Oil Crop Science最新文献

{"title":"Ameliorative effect of walnut oil against cognitive impairment in alzheimers type dementia in rodent","authors":"Congcong Ma ,&nbsp;Li Zhang ,&nbsp;Lei Wang ,&nbsp;Qingde Huang ,&nbsp;Qianchun Deng ,&nbsp;Fenghong Huang ,&nbsp;Jiqu Xu","doi":"10.1016/j.ocsci.2024.09.003","DOIUrl":"10.1016/j.ocsci.2024.09.003","url":null,"abstract":"<div><div>Walnut oil (WO), known for abundant polyunsaturated fatty acids and an array of bioactive substances such as tocopherols, phytosterols, squalene, melatonin, and polyphenols, which is endowed with numerous health advantages. The primary objective of this research was to ascertain the impact of WO on cognitive deficits in learning and memory impairment mice caused by scopolamine (SCOP). The Morris water maze and the step-down avoidance test were utilized to assess the memory and learning capabilities. WO notably counteracted the detrimental effects of SCOP on learning and memory in the Morris water maze, as indicated by a reduction in escape latency and swimming distance. Likewise, WO administration led to a notably reduced number of errors in training trial and an increased latency in testing trial when compared to the SCOP group in the step-down avoidance test,. Moreover, WO activated the cholinergic system of the brain by upregulating choline acetyltransferase activity and reducing acetylcholinesterase activity. These results suggest that WO has the potential to protect against memory decline in mice, offering a promising strategy for the prevention of memory-related disorders.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 234-239"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142662057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural variations in oil crops: Types, and roles on domestication and breeding","authors":"Xiaobo Cui,&nbsp;Miao Yao,&nbsp;Meili Xie,&nbsp;Ming Hu,&nbsp;Shengyi Liu,&nbsp;Lijiang Liu,&nbsp;Chaobo Tong","doi":"10.1016/j.ocsci.2024.09.002","DOIUrl":"10.1016/j.ocsci.2024.09.002","url":null,"abstract":"<div><div>Structural variations (SVs), a newly discovered genetic variation, have gained increasing recognition for their importance, yet much about them remains unknown. With the completion of whole-genome sequencing projects in oil crops, more SVs have been identified, revealing their types, genomic distribution, and characteristics. These findings have demonstrated the crucial roles of SVs in regulating gene expression, driving trait innovation, facilitating domestication, making this an opportune time for a systematic review. We summarized the progress of SV-related studies in oil crops, focusing on the types of SVs and their mechanisms of occurrence, the strategies and methods for SV detection, and the SVs identified in oil crops such as rapeseed, soybean, peanut, and sesame. The various types of SVs, such as presence-absence variations (PAVs), copy number variations (CNVs), and homeologous exchanges (HEs), have been shown. Along with their genomic characterization, their roles in crop domestication and breeding, and regulatory impact on gene expression and agronomic traits have also been demonstrated. This review will provide an overview of the SV research process in oil crops, enabling researchers to quickly understand key information and apply this knowledge in future studies and crop breeding.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 240-246"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New insights into the combined effects of geographical origin, cultivar and crop season on the physicochemical characteristics of Moroccan olive oils produced in northern Morocco. A comparative study","authors":"Noura Issaoui ,&nbsp;Inmaculada Olivares ,&nbsp;Amar Habsaoui ,&nbsp;Mohamed Ebn Touhami ,&nbsp;Adil Khtira ,&nbsp;El Hassan Sakar ,&nbsp;Sebastián Sánchez","doi":"10.1016/j.ocsci.2024.11.001","DOIUrl":"10.1016/j.ocsci.2024.11.001","url":null,"abstract":"<div><div>The Tangier-Tetouan-Al Hoceima (TTA) region is one of the main olive oil producing regions in Morocco. Little is devoted to characterize olive oil physicochemical traits from TTA hence the originality of this study. This aimed at investigating variation in olive oil quality produced from three Moroccan cultivars ‘Moroccan Picholine’, ‘Menara’, and ‘Haouzia’ and their blends. Sampling was performed in five provinces fromTTA (Northern Morocco) during four consecutive crop-seasons (2018–2021) considering three extraction technologies (ET): traditional discontinuous press system (SP) and continuous extraction systems including decanter of three outlets (3O) and decanter of two outlets (2O). Physicochemical measurements consisted of routinely quality parameters namely free acidity (FA), peroxide value (PV), UV absorption parameters (<em>K</em>₂₃₂, <em>K</em>₂₇₀, and <em>ΔK</em>), chlorophylls (Chl) and carotenoids (Car) contents, total phenolic compounds (TPC) and oxidative stability (OS). Crop season showed its superiority impacts on <em>K</em>₂₃₂, OS, TPC, Chl, and OS. While cultivar was the main variability source in both PV and <em>K</em>₂₇₀, and FA was mainly determined by ET. Important variations (<em>p</em> ​&lt; ​0.05) were reported among crop seasons and locations due to pedoclimatic differences. ‘Menara’ and ‘Haouzia’ had higher pigments content, TPC, and OS, and the blends displayed low pigments concentration, TPC, and OS. Expectedly, continuous ET (2O and 3O) had the greatest values of pigments content, TPC, and OS as revealed by principal component analysis. Strong correlations were highlighted among basic quality parameters, TPC, pigments, and OS. Simple linear regression was used to describe the relationships between OS and TPC (R² ​= ​0.856) and OS regressed against Chl (R² ​= ​0.690) and Car (R² ​= ​0.760), while TPC were regressed on Chl (R² ​= ​0.670) and Car (R² ​= ​0.680) and finally Chl against Car (R² ​= ​0.931). In conclusion, compared to technological, genotypic, and geographic effects, climatic conditions were the main factor driving olive oil stability and associated phenolics and pigments; oil cultivar blend seems to have negative effects on pigments concentration and total phenolic compounds as well as oxidative stability.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 255-264"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis and selection of high oleic peanut varieties in China: A study on agronomic, yield, and quality traits","authors":"Fangping Gong ,&nbsp;Zhuo Li ,&nbsp;Xiaojian Sun,&nbsp;Yi Fan,&nbsp;Yinghui Liu,&nbsp;Yurong Dang,&nbsp;Hao Li,&nbsp;Xingguo Zhang,&nbsp;Xingli Ma,&nbsp;Zhongfeng Li,&nbsp;Kai Zhao,&nbsp;Dongmei Yin","doi":"10.1016/j.ocsci.2024.09.001","DOIUrl":"10.1016/j.ocsci.2024.09.001","url":null,"abstract":"<div><div>Peanut (<em>Arachis hypogaea</em> L.) is an important oil crop. Oleic acid is a major factor that determines the quality of peanuts. Therefore, the high oleic and high oleic to linoleic acid ratio are the target traits in an advanced peanut breeding program. This study provided an extensive evaluation of the genetic and physical characteristics as well as disease resistance of 220 high oleic peanut varieties in China. Notably, these varieties clustered into five major categories based on their traits. A majority of these varieties have been bred using interspecific hybridization or selected from mutants of self-crossed parents, with the main parent varieties being Kaixuan 016 and CTWE. Analysis of disease resistance showed that most high oleic peanut varieties could resist two or three diseases. However, those varieties with resistance to multiple diseases were relatively scarce. Moreover, some high oleic peanut varieties showed no disease resistance or inadequate testing. The results further indicate that the genetic basis for high oleic peanut breeding is insufficient, highlighting the need for its further development. Importantly, our findings lay a critical foundation for future high oleic peanut breeding and promote better understanding of the genetic and trait diversity offered by these varieties.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 265-274"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142722030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of deacidification processes on the quality and oxidative stability of walnut oil","authors":"Xuan Ma ,&nbsp;Chongbo Huang ,&nbsp;Chang Zheng ,&nbsp;Fangyan Long ,&nbsp;Mandi Zhao ,&nbsp;Changsheng Liu","doi":"10.1016/j.ocsci.2024.10.001","DOIUrl":"10.1016/j.ocsci.2024.10.001","url":null,"abstract":"<div><div>In order to select an appropriate deacidification process and improve the quality of walnut oil, low-temperature cold-pressed crude walnut oil was used as raw material. Deacidified walnut oil was prepared using three deacidification processes: chemical deacidification (CD), adsorption deacidification (AD), and molecular distillation deacidification (MDD). The physicochemical properties, nutritional components, and <em>in vitro</em> antioxidant activities of the resulting deacidified walnut oils were comparatively analyzed. The results indicate that the fatty acid content in walnut oil exhibits fluctuating changes during the three different deacidification processes. The MDD shows a higher deacidification rate, reaching 94.06%, which is superior to the other two methods. Additionally, the AD retains more total phenols and tocopherols, with retention rates of 95.79% and 74.62%, respectively; whereas MDD is more effective at retaining phytosterols, achieving a retention rate of 98.09%. All these methods displayed positive impacts on the <em>in vitro</em> antioxidant capacity and oil stability of walnut oil, with ferric-reducing antioxidant power (FRAP) content and oxidative stability time were significantly reduced.whencompared to the untreated crude oil Among them, AD had the greatest impact on oxidative stability index (OSI), with its decreasing from 2.06 ​h to 0.82 ​h. Overall, compared to CD or MDD, the AD has best application prospects in preserving nutritional components.