Oil Crop Science最新文献

{"title":"From field loss to yield security: Genetic pathways for building pod shattering-resistant rapeseed cultivars","authors":"Rijin Zhou ,&nbsp;Liying Zheng ,&nbsp;Jia Liu ,&nbsp;Wen Chu ,&nbsp;Qamar U. Zaman ,&nbsp;Ayub Ashfaq ,&nbsp;Hui Wang ,&nbsp;Qiong Hu ,&nbsp;Danyu Kong ,&nbsp;Hongtao Cheng","doi":"10.1016/j.ocsci.2026.01.005","DOIUrl":"10.1016/j.ocsci.2026.01.005","url":null,"abstract":"<div><div>Pod shattering, while a natural mechanism for seed dispersal, is an undesirable agronomic trait in rapeseed (<em>Brassica napus</em> L.) that complicates mechanical harvesting. It typically causes yield losses of 5%-15%, which can be further worsened under dry and hot conditions. As most of the modern rapeseed cultivars remain susceptible to shattering, enhancing pod shattering resistance (PSR) is important to safeguard global rapeseed production. Significant progresses have been made in elucidating the molecular and genetic mechanisms of silique dehiscence in the model plant <em>Arabidopsis</em> and pod shattering in rapeseed. This review firstly summarizes the genetic network controlling silique dehiscence in <em>Arabidopsis</em>, which is largely conserved in closely related <em>Brassica</em> species. We then synthesize discoveries from both forward and reverse genetic studies in rapeseed. Finally, the major challenges and future prospects in PSR research and breeding are discussed in depth.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 74-82"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147539221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rapid improvement of seed weight and yield by combining QTL pyramiding with speed breeding in Brassica napus L","authors":"Yixian Song ,&nbsp;Xin Yuan ,&nbsp;Pengfei Wang ,&nbsp;Zhaoyang Wang ,&nbsp;Lei Kang ,&nbsp;Jing Yang ,&nbsp;Guangsheng Yang ,&nbsp;Dengfeng Hong","doi":"10.1016/j.ocsci.2026.01.006","DOIUrl":"10.1016/j.ocsci.2026.01.006","url":null,"abstract":"<div><div>Thousand-seed weight (TSW) is a critical target for genetic improvement in rapeseed (<em>Brassica napus</em> L.). However, phenotypic selection for this trait remains challenging due to its polygenic regulation by multiple quantitative trait loci (QTL). Here, six favorable TSW QTL alleles from two donor parents were introgress into an elite restorer line, 621R, using an integrated strategy combining marker-assisted backcrossing and speed breeding protocols. Through six rounds of backcrossing and convergent crossing followed by two generations of selfing strategies, we developed 13 advanced lines with diverse TSW QTL combinations within 24 months. Field evaluations across three environments revealed that all lines exhibited significantly increased TSW in spring conditions (Minle, Gansu) and winter environments (Wuhan and Jiangling, Hubei) except for two lines which only showed increase in the spring environment. Hybridization assays using these lines as male parents crossed with two male-sterile lines (RG430A and 616A) demonstrated transgressive segregation for TSW: For RG430A-derived hybrids, all crosses significantly outperformed the original control (RG430A × 621R) in Wuhan, with 8/13 and 9/13 crosses showing significant TSW increases in Minle and Jiangling, respectively. For 616A-derived hybrids, 11/13 and 10/13 crosses exhibited significant TSW enhancement in Minle and Jiangling, compared to 3/13 in Wuhan. Notably, two top-performing hybrids achieved 13.0% and 6.8% higher plot yields, respectively. Our results demonstrate that strategic pyramiding of complementary TSW QTL alleles effectively enhances seed weight in rapeseed, and these improved lines represent valuable genetic resources for developing high-yield hybrids.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 83-91"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147539231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative mechanisms of sucrose and gibberellin in regulating soybean flowering","authors":"Kejing Zhang,&nbsp;Kai Luo,&nbsp;Yiling Li,&nbsp;Ping Lin,&nbsp;Jialin Yang,&nbsp;Yuze Li,&nbsp;WenYu Yang,&nbsp;Taiwen Yong","doi":"10.1016/j.ocsci.2026.01.002","DOIUrl":"10.1016/j.ocsci.2026.01.002","url":null,"abstract":"<div><div>As a pivotal global oil crop, soybean production plays a vital role in ensuring food security and promoting sustainable development. The processes of flowering and pod drop are critical determinants of