Oil Crop SciencePub Date : 2026-03-01Epub Date: 2026-02-07DOI: 10.1016/j.ocsci.2026.01.003
Huojiao Gan , Yan Li , Mingkai Sun , Yashu Chen , Dengfeng Peng , Xiaogang Luo , Hu Tang , Qianchun Deng
{"title":"Preparation and antioxidant application of TEMPO-oxidized flaxseed cellulose nanofibers gel films","authors":"Huojiao Gan , Yan Li , Mingkai Sun , Yashu Chen , Dengfeng Peng , Xiaogang Luo , Hu Tang , Qianchun Deng","doi":"10.1016/j.ocsci.2026.01.003","DOIUrl":"10.1016/j.ocsci.2026.01.003","url":null,"abstract":"<div><div>As the pace of urban life accelerates, plastic wrap has become an everyday necessity. However, traditional petroleum-based plastic wrap is difficult to degrade and prone to releasing harmful plasticizers. Therefore, developing sustainable, biodegradable, and high-performance alternative materials is crucial. Inspired by the cellulose-gum reinforcement mechanism in flaxseed hulls, this study utilized flaxseed hulls as raw material. Through hot water washing, alkali boiling, and bleaching, high-purity insoluble cellulose (FC) was extracted. Subsequently, flaxseed hull cellulose nanofibers (FCN) were prepared via TEMPO oxidation and ultrasonic treatment. Using FC and FCN, an all-natural cellulose-flaxseed gum composite membrane (CM) was constructed, where FCN serves as the framework and flaxseed gum acts as the binder, forming a dense structure. This composite membrane demonstrated effectiveness in nut preservation, significantly delaying nut oxidation and providing a viable pathway for sustainable food packaging.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 66-73"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147539321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Ameliorative effects of flaxseed-based plant milk on water restriction-induced constipation in mice","authors":"Congcong Ma, Lei Wang, Yashu Chen, Qianchun Deng, Qingde Huang, Jiqu Xu","doi":"10.1016/j.ocsci.2025.10.001","DOIUrl":"10.1016/j.ocsci.2025.10.001","url":null,"abstract":"<div><div>Traditional Chinese medicine has long regarded flaxseed as a natural remedy for constipation. However, the efficacy or mechanism of flaxseed plant milk (FPM), a functional beverage derived from flaxseed, in alleviating constipation has not yet been clearly established. In this study, network pharmacology was combined with <em>in viv</em>o experiments to evaluate the anti-constipation activity of FPM on water restriction-induced constipation mouse model. Compared with the model group, administration of FPM (100 mg/kg and 200 mg/kg) significantly shortened the time to first black stool excretion, increased number of fecal pellets and water content of fecal pellets, and improved Small Intestinal Transit Rate (<em>p <</em> 0.05). Histological analysis revealed that FPM (200 mg/kg) preserved colonic epithelial integrity, reduced lamina propria damage, and restored goblet cell populations. Serum biochemical assays showed that FPM decreased vasoactive intestinal peptide (VIP) levels and increased 5-hydroxytryptamine (5-HT) concentrations. Network pharmacology identified 214 overlapping targets between flaxseed bioactive compounds and constipation-related genes, with key hub targets including TP53, CASP3, TNF, and IL6. KEGG pathway enrichment suggested that the PI3K/AKT signaling pathway may serve as a central regulatory axis. Western blot further confirmed that p-PI3K and p-AKT protein levels in the colon were reduced in constipated mice, whereas FPM administration markedly restored their expression, indicating activation of the PI3K/AKT pathway. Overall, these findings demonstrate that FPM alleviates constipation by improving stool characteristics, enhancing intestinal motility, and maintaining mucosal integrity, potentially via modulation of the PI3K/AKT pathway. This study provides new insights into the gastrointestinal benefits of flaxseed-derived functional foods and supports their development as dietary supplements for intestinal health.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"11 1","pages":"Pages 1-8"},"PeriodicalIF":0.0,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146057510","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil Crop SciencePub Date : 2025-12-01Epub Date: 2025-12-15DOI: 10.1016/j.ocsci.2025.07.003
Julierme Zimmer Barbosa , Antonio Carlos Vargas Motta , Giovana Poggere , Stephen A. Prior
