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Proceedings of the European Light Microscopy Initiative 2021最新文献

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The Image Data Resource: a scalable resource for FAIR biological imaging data 图像数据资源:FAIR生物成像数据的可扩展资源
Proceedings of the European Light Microscopy Initiative 2021 Pub Date : 2021-06-22 DOI: 10.22443/rms.elmi2021.73
Frances Wong
{"title":"The Image Data Resource: a scalable resource for FAIR biological imaging data","authors":"Frances Wong","doi":"10.22443/rms.elmi2021.73","DOIUrl":"https://doi.org/10.22443/rms.elmi2021.73","url":null,"abstract":"","PeriodicalId":334941,"journal":{"name":"Proceedings of the European Light Microscopy Initiative 2021","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122567159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Review of Effective Ways for Computing Point Spread Functions 计算点扩展函数的有效方法综述
Proceedings of the European Light Microscopy Initiative 2021 Pub Date : 2021-06-22 DOI: 10.22443/rms.elmi2021.49
R. H. D. Miora
{"title":"Review of Effective Ways for Computing Point Spread Functions","authors":"R. H. D. Miora","doi":"10.22443/rms.elmi2021.49","DOIUrl":"https://doi.org/10.22443/rms.elmi2021.49","url":null,"abstract":"","PeriodicalId":334941,"journal":{"name":"Proceedings of the European Light Microscopy Initiative 2021","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125997659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Computational tool for automated study of cell division dynamics in 3D cellular spheroids 用于三维细胞球体中细胞分裂动力学自动研究的计算工具
Proceedings of the European Light Microscopy Initiative 2021 Pub Date : 2021-06-22 DOI: 10.22443/rms.elmi2021.60
Stylianos Didaskalou
{"title":"Computational tool for automated study of cell division dynamics in 3D cellular spheroids","authors":"Stylianos Didaskalou","doi":"10.22443/rms.elmi2021.60","DOIUrl":"https://doi.org/10.22443/rms.elmi2021.60","url":null,"abstract":"","PeriodicalId":334941,"journal":{"name":"Proceedings of the European Light Microscopy Initiative 2021","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127633977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A versatile and highly customisable platform optimised for live high/super-resolution imaging of invading multicellular tumour spheroids in 3D collagen matrices 一个多功能和高度可定制的平台，优化了三维胶原基质中入侵多细胞肿瘤球体的高/超分辨率成像
Proceedings of the European Light Microscopy Initiative 2021 Pub Date : 2021-06-22 DOI: 10.22443/rms.elmi2021.27
Tom Phillips
{"title":"A versatile and highly customisable platform optimised for live high/super-resolution imaging of invading multicellular tumour spheroids in 3D collagen matrices","authors":"Tom Phillips","doi":"10.22443/rms.elmi2021.27","DOIUrl":"https://doi.org/10.22443/rms.elmi2021.27","url":null,"abstract":"","PeriodicalId":334941,"journal":{"name":"Proceedings of the European Light Microscopy Initiative 2021","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114661264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A close-up view of mitophagy using mt-keima and fluorescence lifetime microscopy (FLIM) 利用mt-keima和荧光寿命显微镜(FLIM)近距离观察线粒体自噬
Proceedings of the European Light Microscopy Initiative 2021 Pub Date : 2021-06-22 DOI: 10.22443/rms.elmi2021.4
D. Malide
{"title":"A close-up view of mitophagy using mt-keima and fluorescence lifetime microscopy (FLIM)","authors":"D. Malide","doi":"10.22443/rms.elmi2021.4","DOIUrl":"https://doi.org/10.22443/rms.elmi2021.4","url":null,"abstract":"Mitophagy is a cellular process that selectively removes damaged, old or dysfunctional mitochondria. Defective mitophagy is thought to contribute to normal aging and to various neurodegenerative and cardiovascular diseases. Previous methods used to detect mitophagy in vivo were cumbersome, insensitive and difficult to quantify. We created a transgenic mouse model that expresses the pH-dependent fluorescent protein mt-Keima in order to more readily assess mitophagy. Keima is a pH-sensitive, dual-excitation ratiometric fluorescent protein that also exhibits resistance to lysosomal proteases. At the physiological pH of the mitochondria (pH 8.0), the shorter-wavelength excitation predominates. Within the acidic lysosome (pH 4.5) after mitophagy, mt-Keima undergoes a gradual shift to longerwavelength excitation [1, 2]. In addition to intensity imaging we describe here how to apply mt-Keima fluorescence lifetime microscopy (FLIM) to visualize mitophagy in live cells as well as various tissues including skeletal muscle, heart, liver, and kidney, obtained from mtKeima transgenic mice. We observed that in control live cells mt-Keima fluorescence exhibits two components a short (0.4ns) lifetime corresponding to the mitophagic compartment and a longer (2.6ns) lifetime corresponding to normal mitochondria, in good correspondence to the intensity images. Interestingly, in the tissues the lifetime measurements reveal a heterogeneous mitophagic compartment containing in addition to the short (0.5ns) lifetime mt-Keima species an intermediary (1.2ns) longer lifetime component. Whether these 2 components correspond to different folding states, digestion products of the mt-Keima in the acidic environment remains to be elucidated. In conclusion FLIM provide a complementary approach to asses mitophagy in normal cells and tissues as well as in disease situations, or altered under environmental, genetic perturbations, or in aging.","PeriodicalId":334941,"journal":{"name":"Proceedings of the European Light Microscopy Initiative 2021","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125078152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