soybean yield, thus, effective regulation of flowering is essential for achieving both high and stable yields. The differentiation of flower buds marks a crucial stage in the flowering process, governed by a complex interplay of environmental and endogenous pathways, including photoperiodic, vernalization, autonomous, gibberellin, and age pathways. These pathways converge to integrate flowering signals, subsequently activating downstream floral meristem identity genes that orchestrate the formation of floral primordia. Sucrose, the primary sugar transport form in soybeans, serves not only as a fundamental component of carbon metabolism but also as a significant signaling molecule. Through the age pathway, sucrose harmonizes various flowering-related genes, thereby influencing the timing of soybean flowering. Gibberellin, an essential hormone for plant growth and development, modulates flowering through the gibberellin pathway, with DELLA proteins acting as key regulators in the signal transduction cascade. The synergistic interaction between sucrose and gibberellin on gene expression occurs via distinct signaling pathways, collectively orchestrating flower bud differentiation. A thorough exploration of the molecular mechanisms by which sugars and hormones regulate flowering is anticipated to yield valuable insights and guidance for enhancing field crop production.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 103-111"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147539229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity in Morocco’ sunflower “Helianthus annuus L.” gene-bank for autumn-early winter plantation conditions: Agro-morpho-physiological screening","authors":"Karim Houmanat ,&nbsp;Abdelghani Nabloussi ,&nbsp;Atman Adiba ,&nbsp;Achraf Mamassi ,&nbsp;Hamid Mazouz ,&nbsp;Mohamed El Fechtali","doi":"10.1016/j.ocsci.2025.12.002","DOIUrl":"10.1016/j.ocsci.2025.12.002","url":null,"abstract":"<div><div>Despite Morocco's reliance on sunflower as an oilseed crop, little is known about its agronomic performance when sown in autumn or early winter. This knowledge gap is critical, as spring-sown varieties have shown declining performance in recent years under intensifying climate stress. Therefore, targeted breeding strategies could discover genotypes suitable for autumn or early winter sowing, with cold tolerance as a key selection criterion. Currently, ‘Ichraq’ is the only autumn-planted sunflower variety officially registered in Morocco, although efforts to release additional tolerant varieties are underway. This study evaluated 31 genotypes (MGB1 to MGB31) selected from various environments under autumn planting conditions and conserved in the Moroccan Gene Bank. These genotypes were planted in early winter at a mountainous site known for its pronounced winter cold. Eighteen Morphological, physiological and agronomic parameters including initial vigor, leaf area, seed yield, oil content etc., were assessed using both univariate and multivariate statistical approaches. Analysis of variance revealed significant genotypic differences across most traits, indicating substantial genetic variation. Notably, seed oil content ranged from 23.28% (MGB26) to 43.88% (MGB5), and seed yield from 1400 kg/ha (MGB7) to 5400 kg/ha (MGB8). Principal component analysis (PCA) identified that the first principal component, accounting for over 24% of the total phenotypic variance, exhibits a strong positive loading of yield-related traits and chlorophyll content, while displaying a pronounced negative loading for oil content variables. This opposing gradient indicates a clear trade-off between vegetative productivity and oil accumulation across the evaluated genotypes. Hierarchical cluster analysis resolved the germplasm into two principal clusters with high within-group similarity, each further partitioned into relatively homogeneous subgroups. Notably, several genotypes outperformed the control variety Ichraq, underscoring their potential for autumn or early winter cultivation. Nonetheless, essential multi-environment trials remain to validate their phenotypic stability and to ascertain their value as genetic resources for sunflower breeding programs in Morocco and other Mediterranean agro-ecosystems.