{"title":"Winners of the Brazilian soybean yield contest: climatic, soil, management, and economic factors","authors":"Julierme Zimmer Barbosa , Antonio Carlos Vargas Motta , Giovana Poggere , Stephen A. Prior","doi":"10.1016/j.ocsci.2025.07.003","DOIUrl":"10.1016/j.ocsci.2025.07.003","url":null,"abstract":"<div><div>Soybean yield contests have been generating useful information regarding the cultivation of this important crop commodity. The aim of this study was to analyze environmental, management, and economic variables associated with soybean yield contest winners in Brazil. Data from eleven summer soybean harvests included grain yield, rainfall, climatic and agronomic efficiency, soil chemical and physical attributes, management strategies, and economic indicators. Two grain yield classes were evaluated: 1) high yield (4967–6839 kg ha<sup>−1</sup>) and 2) very high yield (6859–8945 kg ha<sup>−1</sup>). Enhanced soybean yield was due to adequate rainfall that led to high climatic efficiency (77 %) and high agronomic efficiency; average values ranged between 78 % (high yield class) and 86 % (very high yield class). Correction and maintenance of soil chemical and physical quality (surface and subsurface), alternative fertilization and microbial co-inoculation were notable. Winners in the very high yield class reported higher soil K<sup>+</sup>, Ca<sup>2+</sup>, and Mg<sup>2+</sup> levels, more frequent use of dolomitic limestone, greater soil structural quality, greater use of biostimulants (via foliar spray), and higher net revenue. While high climatic efficiency was fundamental for success, intensive integration of technologies with conservation practices for better soil and crop management are necessary to maximize soybean yield and economic gains.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"10 4","pages":"Pages 294-302"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145885806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil Crop SciencePub Date : 2025-12-01Epub Date: 2025-11-04DOI: 10.1016/j.ocsci.2025.08.001
Xue Li , Du Wang , Li Yu , Fei Ma , Xuefang Wang , Liangxiao Zhang , Peiwu Li
{"title":"Multiple adulteration detection of olive oils by Raman spectroscopy","authors":"Xue Li , Du Wang , Li Yu , Fei Ma , Xuefang Wang , Liangxiao Zhang , Peiwu Li","doi":"10.1016/j.ocsci.2025.08.001","DOIUrl":"10.1016/j.ocsci.2025.08.001","url":null,"abstract":"<div><div>The increasing prevalence of multiple adulteration in olive oil affects product authenticity and market integrity. Therefore, it is necessary to develop advanced methods to detect the multiple adulteration. In this study, a portable Raman spectrometer was employed to address this issue, with adulterant mixtures using a D-optimal mixture design to ensure representative samples. The results showed that principal component analysis (PCA) was unable to distinguish authentic olive oils from adulterated ones, whereas partial least squares-discriminant analysis (PLS-DA) successfully differentiated the two groups. These findings demonstrated the technical feasibility of combining Raman spectroscopy with PLS-DA to detect multiple adulteration in olive oil, offering a promising method for portable, rapid authentication to counter increasingly sophisticated fraud in the edible oil industry.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"10 4","pages":"Pages 348-353"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil Crop SciencePub Date : 2025-12-01Epub Date: 2025-11-05DOI: 10.1016/j.ocsci.2025.04.009
Kunkun Zhao , Xufa Du , Pan Zhang, Jiakai Song, Peiyuan Du, Qikang Wang, Dongmei Yin
{"title":"Effects of sowing peanut with shells on key agronomic traits and yield","authors":"Kunkun Zhao , Xufa Du , Pan Zhang, Jiakai Song, Peiyuan Du, Qikang Wang, Dongmei Yin","doi":"10.1016/j.ocsci.2025.04.009","DOIUrl":"10.1016/j.ocsci.2025.04.009","url":null,"abstract":"<div><div>The peanut farming system plays a crucial role in the development of the agricultural industry. Traditionally, peanuts are sown as shelled seeds; however, systematic research on the agronomic performance and yield potential of planting with shells remains limited. In this study, we present a novel approach—peanut sowing with shells (P-S-S)—that promotes plant growth, enhances root development, and increases yield. This method also improves seed quality and elevates protein and oil content. Field trials conducted in the Huang-Huai-Hai region and northeastern China demonstrate the technique's broad adaptability, resulting in an average yield increase of 12.9%, with gains reaching up to 41% in northeastern areas, even under a 20% reduction in seeding rate. We have refined a standardized planting protocol for P-S-S based on the principle of “Two Reductions and Two Increases”: reduced input costs and post-harvest losses, along with increased production and economic benefits. This innovation contributes significantly to the advancement of peanut cultivation practices in China.