve the side effects of many insults. This work aimed to determine the possible protective effects of MO seed oil against sofo-nephrotoxicity. Four experimental groups were used; the control group, MO oil group was orally given MO seed oil (2ml/kg/day), sofo group was orally given sofo (36mg/kg/day) and sofo and MO oil group was orally administered sofo, then after 2 hours they were given MO oil. The rats received all doses daily for eight weeks. Twenty four hours following the last injection, rats were anesthetized, sacrificed, the two kidneys were quickly removed and the blood samples were withdrawn for complete blood count (CBC) and determination of the activity of superoxide dismutase (SOD) and the level of glutathione (GSH). Sofosbuvir induced marked ultrastructural changes and significant decrease in SOD activity and GSH level. In addition, CBC of the same groups showed significant decrease in the mean corpuscular value and the mean corpuscular hemoglobin and significant increase in red blood cells count. On the other hand, co-treatment with MO seed oil efficiency improved sofo-induced nephrotoxicity which was evident from the disappearance of most of the ultrastructural changes and improving SOD activity and GSH level. Conclusions: Moringa oleifera seed oil may improve the ultrastructural and biochemical changes associated with nephrotoxicity induced by sofo due to its antioxidant and anti-inflammatory constituents.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"123 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130216025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Gymnema sylvestre leaf extract on hepatotoxicity induced by cisplatin in rats 刺五加叶提取物对顺铂诱导的大鼠肝毒性的作用

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220820103856

Doaa Ibrahim

引用次数: 0

Residual activity of powders and oils of Pimpinella anisum, Citrus aurantium and Origanum majorana as grain protectants against Callosobruchus maculatus (F.) and Sitphilus oryzae (L.) 茴香、金柑和大黄牛粉和油对斑点斑孔口疮和米褐虱的残留活性研究

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190718053447

T. Ismail, N. Hassan, G. Zayed

{"title":"Residual activity of powders and oils of Pimpinella anisum, Citrus aurantium and Origanum majorana as grain protectants against Callosobruchus maculatus (F.) and Sitphilus oryzae (L.)","authors":"T. Ismail, N. Hassan, G. Zayed","doi":"10.5455/egysebz.20190718053447","DOIUrl":"https://doi.org/10.5455/egysebz.20190718053447","url":null,"abstract":"The cowpea weevil, Callosobruchus maculatus (F.) and the rice weevil, Sitphilus oryzae (L.) are destructive insects attacking cowpea seeds and rice grains causing weight loss and reduced nutrition values. Laboratory experiments were conducted to evaluate the insecticidal toxicity of three plant oils and powders of anise (Pimpinella anisum), Bitter orange (Citrus aurantium) and Sweet marjoram (Origanum majorana ) as grain protectants on the mortality of C. maculatus and S. oryzae. The results showed that marjoram and anise in oil forms were highly toxic on C. maculatus with LC50 values of 0.69 and 1.09 ppm, respectively and on S. oryzae with LC50 values of 0.533 and 0.515 ppm, respectively. Furthermore, the residual toxicity of the tested materials was evaluated through three months of storage against adult and progeny of the two insects. The tested materials significantly increased the mortality percentages and reduced the fecundity of the two insects depending on the dosage, the exposure time and storage periods compared with control. Malathion had the highest effect against both insects compared to the plant products. The plant oils showed more efficacy than the powders against the two insects in initial time storage while, the plant powders were more effective at the end of storage periods. The identification of chemical composition of tested plant oils was done by using GC-MS analysis. Considering the results of the current study, it could be suggested that the three plant oils and powders especially, anise and marjoram may have potential to be used as an alternative to synthetic insecticides in an integrated pest management program for protecting stored grains.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129779220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison between Platelet Activation Factor and Pentoxifylline on Sperm in Asthenozoospermia Cases. 血小板活化因子与己酮茶碱对弱精子症患者精子影响的比较。

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20220313032140

Mohamed Saber, A. Shafey, Mostafa Alabedeen, A. Massoud, M. Elwan

引用次数: 1

Adverse effects of therapeutic doses of sofosbuvir and ribavirin on the liver of the developing mice embryos 索非布韦和利巴韦林治疗剂量对发育中的小鼠胚胎肝脏的不良影响