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 9-19"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147400700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exogenous myo-inositol alleviates abiotic stress in rapeseed by modulating ion homeostasis and antioxidant defense","authors":"Shihang Fan ,&nbsp;Xulong Zhao ,&nbsp;Nian Liu ,&nbsp;Xiang Ji ,&nbsp;Zihong Huang ,&nbsp;Wei Hua ,&nbsp;Zhengwei Fu","doi":"10.1016/j.ocsci.2026.01.008","DOIUrl":"10.1016/j.ocsci.2026.01.008","url":null,"abstract":"<div><div>Soil salinization is a major abiotic stress that severely constrains global agricultural productivity. The application of exogenous bioactive substances represents a promising strategy to enhance crop salt tolerance. In this study, we investigated the protective role of exogenous <em>myo</em>-inositol in rapeseed under salinity stress. Here, we demonstrated that exogenous application of 20 μM <em>myo</em>-inositol significantly alleviates salt stress in rapeseed seedlings. <em>Myo</em>-inositol effectively mitigated growth inhibition, maintained chlorophyll levels and photosynthetic activity, and stabilized membrane integrity under salt stress. Physiological and molecular evidence indicated that <em>myo</em>-inositol activates the antioxidant system by enhancing the activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), thereby reducing reactive oxygen species accumulation. Notably, <em>myo</em>-inositol triggered a species-specific ion homeostasis strategy by increasing Na⁺ accumulation, associated with the upregulation of <em>BnHKT1</em> and downregulation of vacuolar <em>BnNHX</em> homologs. Concurrently, <em>myo</em>-inositol stimulated proline biosynthesis for osmotic adjustment. Furthermore, qRT-PCR analysis showed that <em>myo</em>-inositol fine-tunes the expression of key genes involved in antioxidant defense, osmotic adjustment, and stress signaling. These findings demonstrate that <em>myo</em>-inositol enhances rapeseed salt tolerance through an integrated mechanism involving antioxidant activation, transcriptional reprogramming, and a species-specific ion homeostasis strategy, establishing its potential as an effective biostimulant for saline agriculture.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 55-65"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147539220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Within-plant variability in pod maturity and preharvest sprouting in peanut (Arachis hypogaea L.): Physiological drivers, agronomic impacts, and integrated mitigation strategies","authors":"Yohannes Gelaye ,&nbsp;Huaiyong Luo","doi":"10.1016/j.ocsci.2026.01.007","DOIUrl":"10.1016/j.ocsci.2026.01.007","url":null,"abstract":"<div><div>Peanut (<em>Arachis hypogaea</em> L.) exhibits an unusually asynchronous reproductive cycle, in which flowering, peg penetration, pod development, and seed filling occur over an extended period. This results in the simultaneous presence of immature and preharvest sprouted (PHS) pods on the same plant a dual challenge that undermines yield, compromises seed quality, and complicates postharvest management. Immature pods reduce harvest efficiency, while PHS diminishes flavor, uniformity, and storage stability. Both genetic and environmental determinants ranging from temporal variation in peg initiation and hormonal gradients to microenvironmental heterogeneity and differential seed dormancy shape this variability. However, despite advances in pod biology, systematic field-based quantification of intra-plant temporal variation, genotype × environment interactions, and localized microclimatic influences remains limited. This review aims to synthesize current understanding of within-plant variability in pod maturation and PHS in peanut, to elucidate critical knowledge gaps at physiological and field scales, and to evaluate emerging strategies for mitigation. Particular emphasis is given for underexplored interface between physiological mechanisms and field-scale dynamics. Emerging innovations including hyperspectral imaging, soil and canopy moisture sensing, and molecular markers offer promising avenues for precise monitoring of pod maturity and early detection of PHS risk. Integrating these tools with targeted breeding strategies for synchronous flowering, enhanced dormancy, and late-season stress resilience, alongside adaptive agronomic practices such as optimized sowing, irrigation scheduling, nutrient management, and harvest timing, could substantially reduce yield and quality losses. Future progress will depend on bridging molecular insights with predictive models that capture mixed maturity and sprouting risk under variable environments.