PYCALIBRATE: FULLY AUTOMATED PSF ANALYSIS Pycalibrate:全自动PSF分析
Proceedings of the European Light Microscopy Initiative 2021 Pub Date : 2021-06-22 DOI: 10.22443/rms.elmi2021.11
A. Corbett
{"title":"PYCALIBRATE: FULLY AUTOMATED PSF ANALYSIS","authors":"A. Corbett","doi":"10.22443/rms.elmi2021.11","DOIUrl":"https://doi.org/10.22443/rms.elmi2021.11","url":null,"abstract":"STANDARDISING IMAGE ANALYSIS: One of the key barriers to data reproducibility is the lack of standardization in microscope quality control (QC). Microscope QC requires standardization of both the sample used to measure microscope performance and the software used to analyse the images acquired. Despite there being several commercial (e.g. SVI Huygens) and freely available (PSFJ, MetroloJ) software solutions available there is currently no single package that is widely used by the microscopy community. Moreover, the solutions that do exist are semi-automated, requiring user input to define acquisition parameters and providing a window for error. This lack of automation and standardization makes performance comparisons between different microscopes unreliable.","PeriodicalId":334941,"journal":{"name":"Proceedings of the European Light Microscopy Initiative 2021","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127892506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
True server-side volumetric 3D animations created remotely with 3Dscript using a natural-language-based syntax 使用基于自然语言的语法使用3Dscript远程创建的真正的服务器端体积3D动画
Proceedings of the European Light Microscopy Initiative 2021 Pub Date : 2021-06-22 DOI: 10.22443/rms.elmi2021.19
Benjamin Schmid
{"title":"True server-side volumetric 3D animations created remotely with 3Dscript using a natural-language-based syntax","authors":"Benjamin Schmid","doi":"10.22443/rms.elmi2021.19","DOIUrl":"https://doi.org/10.22443/rms.elmi2021.19","url":null,"abstract":"","PeriodicalId":334941,"journal":{"name":"Proceedings of the European Light Microscopy Initiative 2021","volume":"92 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126333025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Correlative microscopy of samples of different topologies between light and electron microscopes 光学显微镜和电子显微镜之间不同拓扑样品的相关显微镜
Proceedings of the European Light Microscopy Initiative 2021 Pub Date : 2021-06-22 DOI: 10.22443/rms.elmi2021.92
Joerg E. Wissler
{"title":"Correlative microscopy of samples of different topologies between light and electron microscopes","authors":"Joerg E. Wissler","doi":"10.22443/rms.elmi2021.92","DOIUrl":"https://doi.org/10.22443/rms.elmi2021.92","url":null,"abstract":"","PeriodicalId":334941,"journal":{"name":"Proceedings of the European Light Microscopy Initiative 2021","volume":"57 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131301247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Real-Time Monitoring of Wound Healing by Using Label-free Multiphoton Microscopy and the 3D Printed Live-cell Imaging Chamber 利用无标签多光子显微镜和3D打印活细胞成像室实时监测伤口愈合
Proceedings of the European Light Microscopy Initiative 2021 Pub Date : 2021-06-22 DOI: 10.22443/rms.elmi2021.43
Che-Wei Chang
{"title":"Real-Time Monitoring of Wound Healing by Using Label-free Multiphoton Microscopy and the 3D Printed Live-cell Imaging Chamber","authors":"Che-Wei Chang","doi":"10.22443/rms.elmi2021.43","DOIUrl":"https://doi.org/10.22443/rms.elmi2021.43","url":null,"abstract":"","PeriodicalId":334941,"journal":{"name":"Proceedings of the European Light Microscopy Initiative 2021","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133207203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Fast Projection Imaging Method for the Quantification of the Dynamics of Endosome Maturation 内核体成熟动力学定量的快速投影成像方法
Proceedings of the European Light Microscopy Initiative 2021 Pub Date : 2021-06-22 DOI: 10.22443/rms.elmi2021.74
Xian Hu
{"title":"A Fast Projection Imaging Method for the Quantification of the Dynamics of Endosome Maturation","authors":"Xian Hu","doi":"10.22443/rms.elmi2021.74","DOIUrl":"https://doi.org/10.22443/rms.elmi2021.74","url":null,"abstract":"","PeriodicalId":334941,"journal":{"name":"Proceedings of the European Light Microscopy Initiative 2021","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121130267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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