Genome-wide analysis and stress-responsive expression profiling of the LEA (late embryogenesis abundant) gene family in wild peanut

Q3 Agricultural and Biological Sciences
Jiang Huanhuan , Chen Qiangbin , Wang Tong , Chen Gang
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引用次数: 0

Abstract

Late embryogenesis abundant (LEA) proteins generally accumulate in seeds during the later stages of maturation. Here we studied the LEA genes in two wild peanut species (Arachis duranensis and Arachis ipaensis) in an effort to create a genetic resource for peanut crop improvement. we identified 65 AdLEA and 69 AiLEA genes representing all 8 LEA subfamilies, which were unevenly distributed across 10 peanut chromosomes. The majority of LEA proteins were found to be highly hydrophilic. MEME analysis indicated that LEA gene motifs were conserved within groups, but not between groups. The LEA genes contained a diverse array of stress- and phytohormone-responsive cis-acting elements, with the AdLEA2-20 and AiLEA2-20 genes containing the greatest number of elements. Both AdLEA2-20 and AiLEA2-20 were upregulated in response to cold temperatures, drought, salinity, and abscisic acid exposure, although the dynamics were tissue-dependent. This study lays the foundation for future studies on the LEA gene family and abiotic stress in peanut, and our results will be invaluable for the genetic improvement of peanut by characterizing the genetic resources of wild peanut species.
野生花生LEA基因家族全基因组分析及应激响应表达谱分析
胚发育后期胚发育后期,种子中富集LEA蛋白。本文对两种野生花生(duranensis和ipaensis)的LEA基因进行了研究,以期为花生作物改良提供遗传资源。我们鉴定出65个AdLEA和69个AiLEA基因,代表了8个LEA亚家族,这些基因不均匀地分布在10条花生染色体上。大多数LEA蛋白被发现是高度亲水的。基因模因分析表明,LEA基因基序组内保守,组间不保守。LEA基因含有多种应激和植物激素响应的顺式作用元件,其中AdLEA2-20和AiLEA2-20基因含有最多的元件。AdLEA2-20和AiLEA2-20在低温、干旱、盐度和脱落酸暴露下均上调,尽管其动态是组织依赖的。本研究为今后花生LEA基因家族和非生物胁迫的研究奠定了基础,对野生花生品种遗传资源的鉴定和花生的遗传改良具有重要意义。
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来源期刊
Oil Crop Science
Oil Crop Science Food Science, Plant Science, Agronomy and Crop Science
CiteScore
3.40
自引率
0.00%
发文量
20
审稿时长
74 days
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