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 92-102"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147539223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive evaluation of seed iron content in peanut germplasm and identification of elite genotypes with high iron","authors":"Jianbin Guo ,&nbsp;Li Huang ,&nbsp;Huaiyong Luo,&nbsp;Weigang Chen,&nbsp;Bolun Yu,&nbsp;Xiaojing Zhou,&nbsp;Huifang Jiang,&nbsp;Boshou Liao,&nbsp;Yong Lei,&nbsp;Nian Liu","doi":"10.1016/j.ocsci.2026.01.004","DOIUrl":"10.1016/j.ocsci.2026.01.004","url":null,"abstract":"<div><div>Iron is an essential mineral element that plays important roles in plant growth, development, and human health. Peanut is a valuable source of iron for human nutrition. Improving iron content in peanut seeds can enhance both yield potential and nutritional value. In this study, the seed iron content of the 401 peanut germplasm accessions was estimated and substantial variation among these accessions was observed, ranging from 9.02 to 50.60 mg/kg. The seed iron content of <em>valencia</em> type accessions was significantly higher than that of <em>Peruvian</em>, <em>Virginia</em>, and <em>Irregular</em> types. Landraces showed the highest average iron content, followed by advanced cultivars, breeding lines and interspecific hybrid cultivars. Accessions with red seed coat exhibited significantly higher iron content compared with those with pink seed coat. Correlation analysis revealed that the seed iron content significantly negatively correlated with hundred seed weight (HSW), resveratrol and oleic acid. Eight accessions with high iron content were identified with an average iron content of 32.46 mg/kg, including two elite genotypes that Zh.h4280 showed high resveratrol levels (1057.34 μg/kg) and Zh.h1976 exhibited large seeds (HSW over 90g). Association analysis identified four markers, one of which, AHGS2053 stably explained with 5.75%–5.84% phenotypic variation. Accessions containing the favorable allele AHGS2053-250bp exhibited significantly higher iron content compared to those with alternative alleles. The results provide valuable germplasm resources and associated markers for breeding programs targeting high iron content in peanuts.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 38-44"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147539219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering climate-resilient and high-quality oilseed crops: The role of genomics, gene editing, and epigenetics","authors":"Hongju Sun ,&nbsp;Mingchao Xu ,&nbsp;Hui Liu ,&nbsp;Guijun Yan ,&nbsp;Weijun Zhou ,&nbsp;Sheng Chen ,&nbsp;Ling Xu ,&nbsp;Ahsan Ayyaz ,&nbsp;Yanhui Wang ,&nbsp;Zhanyuan Lu","doi":"10.1016/j.ocsci.2026.01.001","DOIUrl":"10.1016/j.ocsci.2026.01.001","url":null,"abstract":"<div><div>Modern oilseed breeding faces the complex challenge of simultaneously improving yield, nutritional quality, and stress resilience within a sustainable agricultural framework. A predictive, systems-oriented strategy offers a solution by using predictive modeling and precise genetic intervention to engineer target ideotypes. This review synthesizes the integration of genomic selection (GS), genome editing, and epigenetic regulation to operationalize a precision breeding strategy in major oilseed crops. We document how GS, utilizing high-density SNP arrays and sequencing data, has reduced breeding cycles by up to 50% in soybean, enabling rapid selection for complex traits like seed protein content. We highlight the precision of CRISPR-Cas systems in executing design goals, such as creating commercial-grade high-oleic soybeans (&gt;80% oleic acid) by knocking out <em>FAD2</em> genes. Similarly, editing glucosinolate biosynthesis genes in rapeseed has directly improved meal quality. Furthermore, we explore the emerging role of epigenetic regulation as a tunable layer in trait optimization, where DNA methylation patterns in sunflower are linked to drought stress memory and flowering time. Finally, we present an integrated molecular framework, which synergizes these technologies to develop ideotypes with optimized architectures and composition. Despite challenges in phenotyping and global regulation, the strategic implementation of this molecular toolkit is pioneering a new era of precision breeding for the sustainable intensification of oilseed production.