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20210706095316

Esraa A Shahat, H. Shabaka, I. Zakaria, Suzan Ahmed

{"title":"Adverse effects of therapeutic doses of sofosbuvir and ribavirin on the liver of the developing mice embryos","authors":"Esraa A Shahat, H. Shabaka, I. Zakaria, Suzan Ahmed","doi":"10.5455/egysebz.20210706095316","DOIUrl":"https://doi.org/10.5455/egysebz.20210706095316","url":null,"abstract":"The aim of this study was to investigate the adverse effect of Sofosbuvir (SFV) and / or Ribavirin (RBV) on developing mice embryos in order to determine the morphological and the liver tissue alterations of mice fetuses. The pregnant mice were divided into eight groups: 1) control group (GI); 2) SFV 1.64 mg/mouse/day (GII); 3) SFV 4.93 mg/mouse/day (GIII); 4) RBV 0.2065 mg/mouse/day (GIV); 5) RBV 0.856 mg/mouse/day (GV); 6) SFV 1.64 mg/mouse/day and RBV 0.856 mg/mouse/day (GVI); 7) SFV 4.93 mg/mouse/day and RBV 0.856 mg/mouse/day (GVII) and 8) SFV 1.64 mg/mouse/day and RBV 0.2065 mg/mouse/day (GVIII). Maternal exposure of both drugs SFV and/or RBV affected the body weight, number of fetuses and presence of resorption sites. A variety of morphological abnormalities were detected in all groups demonstrating several craniofacial malformations and conspicuous congenital malformations. The most pronounced effects in (GV) were the detection of exencephaly and facial condition. The liver of the maternally treated fetuses of (GII) and (GIV) revealed necrotic hepatocytes with vacuolated cytoplasm. While (GIII) and (GV) revealed hepatocytes degeneration, damaged endothelial cells lining the central veins and the presence of activated Kupffer cells.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126831396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The protective effect of Nigella sativa oil against reproductive toxicity, hormonal alterations, and oxidative damage induced by Sertraline in male rats 黑草油对舍曲林诱导的雄性大鼠生殖毒性、激素改变和氧化损伤的保护作用

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190325113108

Dawlat A Sayed

引用次数: 1

Protective effect of Fragaria ananassa againststreptozotocin- induced diabetes in rats 黄芪对链脲佐菌素诱导大鼠糖尿病的保护作用

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) Pub Date : 1900-01-01 DOI: 10.5455/EGYSEBZ.20190415100613

E. Shaheen

{"title":"Protective effect of Fragaria ananassa against\u0000streptozotocin- induced diabetes in rats","authors":"E. Shaheen","doi":"10.5455/EGYSEBZ.20190415100613","DOIUrl":"https://doi.org/10.5455/EGYSEBZ.20190415100613","url":null,"abstract":"The present study aimed to clarify the effect of strawberry (Fragaria ananassa) leaves extract on some physiological parameters of streptozotocin-induced diabetes in rats. Diabetic rats were orally treated daily with three doses (50, 100 and 200 mg/kg) of strawberry leaves extract for 30 days. Diabetic rats exhibited significant increases in plasma glucose, triglycerides, total cholesterol, LDL-cholesterol, malondialdehyde levels in addition to the activities of catalase, alanine aminotransaminase (ALT) and aspartate aminotransaminase (AST) as compared to the control. On the other side, significant decreases in insulin, total protein, albumin, globulin, A/G ratio, HDL- cholesterol and superoxide dismutase levels were recorded. Treatment of diabetic rats with strawberry leaves extract significantly decreased plasma glucose, ALT, AST, triglycerides, total cholesterol, LDL- cholesterol, malondialdehyde and significantly increased insulin, total protein, albumin, globulin, A/G ratio, HDL- cholesterol and superoxide dismutase levels as compared to the diabetic rats. Therefore, the present data declared the efficiency of strawberry leaves extract for relieving the metabolic hazard of diabetics. Furthermore, the treatment with 200 mg/kg b.w. was more effective than treatment with 50 mg or 100mg/ kg b.w. the extract.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"189 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114555059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Haemogregarines infecting reptiles in Egypt1) Blood and merogenics stages of Haemogregarine sp. infecting the skink Scincus scincus in Egypt 埃及爬行动物感染血毒杆菌(Haemogregarine)感染埃及石龙的血毒杆菌属(Haemogregarine)的血液和新生阶段

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology) Pub Date : 1900-01-01 DOI: 10.5455/egysebz.20190619042106

Hewaydah E. Abou Shafeey, H. Mohamadain, Rewaida Gaber, Nazera Emara

{"title":"Haemogregarines infecting reptiles in Egypt\u00001) Blood and merogenics stages of Haemogregarine sp. infecting the skink Scincus scincus in Egypt","authors":"Hewaydah E. Abou Shafeey, H. Mohamadain, Rewaida Gaber, Nazera Emara","doi":"10.5455/egysebz.20190619042106","DOIUrl":"https://doi.org/10.5455/egysebz.20190619042106","url":null,"abstract":"In the present study, 25 individual male and females of the skink Scincus scincus were collected and examined for the prevalence of haemogregaarine infection. Peripheral blood smears and thin blood films were prepared and microscopically examined. Eight animals were found to be infected with an infection rate reaching 32%. Parasitaemia was changed from low level to the highest one reaching 25-40%. Intracellular blood stages of different morphological and morphometric characteristics were only parasitize the blood erythrocytes. None of leucocytes were found to be infected. Infected blood cells were completely changed, hypertrophied; their nuclei were displaced and deformed. Single and double parasite individual were found to harbor the same cell. All parasites were enclosed in parasitophorous vacuoles. These vacuoles were sometimes clear, wide and easily observed. Some free extracellular blood stages were rarely observed. Different developmental stages of meronts were observed in liver parenchyma cells which considered as the main site of infection and extends to infect the spleen in heavily infected cases. Two types of meronts were clearly identified, the small form (micromeronts) with small number of nuclei that measured 16.0±0.5 × 10.5±0.7 μm and produce large merozoites (macromerozoites), the large form (macromeronts) with numerous nuclei that measured 25.4±0.6 × 17.8±0.6 μm and produce smaller and more numerous merozoites.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"108 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115070385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3