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"10 4","pages":"Pages 271-278"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145799799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil Crop SciencePub Date : 2025-12-01Epub Date: 2025-12-15DOI: 10.1016/j.ocsci.2025.12.001
Yuting Zou , Rijin Zhou , Yinghai Xu , Mengyu Hao , Wenxiang Wang , Jia Liu , Wenliang Wei
{"title":"Overexpression of demethylase-related gene FTO improves salt and drought tolerance in rapeseed","authors":"Yuting Zou , Rijin Zhou , Yinghai Xu , Mengyu Hao , Wenxiang Wang , Jia Liu , Wenliang Wei","doi":"10.1016/j.ocsci.2025.12.001","DOIUrl":"10.1016/j.ocsci.2025.12.001","url":null,"abstract":"<div><div>Drought and salt stresses are major abiotic factors that severely affect the growth, development, and yield formation of <em>Brassica napus</em>. Human derived <em>FTO</em> gene (Fat mass and obesity-associated), is a member of the AlkB family, encoding a mRNA demethylase that is dependent on α-ketoglutarate. Although previous studies have demonstrated that exogenous overexpression of <em>FTO</em> gene can increase plant biomass, its impact on plant stress resistance is still unclear. In this study, we cloned the <em>FTO</em> gene and conducted an analysis of its biological functions for drought and salt resistance for <em>Brassicaceae</em> plants. By overexpressing the <em>FTO</em> gene in <em>Arabidopsis thaliana</em>, the inhibitory effect of salt and drought stress on the root length growth of transgenic lines was significantly lower than that of the control. Moreover, the overexpression of <em>FTO</em> markedly enhanced the tolerance of <em>Arabidopsis</em> to drought and salt stress. It also led to a decrease in malondialdehyde (MDA) content, an increase in proline content, and a boost in superoxide dismutase (SOD) activity. Meanwhile, when the <em>FTO</em> gene was heterologously expressed in <em>B. napus</em>, the transgenic plants were less affected by stress. In comparison to control plants, they exhibited significantly lower MDA levels and markedly higher proline content and SOD activity. Furthermore, staining results with Trypan blue and nitroblue tetrazolium (NBT) staining indicate that the <em>FTO</em> gene can alleviate the damage to plants under stress and inhibit the accumulation of O<sub>2</sub><sup>−</sup>. Comprehensively, the results indicate that overexpression of the <em>FTO</em> gene can improve the drought and salt tolerance in transgenic plants, providing valuable references for further exploring the <em>FTO</em>-mediated stress resistance mechanisms.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"10 4","pages":"Pages 311-320"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145885807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil Crop SciencePub Date : 2025-12-01Epub Date: 2025-12-15DOI: 10.1016/j.ocsci.2025.11.001
Dan Wang , Jinghan Miao , Hua Zhang , Hong Chen , Fang Wei
{"title":"The impact of advanced rapeseed pretreatment technology on minor compounds in rapeseed oil: A review","authors":"Dan Wang , Jinghan Miao , Hua Zhang , Hong Chen , Fang Wei","doi":"10.1016/j.ocsci.2025.11.001","DOIUrl":"10.1016/j.ocsci.2025.11.001","url":null,"abstract":"<div><div>Rapeseed is the primary domestic source of edible vegetable oil in China. Its oil is naturally rich in phytosterols, polyphenols, tocopherols, carotenoids and other lipid-soluble companions, which are highly beneficial to human health. Since the content and bioaccessibility of these health-promoting companions are mostly locked during the first post-harvest operation, the pretreatment technology has become the decisive lever for simultaneously maximizing oil yield and nutritional value. This review critically synthesizes recent findings on how rapeseed pretreatment modulates its signature lipid companions, contrasts conventional roasting