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 247-254"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142705440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid determination of oil content of single peanut seed by near-infrared hyperspectral imaging","authors":"Shunting Zhang ,&nbsp;Xue Li ,&nbsp;Du Wang ,&nbsp;Li Yu ,&nbsp;Fei Ma ,&nbsp;Xuefang Wang ,&nbsp;Mengxue Fang ,&nbsp;Huiying Lyu ,&nbsp;Liangxiao Zhang ,&nbsp;Zhiyong Gong ,&nbsp;Peiwu Li","doi":"10.1016/j.ocsci.2024.05.002","DOIUrl":"10.1016/j.ocsci.2024.05.002","url":null,"abstract":"<div><div>Oil content is a crucial indicator for evaluating the quality of peanuts. A rapid and non-destructive method to determine oil content of individual peanut seed can provide robust technical support for breeding high-oil-content peanut varieties. In this study, we established a rapid determination method using near-infrared hyperspectral imaging and chemometrics to assess the oil content of single peanut seed. After selecting key wavelengths through competitive adaptive reweighted sampling (CARS), uninformative variable elimination (UVE), and random frog (RF), we constructed an oil content calibration model based on partial least squares regression for single peanut seed. Validation results demonstrated that the correlation coefficient was 0.8393 with a root mean square error of 1.7771 in the calibration set, while it was 0.7915 with a root mean square error of 2.2943 in the independent prediction set. Most samples exhibited relative errors below 5%, confirming the reliability of this model in predicting oil content of single peanut seed.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 220-224"},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Solvent-free synthesis of diacylglycerols via enzymatic glycerolysis between edible oils and glycerol catalyzed by self-made immobilized lipase PS@LXTE-1000","authors":"Yuhao Li ,&nbsp;Yi Zhang ,&nbsp;Run Liu ,&nbsp;Zhonghui Liu ,&nbsp;Kheng-Lim Goh ,&nbsp;Vladimir Zivkovic ,&nbsp;Mingming Zheng","doi":"10.1016/j.ocsci.2024.05.003","DOIUrl":"10.1016/j.ocsci.2024.05.003","url":null,"abstract":"<div><div>Diglycerol (DAG) is a structural lipid with the functions to lower body fat accumulation and decrease serum triglyceride level. However, the enzymatic synthesis of DAG is limited by the high-efficient and economic lipases. In this paper, the immobilized lipase PS@LXTE-1000 was self-made by immobilizing the <em>Pseudomomas cepacian</em> lipase on to the hydrophobic microporous resin LXTE-1000. The results indicate that LXTE-1000 was a uniform mesoporous sphere with the mean diameter of 400 ​μm, pore size of 14.6 ​nm, pore volume of 0.5 ​cm³/g and surface area of 126.0 ​m²/g, showing superior structural properties for lipase immobilization. Under the optimal reaction conditions with the molar ratio of rapeseed oil to glycerol being 1:1, adding amount of immobilized lipase being 4%, reaction at 50 ​°C, the highest DAG content of 46.7% was achieved in 3 ​h via enzymatic glycerolysis catalyzed by LXTE-1000. After 7 cycles of reuse, the self-made LXTE-1000 could still retain 78.3% of its initial catalytic ability. Besides, LXTE-1000 was observed to facilitate the DAG production via glycerolysis reaction between glycerol with other seven edible oils including corn oil, sesame oil, peony seed oil, rice bran oil, peanut oil, soybean oil and flaxseed oil. Specifically, the glycerolysis reaction with sesame oil, peony seed oil and rice bran oil even led to the DAG content of 52.1%, 53.3% and 51.2%, respectively, Hence, this paper provide a novel strategy to produce high-efficient and economic immobilized lipases, which shows great potential in the green synthesis of functional lipids such as DAG.