H. Elbrense, A. Seif, Ibrahem B. Helal, Mohamed M. Shamseldean, Iman Elhussieny
{"title":"Foraging behaviour alterations of Culex quinquefasciatus Say (Diptera: Culicidae) mosquito larvae induced by Romanomermis iyengari Welch (Nematoda: Mermithidae) parasitism","authors":"H. Elbrense, A. Seif, Ibrahem B. Helal, Mohamed M. Shamseldean, Iman Elhussieny","doi":"10.5455/egysebz.20220301085416","DOIUrl":"https://doi.org/10.5455/egysebz.20220301085416","url":null,"abstract":"","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133779449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Genes controlling appetite, gut emptying rate and metabolism in the early stage of Rotenone-induced semi-Parkinsonism in Drosophila melanogaster","authors":"Mai Fathy, Mohamed Shaheen, S. Kholy","doi":"10.5455/egysebz.20220811020747","DOIUrl":"https://doi.org/10.5455/egysebz.20220811020747","url":null,"abstract":"Parkinson’s disease (PD) is one of the most serious neurodegenerative diseases. PD is characterized by progressive death of dopamine producing cells in Substantia nigra pars compacta within human brain causing serious locomotor defects such as tremor as well as non-motor symptoms including metabolic deficiency and constipation. Despite the plethora of information regarding the underlying mechanisms related to locomotion impairment, signal pathways/genes involved in metabolic deficiency and constipation remain unclear. This study aims to explore the most important key genes involved in metabolic syndrome and constipation observed in PD patients. To achieve this goal, real-time polymerase chain reaction (RT PCR) of a group of genes supposed in previous findings to have a role in these syndromes in rotenone - induced PD Drosophila model was performed. One -day- old Canton S flies were fed on filter paper saturated with rotenone glucose solution(5mM rotenone in 10% glucose) at 25 ̊C for 24h.Thereafter, we focus on genes whose up-regulated expression level for all subsequent experiment. Next, metabolic parameters (total protein, lipid and carbohydrates), rate of food consumption and defecation in flies that’s knocking down in the corresponding genes were evaluated. Results revealed that feeding rate decreased when CG17544 and CG16848 genes were up-regulated; consequently those flies had reduced body weight. Meanwhile, knocking down CG16848 and CG12913. This study provide evidence that using the human homologues of these Drosophila genes for molecular docking of new pharmacotherapeutics may help to enhance the quality of life of PD patients through improving metabolism as well as defecation.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"158 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124711088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Description of two novel Echinochasmus species (Digenea: Echinostomatidae) from the squacco heron Ardeola ralloides (Aves: Ardeidae) in Egypt: A light and scanning electron microscopic study","authors":"R. Mansour, Amina Elfatah","doi":"10.5455/egysebz.20190908034421","DOIUrl":"https://doi.org/10.5455/egysebz.20190908034421","url":null,"abstract":"Adult forms of two new echinostomatids were recovered from small intestine of six out of ten squacco herons, Ardeola ralloides collected from March to July 2019 from Giza governorate, Egypt. These small-sized parasites had a single row of collar spines with middle dorsal interruption, vitellaria reaching the level of ventral sucker and attributed to genus Echinochasmus, namely, E. ralloides n. sp. (prevalence 40%) and E. rawashi n. sp. (prevalence 20%). The morphometric features and differential indices of two species were measured, compared and described using light and scanning electron microscopy (SEM). The body of E. ralloides n. sp. measured 959.2-1090.6 × 264.3-311.1 μm (L × MW). It was provided with 26 collar spines (9 dorsal, 2 lateral and 2 angle on each side of the dorsal collar interruption), lacked of tegumental spines except for small areas in lower left and lateral right margins of ventral sucker, and characterized by presence of many minute pores of 3.1-3.8 μm on dorsal surface of hindbody. The body of E. rawashi n. sp. measured 638.8-1173.5 × 138.8-207.7 μm (L × MW), provided with 24 collar spines (7 dorsal, 2 lateral and 2 angle on each side of the dorsal collar interruption) and had scale-like tegumental spines distributed on whole ventral surface and anterior 1/4 of dorsal surface of body, ended by up to 13 digit-like structures and measured 2.1-2.5 × 4.4-4.8 μm.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"59 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130464321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Low- versus High-water level effect on subtropical benthic freshwater meiofauna","authors":"Ahmed Abada","doi":"10.5455/egysebz.20221229092731","DOIUrl":"https://doi.org/10.5455/egysebz.20221229092731","url":null,"abstract":"Little is known about meiofaunal responses to Low- versus High-Water-Level (L- vs HWL) particularly in the subtropics. Meiofaunal reaction was evaluated in three locations (deeply-different) in two man-made freshwater channels in (Rowaina and Shalma) Northern Nile Egyptian delta: 50, 100, & 150, and 900, 950, & 1000 metres distances from the sluice, respectively. LWL and HWL were in January and July 2019 respectively. Eight vs ten and nine vs eleven major taxa were noted in Rowaina H- vs LWL and Shalma L- vs HWL, respectively. Taxa had different susceptibilities to L- vs HWL. Nematode, hydracarina, and oligochaetes dominated in L- and HWL, (Shalma), and in HWL (Rowaina). Whereas Nematoda, Copepod, and Oligochaeta prevailed in LWL (Rowaina). Oligochaeta biomass was generally high in LWL and HWL. Hydracarina in LWL, and Nematode & Copepod in HWL had low biomasses in Rowaina and Shalma respectively. Abundances and biomasses were significantly different within and among channels. Environmental variables were significantly different only between water levels. It could be concluded that L- vs HWL has both direct and indirect (variabilities in environmental factors) impacts on meiofauna. Channel width & morphology, may impose extra influences. This study may enhance invertebrate surveys with hydrologically different freshwater bodies.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124957365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Siham B. Salem, S. Naggar, Ramadan M. Kandyel, M. Sharkawy