with emerging pulsed-electric field, microwave and steam-explosion technologies, and evaluates their respective impacts on extraction efficiency, structural integrity and functional performance on phytosterols, polyphenols, tocopherols and carotenoids. It particularly compares and analyzes the extraction efficiency, cost-effectiveness, and environmental impact for these emerging technologies, and discusses the prospects for their integrated applications. Furthermore, the review concludes with the current research trends in pretreatment technology and offers insights into future research directions, aiming to provide scientific guidance for the high-quality processing of rapeseed and the production of rapeseed oil with high nutritional value.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"10 4","pages":"Pages 303-310"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145885817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil Crop SciencePub Date : 2025-12-01Epub Date: 2025-12-19DOI: 10.1016/j.ocsci.2025.08.002
Habtamu Kefale , Rong Zhou , Muez Berhe , Ahmed A. Abbas , Chris O. Ojiewo , Huan Li , Ting Zhou , Jun You , Linhai Wang
{"title":"Genome-wide association study (GWAS) identifies genetic loci and candidate genes for variation in protein and oil content in Sesamum indicum L.","authors":"Habtamu Kefale , Rong Zhou , Muez Berhe , Ahmed A. Abbas , Chris O. Ojiewo , Huan Li , Ting Zhou , Jun You , Linhai Wang","doi":"10.1016/j.ocsci.2025.08.002","DOIUrl":"10.1016/j.ocsci.2025.08.002","url":null,"abstract":"<div><div>Sesame (<em>Sesamum indicum</em> L.) is an ancient diploid oilseed crop valued for its high oil content, quality protein, unsaturated fatty acids, and antioxidants. The emerging research focuses on the utilization of vegetable proteins in the development of novel products and the formulation of functional foods. Developing sesame genotypes with higher protein and oil content is a current focus for improving sesame quality traits, as preferred by the sesame seed oil industry and consumers. Despite previous genome-wide association studies (GWAS) on various agronomic and biochemical traits in sesame, the genetic basis of protein content and oil content remains limited. Therefore, this study was designed to identify candidate genes and quantitative trait loci (QTLs) regulating the target traits using 401 sesame accessions grown in three environments (2018WH, 2018LQ, and 2022WH). The protein and oil content in the sesame population varied significantly. The protein content ranged from 15.06% to 26.45%, while the oil content was between 41.68% and 60.15%. Furthermore, the white seeds had a higher oil content, while the black seeds had a higher protein content, indicating an association between seed colour and oil and protein content. GWAS results revealed that 632 and 269 QTLs were selected as significant genetic regions for protein and oil content, respectively. Of these significant QTLs, we identified 10 and 22 stable QTLs for protein and oil content, respectively. A total of 17 candidate genes related to protein and oil content were identified within 32 stable quantitative trait loci (QTLs). Interestingly, two variant sites, SNP13:10093594 and InDel13:10935497, related to protein content, were identified in the gene sequences of <em>SINPZ1300586</em> and <em>SINPZ1300694</em>. Four SNPs, SNP1:22608987, SNP4:13355820, SNP8:21686488, and SNP11:142842, for oil content were found correspond to the genes <em>SINPZ0102106, SINPZ0401222, SINPZ0801731,</em> and <em>SINPZ1100015</em>, respectively. The findings provide light on the genetic basis of protein and oil characteristics. These genes and QTLs can aid the molecular breeding and genetic engineering of innovative sesame germplasm, with improved potential for protein and oil content, as well as desired quality attributes.