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":"Pages 225-233"},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142535347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of adding flaxseed milk coproduct and okara on the quality and glycemic response of Chinese steamed bread","authors":"Yan Tang ,&nbsp;Yaqiong Pei ,&nbsp;Jiahui Wang ,&nbsp;Haichao He ,&nbsp;Mingkai Sun ,&nbsp;Yashu Chen ,&nbsp;He Liu ,&nbsp;Hu Tang ,&nbsp;Qianchun Deng","doi":"10.1016/j.ocsci.2024.06.007","DOIUrl":"10.1016/j.ocsci.2024.06.007","url":null,"abstract":"<div><div>Flaxseed milk coproduct (FMC) is a by-product of flaxseed milk. Okara is a by-product of processed soybean products. In this study, we investigated the quality of dough and Chinese steamed bread (CSB) with the addition of FMC and okara. We also examined the in vitro starch digestibility, expected glycemic index (eGI), starch crystallinity, and short-range order structure of CSB. The results showed that FMC and okara decreased the dough fluidity, formed a dense structure, and enhanced the mechanical properties of the dough. FMC and okara increased the hardness, gumminess, and chewiness of the CSB, while decreasing its cohesion and elasticity. The addition of FMC and okara improved the nutrient content of CSB and reduced the eGI from 75.86 to 51.56. FMC and okara altered the multiscale structure of starch, effectively shielding the amylase site of action, and limited the interaction between amylase and starch. This study provides a reference for the high-value utilization of oilseed processing by-products.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Use of oilseed crops biomass for heavy metal treatment in water","authors":"Carlos Peña-Guzmán ,&nbsp;Ángela Otálvaro-Álvarez ,&nbsp;Tatiana Jiménez-Ariza","doi":"10.1016/j.ocsci.2024.07.001","DOIUrl":"10.1016/j.ocsci.2024.07.001","url":null,"abstract":"<div><p>The treatment of heavy metals in water is of high importance worldwide, and different treatment types have been developed. The use of plant material is becoming more and more important, and oilseed crops biomass have been investigated in terms of phytoremediation and biosorption processes. This article is a review of the literature reporting the applications in 10 different plants and evaluating the removal efficiencies for 12 metals, including the findings of 81 publications. <em>Moringa olifera</em> and <em>Helianthus annuus</em> are the most studied plants, whereas Cu (21.9%), Cd (18.5%), and Pb (19.9%) are the most studied metals. As a result, it was found that more than 90% of Pb, Cu, Cd, Fe, Zn, Ni, Cr, Sr and Mn showed removals in their experiments. At the same time, the variables most related to the efficiency of metal removal are pH, temperature, and contact time. This article includes a review of the biosorption isotherms used in the different studies.</p></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 3","pages":"Pages 177-186"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2096242824000447/pdfft?md5=9e225f18fee56a225807884ce6535ff6&pid=1-s2.0-S2096242824000447-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141847876","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sodium nitroprusside as a signal molecule for up-regulating membrane characteristics, antioxidant defense system to improve flax productivity under water stress","authors":"N.M. Al-Ashkar ,&nbsp;B.A. Bakry ,&nbsp;H.M.S. El-Bassiouny ,&nbsp;M.M.S. Abdallah ,&nbsp;M.S. Sadak","doi":"10.1016/j.ocsci.2024.07.002","DOIUrl":"10.1016/j.ocsci.2024.07.002","url":null,"abstract":"<div><p>Water stress is a critical environmental adversity that significantly impacts the growth, development, and yield of flax plants. In this study, flax seeds were cultivated under different water irrigation requirements (WIR) (100%, 75%, and 50%) to investigate the effects of exogenously supplied nitric oxide (NO) donor sodium nitroprusside (SNP) as foliar treatments at concentrations of 0.0 ​mmol/L, 0.5 ​mmol/L, 1.0 ​mmol/L, and 2.0 ​mmol/L. Drought stress led to a significant decrease in plant growth, photosynthetic pigments, yield components such as oil and total carbohydrate percentage. It also resulted in an increase in leaf H₂O₂ production, lipid peroxidation levels and activities of enzymatic antioxidants including polyphenol oxidase, superoxide dismutase, and nitrate reductase enzymes. However, foliar application of SNP improved photosynthetic pigments and antioxidant defense system which mitigated the negative impact of water stress on growth and yield productivity by reducing oxidative damage caused by reactive oxygen species accumulation. The use of SNP also decreased H₂O₂ accumulation levels, lipid peroxidation levels, and improved membrane stability. SNP treatment at concentration of 2 ​mmol/L showed superior results compared to other concentrations with extremely significant increases observed in yield characteristics such as oil content, total carbohydrate percentages, and unsaturated fatty acids to saturated fatty acids ratio.</p></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"9 3","pages":"Pages 160-169"},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2096242824000460/pdfft?md5=076919ec7711d6397968612ca3d1f8b9&pid=1-s2.0-S2096242824000460-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141848618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}