PYCALIBRATE: FULLY AUTOMATED PSF ANALYSIS

Abstract

STANDARDISING IMAGE ANALYSIS: One of the key barriers to data reproducibility is the lack of standardization in microscope quality control (QC). Microscope QC requires standardization of both the sample used to measure microscope performance and the software used to analyse the images acquired. Despite there being several commercial (e.g. SVI Huygens) and freely available (PSFJ, MetroloJ) software solutions available there is currently no single package that is widely used by the microscopy community. Moreover, the solutions that do exist are semi-automated, requiring user input to define acquisition parameters and providing a window for error. This lack of automation and standardization makes performance comparisons between different microscopes unreliable.