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"10 4","pages":"Pages 337-347"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145927602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development of novel genotypes of peanut with resistance to stem rot, large pod and seed","authors":"Wanduo Song, Dongyang Yu, Yanping Kang, Qianqian Wang, Yong Lei, Zhihui Wang, Dongxin Huai, Xin Wang, Boshou Liao, Yuning Chen, Liying Yan","doi":"10.1016/j.ocsci.2025.07.001","DOIUrl":"10.1016/j.ocsci.2025.07.001","url":null,"abstract":"<div><div>Stem rot caused by <em>Agroathelia rolfsii</em> (syn. <em>Sclerotium rolfsii</em>) is one of the major biotic constraints to peanut production in many countries, particularly under high temperature and humidity conditions. Developing disease-resistant cultivars represents a more sustainable strategy. To generate peanut germplasm integrating stem rot resistance and elite yield traits, this study utilized a recombinant inbred line (RIL) population, consisting of 242 lines, derived from a cross between Zhonghua212 (medium-seeded, resistant parent), and Zhonghua21 (large-seeded, susceptible parent). A multi-environment evaluation was conducted for disease resistance profiling in the fields with artificial inoculation, and for yield-related traits assessment. The results indicated that twenty-two RIL lines exhibited consistent moderate resistance across all tested locations. Fifty RILs consistently expressed large pod (100-pod weight >180 g) and large seed (100-seed weight >80 g) phenotypes in two environments. Three elite RIL lines (BJF66, BJF119 and BJF137) combined moderate resistance with superior pod/seed traits, providing valuable genetic sources for breeding programs targeting both disease resistance and productivity. This study established a foundational germplasm pool for advancing stem rot resistant, high-yielding peanut varieties, aligning with sustainable agricultural practices to mitigate <em>A. rolfsii</em> threats.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"10 4","pages":"Pages 279-285"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145842553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Oil Crop SciencePub Date : 2025-12-01Epub Date: 2025-11-04DOI: 10.1016/j.ocsci.2025.05.004
Sichen Guan , Jin Mao , Qiaomei Qin , Na Li , Ling Cheng , Xianglong Yang , Qi Zhang , Peiwu Li
{"title":"Effect of ARC-BBBE microbial inoculant on main phytohormones in peanut root from seedling to podding stage","authors":"Sichen Guan , Jin Mao , Qiaomei Qin , Na Li , Ling Cheng , Xianglong Yang , Qi Zhang , Peiwu Li","doi":"10.1016/j.ocsci.2025.05.004","DOIUrl":"10.1016/j.ocsci.2025.05.004","url":null,"abstract":"<div><div>Phytohormones play a crucial role in regulating peanut growth and development. Our previous studies have demonstrated that the microbial inoculant ARC-BBBE, developed by our research group, effectively promotes peanut growth and enhances yield under both greenhouse and field conditions. Therefore, it is of significant interest to investigate how ARC-BBBE influences the levels and spatial distribution of major phytohormones in peanut roots. Greenhouse pot experiments revealed that ARC-BBBE significantly enhanced peanut growth and root system development. A systematic analysis of the effects of ARC-BBBE on key phytohormones in peanut roots across different growth stages showed that gibberellin A<sub>3</sub> (GA<sub>3</sub>) content varied markedly, with predominant accumulation occurring during the early growth stage, whereas changes in indole-3-acetic acid (IAA) levels were not statistically significant. Specifically, GA<sub>3</sub> content in the ARC-BBBE treatment group was 1.27-fold higher than in the control group during the seedling stage. Furthermore, peanut growth parameters were significantly improved following ARC-BBBE application, particularly at the flowering stage, where plant height, above-ground biomass, root length, and root weight in the treated group were 1.24-, 1.17-, 1.13-, and 1.21-fold greater than those in the control, respectively. To elucidate the functional role of phytohormones in ARC-BBBE-mediated growth promotion, we examined the effects of exogenous GA<sub>3</sub> and its biosynthesis inhibitor uniconazole (S3307) on both PHNZY-23-3 rhizobial growth and peanut development. Results indicated that supplementation with 1 × 10<sup>3</sup> mg/L GA<sub>3</sub> most effectively promoted peanut growth at the seedling stage, while S3307 application inhibited growth. These findings provide valuable insights into the mechanism by which ARC-BBBE modulates GA<sub>3</sub> dynamics to enhance peanut growth, offering a foundation for future research on plant-microbe interactions and phytohormone regulation.</div></div>","PeriodicalId":34095,"journal":{"name":"Oil Crop Science","volume":"10 4","pages":"Pages 330-336"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145885819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}