ECORFAN Journal Republic of Guatemala最新文献_第2页

Effects of stereotactic surgery on the anterior hypothalamus (HA) on the estrous cycle: Role of the dopaminergic system in spontaneous ovulation in the rat 立体定向手术对下丘脑前部(HA)在发情周期中的影响:多巴胺能系统在大鼠自发排卵中的作用

ECORFAN Journal Republic of Guatemala Pub Date : 2019-06-30 DOI: 10.35429/ejrg.2019.8.5.27.50

José Luis Morán-Perales, Octavio Sánchez-García, W. A. García-Suástegui, Anabella Handal-Silva

{"title":"Effects of stereotactic surgery on the anterior hypothalamus (HA) on the estrous cycle: Role of the dopaminergic system in spontaneous ovulation in the rat","authors":"José Luis Morán-Perales, Octavio Sánchez-García, W. A. García-Suástegui, Anabella Handal-Silva","doi":"10.35429/ejrg.2019.8.5.27.50","DOIUrl":"https://doi.org/10.35429/ejrg.2019.8.5.27.50","url":null,"abstract":"We evaluated the function of dopaminergic receptors (DAR) of the anterior hypothalamus (AH) on the estral cycle (EC) regulation and spontaneous ovulation by a single microinjection (MI) with the dopaminergic antagonist haloperidol (HLP) in adult rats. One hundred thirty nine rats that exhibit forth-day estral cycles (cyclic animals: CA) received a stereotaxic surgery (STXS) on the right, left or both AH sides and were distributed in three different groups with a MI of 1 µL of: HLP (15 µg) or dimethyl-sulfoxide (vehicle) or other false MI group. All the animals with STXS were sacrificed in next vaginal estrus (VE) exhibited and the ova shed (OS) counted. In sixteen AC, the OS were counted at VE and forming a control group. The STXS affected the animals EC: just 59/139 exhibited a short EC (SEC) with 4.6±0.1 days compared with 80/139 that exhibited a long EC (LEC) of 13.6±0.2 days. False or HLP MI diminished OS just in animals exhibiting a SEC. STXS affects neuroendocrine processes controlling EC length when cutting dorsal connections to AH. The DAR of the AH participate on ovarian mechanisms of follicular selection.","PeriodicalId":339379,"journal":{"name":"ECORFAN Journal Republic of Guatemala","volume":"100 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120906645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phylogenetic analysis of Na+/H+ (NuoL/MrpA) antiporters Na+/H+ (NuoL/MrpA)反转运蛋白的系统发育分析

ECORFAN Journal Republic of Guatemala Pub Date : 2019-06-30 DOI: 10.35429/ejrg.2019.8.5.8.15

L. Sánchez-Calderón, M. Chávez-Avilés, A. L. Díaz-Pérez, B. E. Gómez-Luna, J. C. Ramírez-Granados, R. Veloz-García, C. Díaz‐Pérez

{"title":"Phylogenetic analysis of Na+/H+ (NuoL/MrpA) antiporters","authors":"L. Sánchez-Calderón, M. Chávez-Avilés, A. L. Díaz-Pérez, B. E. Gómez-Luna, J. C. Ramírez-Granados, R. Veloz-García, C. Díaz‐Pérez","doi":"10.35429/ejrg.2019.8.5.8.15","DOIUrl":"https://doi.org/10.35429/ejrg.2019.8.5.8.15","url":null,"abstract":"Objectives: Sodium/proton (Na+/H+) antiporters NuoL/MrpA-like proteins are important in monovalent cations homeostasis, ATP synthesis, are involved in growth using low concentrations of acetate, and in management of protons during methane production. To learn more about the evolutive origin and biological relevance of this protein, in this work a phylogenetic analysis of the NuoL/MrpA superfamily of proteins was done. Methodology: Phylogeny reconstruction was done with 596 NuoL/MrpA proteins and 39 MrpD-NuoM/N proteins. The algorithms used were minimum evolution and maximum likelihood, using MEGA program. Additionally, a conserved domain analysis was done. Contribution: NuoL/MrpA superfamily and their homologous proteins, MrpD-NuoM/N, form two paralogous groups. The NuoL/MrpA superfamily consists of two families. Family NuoL consist of arqueal, bacterial and eukaryotic proteins of around 600 aa in size. Family MrpA are formed by proteins from bacteria and archaea, with a 600 to 850 aa in size. Using the phylogenetic analysis and conserved domain analysis, a superfamily NuoL/MrpA evolution model was proposed.","PeriodicalId":339379,"journal":{"name":"ECORFAN Journal Republic of Guatemala","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129008413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Determination of the microbiological load in organic, industrial and transfer type eggs in the central-west region of the State of Veracruz 韦拉克鲁斯州中西部地区有机、工业和转移型鸡蛋中微生物负荷的测定

ECORFAN Journal Republic of Guatemala Pub Date : 2019-06-30 DOI: 10.35429/ejrg.2019.8.5.16.26

Magdalena Jimenez-Hernandez, Noemí Nava-Valente, O. A. Del Ángel-Coronel, Rocío Frias-Frias

{"title":"Determination of the microbiological load in organic, industrial and transfer type eggs in the central-west region of the State of Veracruz","authors":"Magdalena Jimenez-Hernandez, Noemí Nava-Valente, O. A. Del Ángel-Coronel, Rocío Frias-Frias","doi":"10.35429/ejrg.2019.8.5.16.26","DOIUrl":"https://doi.org/10.35429/ejrg.2019.8.5.16.26","url":null,"abstract":"El objetivo del presente trabajo fue determinar la carga microbiológica en huevo de traspatio, tipo orgánico e industrial de la región centro-occidente del estado de Veracruz. Se evaluó la presencia de microorganismos patógenos en los tres sistemas de producción y se determinó la calidad microbiológica de cada uno de estos sistemas. Lo cual es de suma importancia considerando que en huevo con alta carga microbiológica, pueden estar presentes microorganismos como Salmonella spp S. enteritidis, etc., los cuales son considerados agentes causales de infecciones entéricas en la población. Aunado a lo anterior, los sistemas de explotación antes mencionados, representan una fuente de ingreso económico relevante para las familias que habitan esta región. Para el análisis microbiológico, se tomaron diferentes muestras de cascaron y de la parte interna (clara y yema). Se evaluó la presencia de Salmonella spp aplicando el procedimiento establecido en la Norma Oficial Mexicana NOM-114-SSA1-1994. Además se realizaron pruebas físicas para evaluar si los defectos físicos en huevo pueden correlacionarse con la presencia de Salmonella spp u otras bacterias. Contribución: Actualmente es poca la información que existe sobre la calidad microbiológica, en huevo proveniente de los diferentes sistemas de explotación que se consumen en la región centro-occidente del estado de Veracruz, particularmente para el sistema orgánico y de traspatio.","PeriodicalId":339379,"journal":{"name":"ECORFAN Journal Republic of Guatemala","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121709460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antiparasitic activity of Ophiocomina nigra in Entamoeba invadens 黑麻霉对入侵内阿米巴虫的抗寄生活性

ECORFAN Journal Republic of Guatemala Pub Date : 2019-06-30 DOI: 10.35429/ejrg.2019.8.5.1.7

S. Sánchez-Ramos, Laura Valdés-Santiago, J. P. Castruita-Domínguez, J. Villagómez-Castro

引用次数: 0