{"title":"Histological and immunohistochemical studies on the effect of zinc oxide nanoparticles on the cerebellum of newborn and adult rats and the possible protective role of EDTA","authors":"Siham B. Salem, S. Naggar, Ramadan M. Kandyel, M. Sharkawy","doi":"10.5455/egysebz.20221222014955","DOIUrl":"https://doi.org/10.5455/egysebz.20221222014955","url":null,"abstract":"Zinc oxide nanoparticles (ZnO NPs) are involved in different applications such as nutrition, while, it causes damage to different organs of the body including the brain. This study aimed to compare between the effect of ZnO NPs on newborn and adult rats´ cerebellum and to evaluate the protective role of Ethylenediaminetetraacetic acid (EDTA) against ZnO NPs toxicity. Thirty-two newborn (NBM)and thirty-two adult male (ADM) Sprague Dawley rats are used. Four groups (n = 8) of each are divided as following; Gp1: is served as control, Gp2: injected intraperitoneal (IP) with ZnO NPs (3 g/ kg). Gp3: injected IP with EDTA (500 mg/ kg) for 7 days. Gp4: injected with ZnO NPs and treated with EDTA as in Gp3. Brain tissue is prepared for biochemical and histopathological investigations. Newborn and adult rats injected with ZnO NPs showed degeneration of purkinje and granular cells in cerebellum tissue confirmed with high expression of caspase-3, while, treatment with EDTA post ZnO NPs showed lightly improvement. The body weights and relative brain weights is decrease in all the rats treated with ZnO NPs. In addition, our current study proved that the effect of ZnO NPs on ADM rats is more than on the NBM ones. Collectively, ZnO NPs injection caused damage of the cerebellum in newborn and adult rats, however, the treatment with EDTA stopped somewhat this damage.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121123179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. Garawani, E. Khallaf, Alla A. Alne na ei, R. G. Elgendy
{"title":"RNA/DNA ratio and expression of GHR and IGF-1 hormones: A study on seasonal variation effect on growth characterization of male Oreochromis niloticus","authors":"I. Garawani, E. Khallaf, Alla A. Alne na ei, R. G. Elgendy","doi":"10.5455/egysebz.20190715025948","DOIUrl":"https://doi.org/10.5455/egysebz.20190715025948","url":null,"abstract":"Nile tilapia (Oreochromis niloticus) is considered as one of the most economical and popular aquaculture species. One of the main concerns in tilapia aquaculture, as well as in the wild, is growth, because of its importance for the fish population, stock assessment and their production in aquaculture. Accordingly, this study was initiated to characterize growth on molecular basis. In the same respect, RNA/DNA ratio and GHR-1/IGF-1as growth-related genes are known to be involved in weight gain alterations. To assess the correlation between growth, biochemical and hormonal conditions of fish during summer and winter. Adult male O. niloticus (100 - 250gm) were collected from Bahr Shebeen Canal, for such investigation. The results revealed that the GHR-1 and IGF-1 mRNA were directly proportional to age, length and weight. However, they were up regulated in summer than in winter. However, RNA/DNA ratio gave an observable positive correlation with age and length only with higher values in winter. In conclusion, this study supports the use of GHR-1/IGF-1as reliable indices of growth and condition of O. niloticus in their natural habitat in addition to RNA/DNA ratio assessment.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"61 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131113730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Rationalized manner for controlling Sitophilus oryzae (L.) and Rhyzopertha dominica (F.) using spinosad and ivermectin biocides.","authors":"T. Ismail, Mostafa E. E. Elebiary","doi":"10.5455/egysebz.20190708120158","DOIUrl":"https://doi.org/10.5455/egysebz.20190708120158","url":null,"abstract":"In this study, we evaluated the efficiency of two biocides, spinosad and ivermectin against two important stored grain insects, Sitophilus oryzae (L.) and Rhyzopertha dominica (F.) by using bioassay method, mixing with medium. Obtained results demonstrated that ivermectin had the highest effect on adult mortality for both insects using complete dose. Rates of spinosad or ivermectin from 50-150 ppm completely prevented emergence of adults of both insects. R. dominica was more susceptible to biocides tested than S. oryzae. Half-dose of spinosad or ivermectin nearly produced the same effect with complete dose on S. oryzae and R. dominica, while the one-third dose was less effect. The current results encourage using the half-dose instead of the full dose of both insecticides for controlling the tested insects to rationalize the insecticide application and to minimize their expected hazards.","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"160 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133565514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nabila Desouki, Basyouny Elfotouh, Soha Okba, Buthina Alshammari
{"title":"Role of milk thistle (Silybum marianum) oil in the improvement of carbon tetrachloride-induced liver fibrosis and cirrhosis in albino mice","authors":"Nabila Desouki, Basyouny Elfotouh, Soha Okba, Buthina Alshammari","doi":"10.5455/egysebz.20220404091009","DOIUrl":"https://doi.org/10.5455/egysebz.20220404091009","url":null,"abstract":"","PeriodicalId":342785,"journal":{"name":"THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Zoology)","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133001217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}