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 28-37"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147539237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of rhizobial inoculation on soybean nodulation and rhizocompartment bacterial community under different soil conditions","authors":"Qin Han ,&nbsp;Ming Li ,&nbsp;Yuxuan Hong ,&nbsp;Ye Zhu ,&nbsp;Can Liu ,&nbsp;Xiaoqian Tang ,&nbsp;Qi Zhang ,&nbsp;Peiwu Li","doi":"10.1016/j.ocsci.2025.12.003","DOIUrl":"10.1016/j.ocsci.2025.12.003","url":null,"abstract":"<div><div>Rhizobial inoculation in soybean is an effective strategy in sustainable agriculture to reduce chemical fertilizer application and to increase crop production. It not only provides nitrogen sources for host plants but also improves the rhizosphere soil environment. However, the inoculation efficiency of rhizobia remains to be improved. In this study, we investigated the nodulation efficiency of <em>Bradyrhizobium</em> and <em>Sinorhizobium</em> strains under different soil conditions and evaluated their impacts on the rhizocompartment bacterial community. We found that inoculation with <em>Bradyrhizobium diazoefficiens</em> UASD 110 increased the number of soybean nodules in acidic soil, while <em>Sinorhizobium fredii</em> CCBAU 45436 was more effective in alkaline soil. However, inoculation with neither strain significantly affected nodulation in neutral soil. Then, we demonstrated that UASD 110 was more competitive in nodulation than CCBAU 45436, which was related to its higher abundance in the rhizosphere. Furthermore, we showed that while single inoculation with UASD 110 or CCBAU 45436 failed to alter the bacterial diversity, these two strains differentially influenced the rhizosphere microbial composition. Finally, we identified the main rhizosphere microorganisms that were affected by these two strains. Our findings revealed that the nodulation capacity of rhizobia and their colonization of rhizosphere and nodules are soil-type dependent, yet their impact on the rhizobacterial community exhibited consistent patterns. These findings provide valuable insights into optimizing rhizobial inoculation strategies to enhance nitrogen fixation efficiency.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 45-54"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147539217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Down-regulation of LACS8A03 gene in Brassica napus L. decrease the content of glucosinolates","authors":"Yulong Li ,&nbsp;Meiru Yang ,&nbsp;Chanyu Jin ,&nbsp;Wei Zhang ,&nbsp;Minqiang Tang ,&nbsp;Xingzhong Zhao ,&nbsp;Xiaoli Tan","doi":"10.1016/j.ocsci.2025.08.003","DOIUrl":"10.1016/j.ocsci.2025.08.003","url":null,"abstract":"<div><div>Long-chain acyl-CoA synthetase (LACS), belonging to the AMP-bound superfamily, catalyzes the activation of long-chain fatty acids to their coenzyme A esters. Candidate Gene Association analysis showed that LACS8 was not only related to the content of fatty acid, but also, surprisingly, to the content of glucosinolates. Here, we cloned <em>BnaLACS8A03</em> from rapeseed, and demonstrated its association with the glucosinolates content. We found that <em>BnaLACS8A03</em> was widely expressed in all tissues and organs, and significantly highly expressed in leaf and flower, which are the sites of glucosinolate synthesis. Bioinformatics predicted that it was located in peroxisome, where the β-oxidation of fatty acids mainly occurred, indicating that <em>BnaLACS8A03</em> was involved in the metabolism of fatty acids. Phenotypic studies of RNA interference (RNAi) lines revealed that down-regulation of <em>BnaLACS8A03</em> in rapeseed significantly reduced the glucosinolates content. In addition, the expression of glucosinolates biosynthesis-related genes and transport-related genes is altered in <em>BnaLACS8A03</em>-RNAi plants, suggesting that a possible crosstalk regulation mechanism exists in the regulation network of glucosinolate mechanism. Our results demonstrate that glucosinolates content can be manipulated by altering the expression of long chain acyl-Coenzyme A synthase gene <em>BnaLACS8A03</em>, which provide an available genetic resource for low glucosinolate breeding practice in rapeseed.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 20-27"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147539230","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}